scholarly journals BAC-Dkk3-EGFPTransgenic Mouse: AnIn VivoAnalytical Tool forDkk3Expression

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Yuki Muranishi ◽  
Takahisa Furukawa
Keyword(s):  
Transgenic Mice ◽  
Glial Cells ◽  
Wnt Signaling Pathway ◽  
Mouse Retina ◽  
Retinal Regeneration ◽  
Adult Retina ◽  
Müller Glial Cells ◽  
Mature Mouse ◽  
Precise Expression ◽  
Embryonic Retina

Dickkopf (DKK) family proteins are secreted modulators of the Wnt signaling pathway and are capable of regulating the development of many organs and tissues. We previously identifiedDkk3to be a molecule predominantly expressed in the mouse embryonic retina. However, which cell expressesDkk3in the developing and mature mouse retina remains to be elucidated. To examine the precise expression of theDkk3protein, we generatedBAC-Dkk3-EGFPtransgenic mice that express EGFP integrated into theDkk3gene in a BAC plasmid. Expression analysis using theBAC-Dkk3-EGFPtransgenic mice revealed thatDkk3is expressed in retinal progenitor cells (RPCs) at embryonic stages and in Müller glial cells in the adult retina. Since Müller glial cells may play a potential role in retinal regeneration,BAC-Dkk3-EGFPmice could be useful for retinal regeneration studies.

scholarly journals Fibroblast Growth Factor (FGF) Soluble Receptor 1 Acts as a Natural Inhibitor of FGF2 Neurotrophic Activity during Retinal Degeneration

1998 ◽  
Vol 9 (10) ◽  
pp. 2785-2802 ◽  
Author(s):  
Xavier Guillonneau ◽  
Fabienne Régnier-Ricard ◽  
Olivier Laplace ◽  
Laurent Jonet ◽  
Marijke Bryckaert ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Glial Cells ◽  
Tyrosine Kinase Receptor ◽  
Mrna Levels ◽  
Fibroblast Growth ◽  
Adult Retina ◽  
Müller Glial Cells ◽  
Neurotrophic Activity ◽  
Natural Inhibitor

Fibroblast growth factors (FGF) 1 and 2 and their tyrosine kinase receptor (FGFR) are present throughout the adult retina. FGFs are potential mitogens, but adult retinal cells are maintained in a nonproliferative state unless the retina is damaged. Our work aims to find a modulator of FGF signaling in normal and pathological retina. We identified and sequenced a truncated FGFR1 form from rat retina generated by the use of selective polyadenylation sites. This 70-kDa form of soluble extracellular FGFR1 (SR1) was distributed mainly localized in the inner nuclear layer of the retina, whereas the full-length FGFR1 form was detected in the retinal Muller glial cells. FGF2 and FGFR1 mRNA levels greatly increased in light-induced retinal degeneration. FGFR1 was detected in the radial fibers of activated retinal Muller glial cells. In contrast, SR1 mRNA synthesis followed a biphasic pattern of down- and up-regulation, and anti-SR1 staining was intense in retinal pigmented epithelial cells. The synthesis of SR1 and FGFR1 specifically and independently regulated in normal and degenerating retina suggests that changes in the proportion of various FGFR forms may control the bioavailability of FGFs and thus their potential as neurotrophic factors. This was demonstrated in vivo during retinal degeneration when recombinant SR1 inhibited the neurotrophic activity of exogenous FGF2 and increased damaging effects of light by inhibiting endogenous FGF. This study highlights the significance of the generation of SR1 in normal and pathological conditions.

What do retinal Müller (glial) cells do for their neuronal ‘small siblings’?

1993 ◽  
Vol 6 (4) ◽  
pp. 201-213 ◽  
Author(s):  
A. Reichenbach ◽  
J.-U. Stolzenburg ◽  
W. Eberhardt ◽  
T.I. Chao ◽  
D. Dettmer ◽  
...  
Keyword(s):  
Glial Cells ◽  
Müller Glial Cells

GABAA receptor currents recorded from Müller glial cells of the baboon (Papio cynocephalus) retina

1996 ◽  
Vol 203 (3) ◽  
pp. 159-162 ◽  
Author(s):  
Winfried Reichelt ◽  
Miguel Hernandez ◽  
Raymond T. Damian ◽  
William S. Kisaalita ◽  
Berry L. Jordan
Keyword(s):  
Gabaa Receptor ◽  
Glial Cells ◽  
Papio Cynocephalus ◽  
Müller Glial Cells

Blood-derived macrophages infiltrate the retina and activate Muller glial cells under experimental choroidal neovascularization

2005 ◽  
Vol 81 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Alejandro Caicedo ◽  
Diego G. Espinosa-Heidmann ◽  
Yolanda Piña ◽  
Eleut P. Hernandez ◽  
Scott W. Cousins
Keyword(s):  
Choroidal Neovascularization ◽  
Glial Cells ◽  
Müller Glial Cells

The glutathione content of retinal Müller (glial) cells: effect of pathological conditions

2000 ◽  
Vol 36 (4-5) ◽  
pp. 461-469 ◽  
Author(s):  
Dominik Huster ◽  
Andreas Reichenbach ◽  
Winfried Reichelt
Keyword(s):  
Glial Cells ◽  
Müller Glial Cells ◽  
Pathological Conditions

Voltage- and GABA-evoked currents from Müller glial cells of the baboon retina

Neuroreport ◽  
1997 ◽  
Vol 8 (2) ◽  
pp. 541-544 ◽  
Author(s):  
Winfried Reichelt ◽  
Miguel Hernandez ◽  
Raymond T. Damian ◽  
William S. Kisaalita ◽  
Berry L. Jordan
Keyword(s):  
Glial Cells ◽  
Müller Glial Cells
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