scholarly journals Efficient Production Process for Food Grade Acetic Acid byAcetobacter acetiin Shake Flask and in Bioreactor Cultures

2012 ◽  
Vol 9 (4) ◽  
pp. 2275-2286 ◽  
Author(s):  
Hassan M. Awad ◽  
Richard Diaz ◽  
Roslinda A. Malek ◽  
Nor Zalina Othman ◽  
Ramlan A. Aziz ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Production Process ◽  
Yeast Extract ◽  
Acid Production ◽  
Shake Flask ◽  
Efficient Production ◽  
Acetobacter Aceti ◽  
Stirred Tank Bioreactor ◽  
Acetic Acid Production ◽  
Medium Components

Acetic acid is one of the important weak acids which had long history in chemical industries. This weak organic acid has been widely used as one of the key intermediate for many chemical, detergent, wood and food industries. The production of this acid is mainly carried out using submerged fermentation system and the standard strainAcetobacter aceti. In the present work, six different media were chosen from the literatures and tested for acetic acid production. The highest acetic acid production was produced in medium composed of glucose, yeast extract and peptone. The composition of this medium was optimized by changing the concentration of medium components. The optimized medium was composed of (g/L): glucose, 100; yeast extract, 12 and peptone 5 and yielded 53 g/L acetic acid in shake flask after 144 h fermentation. Further optimization in the production process was achieved by transferring the process to semi-industrial scale 16-L stirred tank bioreactor and cultivation under controlled pH condition. Under fully aerobic conditions, the production of acetic acid reached maximal concentration of about 76 g/L and 51 g/L for uncontrolled and controlled pH cultures, respectively.

Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti

2013 ◽  
Vol 115 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Kenta Sakurai ◽  
Shoko Yamazaki ◽  
Masaharu Ishii ◽  
Yasuo Igarashi ◽  
Hiroyuki Arai
Keyword(s):  
Acetic Acid ◽  
Acid Production ◽  
Acetobacter Aceti ◽  
Acetic Acid Production ◽  
Glyoxylate Pathway

scholarly journals Development of Acetic Acid Production Process Catalyzed by Rhodium Complex Immobilized to Pyridine Polymer Support

2003 ◽  
Vol 46 (4) ◽  
pp. 240-248 ◽  
Author(s):  
Noriyuki YONEDA ◽  
Takeshi MINAMI ◽  
Yoshimi SHIROTO ◽  
Makoto YASUI ◽  
Tadashi MATSUMOTO ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Production Process ◽  
Acid Production ◽  
Rhodium Complex ◽  
Polymer Support ◽  
Acetic Acid Production

Acetic acid and hydrogen metabolism during coculture of an acetic acid producing bacterium with methanogenic bacteria

1978 ◽  
Vol 24 (8) ◽  
pp. 1007-1010 ◽  
Author(s):  
G. B. Patel ◽  
L. A. Roth
Keyword(s):  
Acetic Acid ◽  
Gas Phase ◽  
Yeast Extract ◽  
Acid Production ◽  
Methanogenic Bacteria ◽  
The Other ◽  
Hydrogen Metabolism ◽  
Acetic Acid Production ◽  
Gram Negative ◽  
Acetate Formation

Two microorganisms originally existing as a mixed culture obtained from an anaerobic digester fluid were separated for pure and coculture studies. One of these was motile, Gram-negative, and non-sporeforming, and it required yeast extract for growth and acetic acid production. This isolate produced H2 and did not need H2 and (or) CO2 for growth and acetate formation. The other isolate was a methanogen which resembled Methanobacterium arbophilicum in morphology and substrate specificity. Coculture growth of the two isolates in yeast extract broth (80% N2–20% CO2 gas phase) indicated that the non-methanogen produced up to four to five times more H2 than when grown separately. Although the growth of the non-methanogen was not enhanced by the removal of H2 by the methanogen, the hydrogen produced was essential for the growth of methanogen. Similar results were obtained when the non-methanogen was cocultured with Methanospirillum hungatii GP1. Cultivation of the non-methanogen in the presence of M. hungatii GP1 (under abundance of 80% H2–20% CO2) indicated that the acetate produced was consumed by M. hungatii, without inhibiting the growth of the other culture.

Enhancement of acetic acid production in a high cell-density culture of Acetobacter aceti

1989 ◽  
Vol 68 (5) ◽  
pp. 315-319 ◽  
Author(s):  
Yong Soo Park ◽  
Hisao Ohtake ◽  
Masahiro Fukaya ◽  
Hajime Okumura ◽  
Yoshiya Kawamura ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Density ◽  
Acid Production ◽  
High Cell Density ◽  
High Cell Density Culture ◽  
High Cell ◽  
Acetobacter Aceti ◽  
Acetic Acid Production
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