scholarly journals A Study of the Granulomatous Responses Induced by Different Strains ofSchistosoma mansoni

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nádia Regina Borim Zuim ◽  
Silmara Marques Allegretti ◽  
Arício Xavier Linhares ◽  
Luiz Augusto Magalhães ◽  
Eliana Maria Zanotti-Magalhães
Keyword(s):  
Schistosoma Mansoni ◽  
The Other ◽  
Host Reaction ◽  
Schistosome Infection ◽  
Experimental Infections ◽  
Tissue Area ◽  
Caudal Vein ◽  
Different Strains

The increased pathogenesis of theSchistosoma mansoniBH strain compared with the SJ strain has been attributed to the number of granulomas formed in experimental infections, which increase the mortality in definitive hosts. The aim of the present study was to investigate the development of granulomas around the eggs of theS. mansoniBH and SJ strains and to determine whether this host reaction was strain specific. Four experimental groups were analyzed. Two groups contained mice inoculated in the caudal vein with eggs from theS. mansoniBH or SJ strains and the other two contained mice that were infected with cercariae of the BH strain prior to being inoculated with eggs. The number of granulomas per tissue area in the lungs and liver, as well as the size of the granulomas, was analyzed to characterize the response to schistosome infection. The largest granulomatous responses were observed around eggs of the BH strain. Granulomas covered a larger area in the lungs of mice that were previously infected with cercariae and subsequently inoculated with eggs of the BH strain. These results indicated that specific granulomatous responses occurred following an infection with the BH and SJ strains ofS. mansoni.

scholarly journals Experimental Evaluation of the Pathogenicity of Different Strains ofSchistosoma mansoni

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Antônio Aurélio Euzébio ◽  
Nádia Regina Borim Zuim ◽  
Arício Xavier Linhares ◽  
Luiz Augusto Magalhães ◽  
Eliana Maria Zanotti-Magalhães
Keyword(s):  
Schistosoma Mansoni ◽  
Experimental Evaluation ◽  
Biomphalaria Glabrata ◽  
Sao Paulo ◽  
São Paulo ◽  
Ascending Colon ◽  
Worm Recovery ◽  
Tissue Area ◽  
Different Strains ◽  
The City

The pathogenesis of three differentSchistosoma mansonistrains from the Brazilian states of Minas Gerais (BH strain) and São Paulo (SJ and SD strains) was evaluated in experimentally infected mice. Observations of the most severe clinical cases among local patients treated (SD strain) in the city of Campinas (São Paulo, Brazil) formed the basis of this study. Mice were used as definitive hosts and were infected with cercariae fromBiomphalaria tenagophila(SJ and SD strains) andBiomphalaria glabrata(BH strains). The parameters analyzed were as follows: number ofS. mansonieggs in mice feces; number of granulomas per tissue area in liver, spleen, lungs, pancreas, and ascending colon; measurements of hepatic and intestinal granulomas; number of adult worms; and measurements of trematode eggs. The comparison among the three strains indicated that the SD strain, isolated in Campinas, presented a higher worm recovery relative to the number of penetrating cercariae. In addition, when compared to the SJ and BH strains, the SD strain demonstrated similar pathogenicity to the BH strain, with a greater quantity of granulomas in the viscera, as well as larger granulomas and eggs. Furthermore, a greater quantity of trematode eggs was also shed in the feces.

OBSERVATIONS ON EXPERIMENTAL INFECTIONS WITH ECHINOCOCCUS IN RODENTS

1961 ◽  
Vol 39 (6) ◽  
pp. 877-891 ◽  
Author(s):  
Gloria A. Webster ◽  
T. W. M. Cameron
Keyword(s):  
Echinococcus Granulosus ◽  
Echinococcus Multilocularis ◽  
Early Stage ◽  
The Other ◽  
Host Reaction ◽  
Microscopic Anatomy ◽  
Larval Parasite ◽  
Experimental Infections ◽  
White Rats ◽  
Cotton Rats

Experimental infections in rodents with Echinococcus multilocularis sibiricensis, Echinococcus granulosus canadensis, and Echinococcus granulosus (Lebanon origin) both by ingestion of onchospheres and by parenteral injection are recorded. The microscopic anatomy of the larval parasite and of the host reaction are discussed and illustrated in detail. Voles and cotton rats display little resistance to the development of the cyst of E. m. sibiricensis and ultimately destroy the host, while collared lemmings and golden hamsters overcome the cyst and destroy it. Muskrats and certain white mice are intermediate in their effects. In all cases, however, some degree of degeneration of the parasite into the alveolar form is shown. White rats, guinea pigs, and rabbits could not be infected with this parasite. E. g. canadensis was developing in the lung of one AKR strain mouse while in the chinchilla the parasite commenced to develop in the lung but was overcome at an early stage. No development took place in the other species of hosts. E. granulosus (Lebanon) appeared to be developing successfully in some cotton rats and white mice fed on onchospheres.

Susceptibilities of Various Snail Intermediate Hosts of Schistosoma mansoni to Different Strains of the Parasite

1965 ◽  
Vol 39 (4) ◽  
pp. 363-376 ◽  
Author(s):  
M.F.A. Saoud
Keyword(s):  
Puerto Rico ◽  
Schistosoma Mansoni ◽  
Intermediate Hosts ◽  
Considerable Evidence ◽  
The Past ◽  
Host Parasite Relationship ◽  
Parasite Relationship ◽  
Different Strains ◽  
Host Parasite ◽  
Comprehensive Studies

In the past two decades, considerable evidence has accumulated in the literature about the differences in the susceptibility of various intermediate hosts of Schistosoma mansoni to different strains of the parasite. Comprehensive studies on this aspect of host-parasite relationship have been published by Files & Cram (1949), Abdel-Malek (1950) and Files (1951). The results of more recent studies have been reported by Wright (1962) and Saoud (1964).In the present paper, the writer has studied the susceptibility of four intermediate hosts of S. mansoni from Brazil, Puerto Rico, Egypt and Tanganyika to some strains of the parasite.

Effects of three azole derivatives on the lipids of different strains ofSporothrix schenckii

1997 ◽  
Vol 43 (12) ◽  
pp. 1197-1202 ◽  
Author(s):  
Agnes Kiesling Casali ◽  
Júnia Soares Hamdan
Keyword(s):  
Inhibitory Concentration ◽  
Sporothrix Schenckii ◽  
The Other ◽  
Ergosterol Biosynthesis ◽  
Complete Agreement ◽  
Sterol Content ◽  
Ultraviolet Spectrophotometry ◽  
Different Strains ◽  
Layer Chromatography ◽  
Azole Derivative

The comparative effects of ketoconazole, itraconazole, and fluconazole on the lipids of five Sporothrix schenckii strains were investigated. Quantitative analysis of lipids and sterols was completed, as well as qualitative analysis of sterols, by thin-layer chromatography and ultraviolet spectrophotometry. Growth of the S. schenckii isolates in the presence of azole derivative concentrations below the minimum inhibitory concentration (MIC) resulted in significant alterations in the lipid and sterol contents as compared with the control values. Furthermore, lanosterol was detected in these azole-treated cells. These results were in complete agreement with the proposed mechanism of action of azoles, which act by inhibiting ergosterol biosynthesis with a consequent accumulation of lanosterol. Concerning the MIC values, fluconazole was found to be the least effective drug. On the other hand, as determined from a comparison of the effects of the three azoles on the sterol content of the strains studied, no significant differences in efficacy were found among the tested drugs.Key words: Sporothrix schenckii, azole derivatives, lipids, sterols.

Hybrids between Schistosoma mansoni and S. rodhaini: characterization by cercarial emergence rhythms

Parasitology ◽  
1989 ◽  
Vol 99 (2) ◽  
pp. 225-228 ◽  
Author(s):  
A. Thèron
Keyword(s):  
Schistosoma Mansoni ◽  
Parental Species ◽  
Genetic Determinism ◽  
The Other ◽  
Relative Importance ◽  
Emergence Rhythm ◽  
Cercarial Emergence

SummaryHybridization between Schistosoma mansoni, with a diurnal cercarial emergence rhythm and S. rodhaini, with a nocturnal cercarial shedding pattern leads to F1 and F2 generations, hybrid schistosomes whose chronobiological phenotype of cercariae is characterized by two unequal emergence peaks, one diurnal and the other nocturnal. The relative importance of diurnal and nocturnal peaks depends upon which S. mansoni strain (early or late) is used for the hybridization with S. rodhaini. The results are compared and discussed with those resulting from crosses between intraspecific sympatric and allopatric chronobiological variants (early and late) of S. mansoni. The genetic determinism of the cercarial emergence of schistosomes and the significant differences observed between cercarial shedding patterns of parental species and their hybrids allow the use of this behavioural marker in biological and genetical studies in schistosome populations.

scholarly journals Brief Report: Graft-Versus-Host Reaction in F1 Hybrid Mice Injected with Pre-Immunized Parental Thymus Cells

Blood ◽  
1964 ◽  
Vol 24 (6) ◽  
pp. 770-774 ◽  
Author(s):  
LUCIANO FIORE-DONATI ◽  
LUIGI CHIECO-BIANCHI ◽  
GIUSEPPE DE BENEDICTIS ◽  
GIUSEPPE TRIDENTE
Keyword(s):  
Lymphoid Cells ◽  
The Other ◽  
F1 Hybrids ◽  
Graft Versus Host Reaction ◽  
Host Reaction ◽  
F1 Hybrid ◽  
Graft Versus Host ◽  
Immunologic Activity ◽  
Thymus Cells ◽  
Hybrid Mice

Abstract Dissociated thymus cells are capable of initiating graft-versus-host reaction in (C3Hf/Gs x DBA/2)F1 hybrids only when derived from parental donors previously sensitized against the antigens of the other parental strain. The lower immunologic activity of thymus cells as compared with other lymphoid cells is presumably due to quantitative rather than qualitative differences in immunologically competent cells.

scholarly journals Effect of wide spectrum anti-helminthic drugs upon Schistosoma mansoni experimentally infected mice

1997 ◽  
Vol 39 (3) ◽  
pp. 159-164 ◽  
Author(s):  
Christiane Finardi PANCERA ◽  
Adriana Leal ALVES ◽  
Maria Aparecida PASCHOALOTTI ◽  
Pedro Paulo CHIEFFI
Keyword(s):  
Schistosoma Mansoni ◽  
Wide Spectrum ◽  
Nematode Species ◽  
The Other ◽  
Fecal Shedding ◽  
The Third ◽  
Other Hand ◽  
Evolution Period

Mebendazole, albendazole, levamisole and thiabendazole are well known as active drugs against several nematode species, and against cestodes as well, when the first two drugs are considered. None of the drugs have proven activity, however, against trematodes. We tested the effect of these drugs on the fecal shedding of schistosome eggs and the recovering of adult schistosomes, after portal perfusion in Schistosoma mansoni experimentally infected mice. Balb/c mice infected with 80 S. mansoni cercariae were divided into three groups, each in turn subdivided into four other groups, for each tested drug. The first group was treated with each one of the studied drugs 25 days after S. mansoni infection; the second group was submitted to treatment with each one of the drugs 60 days after infection. Finally, the third group, considered as control, received no treatment. No effect upon fecal shedding of S. mansoni eggs and recovering of schistosomes after portal perfusion was observed when mice were treated with either mebendazole or albendazole. Mice treated with either levamisole or thiabendazole, on the other hand, showed a significant reduction in the recovering of adult schistosomes after portal perfusion, mainly when both drugs were given during the schistosomula evolution period, i.e., 25 days after cercariae penetration, probably due to unspecific immunomodulation

The Effect of Presurgical Treatment on Palatal Tissue Area in Unilateral Cleft Lip and Palate Subjects

1977 ◽  
Vol 4 (4) ◽  
pp. 181-185 ◽  
Author(s):  
A. G. Huddart ◽  
J. J. Crabb
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
The Other ◽  
Normal Children ◽  
Orthopaedic Treatment ◽  
Tissue Area

The investigation measures the area of palatal tissue at birth and at four months in three groups of subjects: (1) 30 complete unilateral cleft lip and palate cases who received presurgical maxillary orthopaedic treatment; (2) 15 similar cases who did not receive such treatment and were used as controls; (3) 30 normal children with intact palates. The changes occurring in the three groups of cases over the four month period are compared. In particular it was noted that presurgical treatment retarded the growth of palatal tissue. The significance of this in relation to the other findings and presurgical treatment generally is discussed.

scholarly journals Mass Spectrometry-Based Characterization of New Spirolides from Alexandrium ostenfeldii (Dinophyceae)

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 505
Author(s):  
Joyce A. Nieva ◽  
Jan Tebben ◽  
Urban Tillmann ◽  
Sylke Wohlrab ◽  
Bernd Krock
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
Molecular Ions ◽  
Ring System ◽  
The Other ◽  
Spectral Techniques ◽  
Mass Spectral ◽  
Alexandrium Ostenfeldii ◽  
Different Strains

Spirolides belong to a group of marine phycotoxins produced by the marine planktonic dinophyte Alexandrium ostenfeldii. Composed of an imine moiety and a spiroketal ring system within a macrocylcle, spirolides are highly diverse with toxin types that vary among different strains. This study aims to characterize the spirolides from clonal A. ostenfeldii strains collected from The Netherlands, Greenland and Norway by mass spectral techniques. The structural characterization of unknown spirolides as inferred from high-resolution mass spectrometry (HR-MS) and collision induced dissociation (CID) spectra revealed the presence of nine novel spirolides that have the pseudo-molecular ions m/z 670 (1), m/z 666 (2), m/z 696 (3), m/z 678 (4), m/z 694 (5), m/z 708 (6), m/z 720 (7), m/z 722 (8) and m/z 738 (9). Of the nine new spirolides proposed in this study, compound 1 was suggested to have a truncated side chain in lieu of the commonly observed butenolide ring in spirolides. Moreover, there is indication that compound 5 might belong to new spirolide subclasses with a trispiroketal ring configuration having a 6:5:6 trispiroketal ring system. On the other hand, the other compounds were proposed as C- and G-type SPX, respectively. Compound 7 is proposed as the first G-type SPX with a 10-hydroxylation as usually observed in C-type SPX. This mass spectrometry-based study thus demonstrates that structural variability of spirolides is larger than previously known and does not only include the presence or absence of certain functional groups but also involves the triketal ring system.

scholarly journals Egg excretion in the initial phase of experimental murine schistosomiasis mansoni: stability and association with worm burden

1995 ◽  
Vol 37 (4) ◽  
pp. 325-329 ◽  
Author(s):  
Regina Lunardi Rocha ◽  
Manoel Otávio da Costa Rocha ◽  
Ênio Roberto Pietra Pedroso ◽  
Enrico Antônio Colosimo ◽  
Paulo Marcos Zech Coelho
Keyword(s):  
Schistosoma Mansoni ◽  
Initial Phase ◽  
Worm Burden ◽  
Chronic Phase ◽  
Infection Intensity ◽  
Schistosome Infection ◽  
Schistosomiasis Mansoni

Stability of faecal egg excretion and correlation with results related to worm burden at the initial phase of schistosomiasis mansoni were observed in two groups of mice infected with different Schistosoma mansoni cercarial burdens, by means of analysis of quantitative parasitological studies and schistosome counts after perfusion. Thus, it may be stated that few quantitative parasitological stool examinations could be sufficient to express the infection intensity at the initial phase, on the same grounds that it was already demonstrated at the chronic phase. Furthermore, it is confirmed that the use of the number of eggs passed in the faeces as a tool to estimate the worm burden at the initial phase of schistosome infection is adequate.

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