scholarly journals Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Biswanath Bhunia ◽  
Apurba Dey
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Tween 80 ◽  
Culture Conditions ◽  
Protease Production ◽  
Sodium Perborate ◽  
Physiochemical Parameters ◽  
Peg 4000 ◽  
Detergent Stability ◽  
Triton X

The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84 min with 73.5% purity. This enzyme retained more than 92% of its initial activity after preincubation for 30 min at 37∘C in the presence of 25% v/v DMSO, methanol, ethanol, ACN, 2-propanol, benzene, toluene, and hexane. In addition, partially purified enzyme showed remarkable stability for 60 min at room temperature, in the presence of anionic detergent (Tween-80 and Triton X-100), surfactant (SDS), bleaching agent (sodium perborate and hydrogen peroxide), and anti-redeposition agents (Na2CMC, Na2CO3). Purified enzyme containing 10% w/v PEG 4000 showed better thermal, surfactant, and local detergent stability.

scholarly journals A Novel Thermostable and Alkaline Protease Produced from Bacillus stearothermophilus Isolated from Olive Oil Mill Sols Suitable to Industrial Biotechnology

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1139
Author(s):  
Aida Karray ◽  
Mona Alonazi ◽  
Habib Horchani ◽  
Abir Ben Bacha
Keyword(s):  
Cell Growth ◽  
Olive Oil ◽  
Incubation Time ◽  
Alkaline Protease ◽  
Bacillus Stearothermophilus ◽  
Nitrogen Sources ◽  
Reversed Phase ◽  
Culture Conditions ◽  
Protease Production ◽  
Industrial Biotechnology

This study was conducted to identify a new alkaline and thermophilic protease (Ba.St.Pr) produced from Bacillus stearothermophilus isolated from olive oil mill sols and to evaluate its culture conditions, including temperature, pH, carbon and nitrogen sources, and incubation time. The optimum culture conditions for cell growth (10 g/L) and protease production (5050 U/mL) were as follows: temperature 55 °C, pH 10, inoculation density 8 × 108 CFU/mL, and incubation time 24 h. The use of 3% yeast extract as the nitrogen sources and galactose (7.5 g/L) as the carbon sources enhanced both cell growth and protease production. Using reversed-phase analytical HPLC on C-8 column, the new protease was purified with a molecular mass of approximately 28 kDa. The N-terminal sequence of Ba.St.Pr exhibited a high level of identity of approximately 95% with those of Bacillus strains. Characterization under extreme conditions revealed a novel thermostable and alkaline protease with a half-life time of 187 min when incubated with combined Ca2+/mannitol. Ba.St.Pr demonstrated a higher stability in the presence of surfactant, solvent, and Ca2+ ions. Consequently, all the evaluated activity parameters highlighted the promising properties of this bacterium for industrial and biotechnological applications.

scholarly journals Optimization of Nutrients and Culture Conditions for Alkaline Protease Production Using Two Endophytic Micrococci: Micrococcus aloeverae and Micrococcus yunnanensis

2017 ◽  
Vol 57 (2) ◽  
pp. 218-225 ◽  
Author(s):  
Om Prakash ◽  
Yogesh Nimonkar ◽  
Mahesh S. Chavadar ◽  
Nidhi Bharti ◽  
Shrikant Pawar ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Culture Conditions ◽  
Protease Production

scholarly journals Characterization and Optimization of Alkaline Protease Production from Bacillus licheniformis HSW-16 Isolated from Sambhar Salt Lake

2015 ◽  
Vol 3 (2) ◽  
pp. 347-351
Author(s):  
Rajnish Prakash Singh ◽  
Prabhat Nath Jha
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Salt Lake ◽  
Industrial Applications ◽  
Protease Production ◽  
Protease Gene ◽  
Potential Source ◽  
Alkaline Protease Gene ◽  
Bacterium Bacillus ◽  
Ph Range

Halophilic microorganisms are recognized as potential source of secondary metabolites including enzymes and drugs with wide agricultural and industrial applications. In the present study protease producing halotolerant bacterium Bacillus licheniformis HSW-16 was isolated from hypersaline Sambhar lake, Rajasthan India. Protease production was performed by using azocasein as substrate. Confirmation of protease production was also done by amplification of alkaline protease gene and sequencing. The various nutritional factors such as carbon and nitrogen source and other physiological parameters like pH, temperature, incubation time and agitation speed were optimized for optimum protease production. The enzyme was active in pH range 7-10, temperature 25 °C-40 °C and salt concentration of 1.5M. The characteristics demonstrated by this isolate showed that it could be used as a potential source of enzyme.Int J Appl Sci Biotechnol, Vol 3(2): 347-351 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12757 

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