Regulatory effects of alanine-group amino acids on serine alkaline protease production by recombinant Bacillus licheniformis

2003 ◽  
Vol 37 (2) ◽  
pp. 165 ◽  
Author(s):  
Pınar alık ◽  
Arzu Bayram ◽  
Tuner H. zdamar
Keyword(s):  
Amino Acids ◽  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Protease Production ◽  
Serine Alkaline Protease ◽  
Regulatory Effects

Metabolic flux analyses for serine alkaline protease production

2000 ◽  
Vol 27 (10) ◽  
pp. 793-805 ◽  
Author(s):  
Pınar Çalik ◽  
Güzide Çalik ◽  
Serpil Takaç ◽  
Tunçer H Özdamar
Keyword(s):  
Alkaline Protease ◽  
Metabolic Flux ◽  
Protease Production ◽  
Serine Alkaline Protease

scholarly journals Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Biswanath Bhunia ◽  
Apurba Dey
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Tween 80 ◽  
Culture Conditions ◽  
Protease Production ◽  
Sodium Perborate ◽  
Physiochemical Parameters ◽  
Peg 4000 ◽  
Detergent Stability ◽  
Triton X

The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84 min with 73.5% purity. This enzyme retained more than 92% of its initial activity after preincubation for 30 min at 37∘C in the presence of 25% v/v DMSO, methanol, ethanol, ACN, 2-propanol, benzene, toluene, and hexane. In addition, partially purified enzyme showed remarkable stability for 60 min at room temperature, in the presence of anionic detergent (Tween-80 and Triton X-100), surfactant (SDS), bleaching agent (sodium perborate and hydrogen peroxide), and anti-redeposition agents (Na2CMC, Na2CO3). Purified enzyme containing 10% w/v PEG 4000 showed better thermal, surfactant, and local detergent stability.

Protein-based complex medium design for recombinant serine alkaline protease production

2003 ◽  
Vol 33 (7) ◽  
pp. 975-986 ◽  
Author(s):  
Pınar Çalık ◽  
Eda Çelik ◽  
İlkin E Telli ◽  
Ceren Oktar ◽  
Elif Özdemir
Keyword(s):  
Alkaline Protease ◽  
Complex Medium ◽  
Protease Production ◽  
Serine Alkaline Protease ◽  
Medium Design

Serine alkaline protease overproduction capacity of Bacillus licheniformis

2000 ◽  
Vol 26 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Pınar Çalık ◽  
Serpil Takaç ◽  
Güzide Çalık ◽  
Tunçer H Özdamar
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Serine Alkaline Protease
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