scholarly journals Relationship between Plasma Ferritin Level and Siderocyte Number in Splenectomizedβ-Thalassemia/HbE Patients

Anemia ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
A. Tripatara ◽  
N. Srichana ◽  
P. Lamool ◽  
S. Amnuaykan ◽  
P. Hongart ◽  
...  
Keyword(s):  
Iron Overload ◽  
Ferritin Level ◽  
Receiver Operating Curve ◽  
Blue Staining ◽  
Excess Iron ◽  
Receiver Operating Curve Analysis ◽  
Normal Individuals ◽  
Iron Deposits ◽  
Complete Blood Counts ◽  
The Relationship

Introduction. In iron overload status, excess iron deposits in reticuloendothelial cells and tissues and can be detected using Prussian blue staining. The aim of this paper was to investigate the relationship between siderocyte numbers and plasma ferritin levels (a practically standard marker of iron overload) in the blood of the splenectomized and nonsplenectomizedβ-thalassemia/HbE patients, who are at risk of iron overload.Methods. EDTA blood samples from 64 patients with 35 splenectomized and 29 nonsplenectomizedβ-thalassemia/HbE patients, who received regular blood transfusions, and 20 normal individuals were investigated for siderocyte numbers, plasma ferritin levels, and complete blood counts.Results. The average percent siderocytes in splenectomized and nonsplenectomizedβ-thalassemia/HbE patients were 11.5% and 0.08%, respectively, and plasma ferritin levels of 2,332 μg/L and 1,279 μg/L, respectively. Percent siderocytes showed a good correlation with plasma ferritin levels only in splenectomized patients (r=0.69,P<0.001). A receiver operating curve analysis from splenectomized patients’ data indicated that siderocytes at 3% cut-off are the best predictor for plasma ferritin level ≥1,000 μg/L with 92.9% sensitivity and 42.9% specificity.Conclusion. Circulating siderocyte numbers can be used as a screening test for the assessment of the iron overload in splenectomizedβ-thalassemia/HbE patients in the place where serum ferritin is not available.

A Method for Rapid Purification of Murine Siderocytes.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3703-3703
Author(s):  
Florent M. Martin ◽  
Gabriela Bydlon ◽  
Megan L. Welsh ◽  
Jeffrey S. Friedman
Keyword(s):  
Iron Overload ◽  
Protein Composition ◽  
Specific Method ◽  
Iron Compounds ◽  
Hematopoietic Stem ◽  
Excess Iron ◽  
Human Disorders ◽  
Iron Deposits ◽  
Rapid Purification ◽  
Separation Columns

Abstract Iron overload is a key contributor to the pathogenesis of multiple human disorders including the sideroblastic anemias. The specific iron compounds present in tissues or cells that are the targets of excess iron deposition remain poorly understood. Our objective is to further identify the composition of such iron aggregates. As there is evidence that some forms are magnetically active, we have developed a simple and specific method to purify iron-overloaded red blood cells using magnetic affinity columns. RBC derived from mice transplanted with SOD2-deficient hematopoietic stem cells served as a source of iron-laden cells (Friedman et al., Blood 2004; J. Exp. Med. 2001). Purification was based upon the observation that iron deposits in SOD2-deficient cells are magnetically susceptible and allow for retention of iron-laden cells in a strong magnetic field. Peripheral blood from SOD2-deficient chimeric mice was passed through magnetic separation columns; iron-overloaded cells were eluted and subsequently characterized by flow cytometry, western blot and microscopy. We were able to purify 3% of the total red cells as iron-laden siderocytes. More than 93% of the magnetically purified SOD2-deficient cells were identified as reticulocytes; they had numerous siderotic granules, produced enhanced reactive oxygen species levels, and showed increased protein oxidative damage, mitochondrial enrichment and mitochondrial membrane potential. Our method can be used to purify iron-laden cells as well as iron-associated subcellular fractions prepared from iron-loaded tissues, allowing elucidation of the structure, location and protein composition of such iron deposits. We believe that this data will help develop our understanding of the pathogenesis of sideroblastic anemia and other disorders characterized by iron overload.

scholarly journals Evaluation of iron overload by cardiac and liver T2* in β-thalassemia: Correlation with serum ferritin, heart function and liver enzymes

2021 ◽  
Vol 13 (1) ◽  
pp. 54-60
Author(s):  
Hengameh Khadivi Heris ◽  
Babak Nejati ◽  
Khatereh Rezazadeh ◽  
Hossein Sate ◽  
Roya Dolatkhah ◽  
...  
Keyword(s):  
Iron Overload ◽  
Serum Ferritin ◽  
Serum Ferritin Level ◽  
Liver Enzymes ◽  
Heart Function ◽  
Ferritin Level ◽  
Diagnostic Methods ◽  
Iron Load ◽  
The Relationship ◽  
T2 Mri

Introduction: In this study, we aimed to assess the relationship of cardiac and hepatic T2* magnetic resonance imaging (MRI) values as a gold standard for detecting iron overload with serum ferritin level, heart function, and liver enzymes as alternative diagnostic methods. Methods: A total 58 patients with beta-thalassemia major who were all transfusion dependent were evaluated for the study. T2* MRI of heart and liver, echocardiography, serum ferritin level, and liver enzymes measurement were performed. The relationship between T2* MRI findings and other assessments were examined. Cardiac and hepatic T2* findings were categorized as normal, mild, moderate, and severe iron overload. Results: 22% and 11% of the patients were suffering from severe iron overload in heart and liver, respectively. The echocardiographic findings were not significantly different among different iron load categories in heart or liver. ALT level was significantly higher in patient with severe iron overload than those with normal iron load in heart (P=0.005). Also, AST level was significantly lower in normal iron load group than mild, moderate, and severe iron load groups in liver (P<0.05). The serum ferritin level was significantly inversely correlated with cardiac T2* values (r = -0.34, P=0.035) and hepatic T2* values (r = -0.52, P=0.001). Conclusion: Cardiac and hepatic T2* MRI indicated significant correlation with serum ferritin level.

Plasma Level of IL-1β in Disseminated Intravascular Goagulation

1991 ◽  
Vol 65 (04) ◽  
pp. 364-368 ◽  
Author(s):  
Hideo Wada ◽  
Shigehisa Tamaki ◽  
Motoaki Tanigawa ◽  
Mikio Takagi ◽  
Yoshitaka Mori ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tumor Necrosis Factor ◽  
Plasma Level ◽  
Organ Failure ◽  
Tumor Necrosis ◽  
Mononuclear Cells ◽  
Solid Cancer ◽  
Normal Individuals ◽  
The Relationship ◽  
Necrosis Factor

SummaryThe plasma level of interleukin-1β (IL-1β) was determined in normal individuals, patients with disseminated intravascular coagulation (DIC), patients in the pre-DIC period (within 7 days before the onset of DIC), and non-DIC patients to examine the relationship between DIC and the plasma ILlp level. The plasma IL-1β level was 0-0.085 ng/ml in normal individuals, with little difference being seen according to related age. It was significantly higher in the DIC group (0.19 ± 0.19 ng/ml) than in the pre-DIC group (0.05 ± 0.08 ng/ml) or the non-DIC group (0.09 ± 0.01 ng/ml). The plasma IL-1β level was not markedly elevated in leukemia patients, even in the DIC group, but it was significantly increased in the DIC group of solid cancer patients and was generally elevated in patients with sepsis. It was markedly elevated to 0.39 ± 0.26 ng/ml in patients with organ failure. When mononuclear cells were incubated with lipopolysaccharide, it was found that IL-1β, tumor necrosis factor, and tissue factor (TF) were released into the medium, and there was an increase of TF release from endothelial cells incubated with this medium. These results suggest that the increase in IL-Iβ reflected the activation of monocytes and may be an important factor in DIC and its associated organ failure.

scholarly journals Assessing early erythrolysis and the relationship to perihematomal iron overload and white matter survival in human intracerebral hemorrhage

2021 ◽  
Author(s):  
Nemanja Novakovic ◽  
Zachary M. Wilseck ◽  
Thomas L. Chenevert ◽  
Guohua Xi ◽  
Richard F. Keep ◽  
...  
Keyword(s):  
White Matter ◽  
Intracerebral Hemorrhage ◽  
Iron Overload ◽  
The Relationship

scholarly journals Lower functional hippocampal redundancy in mild cognitive impairment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stephanie Langella ◽  
◽  
Muhammad Usman Sadiq ◽  
Peter J. Mucha ◽  
Kelly S. Giovanello ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Cognitive Decline ◽  
Resting State ◽  
Memory Performance ◽  
Potential Mechanism ◽  
Network Efficiency ◽  
Cognitively Normal ◽  
Normal Individuals ◽  
The Relationship

AbstractWith an increasing prevalence of mild cognitive impairment (MCI) and Alzheimer’s disease (AD) in response to an aging population, it is critical to identify and understand neuroprotective mechanisms against cognitive decline. One potential mechanism is redundancy: the existence of duplicate elements within a system that provide alternative functionality in case of failure. As the hippocampus is one of the earliest sites affected by AD pathology, we hypothesized that functional hippocampal redundancy is protective against cognitive decline. We compared hippocampal functional redundancy derived from resting-state functional MRI networks in cognitively normal older adults, with individuals with early and late MCI, as well as the relationship between redundancy and cognition. Posterior hippocampal redundancy was reduced between cognitively normal and MCI groups, plateauing across early and late MCI. Higher hippocampal redundancy was related to better memory performance only for cognitively normal individuals. Critically, functional hippocampal redundancy did not come at the expense of network efficiency. Our results provide support that hippocampal redundancy protects against cognitive decline in aging.

scholarly journals MUC1 and MUC5AC Acting on Helicobacter pylori-Related Deficiency and Solid Syndrome of Spleen and Stomach

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Ling Hu ◽  
Wanqun Chen ◽  
Ming Cheng ◽  
Ting Zhang ◽  
Shaoyang Lan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Dynamic Equilibrium ◽  
Deficiency Syndrome ◽  
Blue Staining ◽  
Significant Difference ◽  
Tai Ji ◽  
Hematoxylin And Eosin ◽  
Relationship Of ◽  
Heat Syndrome ◽  
The Relationship

To investigate the relationship of MUC1, MUC5AC, and the syndrome of spleen and stomach, 109 subjects (34 peptic ulcer (PU), 62 chronic gastritis (CG), and 13 healthy volunteers (CON)) were included. All the subjects included were surveyed with questionnaire to classify them into damp-heat syndrome of spleen and stomach (DHSS), spleen-qi deficiency syndrome (SQD), and CON, examined by gastric endoscope, and biopsied. Rapid urease and methylene blue staining (MBS) were performed on every subject to diagnose for Helicobacter pylori (Hp) infection, and both were defined as Hp-positive. Hematoxylin and eosin (HE) staining was performed on every specimen to explore the histomorphology, inflammatory degree, and inflammatory activity of different groups; then Elivision™ plus kit was used to test the expression of MUC1 and MUC5AC. All the results of digital images were reviewed by two experts blindly. The inflammatory degree with Hp infection was higher than those uninfected or CON, but no significant difference was found between DHSS and SQD. And the expressions of MUC5AC with positive Hp was higher than those with negative Hp or CON regardless of the deficiency and solid syndrome of spleen-stomach but not for MUC1. We speculate that the deficiency and solid syndrome of spleen-stomach is a condition like Tai Ji symbol of dynamic equilibrium, showing the higher expression of MUC5AC but no change of MUC1 in the circumstance of Hp infection.

Ultrasonic Adaptive Sound Speed Estimation for the Diagnosis and Quantification of Hepatic Steatosis: A Pilot Study

2018 ◽  
Vol 40 (06) ◽  
pp. 722-733 ◽  
Author(s):  
Marco Dioguardi Burgio ◽  
Marion Imbault ◽  
Maxime Ronot ◽  
Alex Faccinetto ◽  
Bernard E. Van Beers ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Sound Speed ◽  
Validation Cohort ◽  
Liver Steatosis ◽  
Receiver Operating Curve ◽  
Coefficient Of Determination ◽  
Proton Density ◽  
Speed Estimation ◽  
Training Cohort ◽  
Receiver Operating Curve Analysis

Abstract Purpose To evaluate the ability of a new ultrasound (US) method based on sound speed estimation (SSE) with respect to the detection, quantification, and grading of hepatic steatosis using magnetic resonance (MR) proton density fat fraction (PDFF) as the reference standard and to calculate one US fat index based on the patient’s SSE. Materials and Methods This study received local IRB approval. Written informed consent was obtained from patients. We consecutively included N = 50 patients as the training cohort and a further N = 50 as the validation cohort who underwent both SSE and abdominal MR. Hepatic steatosis was classified according to MR-PDFF cutoffs as: S0 ≤ 6.5 %, S1 6.5 to 16.5 %, S2 16.5 to 22 %, S3 ≥ 22 %. Receiver operating curve analysis was performed to evaluate the diagnostic performance of SSE in the diagnosis of steatosis (S1–S3). Based on the optimal data fit derived from our study, we proposed a correspondence between the MR-PDFF and a US fat index. Coefficient of determination R2 was used to evaluate fit quality and was considered robust when R2 > 0.6. Results The training and validation cohorts presented mean SSE values of 1.570 ± 0.026 and 1.568 ± 0.023 mm/µs for S0 and 1.521 ± 0.031 and 1.514 ± 0.019 mm/µs for S1–S3 (p < 0.01) patients, respectively. An SSE threshold of ≤ 1.537 mm/µs had a sensitivity of 80 % and a specificity of 85.7 % in the diagnosis of steatosis (S1-S3) in the training cohort. Robust correspondence between MR-PDFF and the US fat index was found both for the training (R2 = 0.73) and the validation cohort (R2 = 0.76). Conclusion SSE can be used to detect, quantify and grade liver steatosis and to calculate a US fat index.

scholarly journals MYELODYSPLASTIC SYNDROMES AND IRON CHELATION THERAPY

2017 ◽  
Vol 9 (1) ◽  
pp. e2017021 ◽  
Author(s):  
Emanuele Angelucci ◽  
Silvana Anna Maria Urru ◽  
Federica Pilo ◽  
Alberto Piperno
Keyword(s):  
Iron Overload ◽  
Myelodysplastic Syndromes ◽  
Tissue Damage ◽  
Clinical Evidence ◽  
New Technologies ◽  
Iron Chelation ◽  
Organ Damage ◽  
Evidence Base ◽  
Toxic Damage ◽  
The Relationship

Over recent decades we have been fortunate to witness the advent of new technologies and of an expanded knowledge and application of chelation therapies to the benefit of patients with iron overload. However, extrapolation of learnings from thalassemia to the myelodysplastic syndromes (MDS) has resulted in a fragmented and uncoordinated clinical evidence base. We’re therefore forced to change our understanding of MDS, looking with other eyes to observational studies that inform us about the relationship between iron and tissue damage in these subjects. The available evidence suggests that iron accumulation is prognostically significant in MDS, but levels of accumulation historically associated with organ damage (based on data generated in the thalassemias) are infrequent. Emerging experimental data have provided some insight into this paradox, as our understanding of iron-induced tissue damage has evolved from a process of progressive bulking of organs through high-volumes iron deposition, to one of ‘toxic’ damage inflicted through multiple cellular pathways. Damage from iron may therefore occur prior to reaching reference thresholds, and similarly, chelation may be of benefit before overt iron overload is seen. In this review, we revisit the science and clinical evidence for iron overload in MDS to better characterise the iron overload phenotype in these patients, which is distinct from the classical transfusional and non-transfusional iron overload syndrome. We hope this will provide a conceptual framework to better understand the complex associations between anemia, iron and clinical outcomes, to accelerate progress in this area.

Sign in / Sign up

Export Citation Format

Share Document