scholarly journals A Therapeutic Approach for Wound Healing by Using Essential Oils ofCupressusandJuniperusSpecies Growing in Turkey

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ibrahim Tumen ◽  
Ipek Süntar ◽  
Hikmet Keleş ◽  
Esra Küpeli Akkol
Keyword(s):  
Wound Healing ◽  
Essential Oils ◽  
Capillary Permeability ◽  
Folk Medicine ◽  
Histopathological Analysis ◽  
Hydroxyproline Content ◽  
Wound Healing Effect ◽  
Anti Inflammatory ◽  
Wound Healing Activity

JuniperusandCupressusgenera are mainly used as diuretic, stimulant, and antiseptic, for common cold and wound healing in Turkish folk medicine. In the present study, essential oils obtained from cones ofCupressusand berries ofJuniperuswere evaluated for their wound healing and anti-inflammatory effects.In vivowound healing activity was evaluated by linear incision and circular excision experimental wound models, assessment of hydroxyproline content, and subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol. Additionally acetic-acid-induced capillary permeability test was used for the oils' anti-inflammatory activity. The essential oils ofJ. oxycedrussubsp.oxycedrusandJ. phoeniceademonstrated the highest activities, while the rest of the species did not show any significant wound healing effect. The experimental study revealed thatJ. oxycedrussubsp.oxycedrusandJ. phoeniceadisplay remarkable wound healing and anti-inflammatory activities, which support the folkloric use of the plants.

IN VIVO EVALUATION OF WOUND HEALING AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF ROOTS OF CENTAUREA AFRICANA (L.) IN TOPICAL FORMULATION

2016 ◽  
Vol 10 (1) ◽  
pp. 341 ◽  
Author(s):  
Mohamed Zaouani ◽  
Arezki Bitam ◽  
Ahcen Baz ◽  
Yasmine Benali ◽  
Meriem Hind Ben-mahdi
Keyword(s):  
Wound Healing ◽  
Methanolic Extract ◽  
Wound Contraction ◽  
Inflammatory Activity ◽  
Hydroxyproline Content ◽  
Topical Formulation ◽  
In Vivo Evaluation ◽  
Anti Inflammatory ◽  
Wound Healing Activity

ABSTRACTObjective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.

In Vivo Wound Healing Activity of Spirulina platensis

2019 ◽  
Vol 18 (1) ◽  
pp. 06-16
Author(s):  
R. Seghiri ◽  
A. Essamri
Keyword(s):  
Wound Healing ◽  
Spirulina Platensis ◽  
Therapeutic Agent ◽  
Conventional Medicine ◽  
Folk Medicine ◽  
Chemical Burns ◽  
Amount Of Substance ◽  
Test Population ◽  
Wound Healing Activity

Spirulina is a microalga used in traditional folk medicine in Morocco for the treatment of various health disorders. The wound healing activity of Moroccan Spirulina is unknown. In the current study, aqueous extracts of Spirulina platensis were investigated for acute toxicity and wound healing activity in Swiss Albino mice and White New Zealand rabbits, respectively. The LD50 (amount of substance required to kill 50% of the test population) of the microalga was greater than 5,000 mg/kg. Healing after application of the same amount of ointment on differently induced (mechanical, chemical, and thermal) wounds was about the same, over five weeks. Aqueous extract had remarkable healing activity on rabbits’ skin, possessing significantly greater healing effect for mechanical and chemical burns than controls. Moreover, the hair growing time was faster in treated groups; Spirulina-treated groups did not show any contamination with microbes compared to others. This study affirms that Spirulina platensis can be considered as a potential therapeutic agent for wound healing not only as a complementary medicine but also in conventional medicine.

scholarly journals Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2488
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Fibroblast Cell ◽  
In Vitro Tests ◽  
Low Concentrations ◽  
In Vivo Tests ◽  
Wound Healing Activity

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

scholarly journals WOUND HEALING EFFECT OF KING ALFERD’S MUSHROOM (DALDINIA CONCENTRICA) USED BY TRIBES OF SIRUMALAI HILLS, TAMILNADU, INDIA

2017 ◽  
Vol 9 (7) ◽  
pp. 161 ◽  
Author(s):  
Rajeshwaran Thangaraj ◽  
Sumathy Raj ◽  
Kumuthakalavalli Renganathan
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Field Trips ◽  
Traditional Use ◽  
Wound Healing Effect ◽  
Phytochemical Constituents ◽  
Daldinia Concentrica ◽  
Wound Healing Activity ◽  
Mushroom Sample

Objective: The present investigation is an attempt to an ethnomycological mushroom survey carried out among the paliyar tribals of five settlements in Sirumalai hills. Daldinia concentrica, mushroom reported by the tribals for the wound healing activity was evaluated in vivo in rat model. Methods: The indigenous information of the tribal people was collected through personal interviews during field trips and also the ethno reported mushroom Daldinia concetrica was analysed for its phytochemical constituents by Harborne method and its potential wound healing activity in Albino Wistar rats by excision wound model.Results: The exploration revealed that 92% of the respondents consume mushroom as food and four mushrooms are used in ethnomedicine in the study area namely, Daldinia concentrica, Calvatia gigantean, Termitomyces microcarpus and Podaxis pistillaris. Daldinia concentrica for wound healing, is the first ethnomycological report, thus the further study on Daldinia revealed that the mushroom sample consists of alkaloid 0.97±0.07 mg/kg, flavanoid 2.97±0.12 mg/kg, phenol 0.32±0.01 mg/kg, tannin 0.17±0.02 mg/kg, terpenoid 0.07±0.01 mg/kg and saponin 0.09±0.02 mg/kg; and it’s in vivo wound healing activity was found to be outstanding around 75–87% compared to that of the commercial ointment neosporin.Conclusion: We believe that wound healing activity of Daldinia mushroom was due to the synergestic effect of phytochemicals present in them. Our results throw in the insight of the traditional use of mushrooms in various treatments.

scholarly journals DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL

2019 ◽  
pp. 196-201
Author(s):  
RAM NARENDRAN R ◽  
MALEEKA BEGUM SF ◽  
RUBAVATHI S
Keyword(s):  
Wound Healing ◽  
Drug Carrier ◽  
In Vitro Cytotoxicity ◽  
Toxic Substances ◽  
Cast Film ◽  
In Vivo Animal Model ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model. Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats. Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively. Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.

scholarly journals Evaluation of the Wound Healing Potential ofAchillea biebersteiniiAfan. (Asteraceae) byIn VivoExcision and Incision Models

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Esra Küpeli Akkol ◽  
Ufuk Koca ◽  
Ipek Pesin ◽  
Demet Yilmazer
Keyword(s):  
Wound Healing ◽  
Folk Medicine ◽  
Reference Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Achillea Biebersteinii ◽  
Healing Of Wounds ◽  
Wound Healing Effect ◽  
Incision Wound ◽  
Wound Healing Activity

Achilleaspecies are widely used for diarrhea, abdominal pain, stomachache and healing of wounds in folk medicine. To evaluate the wound healing activity of the plant, extracts were prepared with different solvents; hexane, chloroform, ethyl acetate and methanol, respectively from the roots ofAchillea biebersteinii. Linear incision by using tensiometer and circular excision wound models were employed on mice and rats. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol. Then-hexane extract treated groups of animals showed 84.2% contraction, which was close to contraction value of the reference drug Madecassol (100%). On the other hand the same extract on incision wound model demonstrated a significant increase (40.1%) in wound tensile strength as compared to other groups. The results of histoptological examination supported the outcome of linear incision and circular excision wound models as well. The experimental data demonstrated thatA. biebersteiniidisplayed remarkable wound healing activity.

scholarly journals In Vivo Evaluation of the Wound Healing Activity of Extracts and Bioactive Constituents of the Marine Isopod Ceratothoa oestroides

Marine Drugs ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 219 ◽  
Author(s):  
Evgenia Sofrona ◽  
Leto-Aikaterini Tziveleka ◽  
Maria Harizani ◽  
Panagiota Koroli ◽  
Ioannis Sfiniadakis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Injury ◽  
Healing Process ◽  
Transepidermal Water Loss ◽  
Histopathological Analysis ◽  
Skin Thickness ◽  
Bioactive Constituents ◽  
In Vivo Evaluation ◽  
Wound Healing Activity

Wound healing is a fundamental response to tissue injury and a number of natural products has been found to accelerate the healing process. Herein, we report the preparation of a series of different polarity (organic and aqueous) extracts of the marine isopod Ceratothoa oestroides and the in vivo evaluation of their wound healing activity after topical administration of ointments incorporating the various extracts on wounds inflicted on SKH-hr1 hairless mice. The most active extract was fractionated for enrichment in the bioactive constituents and the fractions were further evaluated for their wound healing activity, while their chemical profiles were analyzed. Wound healing was evaluated by clinical assessment, photo-documentation, histopathological analysis and measurement of biophysical skin parameters, such as transepidermal water loss (TEWL), hydration, elasticity, and skin thickness. The highest levels of activity were exerted by treatment of the wounds with a fraction rich in eicosapentaenoic acid (EPA), as well as myristic and palmitoleic acids. Topical application of the bioactive fraction on the wounds of mice resulted in complete wound closure with a skin of almost normal architecture without any inflammatory elements.

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