scholarly journals In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Fernanda G. Basso ◽  
Taisa N. Pansani ◽  
Ana Paula S. Turrioni ◽  
Vanderlei S. Bagnato ◽  
Josimeri Hebling ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Cell Metabolism ◽  
Statistical Significance ◽  
Cell Number ◽  
Nonparametric Tests ◽  
Low Level Laser Therapy ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast

The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable;780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.

scholarly journals Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tatcha Chaitrakoonthong ◽  
Ruchanee Ampornaramveth ◽  
Paksinee Kamolratanakul
Keyword(s):  
Wound Healing ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Cell Viability ◽  
Real Time ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Migration ◽  
Matrix Gene

Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. The aim of this study was to investigate the rinsing effect of vitamin C on gingival fibroblast behavior utilizing an in vitro wound healing model. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. The rinsing effect of vitamin C on in vitro wound healing was assessed using a scratch test assay. Cell migration, cell viability, and extracellular matrix gene expression were analyzed by transwell migration assay, MTT assay, and real-time RT-PCR, respectively. We found that rinsing with 10 or 20 µg/ml vitamin C significantly increased fibroblast migration (p≤0.05). However, no significant effect was found in the cell viability or in vitro wound healing assays. In contrast, rinsing with 50 µg/ml vitamin C significantly delayed wound closure (p≤0.05). Real-time PCR demonstrated that 50 µg/ml vitamin C significantly increased fibroblast expression of COL1, FN, IL-6, and bFGF. The data demonstrate that rinsing with vitamin C (10/20 µg/ml) accelerates fibroblast migration. However, 50 µg/ml of vitamin C increases the expression of COL1, FN, IL-6, and bFGF, which are related to fibroblast wound healing activity. Prescribing vitamin C with the appropriate duration and drug administration method should be determined to maximize its benefit.

scholarly journals Evaluation of the In Vitro Oral Wound Healing Effects of Pomegranate (Punica granatum) Rind Extract and Punicalagin, in Combination with Zn (II)

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1234 ◽  
Author(s):  
Vildan Celiksoy ◽  
Rachael L. Moses ◽  
Alastair J. Sloan ◽  
Ryan Moseley ◽  
Charles M. Heard
Keyword(s):  
Wound Healing ◽  
Antioxidant Activities ◽  
Punica Granatum ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Anti Inflammatory ◽  
High Concentrations ◽  
And Migration ◽  
Oral Wound Healing

Pomegranate (Punica granatum) is a well-established folklore medicine, demonstrating benefits in treating numerous conditions partly due to its antimicrobial and anti-inflammatory properties. Such desirable medicinal capabilities are attributed to a high hydrolysable tannin content, especially punicalagin. However, few studies have evaluated the abilities of pomegranate to promote oral healing, during situations such as periodontal disease or trauma. Therefore, this study evaluated the antioxidant and in vitro gingival wound healing effects of pomegranate rind extract (PRE) and punicalagin, alone and in combination with Zn (II). In vitro antioxidant activities were studied using DPPH and ABTS assays, with total PRE phenolic content measured by Folin–Ciocalteu assay. PRE, punicalagin and Zn (II) combination effects on human gingival fibroblast viability/proliferation and migration were investigated by MTT assay and scratch wounds, respectively. Punicalagin demonstrated superior antioxidant capacities to PRE, although Zn (II) exerted no additional influences. PRE, punicalagin and Zn (II) reduced gingival fibroblast viability and migration at high concentrations, but retained viability at lower concentrations without Zn (II). Fibroblast speed and distance travelled during migration were also enhanced by punicalagin with Zn (II) at low concentrations. Therefore, punicalagin in combination with Zn (II) may promote certain anti-inflammatory and fibroblast responses to aid oral healing.

scholarly journals Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 138 ◽  
Author(s):  
Chung-Chih Tseng ◽  
Yu-Cheng Lai ◽  
Tsu-Jen Kuo ◽  
Jui-Hsin Su ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Oxidative Phosphorylation ◽  
Marine Biodiversity ◽  
Human Gingival Fibroblast ◽  
Type I ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Expression Levels ◽  
Gene And Protein Expression

Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given the species richness concerning marine biodiversity, it could be a treasure trove for drug discovery. In this study, we demonstrate the effects of a marine compound, (+)-rhodoptilometrin from crinoid, on gingival cell migration, wound healing, and oxidative phosphorylation (OXPHOS). Experimental results showed that (+)-rhodoptilometrin can significantly increase wound healing, migration, and proliferation of human gingival fibroblast cells, and it does not have effects on oral mucosa fibroblast cells. In addition, (+)-rhodoptilometrin increases the gene and protein expression levels of focal adhesion kinase (FAK), fibronectin, and type I collagen, changes the intracellular distribution of FAK and F-actin, and increases OXPHOS and the expression levels of complexes I~V in the mitochondria. Based on our results, we believe that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration. Therefore, (+)-rhodoptilometrin may be a promising therapeutic agent for GR.

scholarly journals Evaluation of Wound Closure Activity ofNigella sativa,Melastoma malabathricum,Pluchea indica, andPiper sarmentosumExtracts on Scratched Monolayer of Human Gingival Fibroblasts

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mas Rizal Ab Rahman ◽  
Fathilah Abdul Razak ◽  
Marina Mohd Bakri
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Nigella Sativa ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Scavenging Activity ◽  
Melastoma Malabathricum

Nigella sativa,Melastoma malabathricum,Pluchea indica, andPiper sarmentosumare common Asian traditional medicines to treat minor wounds. This study aimed to investigate thein vitrowound healing properties of aqueous extracts of these plants using human gingival fibroblast (HGF) monolayer as study model. DPPH scavenging activity of the extracts was evaluated and effect on HGF proliferation was determined. Their effect on HGF’s function to synthesize collagen was indicated by the level of hydroxyproline produced and effect on wound healing activity was assessed using anin vitroscratch assay. The influence of the extracts on expression of bFGF and TGF-βwas also determined. Results revealed all four extracts to exhibit low free radical scavenging activity. The extract fromN. sativa(NSSE) compared to the others showed favourable enhancement of HGF proliferation with EC50of22.67±3.06 µg/mL (P<0.05) with accelerated wound closure activity despite its nonsignificant effect on collagen synthesis. In addition to the elevated level of bFGF by up to 15% at 100 µg/mL of NSSE, a slightly better effect was observed on the expression of TGF-β. NSSE thus showed that promising wound healing properties and data obtained may contribute towards validation of its traditional use for the healing of oral wounds.

scholarly journals Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159843 ◽  
Author(s):  
Nam Cong-Nhat Huynh ◽  
Vincent Everts ◽  
Chidchanok Leethanakul ◽  
Prasit Pavasant ◽  
Ruchanee Salingcarnboriboon Ampornaramveth
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast

In Vitro Biocompatibility of CPP-ACP and Fluoride-containing Desensitizers on Human Gingival Cells

2021 ◽  
Author(s):  
S López-García ◽  
J Guerrero-Gironés ◽  
MP Pecci-Lloret ◽  
MR Pecci-Lloret ◽  
FJ Rodríguez-Lozano ◽  
...  
Keyword(s):  
Cell Migration ◽  
Healing Process ◽  
Oral Care ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Flow Cytometry Data ◽  
In Vitro Biocompatibility ◽  
Induction Of Apoptosis

SUMMARY Objectives: To analyze the biocompatibility of different desensitizers containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride in their composition: MI Varnish (MV), Clinpro White Varnish (3M Oral Care), Profluorid Varnish (VOCO), Duraphat (Colgate) and Embrace Varnish (Pulpdent) on human gingival fibroblast cells (hGF). Methods and Materials: Human gingival fibroblast (hGF) cells were exposed to several desensitizer extracts at different concentrations (0.1%, 1%, and 4% eluates). Then, in vitro biocompatibility was studied by analyzing the IC50 value, cell proliferation (MTT assay and cell cycle), cell migration (wound healing assay), cell morphology and F-actin content (immunocytofluorescence), and induction of apoptosis/necrosis (flow cytometry). Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey test. Results: The lowest cell viability and IC50 were observed in all concentrations of Embrace Varnish-treated hGFs (p&lt;0.001), whereas the highest were exhibited by those treated with Clinpro White Varnish. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were assessed. Finally, MI Varnish, Profluorid Varnish, Duraphat, and Embrace Varnish extracts showed lower numbers of attached cells, some of them with an unusual fibroblastic morphology when cultured with 4% concentration of the varnishes, while Clinpro White Varnish exhibited a similar number of cells with an evident actin cytoskeleton compared to the control group. Conclusions: The results obtained in this study indicate that hGFs show better in vitro biocompatibility after exposure to Clinpro White Varnish, even at the highest concentration employed, making it the most eligible for topical applications. In contrast, Embrace Varnish exhibited a high cytotoxicity towards hGFs that could potentially delay the healing process and regeneration of the oral mucosa, although more studies are needed to confirm this hypothesis.

scholarly journals Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chu-nan Zhang ◽  
Lin-yi Zhou ◽  
Shu-jiao Qian ◽  
Ying-xin Gu ◽  
Jun-yu Shi ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Underlying Mechanism ◽  
Cell Number ◽  
Human Gingival Fibroblasts ◽  
Superior Performance ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Integrin Β1

Abstract Objectives This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. Materials and methods Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis. Results Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism. Conclusion In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant. Clinical significance Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration.

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

Isoniazid-loaded chitosan/carbon nanotubes microspheres promote secondary wound healing of bone tuberculosis

2018 ◽  
Vol 33 (7) ◽  
pp. 989-996 ◽  
Author(s):  
Gangquan Chen ◽  
Yaling Wu ◽  
Dongping Yu ◽  
Rubing Li ◽  
Wenyuan Luo ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Wound Healing ◽  
Cell Number ◽  
Release Time ◽  
Antitubercular Drugs ◽  
Transmission Electron ◽  
Secondary Wound Healing ◽  
Bone Tuberculosis ◽  
Nanoparticle Tracking And Analysis

Poor blood circulation makes it difficult for antitubercular drugs to achieve effective bactericidal concentration at tuberculose focus. The residual Mycobacterium tuberculosis around surgical wound would multiply, resulting in nonunion or sinus formation. Carbon nanotubes have strong tissue penetration and can cross many kinds of physiological barriers. Here, we constructed a chitosan/carbon nanotubes nanoparticles to control slow release of isoniazid. Transmission electron microscopy and nanoparticle tracking and analysis results showed that the diameter of chitosan/carbon nanotubes nanoparticles was between 150 and 250 nm. Chitosan/carbon nanotubes nanoparticles significantly prolonged the release time of isoniazid, and the release rate was more uniform, no sudden release was observed. In vitro experiments showed that chitosan/carbon nanotubes nanoparticles did not destroy biological function of isoniazid, but could reduce its cytotoxicity and inflammation. We further constructed animal model of tuberculous ulcer. The results showed that isoniazid/chitosan/carbon nanotubes nanoparticles promoted the healing of tuberculosis ulcer. Compared with isoniazid group and isoniazid/carbon nanotubes group, the area of wounds decreased by 94.6% and 89.8%, respectively. Immunohistochemistry showed that CD3+ and CD4+ T cell number decreased significantly in isoniazid/chitosan/carbon nanotubes group. In conclusion, we constructed a kind of isoniazid/chitosan/carbon nanotubes nanoparticles, which can significantly promote the healing of tuberculosis ulcer. Our study provided an effective way for the treatment of secondary wound healing of bone tuberculosis.

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