In vitro evaluation of the human gingival fibroblast/gingival mesenchymal stem cell dynamics through perforated guided tissue membranes: cell migration, proliferation and membrane stiffness assay

2017 ◽  
Vol 52 (3) ◽  
pp. 628-635 ◽  
Author(s):  
A. Y. Gamal ◽  
N. N. Al-Berry ◽  
A. A. Hassan ◽  
L. A. Rashed ◽  
V. J. Iacono
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Mesenchymal Stem Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Cell Dynamics ◽  
In Vitro Evaluation ◽  
Membrane Stiffness

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

scholarly journals Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiu-ping Xu ◽  
Hong-li He ◽  
Shu-ling Hu ◽  
Ji-bin Han ◽  
Li-li Huang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Mesenchymal Stem Cell ◽  
Ang Ii ◽  
Stem Cell Migration

scholarly journals Transfect human CCR7 gene in mesenchymal stem cell improves cell migration and anti-apoptosis ability in vitro

Heart ◽  
2011 ◽  
Vol 97 (Suppl 3) ◽  
pp. A45-A45
Author(s):  
H. Xin ◽  
K. Huijia ◽  
W. Lihan ◽  
H. Xinyang ◽  
C. Jiang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Mesenchymal Stem Cell

scholarly journals Interferon Gamma–treated Dental Pulp Stem Cells Promote Human Mesenchymal Stem Cell Migration In Vitro

2015 ◽  
Vol 41 (8) ◽  
pp. 1259-1264 ◽  
Author(s):  
Chelsee Strojny ◽  
Michael Boyle ◽  
Amelia Bartholomew ◽  
Premanand Sundivakkam ◽  
Satish Alapati
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Migration ◽  
Mesenchymal Stem Cell ◽  
Interferon Gamma ◽  
Dental Pulp ◽  
Human Mesenchymal Stem Cell ◽  
Dental Pulp Stem Cells ◽  
Stem Cell Migration

scholarly journals In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Fernanda G. Basso ◽  
Taisa N. Pansani ◽  
Ana Paula S. Turrioni ◽  
Vanderlei S. Bagnato ◽  
Josimeri Hebling ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Cell Metabolism ◽  
Statistical Significance ◽  
Cell Number ◽  
Nonparametric Tests ◽  
Low Level Laser Therapy ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast

The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable;780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.

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