scholarly journals Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Pratima Gupta ◽  
Kalpana Samant ◽  
Avinash Sahu
Keyword(s):  
Filter Paper ◽  
Cellulose Degradation ◽  
Potassium Dichromate ◽  
Gravimetric Method ◽  
Cellulase Activity ◽  
Degrading Bacteria ◽  
Agar Media ◽  
Filter Paper Cellulase ◽  
Simultaneous Saccharification

Eight isolates of cellulose-degrading bacteria (CDB) were isolated from four different invertebrates (termite, snail, caterpillar, and bookworm) by enriching the basal culture medium with filter paper as substrate for cellulose degradation. To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured. CDB 8 and CDB 10 exhibited the maximum zone of clearance around the colony with diameter of 45 and 50 mm and with the hydrolytic value of 9 and 9.8, respectively. The enzyme assays for two enzymes, filter paper cellulase (FPC), and cellulase (endoglucanase), were examined by methods recommended by the International Union of Pure and Applied Chemistry (IUPAC). The extracellular cellulase activities ranged from 0.012 to 0.196 IU/mL for FPC and 0.162 to 0.400 IU/mL for endoglucanase assay. All the cultures were also further tested for their capacity to degrade filter paper by gravimetric method. The maximum filter paper degradation percentage was estimated to be 65.7 for CDB 8. Selected bacterial isolates CDB 2, 7, 8, and 10 were co-cultured withSaccharomyces cerevisiaefor simultaneous saccharification and fermentation. Ethanol production was positively tested after five days of incubation with acidified potassium dichromate.

scholarly journals Isolation of Cellulose-Degrading Endophyte from Capsicum chinense and Determination of its Cellulolytic Potential

2020 ◽  
Vol 10 (6) ◽  
pp. 6964-6973
Keyword(s):  
Filter Paper ◽  
Bacterial Species ◽  
Cellulase Activity ◽  
Bacterial Endophytes ◽  
Capsicum Chinense ◽  
Endoglucanase Activity ◽  
North East India ◽  
North East ◽  
Degrading Bacteria ◽  
Filter Paper Cellulase

Knowledge in the field of bacterial endophytes associated with the plant Capsicum chinense is negligible. So in order to characterize the endophytic population in the targeted plant, different accessions of C. chinense plant were procured from different agro-climatic zones of India. Bacterial endophytes were isolated by using standard protocols. After isolation of the endophytes, a biochemical identification study was performed using the standard key. Secondary metabolites of these bacterial species were studied for their economic importance. One isolate of cellulose-degrading bacteria (CDB) was isolated from the roots of C. chinense. The cellulase activity of the endophyte, containing cellulose Congo Red agar. Finally, enzyme assays for the cellulase (endoglucanase) and filter paper cellulase or FPC assay was studied. The maximum clearing zone for the isolate was 50mm, and the hydrolytic capacity (HC) was found to be 5.96. The endoglucanase activity of 0.95 IU/mL and the filter paper Filter Paper Cellulase (FPCase) activity was found to be 0.25 IU/mL. The importance of the study is attributed to the fact that this is the first-ever study of the enzymatic activity of endophytic bacteria isolated from C. chinense collected from North-East India.

scholarly journals Isolation and Identification of Cellulose Degrading Bacteria from Banana Peel Compost

el–Hayah ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 6-11
Author(s):  
Yendania Grevitara P. ◽  
Badriyatur Rahma F. ◽  
Hellen Septirangga P. ◽  
Irma Dahlia Y. ◽  
Endang Suarsini
Keyword(s):  
Burkholderia Cepacia ◽  
Cellulose Degradation ◽  
Complex Compounds ◽  
Banana Peel ◽  
Identification System ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Isolation And Identification ◽  
Degrading Bacteria

Cellulolytic bacteria are bacteria that have the ability to hydrolyze cellulose complexes into smaller oligosaccharides and eventually become glucose. Glucose is used as a carbon and energy source for bacterial growth. This study was conducted to isolate the cellulose degrading bacteria from banana peel compost that produce cellulose enzymes based on the clear zone that visible around the colony. The cellulolytic activity was determined by the ability of bacteria to hydrolyze the Carboxymethyl Cellulose (CMC) substrate. Determination of cellulolytic activity is known based on cellulolytic index calculation, the diameter total minus the diameter of the colony and divided by the diameter of the colony. The result of five bacterial isolates was found but only one bacterium had the potential to be a cellulose degradation. Based on the Microbact Gram-Negative Identification System, the bacterium is Burkholderia cepacia. These bacteria have an important role in nature as decomposers of various complex compounds, such as cellulose, hemicellulose, lignin, and pectin.

Automated Filter Paper Assay for Determination of Cellulase Activity

2003 ◽  
Vol 107 (1-3) ◽  
pp. 689-704 ◽  
Author(s):  
Stephen R. Decker ◽  
William S. Adney ◽  
Edward Jennings ◽  
Todd B. Vinzant ◽  
Michael E. Himmel
Keyword(s):  
Filter Paper ◽  
Cellulase Activity ◽  
Filter Paper Assay

A simple and fast method for the determination of endo- and exo-cellulase activity in cellulase preparations using filter paper

2012 ◽  
Vol 51 (5) ◽  
pp. 280-285 ◽  
Author(s):  
Marcos Henrique Luciano Silveira ◽  
Martinho Rau ◽  
Elba Pinto da Silva Bon ◽  
Jürgen Andreaus
Keyword(s):  
Filter Paper ◽  
Cellulase Activity ◽  
Fast Method

scholarly journals Hydrolysis of Cellulose by Soluble Clostridium Thermocellum and Acidothermus Cellulolyticus Cellulases

2018 ◽  
Vol 1 (1) ◽  
pp. 5-19
Author(s):  
Phillip Brumm ◽  
Phillip Brumm ◽  
Dan Xie ◽  
Dan Xie ◽  
Larry Allen ◽  
...  
Keyword(s):  
Filter Paper ◽  
Clostridium Thermocellum ◽  
Cellulose Degradation ◽  
Cellulase Activity ◽  
Cellulose Hydrolysis ◽  
Reducing Sugar ◽  
Crystalline Cellulose ◽  
Amorphous Cellulose ◽  
Highly Active ◽  
The Individual

The goal of this work was to clone, express, characterize and assemble a set of soluble thermostablecellulases capable of significantly degrading cellulose. We successfully cloned, expressed, and purified eleven Clostridium thermocellum (Cthe) cellulases and eight Acidothermuscellulolyticus(Acel) cellulases. The performance of the nineteen enzymes was evaluated on crystalline (filter paper) and amorphous (PASC) cellulose. Hydrolysis products generated from these two substrates were converted to glucose using beta-glucosidase and the glucose formed was determined enzymatically. Ten of the eleven Cthe enzymes were highly active on amorphous cellulose. The individual enzymes all produced <10% reducing sugar equivalents from filter paper. Combinations of Cthe cellulases gave higher conversions, with the combination of CelE, CelI, CelG, and CelK converting 34% of the crystalline cellulose. All eight Acel cellulases showed endo-cellulase activity and were highly active on PASC. Only Acel_0615 produced more than 10% reducing sugar equivalents from filter paper, and a combination of six Acel cellulases produced 32% conversion. Acel_0617, a GH48 exo-cellulase, and Acel_0619, a GH12 endo-cellulase, synergistically stimulated cellulose degradation by the combination of Cthe cellulases to almost 80%. Addition of both Acel enzymes to the Cthe enzyme mix did not further stimulate hydrolysis. Cthe CelG and CelI stimulated cellulose degradation by the combination of Acel cellulases to 66%.

Automated Filter Paper Assay for Determination of Cellulase Activity

2003 ◽  
pp. 689-703 ◽  
Author(s):  
Stephen R. Decker ◽  
William S. Adney ◽  
Edward Jennings ◽  
Todd B. Vinzant ◽  
Michael E. Himmel
Keyword(s):  
Filter Paper ◽  
Cellulase Activity ◽  
Filter Paper Assay

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

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