scholarly journals A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine,Angelica sinensis, Using a Proteomic Approach

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Chia-Yen Hsiao ◽  
Ching-Yi Hung ◽  
Tung-Hu Tsai ◽  
Kin-Fu Chak
Keyword(s):  
Wound Healing ◽  
Herbal Medicine ◽  
Angelica Sinensis ◽  
Pharmacological Effects ◽  
Traditional Chinese Herbal Medicine ◽  
Cell Extracts ◽  
Proteomic Approach ◽  
Collagen Secretion ◽  
Biomolecular Assays ◽  
Ethanol Extracts

Angelica sinensis(AS) is a traditional Chinese herbal medicine that has been formulated clinically to treat various form of skin trauma and to help wound healing. However, the mechanism by which it works remains a mystery. In this study we have established a new platform to evaluate the pharmacological effects of total AS herbal extracts as well as its major active component, ferulic acid (FA), using proteomic and biochemical analysis. Cytotoxic and proliferation-promoting concentrations of AS ethanol extracts (AS extract) and FA were tested, and then the cell extracts were subject to 2D PAGE analysis. We found 51 differentially expressed protein spots, and these were identified by mass spectrometry. Furthermore, biomolecular assays, involving collagen secretion, migration, and ROS measurements, gave results that are consistent with the proteomic analysis. In this work, we have demonstrated a whole range of pharmacological effects associated withAngelica sinensisthat might be beneficial when developing a wound healing pharmaceutical formulation for the herbal medicine.

scholarly journals The Great Capacity on Promoting Melanogenesis of Three Compatible Components in Vernonia anthelmintica (L.) Willd.

2021 ◽  
Vol 22 (8) ◽  
pp. 4073
Author(s):  
Yifan Lai ◽  
Qingyuan Feng ◽  
Rui Zhang ◽  
Jing Shang ◽  
Hui Zhong
Keyword(s):  
Herbal Medicine ◽  
Caffeic Acid ◽  
Fluorescent Protein ◽  
Traditional Chinese Herbal Medicine ◽  
Expression Of Genes ◽  
Proliferation And Differentiation ◽  
Green Fluorescent ◽  
Vernonia Anthelmintica

To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of skin depigmentation disorder, we have made use of Vernonia anthelmintica (L.) Willd., a traditional Chinese herbal medicine that has been proven to be effective in treating vitiligo. Here, we report that the extract of Vernonia anthelmintica (L.) Willd. effectively enhances melanogenesis responses in B16F10. In its compound library, we found three ingredients (butin, caffeic acid and luteolin) also have the activity of promoting melanogenesis in vivo and in vitro. They can reduce the accumulation of ROS induced by hydrogen peroxide and inflammatory response induced by sublethal concentrations of copper sulfate in wild type and green fluorescent protein (GFP)-labeled leukocytes zebrafish larvae. The overall objective of the present study aims to identify which compatibility proportions of the medicines may be more effective in promoting pigmentation. We utilized the D-optimal response surface methodology to optimize the ratio among three molecules. Combining three indicators of promoting melanogenesis, anti-inflammatory and antioxidant capacities, we get the best effect of butin, caffeic acid and luteolin at the ratio (butin:caffeic acid:luteolin = 7.38:28.30:64.32) on zebrafish. Moreover, the effect of melanin content recovery in the best combination is stronger than that of the monomer, which suggests that the three compounds have a synergistic effect on inducing melanogenesis. After simply verifying the result, we performed in situ hybridization on whole-mount zebrafish embryos to further explore the effects of multi-drugs combination on the proliferation and differentiation of melanocytes and the expression of genes (tyr, mitfa, dct, kit) related to melanin synthesis. In conclusion, the above three compatible compounds can significantly enhance melanogenesis and improve depigmentation in vivo.

Study of the ESI and APCI interfaces for the UPLC–MS/MS analysis of pesticides in traditional Chinese herbal medicine

2013 ◽  
Vol 406 (5) ◽  
pp. 1481-1491 ◽  
Author(s):  
Lina Chen ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
Zhong Zheng ◽  
Junpeng Xing ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Ms Analysis

Anti-inflammatory and Antioxidant Effects of Japanese Herbal Medicine Kyoseihatekigan on Vocal Fold Wound Healing

2021 ◽  
Author(s):  
Satomi Ozawa ◽  
Shigeyuki Mukudai ◽  
Mami Kaneko ◽  
Shota Kinoshita ◽  
Keiko Hashimoto ◽  
...  
Keyword(s):  
Wound Healing ◽  
Herbal Medicine ◽  
Vocal Fold ◽  
Japanese Herbal Medicine ◽  
Anti Inflammatory ◽  
Antioxidant Effects

Pharmacological Effects of Urinary Products Obtained after Treatment with Saiboku-To, a Herbal Medicine for Bronchial Asthma, on Type IV Allergic Reaction

Planta Medica ◽  
2000 ◽  
Vol 66 (7) ◽  
pp. 607-611 ◽  
Author(s):  
Chizu Taniguchi ◽  
Masato Homma ◽  
Osamu Takano ◽  
Toshihiko Hirano ◽  
Kitaro Oka ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Allergic Reaction ◽  
Bronchial Asthma ◽  
Pharmacological Effects ◽  
Type Iv

scholarly journals In defence of traditional Chinese herbal medicine

Anaesthesia ◽  
2003 ◽  
Vol 58 (3) ◽  
pp. 282-283 ◽  
Author(s):  
I. Hosbach ◽  
G. Neeb ◽  
S. Hager ◽  
S. Kirchhoff ◽  
B. Kirschbaum
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal
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