scholarly journals Quantitative Proteomic (iTRAQ) Analysis of 1st Trimester Maternal Plasma Samples in Pregnancies at Risk for Preeclampsia

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Varaprasad Kolla ◽  
Paul Jenö ◽  
Suzette Moes ◽  
Olav Lapaire ◽  
Irene Hoesli ◽  
...  
Keyword(s):  
At Risk ◽  
Down Syndrome ◽  
Maternal Plasma ◽  
Control Group ◽  
Study Group ◽  
Plasma Samples ◽  
Itraq Analysis ◽  
Galectin 3 ◽  
Healthy Control ◽  
A Current

A current major obstacle is that no reliable screening markers exist to detect pregnancies at risk for preeclampsia. Quantitative proteomic analysis employing isobaric labelling (iTRAQ) has been suggested to be suitable for the detection of potential plasma biomarkers, a feature we recently verified in analysis of pregnancies with Down syndrome foetuses. We have now examined whether this approach could yield biomarkers to screen pregnancies at risk for preeclampsia. In our study, we used maternal plasma samples obtained at 12 weeks of gestation, six from women who subsequently developed preeclampsia and six with uncomplicated deliveries. In our analysis, we observed elevations in 10 proteins out of 64 proteins in the preeclampsia study group when compared to the healthy control group. These proteins included clusterin, fibrinogen, fibronectin, and angiotensinogen, increased levels of which are known to be associated with preeclampsia. An elevation in the immune-modulatory molecule, galectin 3 binding protein, was also noted. Our pilot study, therefore, indicates that quantitative proteomic iTRAQ analysis could be a useful tool for the detection of new preeclampsia screening markers.

Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study

CNS Spectrums ◽  
2017 ◽  
Vol 24 (03) ◽  
pp. 333-337 ◽  
Author(s):  
Maiara Zeni-Graiff ◽  
Adiel C. Rios ◽  
Pawan K. Maurya ◽  
Lucas B. Rizzo ◽  
Sumit Sethi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
At Risk ◽  
Superoxide Dismutase ◽  
High Risk ◽  
Glutathione Peroxidase ◽  
Healthy Control Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Ultra High Risk ◽  
Healthy Control

IntroductionOxidative stress has been documented in chronic schizophrenia and in the first episode of psychosis, but there are very little data on oxidative stress prior to the disease onset.ObjectiveThis work aimed to compare serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in young individuals at ultra-high risk (UHR) of developing psychosis with a comparison healthy control group (HC).MethodsThirteen UHR subjects and 29 age- and sex-matched healthy controls (HC) were enrolled in this study. Clinical assessment included the Comprehensive Assessment of At-Risk Mental States (CAARMS), the Semi-Structured Clinical Interview for DSM-IV Axis-I (SCID-I) or the Kiddie-SADS-Present and Lifetime Version (K-SADS-PL), and the Global Assessment of Functioning (GAF) scale. Activities of SOD and GPx were measured in serum by the spectrophotometric method using enzyme-linked immunosorbent assay kits.ResultsAfter adjusting for age and years of education, there was a significant lower activity of SOD and lower GPX activity in the UHR group compared to the healthy control group (rate ratio [RR]=0.330, 95% CI 0.187; 0.584, p<0.001 and RR=0.509, 95% CI 0.323; 0.803, p=0.004, respectively). There were also positive correlations between GAF functioning scores and GPx and SOD activities.ConclusionOur results suggest that oxidative imbalances could be present prior to the onset of full-blown psychosis, including in at-risk stages. Future studies should replicate and expand these results.

scholarly journals Galectin-3 as a Novel Multifaceted and Not Only Cardiovascular Biomarker in Patients with Psoriasis with Regard to Systemic Treatment—Preliminary Data

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 88
Author(s):  
Anna Baran ◽  
Paulina Kiluk ◽  
Julia Nowowiejska ◽  
Tomasz W. Kaminski ◽  
Magdalena Maciaszek ◽  
...  
Keyword(s):  
Protective Role ◽  
Control Group ◽  
Plaque Type ◽  
Before And After ◽  
Diagnostic Role ◽  
Galectin 3 ◽  
Healthy Control ◽  
History Of ◽  
Cardiovascular Biomarker ◽  
Positive Correlations

Galectin-3 (gal-3) is a multifunctional regulator of various biological processes and diseases, which are common comorbidities in psoriasis. Data regarding potential diagnostic role of gal-3 in psoriasis are insufficient. Serum gal-3 levels were evaluated before and after twelve weeks of treatment with acitretin or methotrexate in 31 patients with plaque-type psoriasis and compared to 11 healthy control group. The mean serum galectin-3 level in patients with psoriasis was significantly higher compared to the control group (p < 0.01). In patients with obesity and long-lasting psoriasis (>20 years) positive relations of gal-3 and PASI were noted. In psoriatics with low gal-3 levels, positive correlations between the gal-3 and BMI, glucose level, and with the latter in short-lasting psoriasis (<20 years) were noted. In the long history of psoriasis, gal-3 was negatively correlated with lipids levels. The Gal-3 level might be a multifaceted modulator of the course of psoriasis and predictive factor of cardiometabolic comorbidities’ development, especially in patients with a long history of the disease or obesity. Patients with low serum gal-3 and short history of psoriasis are presumably at greater risk of diabetes. In patients with long-lasting psoriasis and concomitant obesity, gal-3 may exert a protective role against dyslipidemia or perhaps further CMD development.

scholarly journals Detection of Chromosome 21-encoded mRNA of Placental Origin in Maternal Plasma

2003 ◽  
Vol 49 (9) ◽  
pp. 1445-1449 ◽  
Author(s):  
Cees B M Oudejans ◽  
Attie T J J Go ◽  
Allerdien Visser ◽  
Monique A M Mulders ◽  
Bart A Westerman ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Placental Tissue ◽  
Rna Isolation ◽  
First Trimester ◽  
Maternal Plasma ◽  
Chromosome 21 ◽  
Placental Lactogen ◽  
Plasma Samples ◽  
Human Placental Lactogen ◽  
Dnase Treatment

Abstract Background: mRNA of placental origin (i.e., human placental lactogen and β-human chorionic gonadotropin) has been demonstrated to be easily detectable in maternal plasma. We tested whether detection of chromosome 21-encoded mRNA of placental origin is possible in maternal plasma obtained during the first trimester. Methods: Plasma samples were obtained from pregnant women between weeks 9–13 of pregnancy. RNA was isolated from 800 or 1600 μL of plasma by silica-based affinity isolation and, after on-column DNase treatment, was subjected to two-step, one-tube reverse transcription-PCR with gene specific primers. Results: Three chromosome 21-encoded genes located within the Down syndrome critical region with overexpression in trisomy 21 placentas were screened for expression in early placental tissue to select their potential use for RNA based plasma screening. One of the chromosome 21-encoded genes (LOC90625) showed strong expression in first trimester placenta similar to CSH1 (human placental lactogen) and was selected for plasma analysis. The RNA isolation assay was validated with CSH1 mRNA, which could be detected in the plasma of all women tested in weeks 9–13 of pregnancy. RNA from the chromosome 21-encoded, placentally expressed gene, LOC90625, was present in maternal first-trimester plasma and could be detected in 60% of maternal plasma samples when 800 μL of plasma was used and in 100% of samples when 1600 μL of plasma was used. Conclusion: The detection of chromosome 21-encoded mRNA of placental origin in maternal plasma during the first trimester may allow development of plasma-RNA-based strategies for prenatal prediction of Down syndrome. LOC90625 is a candidate gene for this purpose.

Prognostic value of mean platelet volume on tinnitus

2015 ◽  
Vol 130 (2) ◽  
pp. 162-165 ◽  
Author(s):  
O Kemal ◽  
T Müderris ◽  
F Başar ◽  
G Kutlar ◽  
F Gül
Keyword(s):  
Mean Platelet Volume ◽  
Healthy Subjects ◽  
Clinical Findings ◽  
University Hospital ◽  
Control Group ◽  
Study Group ◽  
Pathophysiological Mechanism ◽  
Platelet Volume ◽  
Significant Difference ◽  
Healthy Control

AbstractObjective:This study aimed to determine whether there was any relationship between tinnitus and mean platelet volume.Methods:This retrospective study was conducted between January 2013 and January 2014 in Ankara Atatürk Hospital and Ondokuz Mayıs University Hospital, Turkey, on a study group of 86 patients with tinnitus and a control group of 84 healthy subjects. Mean platelet volume was recorded and comparisons were made between the two groups.Results:Mean (± standard deviation) platelet volume was 7.67 ± 0.83 μm3 in the study group and 7.28 ± 0.56 μm3 in the control group. There was a statistically significant difference in mean platelet volume between the tinnitus patients and the healthy subjects (p < 0.05).Conclusion:The clinical findings indicated that tinnitus patients had a higher mean platelet volume than the healthy control subjects; however, the pathophysiological mechanism remains unclear.

scholarly journals Association of Low Serum Cholesterol With Hemorrhagic Stroke

KYAMC Journal ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 147-152
Author(s):  
ABM Khairul Hasan ◽  
Jalal Uddin ◽  
Safayet Ahmed ◽  
Kazi Shihab Uddin ◽  
Muhammad Alamgir Mandal ◽  
...  
Keyword(s):  
Serum Cholesterol ◽  
Hemorrhagic Stroke ◽  
Total Cholesterol Level ◽  
Control Group ◽  
Study Group ◽  
College Hospital ◽  
Medical College ◽  
Healthy Control ◽  
The Difference ◽  
Low Serum

Background: Stroke is the second cause of death and third cause of disability in worldwide so control of risk factor is the best option for prevention of stroke and stroke related disability. Objectives: The study is to know the relation of low serum cholesterol with hemorrhagic stroke. Materials and Methods: This Study is a case control study carried out at Neurology and Medicine ward Mymensingh Medical college hospital (MMCH) from july2017 to December 2018 to see the association of low serum cholesterol with hemorrhagic stroke. A total number of 120 subjects more than 18 years were considered for the study. Among them 60 were study group and 60 were control group. The study population has been selected following some exclusion and inclusion criteria. Results: Result showed that hemorrhagic stroke was more in male than female, common after the age of 60 years. Lower middle classes are more sufferers. Hypertension was 76.7% of study group and serum cholesterol was significantly lower in study group than the control group. Conclusion: This study found that the serum total cholesterol level of study group is less than that of the healthy control group and the difference is statistically highly significant (p< 0.001). So it can be concluded that low serum cholesterol is associated with hemorrhagic stroke. KYAMC Journal. 2021;12(3): 147-152

scholarly journals The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome

2020 ◽  
Vol 9 (12) ◽  
pp. 3995
Author(s):  
Angelika Buczyńska ◽  
Iwona Sidorkiewicz ◽  
Sławomir Ławicki ◽  
Adam Krętowski ◽  
Monika Zbucka-Krętowska
Keyword(s):  
Down Syndrome ◽  
Apolipoprotein E ◽  
High Sensitivity ◽  
Control Group ◽  
Study Group ◽  
Diagnostic Efficiency ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Noninvasive Biomarker ◽  
Medical University

Prenatal screening for Down syndrome (DS) is based on both noninvasive and invasive methods. Noninvasive, cell-free fetal DNA genetic tests are expensive, whereas biochemical methods remain imprecise. Amniocentesis is the most frequently used invasive diagnosis procedure, characterized by 99.8% diagnostic efficiency and less than 1% risk of miscarriage. The aim of this study was to evaluate the screening value of apolipoprotein E (ApoE) as a potential noninvasive biomarker for prenatal DS assessment. This study was conducted on a group of female patients who decided to undergo routine amniocentesis between the 15th and 18th week of pregnancy at the Department of Reproduction and Gynecological Endocrinology of the Medical University of Bialystok, Poland. For the purpose of this study, 20 women with DS fetuses were selected as the study group, and 20 healthy pregnant women with euploid fetus karyotypes as the control group. The plasma levels of ApoE were significantly higher in the study group compared to healthy subjects (p < 0.05). The area under the receiver operating characteristic (ROC) curve was 0.978 (p < 0.001), with the cut-off set to 1.37 mg/mL, which was characterized by 80% of sensitivity and 100% of specificity. The high sensitivity and specificity demonstrate the screening utility of maternal ApoE concentration in prenatal fetal DS screening.

Choroid thickness profile in patients with lupus nephritis

Lupus ◽  
2019 ◽  
Vol 28 (4) ◽  
pp. 475-482 ◽  
Author(s):  
J Braga ◽  
R Rothwell ◽  
M Oliveira ◽  
D Rodrigues ◽  
S Fonseca ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Choroidal Thickness ◽  
Central Nervous System Involvement ◽  
Enhanced Depth Imaging ◽  
Control Group ◽  
Study Group ◽  
Ophthalmological Examination ◽  
Increased Risk ◽  
Healthy Control

Background Choroidopathy is a rare manifestation of systemic lupus erythematosus (SLE). This entity is associated with active phases of severe SLE and it is frequently accompanied by acute kidney failure, central nervous system involvement and coagulopathy. Purpose To evaluate the choroid thickness of patients with lupus nephritis (LN) without choroidopathy, and to compare this with that of age-matched SLE patients without LN and healthy control subjects. Study design Cross-sectional case control study. Material and methods Fifteen women with LN in remission phase (study group), 15 women with SLE in remission without LN (SLE control group), and 15 healthy women (healthy control group), without ocular diseases or significant refractive error, were recruited. Full ophthalmological examination and a macular optical coherence tomography in enhanced depth imaging mode were performed. The choroid thickness was measured at nine macular points and six lines of mean choroidal thickness were determined. A comparative analysis between the three groups was performed using the one-way ANOVA test and the paired t-test. The choroid thickness of patients under corticotherapy was also compared to that of patients without corticotherapy. Additionally, the correlation between choroid thickness and disease duration was evaluated using the Pearson analysis. Results The mean macular choroidal thickness was 295.73 ± 67.62 μm in the study group, 233.34 ± 41.01 µm in the SLE control group, and 240.98 ± 37.93 μm in the control group ( p = 0.00006 and p = 0.0003, respectively). Additionally, the choroid thickness was significantly thicker than in the SLE and healthy control groups at the foveal ( p = 0.004 and p < 0.000), nasal ( p < 0.000 and p = 0.001), superior ( p = 0.002 and p < 0.000) and inferior ( p < 0.000 and p = 0.001) mean lines. The choroidal thickness in this group was not associated with the duration of the disease. The subgroup of patients with LN under corticotherapy did not reveal a significantly different choroidal thickness. Conclusion This study suggests a relationship between LN and choroidal changes, which may represent an increased risk for choroidopathy in these patients. Choroid thickening was not related with the duration of the disease. This thickening may be correlated with histopathological changes similar to those occurring in kidney glomeruli.

Activity of eosinophils and immunoglobulin E concentration in the peritoneal fluid of women with endometriosis

2004 ◽  
Vol 42 (6) ◽  
Author(s):  
Androne Eidukaite ◽  
Vytas Tamosiunas
Keyword(s):  
Flow Cytometry ◽  
Enzyme Immunoassay ◽  
Peripheral Blood ◽  
Peritoneal Fluid ◽  
Immunoglobulin E ◽  
Abdominal Cavity ◽  
Control Group ◽  
Study Group ◽  
Autoantibody Production ◽  
Healthy Control

AbstractAutoinflammatory phenomena, including autoantibody production and atopy, have been regarded as associated with endometriosis. The present study investigates the activity of eosinophils and the distribution of immunoglobulin E concentrations in the peritoneal fluid of women with early endometriosis. The study group consisted of 30 patients with laparoscopically diagnosed early endometriosis. The healthy control group consisted of 18 females with no evident changes in the abdominal cavity and no endometrial foci. Concentrations of immunoglobulin E in serum and peritoneal blood were determined by enzyme immunoassay. The activity of eosinophils was estimated according to the expression of the early activation molecule CD69 by the flow cytometry method. The concentrations of immunoglobulin E in the peripheral blood and peritoneal fluid were similar in both groups. However, the count of CD69+ eosinophils was higher in the peritoneal fluid of women with endometriosis. The results indicate that activated eosinophils accumulate in the peritoneal fluid in early endometriosis and can play a significant role in the pathogenesis of the disease.

scholarly journals Association of goals of care meetings for hospitalized cancer patients at risk for critical care with patient outcomes

2014 ◽  
Vol 29 (4) ◽  
pp. 386-390 ◽  
Author(s):  
Colleen C Apostol ◽  
Julie M Waldfogel ◽  
Elizabeth R Pfoh ◽  
Donald List ◽  
Lynn S Billing ◽  
...  
Keyword(s):  
Health Care ◽  
At Risk ◽  
Critical Care ◽  
Health Care Utilization ◽  
Cancer Patients ◽  
Care Utilization ◽  
Control Group ◽  
Study Group ◽  
Comfort Care ◽  
Goals Of Care

Background: Caring for cancer patients with advanced and refractory disease requires communication about care preferences, particularly when patients become ill enough to be at risk for critical care interventions potentially inconsistent with their preferences. Aim: To describe the use of goals of care discussions in patients with advanced/refractory cancer at risk for critical care interventions and evaluate associations between these discussions and outcomes. Design: Cohort study describing patients/families’ perceptions of goals of care meetings and comparing health care utilization outcomes of patients who did and did not have discussions. Setting/participants: Inpatient units of an academic cancer center. Included patients had metastatic solid tumors or relapsed/refractory lymphoma or leukemia and were at risk for critical care, defined as requiring supplemental oxygen and/or cardiac monitor. Results: Of 86 patients enrolled, 34 (39%) had a reported goals of care discussion (study group). Patients/families reported their needs and goals were addressed moderately to quite a bit during the meetings. Patients in the study group were less likely to receive critical care (0% vs 22%, p = 0.003) and more likely to be discharged to hospice (48% vs 30%, p = 0.04) than the control group. Only one patient in the study group died during the index hospitalization (on comfort care) (3%) compared with 9(17%) in the control group ( p = 0.08). Conclusion: Goals of care meetings for advanced/refractory cancer inpatients at risk for critical care interventions can address patient and family goals and needs and improve health care utilization. These meetings should be part of routine care for these patients.

scholarly journals EVALUATION OF GALECTIN-3 LEVELS IN OBESE ADOLESCENTS

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Hüseyin Dag ◽  
Nevin Cetin Dag ◽  
Emine Turkkan ◽  
Ebru Misirli Ozdemir ◽  
Okan Dikker
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Obese Adolescent ◽  
Adolescent Group ◽  
Obese Adolescents ◽  
Significant Difference ◽  
Galectin 3 ◽  
Healthy Control

Background: Galectin-3 is a member of the family of soluble beta-galactoside binding lectins, which is involved in inflammation, cell differentiation, adhesion, proliferation, angiogenesis and cancer cell invasion. Increased galectin-3 levels in obese patients have been shown in previous studies. In our study; we aimed to investigate the galectin-3 levels in obese adolescents for the first time in the literature. Materials and methods: A total of 85 adolescent patients who were admitted to our pediatrics outpatient clinic between December 19th of 2018 and January 31st of 2019 were included in our study. Among those patients 52 were obese and 33 were normal-weighted. Adolescents having body mass index (BMI) 95% percentile and above according to their age and sex were defined as obese. Enzyme-Linked Immunosorbent Assay kit was used for the measurement of galectin-3 in serum. Laboratory tests (Galectin-3, HbA1c, glucose, insulin, HOMA-IR), sex, age and BMI were statistically compared between groups. Results: We found no statistically significant difference in terms of gender distribution rates, age, glucose or galectin-3 levels among obese adolescent and healthy control groups (p> 0.05). The BMI, HbA1c, HOMA-IR and insulin levels of the obese adolescent group were found to be significantly higher than the healthy control group (p <0.05). We found no statistically significant correlation between galectin-3 levels and other parameters in obese adolescent group (p> 0.05). Conclusion: In our study, we did not find any relationship between serum galectin-3 levels and obesity in adolescents. We believe that the effects of galectin-3 levels on obesity in adolescents should be more clearly explained by further investigations, which also measures other biomarkers of the mechanism of action. Keywords: Galectin-3, obesity, adolescent List of abbreviations: BMI: Body-Mass Index, CV: Variation coefficient, ELISA: Enzyme linked immunoassay, HOMA-IR: Homeostatic model of assessment-insulin resistance, IL: Interleukin, r: Correlation coefficient, SD: Standard deviation.

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