scholarly journals Antiatherogenic and Anti-Ischemic Properties of Traditional Chinese Medicine Xinkeshu via Endothelial Protecting Function

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Xu Tao ◽  
Peng Jing-bo ◽  
Zhang Wen-tong ◽  
Zhao Xin ◽  
Zhang Tao-tao ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Myocardial Ischemia ◽  
Probable Mechanism ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Diet Model

Including herbal medicine, complementary and alternative medicine (CAM) is popular worldwide. The traditional Chinese medicine xinkeshu has been widely used to treat coronary heart disease in China. This study was designed to investigate the protective effect and probable mechanism of xinkeshu tablet to atherosclerotic myocardial ischemia rabbit. Rabbits were divided into four groups (n=12each) and fed with different diet for 12 weeks: Control (standard diet), Model (high-cholesterol diet), XKS (high-cholesterol diet with 184.8 mg/kg/d xinkeshu), and Atorvastatin (high-cholesterol diet with 5.0 mg/kg/d atorvastatin). Plasma lipoprotein, ECG, endothelium-dependent vessel relaxation, histomorphological study, and expressions of eNOS and VCAM-1 on coronary arteries were assessed. The findings showed that, similar to atorvastatin, xinkeshu presented significant effects on rescuing endothelium-dependent vessel relaxation, inhibiting atherosclerotic progress, preventing myocardial ischemia, and changing eNOS and VCAM-1 expression. However, xinkeshu showed no lipoprotein lowering effect in hypercholesterolemia rabbits. The results of the present study indicated that xinkeshu exerted potent antiatherogenic and anti-ischemic properties on atherosclerotic myocardial ischemia rabbit. An endothelial protecting effect may be involved in the mechanism other than antihyperlipidemic effect.

scholarly journals Rutin Supplementation on Cholesterol Metabolism in Female Albino Wistar Rats Using High‐Cholesterol‐Diet Model: Possible Renal Protection

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
SALIM S. AL-REJAIE ◽  
Hatem M. Abuohashish ◽  
Abdulaziz S. Alroujayee ◽  
Abdulaziz M. Aleisa ◽  
Osama Alkhamees
Keyword(s):  
Wistar Rats ◽  
Cholesterol Metabolism ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Renal Protection ◽  
Diet Model

scholarly journals POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET

2017 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
Ketut Srie Marhaeni Julyasih ◽  
I Gede Putu Wirawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Design Research ◽  
Wistar Rat ◽  
Negative Control ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.

Hypercholesterolemia Inhibits Angiogenesis in Response to Hindlimb Ischemia

Circulation ◽  
2000 ◽  
Vol 102 (suppl_3) ◽  
Author(s):  
Junli Duan ◽  
Toyoaki Murohara ◽  
Hisao Ikeda ◽  
Atsushi Katoh ◽  
Satoshi Shintani ◽  
...  
Keyword(s):  
Blood Flow ◽  
Dietary Intervention ◽  
Laser Doppler ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
Hindlimb Ischemia ◽  
Sprague Dawley ◽  
High Cholesterol ◽  
Arginine Supplementation

Background —Endothelium-derived nitric oxide (EDNO) plays an important role in the regulation of angiogenesis, whereas hypercholesterolemia (HC) impairs EDNO release. We examined the hypothesis that HC may inhibit ischemia-induced angiogenesis by inhibition of EDNO in a rat model of unilateral hindlimb ischemia and that oral l -arginine supplementation, a substrate for NO synthase, may prevent HC-related impairment of angiogenesis. Methods and Results —Male Sprague-Dawley rats were fed (A) standard diet (control), (B) 2% high-cholesterol diet (HC group), or (C) high-cholesterol diet with oral l -arginine (2.25% in drinking water) (HC+L-arg group). At 2 weeks of the dietary intervention, unilateral limb ischemia was surgically induced in all animals. Dietary HC groups (B and C) revealed elevated total and LDL cholesterol levels compared with control animals. Laser Doppler blood flow analyses showed significant decreases in the ischemic/normal limb blood flow ratio in the HC group compared with controls ( P <0.05) when followed up until 4 weeks after surgery. Selective angiography and immunohistochemical analyses in the ischemic limb at postoperative day 14 revealed significantly lower angiographic scores ( P <0.01) and capillary densities ( P <0.01) in the HC group than controls, which were associated with decreased tissue contents of NO x and cGMP. Oral l -arginine supplementation (HC+L-arg) significantly improved all parameters of the laser Doppler blood perfusion ratio, angiographic scores, and capillary densities ( P <0.01 versus HC group), which were accompanied by significant elevations in serum l -arginine levels and tissue NO x and cGMP contents. Conclusions —Collateral vessel formation and angiogenesis in response to hindlimb ischemia were significantly attenuated in rats with dietary HC. The mechanism may be related to the reduced NO bioactivity in the ischemic tissues. Augmentation of the tissue NO activity by oral l -arginine supplementation restored the impaired angiogenesis in HC.

scholarly journals Effects of Kombucha Tea on Side Effects of High Cholesterol Diet in Rabbits

2020 ◽  
Author(s):  
Monir Doudi ◽  
Zahra Hooshmandi ◽  
Somayeh Saedi ◽  
Mahbubeh Setorki
Keyword(s):  
Side Effects ◽  
Blood Cholesterol ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Pancreatic Acini ◽  
Kombucha Tea ◽  
Significant Difference

Background: This research aimed to assess the effect of kombucha tea on reducing cholesterol (HCH) uptake and ameliorating biochemical and physiological side effects of high cholesterol diets.  Objectives: The aim of this research was evaluate the effectiveness of Kombucha tea in reducing cholesterol uptake as well as pathological and immunological side effects  from high cholesterol diets in Rabbit.  Methods: A total of 32 rabbits were assigned into four groups: 1. Control, fed with standard pellets; 2. HCh+St, fed with high cholesterol diet; 3. HCh+Kom, fed with high cholesterol diet and orally received kombucha beverage; and 4. Kom+St, orally received kombucha beverage along with standard diet. Then, the blood Cholesterol (CHO), Triglycerides (TG), Low-density Lipoprotein (LDL), Malondialdehyde (MDA), ferritin, Creatine Phosphokinase (CPK), Interleukin-1 (IL-1), interleukin-6 (IL-6), and liver enzymes (alanine transaminase [ALT], and aspartate transaminase [ AST]), as well as liver histopathological features, were evaluated in all groups. Results: CHO and TG showed the highest level in the blood of the HCh+St group, whereas those gavaged with HCh+Kom displayed no change in TG and LDL. Conversely, the HCh+Kom group showed a significant increase in HDL when compared with HCh+St and Kom+St groups. MDA ferritin and CPK in the Kom+St group was significantly higher than the other groups, followed by HCh+Kom. No significant difference was observed in the values of IL-1 and IL-6 as well as ALT and AST between the experimental groups and the control group. Histopathological observations of the liver tissues revealed foam cells with an acidophilic appearance in the HCh+St group and hyperemia in the sinusoid of HCh+Kom and Kom+St groups. Degradation in pancreatic acini was observed in the HCh+St rabbits.  Conclusion: The data suggest that kombucha consumption is effective in preventing problems caused by high cholesterol diets.

scholarly journals Effects of cholesterol and Lactobacillus acidophilus on testicular function

2021 ◽  
Vol 48 (3) ◽  
pp. 229-235
Author(s):  
Gülay Çiftci ◽  
Elif Tuna
Keyword(s):  
Lactobacillus Acidophilus ◽  
Testicular Tissue ◽  
Male Rats ◽  
Wall Structure ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Androgen Binding

Objective: In this study, the effects of Lactobacillus acidophilus on testosterone (TES), follicle-stimulating hormone (FSH), luteinizing hormone (LH), androgen-binding protein (ABP), factor-associated apoptosis (FAS), and total cholesterol (TC), as well as histopathological changes, were investigated in male rats fed a high-cholesterol diet. Methods: The study included three groups. The control (C) group was fed standard-diet for 8 weeks. The hypercholesterolemia (HC) group was fed a 2% cholesterol-diet for 8 weeks. The therapeutic group (HCL) was fed a 2% cholesterol-diet for 8 weeks and administered L. acidophilus for the last 4 weeks. FSH, TES, and FAS levels in testicular tissue were determined using an enzyme-linked immunosorbent assay (ELISA), while another sample was examined histopathologically. LH and ABP levels were determined using ELISA, and serum TC levels were assessed via an autoanalyzer. Results: In the HC group, the TC levels were significantly higher and the LH levels were lower (p<0.05) than in the C group. The ABP levels were lower (p>0.05). In the HCL group, the LH and ABP levels were higher (p>0.05) and the TC level significantly lower (p<0.05) than in the HC group. The TES and FSH levels were lower, and the FAS levels were higher, in the HC than in the C group (p<0.05). In the HCL group, levels of all 3 resembled control levels. Histologically, in the testicular tissue of the HC group, the cells in the tubular wall exhibited atrophy, vacuolization, and reduced wall structure integrity. However, in the HCL group, these deteriorations were largely reversed.Conclusion: Supplementary dietary administration of an L. acidophilus to hypercholesterolemic male rats positively impacted testicular tissue and male fertility hormone levels.

Antihypercholesterolemic and anti-atherogenic effects of lyophilized aqueous extract of Globularia alypum leaves in rats fed a high-cholesterol diet

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Nawal Taleb-Dida ◽  
Djamil Krouf ◽  
Yasmina Bahlil ◽  
Sarra Dali ◽  
Fatima Zohra Alachaher ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Atherogenic Index ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Lcat Activity ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Apo B ◽  
Content Type ◽  
Globularia Alypum

Purpose This paper aims to investigate the preventive effects of a concomitant supplementation of a lyophilized aqueous extract of Globularia alypum (Ga) leaves in a high cholesterol-diet (HC-D) on lipid profile and lecithin cholesterol acyltransferase (LCAT) activity in hypercholesterolemic rats. Design/methodology/approach Twenty-four male Wistar rats weighing 232 ± 10 g were divided into four groups (n = 6). Two control groups were fed a standard-diet (St-D) supplemented (C-Ga) or not (C) with 1.66% Ga leaf extract. The two others experimental groups were fed HC-D, which contains the St-D plus 1% of cholesterol and 0.5% of cholic acid supplemented (HC-Ga) or not (HC) with the same amount of Ga. At d28, feces were collected and fasting rats were anesthetized; bloods and livers were removed to measure biochemical parameters. Findings In hypercholesterolemic (HC) rats, Ga supplementation in HC-D induced a significant reduction in ALT (−64%, p = 0.002) and AST (−71%; p = 0.005) activities, in plasma TC (−55%; p = 0.03) and TG (−54%; p = 0.01) concentrations, in cholesterol contents of atherogenic lipoproteins VLDL (−78%; p = 0.004) and LDL-HDL1 (−64%; p = 0.003) and inversely, an increase in those of anti-atherogenic HDL2 (+14%; p = 0.002). Feeding the HC-D-Ga exhibited a reduction in atherogenic index Apo B/Apo A-I (−72%; p = 0.002), an increase in faecal lipids, cholesterol excretion and in plasma apo A-I (+60%; p = 0.002) and HDL2-cholesteryl esters (+32%, p = 0.04) and then improved LCAT activity (+31%; p = 0.03). Originality/value In hypercholesterolemic rats, Globularia alypum extract was effective in preventing lipid disorders by its hypolipidemic action, had an anti-atherogenic potential and a protective effect against cardiovascular risk by enhancing LCAT activity.

scholarly journals The Effect of Tualang Honey in Chronic Exposure of High Cholesterol Diet in Animal Model

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Roslina Abdul Rahim ◽  
Siti Aesah @ Naznin Muhammad ◽  
Azril Shahreez Abdul Ghani ◽  
Nor Zamzila Abdullah ◽  
Norlelawati A. Talib ◽  
...  
Keyword(s):  
Animal Model ◽  
Renal Function ◽  
Histological Examination ◽  
Chronic Exposure ◽  
Lipid Lowering ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Tualang Honey

Introduction: Hyperlipidaemia accompanies chronic renal disease either as a consequence of the renal dysfunction or as part of generalized metabolic derangements. The aim of this study was to determine the effects of tualang honey (TH) on the kidneys of animal model with chronic exposure to high cholesterol diet.  Materials and method:  Twenty Sprague-Dawley rats were divided into two groups, the high cholesterol diet (12% CD (n= 16) and standard diet (SD) (n=4) and were fed for 12 weeks. After 12 weeks, the rats in the 12% CD group were subsequently divided into four groups. The first group was continued with only 12% CD while the other 3 groups in addition to the 12% CD, they were given TH treatment at different concentrations (1.2, 2.4 and 3.0 g/kg/day) for 4 weeks. Biochemical analysis of lipid profile and renal function were performed at the end of the experiment. The animals were sacrificed and the kidneys were harvested for histological examination.  Results:  In the 12 weeks HCD group, the serum cholesterol, LDL-c and creatinine levels were significantly higher) compared to that of the SD group. All groups with the tualang honey treatment had significant reduction in the LDL-c, triglyceride and creatinine levels. Histological examination of the kidney tissues of the HCD and HCD+TH groups at 16 weeks revealed segmental mesangial proliferation of some glomeruli with focal mesangial matrix expansion. No areas of periglomerular and peritubular fibrosis were observed.  Conclusion:  Tualang honey supplementation of animal model with chronic exposure to high cholesterol diet improved the renal function hence suggesting the its renoprotective effect. However, there were no changes seen in the histology of the kidneys . Additionally, tualang honey showed improvement in the LDL-c and triglyceride levels indicating its lipid lowering activities.

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