scholarly journals Preparation and Properties of Biphasic Calcium Phosphate Scaffolds Multiply Coated with HA/PLLA Nanocomposites for Bone Tissue Engineering Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Lei Nie ◽  
Jinping Suo ◽  
Peng Zou ◽  
Shuibin Feng
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Coating Layer ◽  
Multilayer Coating ◽  
High Porosity ◽  
Negative Effects ◽  
Porous Network ◽  
Layer Number

A well-developed BCP scaffolds coated with multilayer of HA/PLLA nanocomposites with interconnectivity, high porosity, and moderate compressive strength as well as good biocompatibility were fabricated for bone tissue engineering. After being multiply coated with HA/PLLA nanocomposites, the scaffolds maintained the BCP framework structure, and the porous network structure of scaffolds remained unchanged; however, the compressive strength was increased with the increase of coating layer number of HA/PLLA nanocomposites. The prepared scaffolds showed lower variation of pH values in SBF solution, and an increase of coating layer number led to the decrease of the biodegradation rate at different days. Moreover, the multilayer coating scaffolds had good cytocompatibility, showing no negative effects on cells growth and proliferation. Furthermore, the bone-like apatite layer was built obviously in the interface of scaffold after 21 days after implantation in SD rat muscle. In conclusion, the BCP scaffold coated with multilayer of HA/PLLA nanocomposites could be a candidate as an excellent substitute for damaged or defect bone in bone tissue engineering.

Strong and Bioactive Tri-Calcium Phosphate Scaffolds with Tube-Like Macropores

2014 ◽  
Vol 19 ◽  
pp. 65-75 ◽  
Author(s):  
Wei Zheng ◽  
Gang Liu ◽  
Cheng Yan ◽  
Yin Xiao ◽  
Xi Geng Miao
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
High Porosity ◽  
Calcium Phosphate Ceramic ◽  
Si Doped ◽  
Butadiene Styrene

Calcium phosphate ceramic scaffolds have been widely investigated for bone tissue engineering due to their excellent biocompatibility and biodegradation. Unfortunately, they have low mechanical properties, which inversely restrict their wide applications in load-bearing bone tissue engineering. In this study, porous Si-doped tri-calcium phosphate (TCP) ceramics with a high porosity (~65%) and with interconnected macrotubes (~0.8mm in diameter) and micropores (5-100 μm) were prepared by firing hydroxyapatite (HA)/ bioactive glass-impregnated acrylontrile butadiene styrene (ABS) templates at 1400 °C. Results indicated that the cylindrical scaffolds had a higher compressive strength than the cubic scaffolds and the smallest cylindrical scaffold had a highest compressive strength (14.68+0.2MPa). Additional studies of cell attachment and MTT cytotoxicity assay proved the bioactivity and biocompatibility of the Si-doped TCP scaffolds.

scholarly journals Polydopamine-Assisted Surface Modification for Bone Biosubstitutes

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Shishu Huang ◽  
Nuanyi Liang ◽  
Yang Hu ◽  
Xin Zhou ◽  
Noureddine Abidi
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Coating Layer ◽  
Tissue Scaffolds ◽  
Surface Modifier ◽  
Biomaterial Surface ◽  
Superior Surface ◽  
Unique Surface

Polydopamine (PDA) prepared in the form of a layer of polymerized dopamine (DA) in a weak alkaline solution has been used as a versatile biomimetic surface modifier as well as a broadly used immobilizing macromolecule. This review mainly discusses the progress of biomaterial surface modification inspired by the participation of PDA in bone tissue engineering. A comparison between PDA-assisted coating techniques and traditional surface modification applied to bone tissue engineering is first presented. Secondly, the chemical composition and the underlying formation mechanism of PDA coating layer as a unique surface modifier are interpreted and discussed. Furthermore, several typical examples are provided to evidence the importance of PDA-assisted coating techniques in the construction of bone biosubstitutes and the improvement of material biocompatibility. Nowadays, the application of PDA as a superior surface modifier in multifunctional biomaterials is drawing tremendous interests in bone tissue scaffolds to promote the osteointegration for bone regeneration.

Preparation of Macroporous Glass-Ceramic Composites Containing β-TCP

2006 ◽  
Vol 11-12 ◽  
pp. 223-226
Author(s):  
Akiko Obata ◽  
Megumi Sasaki ◽  
Toshihiro Kasuga
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Glass Ceramic ◽  
Tricalcium Phosphate ◽  
Ceramic Composites ◽  
Calcium Pyrophosphate ◽  
Macroporous Glass ◽  
Glass Powders

A macroporous phosphate invert glass ceramic (PIGC) was prepared by dipping polymer sponges in the powder-slurry of the mother glass with a composition of 60CaO-30P2O-3TiO2- 7Na2O in mol%, and subsequent burning off the sponge at 850°C for 1 hr. The macroporous PIGC consists predominantly of ß-tricalcium phosphate (β-TCP) and ß-calcium pyrophosphate, and it has macropores of 500 μm in diameter and porosity of 83 %. Its compressive strength was estimated to be 160 kPa. The PIGC composite containing a large amount of β-TCP was also prepared by heating the mixture of Ca(OH)2 with the mother glass powders of the PIGC. Solubility of the composite was higher than PIGC. The macroporous PIGC and PIGC composite were expected to be applicable in high resorbable scaffolds for bone tissue engineering.

Preparation and characterization of calcium phosphate/pectin scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 62071-62082 ◽  
Author(s):  
Lisheng Zhao ◽  
Junjie Li ◽  
Liang Zhang ◽  
Yu Wang ◽  
Jiexin Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Clinical Application ◽  
Calcium Phosphate Cement ◽  
Bone Defects

A calcium phosphate cement (CPC) scaffold has been used to repair bone defects, but its low compressive strength and poor osteogenesis greatly hinder its clinical application.

Fabrication of a RP-Based Biomimetic Grafting Material for Bone Tissue Engineering

2009 ◽  
Vol 626-627 ◽  
pp. 553-558 ◽  
Author(s):  
Xing Ma ◽  
Y.Y. Hu ◽  
Xiao Ming Wu ◽  
J. Liu ◽  
Zhuo Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Autologous Bone Marrow ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
High Porosity ◽  
Highly Porous

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

scholarly journals Synthesis and Characterization of Poly(lactic-co-glycolic) Acid Nanoparticles-Loaded Chitosan/Bioactive Glass Scaffolds as a Localized Delivery System in the Bone Defects

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
K. Nazemi ◽  
F. Moztarzadeh ◽  
N. Jalali ◽  
S. Asgari ◽  
M. Mozafari
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Glycolic Acid ◽  
Plga Nanoparticles ◽  
Biological Macromolecules ◽  
High Porosity ◽  
Tissue Engineering Scaffolds ◽  
Localized Delivery

The functionality of tissue engineering scaffolds can be enhanced by localized delivery of appropriate biological macromolecules incorporated within biodegradable nanoparticles. In this research, chitosan/58S-bioactive glass (58S-BG) containing poly(lactic-co-glycolic) acid (PLGA) nanoparticles has been prepared and then characterized. The effects of further addition of 58S-BG on the structure of scaffolds have been investigated to optimize the characteristics of the scaffolds for bone tissue engineering applications. The results showed that the scaffolds had high porosity with open pores. It was also shown that the porosity decreased with increasing 58S-BG content. Furthermore, the PLGA nanoparticles were homogenously distributed within the scaffolds. According to the obtained results, the nanocomposites could be considered as highly bioactive bone tissue engineering scaffolds with the potential of localized delivery of biological macromolecules.

scholarly journals In VitroDegradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Naznin Sultana ◽  
Tareef Hayat Khan
Keyword(s):  
Tissue Engineering ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Initial Period ◽  
Tissue Engineering Application ◽  
High Porosity ◽  
Composite Scaffolds

This paper investigated the long-termin vitrodegradation properties of scaffolds based on biodegradable polymers and osteoconductive bioceramic/polymer composite materials for the application of bone tissue engineering. The three-dimensional porous scaffolds were fabricated using emulsion-freezing/freeze-drying technique using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) which is a natural biodegradable and biocompatible polymer. Nanosized hydroxyapatite (nHA) particles were successfully incorporated into the PHBV scaffolds to render the scaffolds osteoconductive. The PHBV and nHA/PHBV scaffolds were systematically evaluated using various techniques in terms of mechanical strength, porosity, porous morphology, andin vitrodegradation. PHBV and nHA/PHBV scaffolds degraded over time in phosphate-buffered saline at 37°C. PHBV polymer scaffolds exhibited slow molecular weight loss and weight loss in thein vitrophysiological environment. Accelerated weight loss was observed in nHA incorporated PHBV composite scaffolds. An increasing trend of crystallinity was observed during the initial period of degradation time. The compressive properties decreased more than 40% after 5-monthin vitrodegradation. Together with interconnected pores, high porosity, suitable mechanical properties, and slow degradation profile obtained from long-term degradation studies, the PHBV scaffolds and osteoconductive nHA/PHBV composite scaffolds showed promises for bone tissue engineering application.

Constructing a novel three-dimensional scaffold with mesoporous TiO2 nanotubes for potential bone tissue engineering

2015 ◽  
Vol 3 (27) ◽  
pp. 5595-5602 ◽  
Author(s):  
Yizao Wan ◽  
Peng Chang ◽  
Zhiwei Yang ◽  
Guangyao Xiong ◽  
Ping Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Tio2 Nanotubes ◽  
Sol Gel ◽  
Three Dimensional ◽  
Mesoporous Tio2 ◽  
Tissue Engineering Scaffold ◽  
Porous Network

A novel 3D porous network-structured tissue engineering scaffold built of mesoporous TiO2 nanotubes has been synthesized via the bacterial cellulose-templated sol–gel route followed by calcination.

Preparation and Properties on Silk Fibers Reinforced Hydroxyapatite/Chitosan Composites

2010 ◽  
Vol 105-106 ◽  
pp. 557-560 ◽  
Author(s):  
Jing Wang ◽  
Q.Z. Sun ◽  
Jing Gao ◽  
D.M. Liu ◽  
Xiang Cai Meng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Compressive Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biological Properties ◽  
X Rays ◽  
Silk Fibers ◽  
Initial Strength ◽  
Scaffold Materials

Silk fibers were introduced into hydroxyapatite(HA)/chitosan(CS) matrix to prepare scaffold materials of bone tissue engineering with the adequate initial strength and improved cellular affinity using combination of in situ synthesis and freeze-drying technique. Chemical component was investigated using X rays diffraction (XRD) and Fourier transform infrared spectrum (FTIR). Structure and morphology of the composites were observed by scanning electron microscope (SEM). Porosity was tested by liquid substitution method. The mechanical properties of the composites were also measured. The simulated body fluid (SBF) and the cell culture experiments were conducted to assess biological properties of the composites. Results show that the composites with a pore size of 100~250μm have a porosity of 75%~90%and the maximum compressive strength of 5.7 MPa. The compressive strength of the composite is greatly improved in comparison with that of HA/CS matrix (4.6 MPa). In the SBF tests, a layer of randomly oriented apatite crystals form on the scaffold surface after sample immersion in SBF. The cell culture experiments show that the osteoblast cells are attached and proliferated on the surface of the composite, which suggests good bioactivity and cellular compatibility of the composite material. It is concluded that the composites have a promising prospect as bone tissue engineering materials.

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