scholarly journals DCDR Spectroscopy as Efficient Tool for Liposome Studies: Aspect of Preparation Procedure Parameters

2012 ◽  
Vol 27 ◽  
pp. 349-353 ◽  
Author(s):  
E. Kočišová ◽  
A. Vodáková ◽  
M. Procházka
Keyword(s):  
Lipid Composition ◽  
Phosphate Buffer ◽  
Initial Phase ◽  
Liquid Crystalline ◽  
Phase State ◽  
Hydrophobic Surface ◽  
Drying Process ◽  
Lipid Concentration ◽  
Efficient Tool ◽  
Coating Deposition

Drop-coating deposition Raman (DCDR) spectroscopy was employed to study liposome suspensions. The method is based on a specific drying process on the hydrophobic surface that efficiently accumulates the macromolecular sample in a ring of the edge of the dried drop. We studied liposome suspensions purchased from two sources (Avanti Polar Lipids, Inc. and Sigma-Aldrich, Co.) and prepared under different conditions. Structure of the dried drop substantially depends on the lipid concentration, lipid composition of the sample, and used solvent. Optimal lipid concentration is about 0.3 mg/ml in all cases, asolectin and DSPC suspensions form compact dried drops when dissolved in water and phosphate buffer, respectively. Drying process of the sample drop does not influence the initial phase state (gel or liquid-crystalline) of the studied liposomes excepting DSPC from Sigma-Aldrich, Co.

scholarly journals Interaction of porphyrin/oligonucleotide complex with liposomes studied by drop coating deposition Raman spectroscopy

2010 ◽  
Vol 24 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
E. Kocišová ◽  
M. Procházka ◽  
J. Štepánek ◽  
P. Mojzeš
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Composition ◽  
Hydrophobic Surface ◽  
Small Species ◽  
Drying Process ◽  
High Quality ◽  
Coating Deposition ◽  
Solution Sample ◽  
Plasmatic Membrane ◽  
Edge Part

Drop coating deposition Raman (DCDR) microspectroscopy was used to investigate interaction of the complexed cationic copper 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuP) and phosphorothioate analog of dT15oligonucleotide with liposomes, the lipid composition of which imitated the natural plasmatic membrane. Great advantage of dried drops on DCDR plates over a solution sample is that the specific drying process on the special hydrophobic surface efficiently separates liposomes from small species in the solvent. In our case, liposomes with bound CuP/oligonucleotide complexes formed a ring at the edge part of the dried drop while dried solution of this complex remained inside this ring. High quality spectra measured from the ring by using Raman confocal microspectrometer revealed unperturbed arrangement of lipid chains by the drying process, partial binding of the CuP/oligonucleotide complexes to liposomes, and a certain reorientation of lipid chains as a consequence of this interaction.

scholarly journals Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

2021 ◽  
Vol 22 (4) ◽  
pp. 2191
Author(s):  
Jing Huang ◽  
Nairveen Ali ◽  
Elsie Quansah ◽  
Shuxia Guo ◽  
Michel Noutsias ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Outer Ring ◽  
Imaging Method ◽  
Fluorescence Lifetime Imaging ◽  
Drying Process ◽  
Vibrational Signal ◽  
Coating Deposition ◽  
Ft Ir ◽  
Sample Droplet ◽  
Ft Ir Spectroscopy

In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy.

Dependence of trehalose protective action on the initial phase state of dipalmitoylphosphatidylcholine bilayers

Cryobiology ◽  
1988 ◽  
Vol 25 (3) ◽  
pp. 256-263 ◽  
Author(s):  
N. Tsvetkova ◽  
B. Tenchov ◽  
L. Tsonev ◽  
Ts. Tsvetkov
Keyword(s):  
Initial Phase ◽  
Phase State ◽  
Protective Action

The effect of silver nanoparticles on the phase state of comb-shaped liquid crystalline polymers with cyanobiphenyl mesogenic groups

2006 ◽  
Vol 48 (7) ◽  
pp. 665-675 ◽  
Author(s):  
E. B. Barmatov ◽  
A. S. Medvedev ◽  
D. A. Pebalk ◽  
M. B. Barmatova ◽  
N. A. Nikonorova ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Liquid Crystalline ◽  
Phase State ◽  
Liquid Crystalline Polymers ◽  
Crystalline Polymers

Study of the effect of director fluctuations on liquid-crystalline molecular reorientation

2014 ◽  
Vol 22 (2) ◽  
Author(s):  
M. Sierakowski ◽  
T. Woliński ◽  
K. Orzechowski
Keyword(s):  
Initial Phase ◽  
Liquid Crystalline ◽  
Optical Excitation ◽  
Practical Aspect ◽  
Molecular Reorientation ◽  
Restricted Area ◽  
Phase Fluctuations ◽  
Stochastic Events ◽  
The Impact ◽  
Reorientation Process

AbstractInitial phase of field-induced molecular reorientation in threshold geometry of a liquid-crystalline structure is essentially influenced by thermal orientational fluctuations. Still not quite clear is the way in which the reorientation starts and how it runs over an LC-structure in its initial phase. Fluctuations are stochastic events in time and also in space. This feature suggests, that the reorientation process may be induced by director fluctuations locally in a particular part of the LC-structure. In this work we have studied the impact of fluctuations on the molecular reorientation in nematics involving optical nonlinear interaction. The restricted area of a nematic layer was excited by light beam that amplified director fluctuations locally. By manipulation of optical excitation we were able to affect the dynamics of the effect. Since the threshold-type configuration of nematics is used in a number of optical devices, the obtained results may have practical aspect contributing to a new way of controlling the field-induced transition.

Effect of Phosphate-Buffered Sucrose Flush Solution upon the Initial Phase of Ischaemic Acute Renal Failure in the Rat

1989 ◽  
Vol 77 (1) ◽  
pp. 77-84 ◽  
Author(s):  
O. S. Ferwana ◽  
S. C. Pirie ◽  
D. J. Potts
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Phosphate Buffer ◽  
Initial Phase ◽  
Protective Action ◽  
Control Group ◽  
Anaesthetized Rats

1. Experiments were performed upon anaesthetized rats to investigate the effect upon the initial 4 h of ischaemic acute renal failure of intrarenal flush with phosphate buffer (PB), sucrose alone and phosphate-buffered sucrose (PBS) solutions of differing compositions. 2. Kidneys flushed with PB immediately before 45 min pedicle clamp ischaemia exhibited a post-ischaemic function similar to that of control, non-flushed kidneys: they were isosthenuric and non-oliguric with a clearance of inulin (Cin) depressed to 10% of the pre-ischaemic value. 3. Kidneys flushed with 50 mmol/l sucrose in saline before ischaemia became isosthenuric, oliguric and had a more severely depressed post-ischaemic Cin compared with non-flushed kidneys. 4. Kidneys flushed with PBS containing either 50 (PBS50) or 140 (PBS140) mmol/l sucrose at pH 7 became polyuric after ischaemia, produced concentrated urine and had Cin values significantly higher than control kidneys. 5. Raising the pH of the PBS50 to 7.4 resulted in isosthenuric, polyuric kidneys that had a post-ischaemic Cin not significantly different from that of the control group. 6. It is concluded that the severity of the initial phase of ischaemic acute renal failure was significantly reduced by intrarenal flush with a PBS solution of pH 7; flush with PB or sucrose alone had no protective action.

Effect of the initial phase state of DT-matter on the compression of inertial fusion targets

2011 ◽  
Vol 32 (6) ◽  
pp. 596-603 ◽  
Author(s):  
Sergey Yu. Gus’kov ◽  
Nikolay V. Zmitrenko ◽  
Vladislav B. Rozanov
Keyword(s):  
Initial Phase ◽  
Phase State ◽  
Inertial Fusion

PEGylation of Phytantriol-Based Lyotropic Liquid Crystalline Particles—The Effect of Lipid Composition, PEG Chain Length, and Temperature on the Internal Nanostructure

Langmuir ◽  
2014 ◽  
Vol 30 (22) ◽  
pp. 6398-6407 ◽  
Author(s):  
Christa Nilsson ◽  
Jesper Østergaard ◽  
Susan Weng Larsen ◽  
Claus Larsen ◽  
Arto Urtti ◽  
...  
Keyword(s):  
Chain Length ◽  
Lipid Composition ◽  
Liquid Crystalline ◽  
Peg Chain Length

scholarly journals Development of Selection Indices for Improvement of Seed Yield and Lipid Composition in Bambara Groundnut (Vigna subterranea (L.) Verdc.)

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 86
Author(s):  
Razlin Azman Halimi ◽  
Carolyn A. Raymond ◽  
Bronwyn J. Barkla ◽  
Sean Mayes ◽  
Graham J. King
Keyword(s):  
Oleic Acid ◽  
Lipid Composition ◽  
Gene Pool ◽  
Dry Weight ◽  
Bambara Groundnut ◽  
Seed Lipid ◽  
Vigna Subterranea ◽  
Seed Number ◽  
Lipid Concentration ◽  
Selection Indices

The underutilised grain legume bambara groundnut (Vigna subterranea) has the potential to contribute significantly to nutritional security. However, the lack of commercial cultivars has hindered its wider adoption and utilisation as a food source. The development of competitive cultivars is impeded by (1) lack of systematic data describing variation in nutritional composition within the gene pool, and (2) a poor understanding of how concentrations of different nutritional components interact. In this study, we analysed seed lipid and protein concentration and lipid composition within a collection of 100 lines representing the global gene pool. Seed protein and lipid varied over twofold with a normal distribution, but no significant statistical correlation was detected between the two components. Seed lipid concentration (4.2–8.8 g/100 g) is primarily determined by the proportion of oleic acid (r2 = 0.45). Yield and composition data for a subset of 40 lines were then used to test selection parameters for high yielding, high lipid breeding lines. From five selection indices tested using 15 scenarios, an index based on the seed number, seed weight, and oleic acid yielded a > 50% expected increase in each of the mean values of seed number, pod dry weight, seed dry weight, and seed size, as well as an expected 7% increase in seed lipid concentration.

scholarly journals CITIZEN SCIENCE FOR WATER QUALITY MONITORING APPLYING FOSS

2019 ◽  
Vol XLII-4/W14 ◽  
pp. 119-126
Author(s):  
S. Jovanovic ◽  
D. Carrion ◽  
M. A. Brovelli
Keyword(s):  
Water Quality ◽  
Open Source ◽  
Citizen Science ◽  
Initial Phase ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Smartphone Applications ◽  
Efficient Tool ◽  
State Of Art

<p><strong>Abstract.</strong> Citizen science is an efficient tool for data collection for scientific projects. In the framework of SIMILE Interreg Italy-Switzerland project, the citizen’s contribution to monitor the quality of lakes water has been envisaged. In the initial phase of this research, state of art of citizen science and water quality monitoring was investigated. The analysis of past and current projects, governed by different organizations and communities, pointed out a variety of tasks that can be accomplished by citizens. In these studies, authors stressed suitability of smartphones for the fulfilment of various assignments given to citizens. Due to that, in this research, different smartphone applications for water quality monitoring were tested and analyzed. Despite the fact that many applications for water quality monitoring are freely available, none of them is open source. Hence, this paper is proposing the design of a new application, for the purposes of SIMILE project, that will be free and open source, addressing not just users but also developers giving them a possibility for customization and improvement.</p>

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