scholarly journals Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Isaac A. Rodriguez ◽  
Parthasarathy A. Madurantakam ◽  
Jennifer M. McCool ◽  
Scott A. Sell ◽  
Hu Yang ◽  
...  
Keyword(s):  
Donor Site ◽  
Donor Site Morbidity ◽  
Alizarin Red ◽  
Mineralization Potential ◽  
Before And After ◽  
Cleft Palate Repair ◽  
Palate Repair ◽  
Preliminary Study ◽  
Bone Autograft ◽  
Mineralized Fibers

The current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatite : fibrinogen (PDO : nHA : Fg) blended scaffolds in different simulated body fluids (SBF). Scaffolds were fabricated by blending PDO : nHA : Fg in the following percent by weight ratios: 100 : 0 : 0, 50 : 25 : 25, 50 : 50 : 0, 50 : 0 : 50, 0 : 0 : 100, and 0 : 50 : 50. Samples were immersed in (conventional (c), revised (r), ionic (i), and modified (m)) SBF for 5 and 14 days to induce mineralization. Scaffolds were characterized before and after mineralization via scanning electron microscopy, Alizarin Red-based assay, and modified burnout test. The addition of Fg resulted in scaffolds with smaller fiber diameters. Fg containing scaffolds also induced sheet-like mineralization while individual fiber mineralization was noticed in its absence. Mineralized electrospun Fg scaffolds without PDO were not mechanically stable after 5 days in SBF, but had superior mineralization capabilities which produced a thick bone-like mineral (BLM) layer throughout the scaffolds. 50 : 50 : 0 scaffolds incubated in either r-SBF for 5 days or c-SBF for 14 days produced scaffolds with high mineral content and individual-mineralized fibers. These mineralized scaffolds were still porous and will be further optimized as an effective bone substitute in future studies.

scholarly journals Analysis of the Pro- and Anti-Inflammatory Cytokines Secreted by Adult Stem Cells during Differentiation

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Amy L. Strong ◽  
Jeffrey M. Gimble ◽  
Bruce A. Bunnell
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Inflammatory Cytokines ◽  
Adult Stem Cells ◽  
Adipogenic Differentiation ◽  
Donor Site ◽  
Donor Site Morbidity ◽  
Alizarin Red ◽  
Anti Inflammatory

Adipose-derived stromal/stem cells (ASCs) are adult stem cells that have the potential to differentiate into mesenchymal lineage cells. The abundance of ASCs in adipose tissue and easy accessibility with relatively little donor site morbidity make them attractive candidate cells for tissue engineering and regenerative medicine. However, the underlying inflammatory process that occurs during ASC differentiation into adipocytes and osteoblast has not been extensively investigated. ASCs cultured in osteogenic and adipogenic differentiation medium were characterized by oil red o staining and alizarin red staining, respectively. ASCs undergoing osteogenic and adipogenic differentiation were isolated on days 7, 14, and 21 and assessed by qRT-PCR for the expression of pro- and anti-inflammatory cytokines. ASCs undergoing osteogenic differentiation expressed a distinct panel of cytokines that differed from the cytokine profile of ASCs undergoing adipogenic differentiation at each of the time points analyzed. Mapping the cytokine expression profile during ASC differentiation will provide insight into the role of inflammation in this process and identify potential targets that may aid in enhancing osteogenic or adipogenic differentiation for the purposes of tissue engineering and regenerative medicine.

scholarly journals Outcome of Anterior Cervical Discectomy and Fusion for Cervical Spondylotic Myelopathy

2019 ◽  
Vol 9 (2) ◽  
pp. 133-137
Author(s):  
Apel Chandra Saha ◽  
Md Hasan Masud
Keyword(s):  
Cervical Spondylotic Myelopathy ◽  
Cervical Spinal Cord ◽  
Donor Site ◽  
Fusion Rate ◽  
Donor Site Morbidity ◽  
Anterior Cervical Discectomy ◽  
Common Procedure ◽  
Cervical Discectomy ◽  
Operative Complications ◽  
Before And After

Background: Cervical spondylotic myelopathy (CSM) is a progressive degenerative disease and the most common cause of cervical spinal cord dysfunction (SCD) in older patients. Anterior cervical discectomy andfusion (ACDF) is a common procedure for patients with severe neurological deterioration. The goals of this study were to evaluate the clinical and functional outcome, radiological fusion and operative complications in case of CSM who underwent ACDF by autogeneous-tricortical bone graft and stabilized with plate and screws. Methods: This prospective interventional study was carried out at National Institute of Traumatology and Orthopaedic Rehabilitation ( NITOR) and different private hospitals in Dhaka from January 2012 to December 2014. Within this period total 12 CSM patients were included as study sample. All were surgically treated by ACDF and stabilized by plate and screws. All patients were clinically and radiologically evaluated before and after surgery. Results: Single level ACDF by autograft and stabilization by plate and screws was done in 10 (83.33%) patients and 2 (16.67%) patients had two level fusion. The mean follow up period was 12 months. The satisfactory result was found in 10 (83.33%) patients. Post-operative complications were donor site morbidity in2 (16.67%) patients and transient dysphagia in 1 (8.33%) patient. The fusion rate was 100% in this series. Conclusion: ACDF with anterior plating for CSM is a safe and effective procedure. It results in highest fusion, least complication and relatively lower cost. Birdem Med J 2019; 9(2): 133-137

scholarly journals Stair Ascent and Descent in Assessing Donor-Site Morbidity Following Osteocutaneous Free Fibula Transfer: A Preliminary Study

2015 ◽  
Vol 73 (1) ◽  
pp. 184-193 ◽  
Author(s):  
Alessandro Baj ◽  
Nicola Lovecchio ◽  
Alessandro Bolzoni ◽  
Andrea Mapelli ◽  
Aldo Bruno Giannì ◽  
...  
Keyword(s):  
Donor Site ◽  
Donor Site Morbidity ◽  
Stair Ascent ◽  
Free Fibula ◽  
Preliminary Study

Gait performance in assessing donor-site morbidity following osteocutaneous free fibula transfer: a preliminary study

2017 ◽  
Vol 57 ◽  
pp. 212
Author(s):  
Ilaria Pacifici ◽  
Ludovica Pallotta ◽  
Alessandro Bolzoni ◽  
Giada Beltramini ◽  
Matteo Zago ◽  
...  
Keyword(s):  
Donor Site ◽  
Donor Site Morbidity ◽  
Gait Performance ◽  
Free Fibula ◽  
Preliminary Study

scholarly journals Radial vs ulnar forearm flap: a preliminary study of donor site morbidity

2019 ◽  
Vol 39 (5) ◽  
pp. 322-328
Author(s):  
G. Bertino ◽  
Y. Lepenne ◽  
C. Tinelli ◽  
L. Giordano ◽  
S. Cacciola ◽  
...  
Keyword(s):  
Donor Site ◽  
Donor Site Morbidity ◽  
Forearm Flap ◽  
Preliminary Study
Sign in / Sign up

Export Citation Format

Share Document