scholarly journals Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
P. Saravanan ◽  
R. Muthuvelayudham ◽  
T. Viruthagiri
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Activity ◽  
Statistical Design ◽  
Cellulase Production ◽  
Positive Influence ◽  
Mango Peel ◽  
Soybean Cake ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Cake Flour

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.

scholarly journals Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
P. Saravanan ◽  
R. Muthuvelayudham ◽  
T. Viruthagiri
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Pineapple Waste ◽  
Enhanced Production ◽  
Validation Experiment ◽  
Soybean Cake ◽  
Cake Flour

Optimization of the media components for cellulase production usingTrichoderma reeseiwas carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence on the cellulase production was studied using a Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and yeast extract were found to have the positive influence for the production of cellulase. The selected components were optimized using response surface methodology. The optimum concentrations are avicel: 26.5 g/L, soybean cake flour: 22.5 g/L, KH2PO4: 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment.

Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

2012 ◽  
Vol 518-523 ◽  
pp. 5578-5585
Author(s):  
Nan Ban ◽  
Yu Jie Zhou ◽  
Yan Ping Ye ◽  
Lin Mei Dai ◽  
Alatangaole Damirn ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Penicillium Decumbens ◽  
Initial Ph ◽  
Effects Of Ph ◽  
Burman Design ◽  
The Stability ◽  
Plackett Burman Design

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

scholarly journals Enhanced Saccharfication of Pomegranate Peel by Trichoderma Reesei and Statiscal Optimization of Medium Composition by Response Surface Methodology

2021 ◽  
Author(s):  
P.SARAVANAN PANCHAMOORTHY ◽  
R.Rajesh Kannan
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Medium Composition ◽  
Central Component ◽  
Pomegranate Peel ◽  
Component Design ◽  
Media Component ◽  
Soybean Cake ◽  
The Media ◽  
Burman Design

Abstract In this work, pomegranate peels (PPs) otherwise considered as waste is used as substrates for producing cellulase. Pomegranate peel was employed as a carbon source to be produced of cellulase in submerged state fermentation (SmF). Statistical experimental designs were employed to screen the nutrients and optimize the media composition for cellulase production by Trichoderma reesei. Nine nutrients were selected by the preliminary screening of medium component production by plackett –burman design (PBD) technique. Four nutrients have been found to be important for cellulase production and optimized by central component design (CCD). The best media component for submerged fermentation of cellulase using pomegranate peel were avicel– 24.812 g/l, KH2PO4 – 4.626 g/l, soybean cake flour – 20.7 g/l and MnSO4.H2O-1.036 g/l. Under these optimum conditions, the production of cellulase was found to be 9.3 IU/mL. The optimum factors acquired from the statistical model were further confirmed using the experimental results.

scholarly journals Enhanced Cellulase Production by Talaromyces Amestolkiae CMIAT055 using Banana Pseudostem

2021 ◽  
Author(s):  
Genilton S Faheina ◽  
Kally A Sousa ◽  
Jerri E Zilli ◽  
Carlos Vergara ◽  
Gustavo A. Saavedra Pinto ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Strain ◽  
Cellulase Activity ◽  
Statistical Design ◽  
Ph Control ◽  
Cellulase Production ◽  
Statistical Experimental Design ◽  
Natural Carbon ◽  
The Cost ◽  
Hydrolysis Of

Abstract Cellulases are a complex of enzymes necessary for the complete solubilization of cellulose in sugars, thus playing a key role in the natural carbon cycle through the hydrolysis of lignocellulosic structures. The aim of this study was to evaluate the increase in the capacity of Talaromyces amestolkiae CMIAT 055 to produce cellulases by optimizing the components of the culture medium containing banana pseudostem as an inducer, as well as in different agitation configurations in a bioreactor. Optimization was performed through statistical experimental design (Plackett-Burman and DCCR), a study of pH control in bioreactors, and a study of the agitation system by comparing impellers with different flow profiles in the liquid medium. For this purpose, a wild strain of Talaromyces amestolkiae CMIAT 055 was used. In the Plackett-Burman and DCCR statistical design, four components of the culture medium were significant and optimized for greater synthesis of FPase: banana pseudostem, CaCl2, KH2PO4, and urea. In bioreactors tests, these parameters were beneficial for greater enzyme activities: maintenance of pH at 5.0, use of Pitched blade impeller, and rotation speed at 300 rpm. Comparing the first test using banana pseudostem in an Erlenmeyer flask to the last fermentation process in bioreactors, it was observed that the total cellulase activity increased from 424.7 FPU/L to 2172.8 FPU/L. This fact showed that the strategies adopted in this study are a pertinent way to reduce the cost of enzyme production through the use of lignocellulosic materials.

scholarly journals Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch

2018 ◽  
Vol 13 (3) ◽  
pp. 553
Author(s):  
Vita Wonoputri ◽  
Subiantoro Subiantoro ◽  
Made Tri Ari Penia Kresnowati ◽  
Ronny Purwadi
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Contact Method ◽  
Empty Fruit Bunch ◽  
Liquid Ratio ◽  
Fermentation Parameters

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reservedReceived: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559 

scholarly journals Optimization of inulinase production using copra waste by K. marxianus var. marxianus

2010 ◽  
Vol 16 (4) ◽  
pp. 319-327 ◽  
Author(s):  
M. Dilipkumar ◽  
M. Rajasimman ◽  
N. Rajamohan
Keyword(s):  
Response Surface Methodology ◽  
Carbon Source ◽  
Corn Steep Liquor ◽  
Positive Influence ◽  
Experimental Designs ◽  
Statistical Methodology ◽  
Optimum Conditions ◽  
Burman Design ◽  
Steep Liquor ◽  
Plackett Burman Design

Kluyveromyces marxianus var marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett-Burman design. Corn steep liquor, (NH4)2SO4, ZnSO4.7H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: corn steep liquor - 0.0560 (g/gds), (NH4)2SO4 - 0.0084 (g/gds), ZnSO4.7H2O - 0.0254 (g/gds), K2HPO4 - 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.

scholarly journals Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

2021 ◽  
Author(s):  
Marwa El-Sesy ◽  
Amira M Aly
Keyword(s):  
Rice Straw ◽  
Enzyme Production ◽  
Paper Industry ◽  
Cellulase Production ◽  
Barley Straw ◽  
Cellulase Enzyme ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Aspergilus Niger ◽  
Industry Wastewater

Abstract A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.

scholarly journals Response surface optimization of medium composition for xylitol production by Debaryomyces hansenii var hansenii using corncob hemicellulose hydrolysate

2013 ◽  
Vol 19 (3) ◽  
pp. 377-384 ◽  
Author(s):  
S. Ramesh ◽  
R. Muthuvelayudham ◽  
Rajesh Kannan ◽  
T. Viruthagiri
Keyword(s):  
Response Surface ◽  
Yeast Extract ◽  
Debaryomyces Hansenii ◽  
Positive Influence ◽  
Medium Composition ◽  
Xylitol Production ◽  
Hemicellulose Hydrolysate ◽  
Response Surface Optimization ◽  
Burman Design ◽  
Plackett Burman Design

Optimization of the culture medium for xylitol production using Debrayomyces hansenii var hansenii was carried out. The optimization of xylitol production using corncob hemicelluloses hydrolysate as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence of xylitol production to achieved using a Plackett-Burman design. MgSO4.7H2O, KH2PO4, (NH4)2SO4, yeast extract were selected for based on their positive influence on xylitol production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: MgSO4.7H2O - 1.02 g/l, (NH4)2SO4 - 3.94 g/l, KH2PO4- 2.74 g/l and yeast extract - 3.45 g/l. These conditions are validated experimentally which revealed an enhanced xylitol yield of 0.76 g/g.

scholarly journals Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design

2011 ◽  
Vol 10 (46) ◽  
pp. 9402-9409 ◽  
Author(s):  
Zauri Abdul Wahid Manisya ◽  
Salleh Madihah ◽  
Yusof Faridah ◽  
Ismail Abdul Karim Mohammed ◽  
Zahangir Alam Md
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Oil Palm ◽  
Factors Affecting ◽  
Empty Fruit Bunches ◽  
Burman Design ◽  
C 30 ◽  
Plackett Burman Design

scholarly journals Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Dia Septiani ◽  
HERMAN SURYADI ◽  
Abdul Mun'im ◽  
WIBOWO MANGUNWARDOYO
Keyword(s):  
Aspergillus Niger ◽  
Ammonium Sulfate ◽  
Mixed Culture ◽  
Trichoderma Reesei ◽  
Crude Extract ◽  
Hydrolytic Enzymes ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Partial Purification ◽  
Cellulose Depolymerization

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

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