scholarly journals Evaluation of Lercanidipine in Paclitaxel-Induced Neuropathic Pain Model in Rat: A Preliminary Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Lekha Saha ◽  
Debasish Hota ◽  
Amitava Chakrabarti
Keyword(s):  
Neuropathic Pain ◽  
Pain Threshold ◽  
Antinociceptive Effect ◽  
Tail Flick ◽  
Cold Allodynia ◽  
Pain Model ◽  
Neuropathic Pain Model ◽  
Preliminary Study

Objective. To demonstrate the antinociceptive effect of lercanidipine in paclitaxel-induced neuropathy model in rat. Materials and Methods. A total of 30 rats were divided into five groups of six rats in each group as follows: Gr I: 0.9% NaCl, Gr II: paclitaxel + 0.9% NaCl, Gr III: paclitaxel + lercanidipine 0.5 μg/kg, Gr IV: paclitaxel + lercanidipine 1 μg/kg, and Gr V: paclitaxel + lercanidipine 2.5 μg/kg. Paclitaxel-induced neuropathic pain in rat was produced by single intraperitoneal (i.p.) injection of 1 mg/kg of paclitaxel on four alternate days (0, 2, 4, and 6). The tail flick and cold allodynia methods were used for assessing the pain threshold, and the assessments were done on days 0 (before first dose of paclitaxel) and on days 7, 14, 21, and 28. Results. There was a significant decrease (P<0.001) in the tail flick and cold allodynia latency in the paclitaxel-alone group from day 14 onward when compared with day 0. In the lercanidipine groups, the decrease in the tail flick and cold allodynia latency was not observed in 1.0 and 2.5 μg/kg groups and it was statistically significant (P<0.01) when compared with paclitaxel-alone group.

scholarly journals The activation of galanin receptor 2 plays an antinociceptive effect in nucleus accumbens of rats with neuropathic pain

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Yan Dong ◽  
Chong-Yang Li ◽  
Xiao-Min Zhang ◽  
Ya-Nan Liu ◽  
Shuang Yang ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nucleus Accumbens ◽  
Pain Threshold ◽  
Antinociceptive Effect ◽  
Galanin Receptor ◽  
Pain Model ◽  
Withdrawal Threshold ◽  
Neuropathic Pain Model ◽  
Galanin Receptors ◽  
Intact Rats

AbstractOur previous research has shown that galanin plays an antinociceptive effect via binding to galanin receptors (GalRs) in nucleus accumbens (NAc). This study focused on the involvement of GalR2 in galanin-induced antinociceptive effect in NAc of neuropathic pain rats. The chronic constriction injury of sciatic nerve (CCI) was used to mimic neuropathic pain model. The hind paw withdrawal latency (HWL) to thermal stimulation and hind paw withdrawal threshold (HWT) to mechanical stimulation were measured as the indicators of pain threshold. The results showed that 14 and 28 days after CCI, the expression of GalR2 was up-regulated in bilateral NAc of rats, and intra-NAc injection of GalR2 antagonist M871 reversed galanin-induced increases in HWL and HWT of CCI rats. Furthermore, intra-NAc injection of GalR2 agonist M1145 induced increases in HWL and HWT at day 14 and day 28 after CCI, which could also be reversed by M871. Finally, we found that M1145-induced antinociceptive effect in NAc of CCI rats was stronger than that in intact rats. These results imply that the GalR2 is activated in the NAc from day 14 to day 28 after CCI and GalR2 is involved in the galanin-induced antinociceptive effect in NAc of CCI rats.

Antinociceptive effect ofButea monospermaon vincristine-induced neuropathic pain model in rats

2012 ◽  
Vol 29 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Venkata Rathina Kumar Thiagarajan ◽  
Palanichamy Shanmugam ◽  
Uma Maheswari Krishnan ◽  
Arunachalam Muthuraman ◽  
Nirmal Singh
Keyword(s):  
Neuropathic Pain ◽  
Antinociceptive Effect ◽  
Pain Model ◽  
Neuropathic Pain Model

Antinociceptive effect of matrine on vincristine-induced neuropathic pain model in mice

2013 ◽  
Vol 35 (6) ◽  
pp. 815-821 ◽  
Author(s):  
Dun Linglu ◽  
Li Yuxiang ◽  
Xu Yaqiong ◽  
Zhou Ru ◽  
Ma Lin ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Antinociceptive Effect ◽  
Pain Model ◽  
Neuropathic Pain Model

scholarly journals Effect of Extract and Synthesized Derivatives of Isolated Compound from Symplocos chinensis f. Pilosa Ohwi on Neuropathic Pain in Mice

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1639
Author(s):  
Hyun-Yong Kim ◽  
Soo-Hyun Park ◽  
Guanglei Zuo ◽  
Kang Hyuk Kim ◽  
Seung Hwan Hwang ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Ames Test ◽  
Antinociceptive Effect ◽  
Water Extract ◽  
Tail Flick ◽  
Pain Model ◽  
Water Fraction ◽  
Antinociceptive Effects ◽  
Isolated Compound ◽  
Derivatives Of

Neuropathic pain is described as the “most terrible of all tortures that a nerve wound may inflict.” The aim of the present study was to demonstrate the antinociceptive effect of Symplocos chinensis f. pilosa Ohwi water extract (SCW) and synthesized derivatives of the isolated compound. The antinociceptive effect was tested using the acetic acid-induced writhing and 5% formalin tests. Antinociceptive effects on neuropathic pain were evaluated using the von Frey test with chronic constriction injury (CCI) and surgical nerve injury (SNI) models and tail-flick test with a vincristine-induced pain model. An Ames test was also conducted. 5-hydroxymethylfurfural (5-HMF) was isolated and derivatives were synthesized with various acid groups. Among the plant water extracts, SCW showed significantly effective activity. Additionally, SCW presented antinociceptive effects in the neuropathic pain models. The SCW water fraction resulted in fewer writhes than the other fractions, and isolated 5-HMF was identified as an effective compound. Because 5-HMF revealed a positive response in the Ames test, derivatives were synthesized. Among the synthesized derivations, 5-succinoxymethylfurfural (5-SMF) showed the best effect in the neuropathic pain model. Our data suggest that SCW and the synthesized compound, 5-SMF, possess effective antinociceptive activity against neuropathic pain.

scholarly journals PENGUKURAN TINGKAH LAKU PADA MODEL NYERI NEUROPATIK PERIFER: TIKUS DENGAN CCI (CHRONIC CONSTRICTION INJURY)

2018 ◽  
Vol 35 (3) ◽  
Author(s):  
Hanik Badriyah Hidayati ◽  
Paulus Sugianto ◽  
Junaidi Khotib ◽  
Chrismawan Ardianto ◽  
Kuntoro Kuntoro ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Pain Threshold ◽  
Chronic Constriction Injury ◽  
Peripheral Neuropathic Pain ◽  
Pain Model ◽  
Threshold Test ◽  
Injury Model ◽  
Withdrawal Behavior ◽  
Neuropathic Pain Model ◽  
Chronic Constriction Injury Model

BEHAVIORAL ASSESMENT OF THE PERIPHERAL NEUROPATHIC PAIN MODEL: RAT WITH CCI (CHRONIC CONSTRICTION INJURY)ABSTRACTNeuropathic pain occurs in 20% of patients with chronic pain. This mainly due to the lack of effective treatment and the presence of drug’s side effects. Animal models have been broadly utilized in validating the therapeutic target and the development of analgesic drugs. Chronic constriction injury (CCI) is a model of peripheral neuropathic pain characterized by allodynia and hyperalgesia. Chronic constriction injury model is produced by loose ligation of the sciatic nerve resulting in nerve damage, thus sensitizes and lowers pain threshold. The pain threshold test is performed by providing a pressure and thermal sensory stimulus that results in observable withdrawal behavior. This article discusses several methods in assessing pain-related behavior on the CCI neuropathic pain model.Keywords: Behavioral, chronic constriction injury, mechanical, neuropathic painABSTRAKNyeri neuropatik terjadi pada seperlima pasien dengan nyeri kronik. Hal ini disebabkan karena terapi nyeri neuropatik masih kurang efektif dan adanya efek samping obat. Model hewan terbukti bermanfaat untuk validasi target dan perkembangan obat analgesik. Chronic constriction injury (CCI) merupakan model nyeri neuropatik perifer dengan karakteristik alodinia dan hiperalgesia. Model CCI dibuat dengan memberikan ligasi longgar pada saraf skiatik yang menyebabkan kerusakan saraf, sehingga terjadi sensitisasi dan ambang nyeri menurun. Tes ambang nyeri dilakukan dengan memberikan stimulus sensorik berupa tekanan dan panas yang akan menghasilkan tingkah laku withdrawal yang bisa diobservasi. Artikel ini membahas penilaian tingkah laku nyeri pada model nyeri neuropatik CCI.Kata kunci: Chronic constriction injury, mekanik, nyeri neuropatik, tingkah laku

scholarly journals Antinociceptive Effect of Magnolol in a Neuropathic Pain Model of Mouse

2021 ◽  
Vol Volume 14 ◽  
pp. 2083-2093
Author(s):  
Xiao Zhang ◽  
Juntao Wang ◽  
Aihua Sui ◽  
Nannan Zhang ◽  
Qiulan Lv ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Antinociceptive Effect ◽  
Pain Model ◽  
Neuropathic Pain Model

In Vivo Biocompatibility of Alginate-PLL Microcapsules with Chromaffin Cells for the Alleviation of Chronic Pain

2005 ◽  
Vol 277-279 ◽  
pp. 62-66 ◽  
Author(s):  
Yu Mi Kim ◽  
Young Hoon Jeon ◽  
Gwang Chun Jin ◽  
Jeong Ok Lim ◽  
Woon Yi Baek
Keyword(s):  
Chronic Pain ◽  
Neuropathic Pain ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Cold Allodynia ◽  
Pain Model ◽  
Prominent Symptom ◽  
Neuropathic Pain Model ◽  
Adrenal Chromaffin

Intrathecal implants of adrenal medullary chromaffin cells relieve chronic pain by secreting catecholamines, opioids and other neuroactive substances. Recently, macrocapsules with hollow fibers were employed to isolate immunologically xenogeneic chromaffin cells, but the poor viability in vivo of the encapsulated chromaffin cells limited the usefulness of this method. In this study, we used microencapsulation technology to increase the viability of chromaffin cells. Bovine adrenal chromaffin cells were microencapsulated with alginate and poly-L-lysine and implanted intrathecally in a rat using the neuropathic pain model. Intrathecal implants of microencapsulated cells relieved cold allodynia, which is the most prominent symptom of the neuropathic pain model in a rat. Furthermore, the microencapsulated chromaffin cells were morphologically normal and retained their functionality. These findings suggest that the intrathecal implant of microencapsulated chromaffin cells might be a useful method for treating chronic pain.

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