scholarly journals Enterococcus faeciumWB2000 Inhibits Biofilm Formation by Oral Cariogenic Streptococci

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Nao Suzuki ◽  
Masahiro Yoneda ◽  
Yuko Hatano ◽  
Tomoyuki Iwamoto ◽  
Yosuke Masuo ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Mutans Streptococci ◽  
Streptococcus Sobrinus ◽  
Dual Cultures

This study investigated the inhibitory effect of probioticEnterococcus faeciumWB2000 on biofilm formation by cariogenic streptococci. The ability ofE. faeciumWB2000 and JCM5804 andEnterococcus faecalisJCM5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. TheEnterococcalstrains inhibited biofilm formation in dual cultures with the mutans streptococcal strainsStreptococcus mutansXc andStreptococcus sobrinusJCM5176 (P<0.05), but not with the noncariogenic streptococcal strains.Enterococcus faeciumWB2000 inhibited biofilm formation by 90.0% (9/10) of the clinicalS. mutansstrains and 100% (3/3) of the clinicalS. sobrinusstrains. After culturing, the pH did not differ between single and dual cultures. The viable counts of floating mutans streptococci were lower in dual cultures withE. faeciumWB2000 than in single cultures.Enterococcus faeciumWB2000 acted as a probiotic bacterial inhibitor of cariogenic streptococcal biofilm formation.

The inhibitory effect of Plectranthus barbatus and Plectranthus ecklonii leaves on the viability, glucosyltransferase activity and biofilm formation of Streptococcus sobrinus and Streptococcus mutans

2010 ◽  
Vol 119 (2) ◽  
pp. 664-668 ◽  
Author(s):  
Neusa L. Figueiredo ◽  
Sara Raquel M.M. de Aguiar ◽  
Pedro Luis Falé ◽  
Lia Ascensão ◽  
Maria Luisa M. Serralheiro ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Streptococcus Sobrinus

scholarly journals Susceptibility to Enterocins and Lantibiotic Bacteriocins of Biofilm-Forming Enterococci Isolated from Slovak Fermented Meat Products Available on the Market

2020 ◽  
Vol 17 (24) ◽  
pp. 9586
Author(s):  
Andrea Lauková ◽  
Anna Kandričáková ◽  
Eva Bino
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Food Industry ◽  
Meat Products ◽  
Low Grade ◽  
Enterococcus Hirae ◽  
Conventional Antibiotics ◽  
Enterocin A

This study investigated eight types of Slovak dry fermented meat products (salami and sausages) that are available on the market and were produced by three different producers in different regions of Slovakia. The total counts of enterococci in these products ranged from 2.0 up to 6.0 cfu/g (log10). Three species were identified among the 15 selected enterococcal strains; Enterococcus faecium (8 strains), Enterococcus faecalis (3) and Enterococcus hirae (4). They were hemolysis-negative (γ-hemolysis) with a biofilm-forming ability, which was evaluated as low-grade biofilm formation, susceptible to conventional antibiotics and mainly susceptible to lantibiotic bacteriocins, namely, gallidermin and nisin; they even showed a higher susceptibility to gallidermin than to nisin. They were also susceptible to enterocin–durancin, but most strains showed resistance to enterocin A/P. This study indicated that bacteriocins can play a key role in preventing and/or protecting from undesirable bacterial multiplication or contamination in the food industry and that they have great potential for further experimental applications.

scholarly journals Importance of Enterococcus spp. for Forming a Biofilm

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S354-S356 ◽  
Author(s):  
L. Necidová ◽  
B. Janštová ◽  
S. Karpíšková ◽  
Š. Cupáková ◽  
M. Dušková ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Dairy Products ◽  
Formation Potential ◽  
Farm Level ◽  
Milk Samples ◽  
Bulk Milk ◽  
Dairy Plant ◽  
Plant Level

The aim of this study was to monitore the capability of <I>Enterococcus fecalis</I> and <I>Enterococcus fecium</I> to form biofilms. Enterococci isolates originated from individual milk, bulk milk samples and environmental swabs obtained at farm level, dairy plant level including semi and final dairy products. Biofilm formation potential was determined by growing the tested strains in glas tubes containing BHI medium. The capability of forming biofilms was detected in 28% of <I>Enterococcus</I> spp. strains. Higher number of biofilm forming strains of the <I>Enterococcus faecium</I> (33%) than <I>Enterococcus faecalis</I> (28%) has been registered. Isolates obtained at plant level were forming biofilms more often than isolates from plant level and in final products (cheese and curd cheese), no isolate has been seen to be able to form biofilm.

scholarly journals Effects of Caries Activity on Compositions of Mutans Streptococci in Saliva-Induced Biofilm Formed on Bracket Materials

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4764
Author(s):  
Bum-Soon Lim ◽  
Bo-Hyun Kim ◽  
Won-Jun Shon ◽  
Sug-Joon Ahn
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Mutans ◽  
Mutans Streptococci ◽  
Growth Media ◽  
Streptococcus Sobrinus ◽  
Chain Reaction ◽  
Caries Activity ◽  
Material Type ◽  
Polymerase Chain ◽  
Total Bacteria

This study aimed to investigate effects of caries activity on composition of mutans streptococci in saliva-induced biofilms formed on bracket materials. Three bracket materials were used as specimens: ceramic, metal, and plastic. After saliva was collected using a spitting method from caries-active (CA, decayed, missing, filled teeth (DMFT) score ≥ 10) and caries-free (CF, DMFT score = 0) subjects, saliva was mixed with growth media in a proportion of 1:10. The saliva solution was then incubated with each bracket material. After a saliva-induced biofilm was developed on the surface of the bracket material, the amounts of total bacteria and mutans streptococci were determined using real-time polymerase chain reaction. The results showed that biofilms from CA saliva contained more mutans streptococci but less total bacteria than biofilms from CF saliva, regardless of material type. Adhesion of total bacteria to ceramic was higher than to plastic, regardless of caries activity. Mutans streptococci adhered more to ceramic than to metal and plastic in both biofilms from CA and CF saliva, but there was a difference in adhesion between Streptococcus mutans and Streptococcus sobrinus. The amount of S. mutans was higher than that of S. sobrinus in biofilms from CA saliva despite similar amounts of the two strains in biofilms from CF saliva. The stronger adhesion of S. mutans to ceramic than to metal and plastic was more evident in biofilms from CA saliva than in biofilms from CF saliva. This study suggests that caries activity and material type significantly influenced composition of mutans streptococci in biofilms formed on bracket materials.

Anti-Biofilm Formation of Streptococcus mutans by Jasmine Mouthwash

2018 ◽  
Vol 773 ◽  
pp. 323-327
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Oral Streptococci ◽  
Drug Of Choice ◽  
Causative Microorganism ◽  
Warm Temperate

Streptococcus mutans has been reported to be a major causative microorganism for oral biofilm associated with dental caries. Jasmine sambac or Arabian jasmine is a species of jasmine native to tropical and warm temperate regions particularly West and Southeast Asia. The antimicrobial activities of essential oil extracted from the flowers of J. sambac have been shown to attract researchers. Objective: To determine the anti-biofilm formation of S. mutans by mouthwash containing jasmine oil. Materials and Methods: S. mutans KPSK2, the cariogenic strain of oral streptococci was used in the study. The 24-h biofilms of S. mutans were formed on polystyrene plates treated with jasmine mouthwash. The 0.2% chlorhexidine gluconate and phosphate buffer saline mouthwash were used as a positive and negative control respectively. The amount of biofilm was quantified by crystal violet staining and spectrophotometry at an optical density of 595 nm. Results: Jasmine mouthwash showed a significant inhibitory effect on S. mutans biofilm formation by decreasing 43% of biofilm whereas that of chlorhexidine showed 71% reduction. Conclusion: The anti-biofilm formation property of jasmine mouthwash was elucidated; therefore it might be another drug of choice that can be used as an adjunct to control the oral health in the prevention of dental caries.

scholarly journals Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jorge Jesús Veloz ◽  
Nicolás Saavedra ◽  
Alexis Lillo ◽  
Marysol Alvear ◽  
Leticia Barrientos ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Antibiofilm Activity ◽  
Total Polyphenols

The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect onStreptococcus mutansgrowth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity onStreptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect onStreptococcus mutansand the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.

scholarly journals Low Xylitol Concentration induces Ultrastructural Cellular Changes in Streptococcus mutans and Streptococcus sobrinus

2015 ◽  
Vol 4 (1) ◽  
pp. 1-3
Author(s):  
Agnes de Fátima Faustino Pereira ◽  
Thiago Cruvinel da Silva ◽  
Maria Aparecida de Andrade Moreira Machado ◽  
Marília Afonso Rabelo Buzalaf
Keyword(s):  
Streptococcus Mutans ◽  
Ruthenium Red ◽  
Mutans Streptococci ◽  
Cellular Damage ◽  
Bacterial Cells ◽  
Streptococcus Sobrinus ◽  
Cellular Changes ◽  
Transmission Electron ◽  
Cellular Wall ◽  
Polysaccharide Capsules

ABSTRACT Background Xylitol is a sugar alcohol frequently used in the prevention of dental caries. However, the mechanism of action of xylitol on mutans streptococci is still unclear. This study evaluated the ultrastructural cellular changes in Streptococcus mutans and Streptococcus sobrinus after xylitol treatment. Materials and methods S. mutans ATCC 25175 and S. sobrinus ATCC 33478 were grown separately in BHI medium plus 0.2% sucrose at 37°C under microaerophilic conditions overnight. Then, planktonic cells were harvested by centrifugation, resuspended in a fresh medium and incubated with either 0.2% glucose or 0.2% xylitol solutions for 2 hours. To prepare bacterial cells for the evaluation of morphology of cellular walls and polysaccharide capsules, cultures were subsequently fixed with 2.5% glutaraldehyde, stained with ruthenium red, post-fixed with 1% osmium tetroxide, dehydrated in acetone, embedded in Epon LX112 and analyzed by a transmission electron microscopy. Results Bacterial cells incubated with glucose showed welldefined cellular walls and well-stained cytoplasmic membrane. Unlike, xylitol yielded diffuse cellular wall and irregular polysaccharide capsules in both microorganisms. Conclusion Low xylitol concentrations also can induce cellular damage to both S. mutans and S. sobrinus. Further studies are needed to provide more evidence on the anticariogenic effect of xylitol. How to cite this article Pereira AFF, Silva TC, Machado MAAM, Buzalaf MAR. Low Xylitol Concentration induces Ultrastructural Cellular Changes in Streptococcus mutans and Streptococcus sobrinus. Int J Experiment Dent Sci 2015;4(1):1-3.

scholarly journals Inhibitory Effect ofBacillus velezensison Biofilm Formation byStreptococcus mutans

2018 ◽  
Author(s):  
Yesol Yoo ◽  
Dong-Ho Seo ◽  
Hyunjin Lee ◽  
Young-Do Nam ◽  
Myung-Ji Seo
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Culture Medium ◽  
Focal Point ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Inhibitory Effect ◽  
Fermented Foods ◽  
Bacillus Velezensis

ABSTRACTStreptococcus mutansplays a key role in the development of dental caries and promotes the formation of oral biofilm produced by glucosyltransferases (GTFs).Bacillus velezensisK68 was isolated from traditional fermented foods and inhibits biofilm formation mediated byS. mutans. Gene amplification results demonstrated thatB. velezensisK68 contained genes for the biosynthesis of 1-deoxynojirimycin (1-DNJ), a known GTF expression inhibitor. The presence of the GabT1, Yktc1, and GutB1 genes required for 1-DNJ synthesis inB. velezensisK68 was confirmed. Supernatant fromB. velezensisK68 culture medium inhibited biofilm formation by 84% whenS. mutanswas cultured for 48 h, and inhibited it maximally when 1% glucose was added to theS. mutansculture medium as a GTF substrate. In addition, supernatant fromB. velezensisK68 medium containing 3 ppb 1- DNJ decreasedS. mutanscell surface hydrophobicity by 79.0 ± 0.8% compared with that of untreated control. The supernatant containing 1-DNJ decreasedS. mutansadherence by 99.97% and 98.83% under sugar-dependent and sugar-independent conditions, respectively.S. mutanstreated with the supernatant exhibited significantly reduced expression of the essential GTF genesgtfB,gtfC,andgtfDcompared to that in the untreated group. Thus,B. velezensisinhibits the biofilm formation, adhesion, and GTF gene expression ofS. mutansthrough 1- DNJ production.IMPORTANCEDental caries is among the most common infectious diseases worldwide, and its development is closely associated with physiological factors of bacteria, such as the biofilm formation and glucosyltransferase production ofStreptococcus mutans.Biofilms are difficult to remove once they have formed due to the exopolysaccharide matrix produced by the microorganisms residing in them; thus, inhibiting biofilm formation is a current focal point of research into prevention of dental caries. This study describes the inhibitory properties ofBacillus velezensisK68, an organism isolated from traditional Korean fermented foods, against biofilm formation byS. mutans. Herein, we show thatB. velezensisinhibits the biofilm formation, adherence to surfaces, and glucosyltransferase production ofS. mutans.

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