scholarly journals Antimicrobial Activity and HPLC Fingerprinting of CrudeOcimumExtracts

2011 ◽  
Vol 8 (3) ◽  
pp. 1430-1437 ◽  
Author(s):  
S. S. Deo ◽  
F. Inam ◽  
R. P. Mahashabde
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Ocimum Sanctum ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Gram Positive ◽  
Strong Activity ◽  
Gram Negative ◽  
E Coli ◽  
Strong Antimicrobial Activity

The antimicrobial activity of crude methanolic and aqueous extracts ofOcimum sanctumandOcimum kilimandsacharicumagainst gram positive, gram negative and antifungal activity was evaluated to find the zone of inhibition and to set a HPLC profile or fingerprint of these extracts. The crude methanolic extract ofOcimum sanctumshowed strong antimicrobial activity againstS.aureusandC. albicansand moderate activity againstE. coliandB. subtilis. The crude methanolic extract ofOcimum kilimandsacharicumshowed strong antimicrobial activity againstS. aureus, E. coliandC. albicansat higher concentration, same as that shown by the standard forC. albicans. It showed moderate activity againstB. subtilis. The crude aqueous extracts of Ocimum sanctum showed strong antimicrobial activity againstS.aureusand moderate against others. Whereas the crude aqueous extracts ofOcimum kilimandsacharicumshowed moderate activity against the gram positive and gram negative organisms and strong activity againstC. albicansat higher concentration, same as that shown by the standard forC. albicans.

scholarly journals Syntheses, Characterization and Antimicrobial Evaluation of Some 1, 3, 5-Trisubustituted Pyrazole Derivatives

2010 ◽  
Vol 7 (4) ◽  
pp. 1190-1195 ◽  
Author(s):  
Vertika Gautam ◽  
Viney Chawla ◽  
Pankaj k. Sonar ◽  
Shailendra K. Saraf
Keyword(s):  
Antimicrobial Activity ◽  
Moderate Activity ◽  
Gram Negative Bacteria ◽  
Pyrazole Derivatives ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antimicrobial Evaluation

A series of 1, 3, 5-trisubustituted pyrazole derivatives were synthesized and screened for antimicrobial activity. The compounds(2j-o)were evaluated against two gram-positive and two gram-negative bacteria and one fungus, at concentrations of 10 µg/mL and 50 µg/mL. The compounds were founds to be inactive againstP. aeruginosaandA. nigerbut exhibited moderate activity againstB. subtilis, E. coliandS. aureus. It can be concluded that the newly synthesized compounds possess promising antimicrobial activity.

scholarly journals Synthesis and antimicrobial activity of some new pyrazoline derivatives bearing sulfanilamido moiety

2019 ◽  
Vol 10 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Maysoon Mohammed Almahdi ◽  
Ahmed Elsadig Mohammed Saeed ◽  
Nadia Hanafy Metwally
Keyword(s):  
Antimicrobial Activity ◽  
Moderate Activity ◽  
Gram Positive ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli ◽  
Chemical Structures ◽  
In Vitro Antibacterial Activity ◽  
Ft Ir

In the present study, a series of new pyrazoline derivatives bearing sulfanilamido moiety were synthesized and obtained in good yields. The chemical structures of the compounds were elucidated by spectral data (FT-IR, MS, UV-VIS and NMR). The synthesized compounds 41-70 were screened for their antimicrobial activity and compared with controls. The in vitro antibacterial activity of compounds 41-45 and 48-57 was checked against two Gram positive microorganisms (S. aureus and S. mutans) and three Gram negative microorganisms (E. coli, K. pneumonia and P. aureginosa), their antifungal activity was checked against C. albicans. The preliminary results showed that these compounds had moderate activity against the tested organisms. Compounds 41, 48, 51 and 56 exhibited promising antimicrobial activity against S. aureus compared to standard drug Ampicilin. Final synthesized compounds 58-70 were tested against two Gram positive (S. aureus and B. subtilis) and two Gram negative (E. coli and P. aureginosa) microorganisms, their activity against C. albicans was also checked and they did not exhibit any antimicrobial activity.

scholarly journals Screening Antimicrobial Activity of Nickel Nanoparticles Synthesized Using Ocimum sanctum Leaf Extract

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Chitra Jeyaraj Pandian ◽  
Rameshthangam Palanivel ◽  
Solairaj Dhanasekaran
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Nickel Nanoparticles ◽  
Ocimum Sanctum ◽  
Ros Generation ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Bacteria And Fungi

Antimicrobial efficacy of nickel nanoparticles synthesized using leaf extract of Ocimum sanctum (NiGs) was investigated against pathogenic Gram-negative (E. coli, K. pneumoniae, and S. typhi), Gram-positive (B. subtilis, S. epidermidis) bacteria and fungi (C. albicans, C. tropicalis, A. fumigatus, A. clavatus, and A. niger). 100 µg/mL NiGs showed maximum antimicrobial activity against tested pathogens compared to leaf extract and antibiotics. E. coli (25 mm) and C. albicans (23 mm) exhibited higher zone of inhibition at 100 µg/mL NiGs. MIC, MBC, and MFC values of NiGs against all tested pathogens ranged between 25 and 50 µg/mL. Growth of bacterial and fungal cells (105 cfu/mL) was completely inhibited at 50 µg/mL NiGs. E. coli and C. albicans have showed strong antimicrobial activity with 81% and 50% reactive oxygen species (ROS) production, 30 and 16 µg/mL protein leakage, and 95 and 82 U/L LDH leakages, respectively. Gram-negative bacteria and Candida species showed more sensitivity to NiGs at all concentrations tested (25–100 µg/mL) than Gram-positive bacteria and Aspergillus species, respectively. Microbial growth in the presence of NiGs and ascorbic acid confirmed the involvement of ROS in antimicrobial activity. Hence, NiGs induced ROS generation was attributed to the protein and LDH leakage from microbial membranes.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals Synthesis of Plant-Mediated Silver Nanoparticles UsingDioscorea batatasRhizome Extract and Evaluation of Their Antimicrobial Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
P. C. Nagajyothi ◽  
K. D. Lee
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Room Temperature ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Synthesize Silver Nanoparticles ◽  
Dioscorea Batatas

The eco-friendly synthesis of nanoparticles through various biological means helps to explore various plants for their ability to synthesize silver nanoparticles (AgNPs). Here we have synthesized AgNPs by using rhizome extract ofDioscorea batatasat as well as room temperature (). AgNPs were characterized under UV-vis spectrophotometer, SEM, FTIR, XRD, and EDX. The antimicrobial activity of AgNPs was evaluated on gram positive (B. substilisandS. aureus), gram negative (E. coli), and fungi (S. cerivisaeandC. albicans). At room temperature,S. cerivisaeandC. albicanswere found to be more susceptible to AgNPs than at .

scholarly journals Antimicrobial Potential of Camellia sinensis against Skin Associated Microbial Pathogens

2019 ◽  
pp. 413-419
Author(s):  
Lovey Sharma ◽  
Ram Kumar Pundir
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Petroleum Ether ◽  
Camellia Sinensis ◽  
Methanolic Extract ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Propionibacterium Acne ◽  
Antimicrobial Potential

The present study was undertaken to assess the antimicrobial potential of leaves’ extract of Green tea (Camellia sinensis) against skin associated microorganisms. The antimicrobial activity was studied through agar well diffusion method against Gram positive, Gram negative bacteria and fungus included Propionibacterium acne, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans in five different solvents viz. Methanol, Ethanol, Distilled water, Chloroform and Petroleum ether. The methanolic extract of Camellia sinensis showed maximum zone of inhibition against Propionibacterium acne (27 mm) and S. aureus (26 mm); ethanolic extract was most effective against Candida albicans (22 mm) and petroleum ether extract was most effective against S. aureus (20 mm). The antimicrobial analyses showed that the extracts were more effective against Gram positive bacteria than Gram negative bacteria and fungus. Comparative study of antimicrobial activity of Camellia sinensis extracts with antibiotics revealed that Camellia sinensis methanolic extract had maximum effective antimicrobial activity against all the tested microorganisms. MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of methanolic extract of Camellia sinensis against the microbial agents under study ranged between 0.125 to 0.015625 mg/ml. The synergistic interaction of Camellia sinensis with antibiotics revealed much better results as compared to antibiotics susceptibility pattern alone. Phytochemicals analyses of Camellia sinensis included alkaloids, saponins, glycosides, proteins, phenols, tannins and phytosterols. The compounds identified by GC-MS analysis of methanolic extract of Camellia sinensis were reported to be used as water treatment, antifungals, antimicrobials and enzymatic inhibitors, improve mental alertness, antifungal, anti-HIV, anticancer, anti-inflammatory, analgesic and comonomer (e.g. for lubricating oil additives). Therefore, it may be concluded that Camellia sinensis leaves possess antibacterial and antifungal properties, which may be used as alternate drug of choice due to its lower side-effects to human skin as compared to presently used therapeutic agents.

scholarly journals Hybrid Approach for Synthesis and Antimicrobial Activity of Heterocyclic Compounds

2018 ◽  
Vol 77 ◽  
pp. 35-52 ◽  
Author(s):  
Nisheeth C. Desai ◽  
Darshita V. Vaja
Keyword(s):  
Antimicrobial Activity ◽  
Heterocyclic Compounds ◽  
Hybrid Approach ◽  
Spectroscopic Techniques ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Microbial Strains

We have synthesized novel series of N-(1-(2-(1-phenyl-3-(p-tolyl)-1H-pyrazol-4-yl)-5-(pyridin-4-yl)-1,3,4-oxadiazol-3(2H)-yl)ethylidene)arylaniline and their derivatives. The structures of synthesized compounds were well characterized by spectroscopic techniques. Antimicrobial activity of the newly synthesized derivatives was evaluated against gram positive (S. aureus and S. pyogenes), gram negative bacteria (E. coli and P. aeruginosa), and strains of fungi (C. albicans, A. niger and A. clavatus). Among the screened derivatives 5c, 5f, 5i, 5l and 5t demonstrated superior antimicrobial activity against microbial strains.

Antimicrobial activities of Antarctic sponges

1992 ◽  
Vol 4 (2) ◽  
pp. 179-183 ◽  
Author(s):  
J.B. McClintock ◽  
J.J. Gauthier
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Antimicrobial Activities ◽  
Marine Sponges ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Strong Activity ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Antarctic Marine

Methanol-toluene extracts of 17 common Antarctic marine sponges collected from shallow waters in McMurdo Sound in October–December 1989 were tested for suppression of growth of bacteria (gram-positive and negative), yeasts and fungi. Weak to moderate levels of antimicrobial activity occurred in all sponges. Antimicrobial activity was more common when gram-negative bacteria were exposed to sponge extracts; 47% of the sponge extracts caused growth inhibition in one or more gram-positive bacteria, while 100% of the extracts caused growth inhibition in gram-negative bacteria. Particularly strong activity was observed against two species of gram-positive bacteria exposed to extracts of the sponge Latrunculia apicalis and against one strain of gram-negative bacterium exposed to extracts of the sponge Haliclona sp. Antimicrobial responses against yeasts and fungi were generally non-existent or weak, with the exception of the yeast Candida tropicalis, which was strongly inhibited by extracts of the sponges Homaxonella balfourensis, Dendrilla membranosa, Kirkpatrickia variolosa, Gellius benedeni, Cinachyra antarctica and Scolymastia joubinia. Antimicrobial activity in these polar sponges is widespread but generally weaker than that found in temperate and tropical sponges.

Synthesis and Evaluation of Chiral Rhodanine Derivatives Bearing Quinoxalinyl Imidazole Moiety as ALK5 Inhibitors

2021 ◽  
Vol 17 ◽  
Author(s):  
Li-Min Zhao ◽  
Fang Yan Guo ◽  
Hui Min Wang ◽  
Tong Dou ◽  
Jun Da Qi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Positive Control ◽  
Antifungal Drugs ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Design And Synthesis ◽  
Pathway Inhibition ◽  
P38α Map Kinase ◽  
Strong Antimicrobial Activity

Background: TGF-β signaling pathway inhibition is considered an effective way to prevent the development of several diseases. In the design and synthesis of TGF-β inhibitors, a rhodanine compound containing a quinoxalinyl imidazole moiety was found to have strong antimicrobial activity. Objective: The purpose of this work was to investigate the antimicrobial activity of other chiral rhodanine TGF-β inhibitors synthesized. Methods: Two series of 3-substituted-5-((5-(6-methylpyridin-2-yl)-4-(quinoxalinyl-6-yl)- 1H-imidazol-2-yl)methylene)-2-thioxothiazolin-4-ones (12a–h and 13a–e) were synthesized and evaluated for their ALK5 inhibitory and antimicrobial activity. The structures were confirmed by their 1H NMR, 13C NMR, and HRMS spectra. All the synthesized compounds were screened against Gram-positive strains, Gram-negative strains, and fungi. Results: Among the synthesized compounds, compound 12h showed the highest activity (IC50 = 0.416 μM) against ALK5 kinase. Compound 12h exhibited a good selectivity index of > 24 against p38α MAP kinase and was 6.0-fold more selective than the clinical candidate, compound 2 (LY-2157299). Nearly all the compounds displayed high selectivity toward both Gram-positive and Gram-negative bacteria. They also showed similar or 2.0-fold greater antifungal activity (minimum inhibitory concentration [MIC] = 0.5 µg/mL) compared with the positive control compounds Gatifloxacin (MIC = 0.5 µg/mL) and fluconazole (MIC = 1 µg/mL). Conclusion: The findings suggest that the synthesized rhodanine compounds have good ALK5 inhibitory activity and can be used for further research and development as potential antifungal drugs.

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