scholarly journals Effect of different doses of creatine on the bone in thirty days of supplementation in mice: FT-Raman study

2011 ◽  
Vol 25 (5) ◽  
pp. 225-233 ◽  
Author(s):  
Humberto Miranda ◽  
Renato Aparecido De Souza ◽  
Maira Gaspar Tosato ◽  
Roberto Simão ◽  
Murilo Xavier Oliveira ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Tissue ◽  
Creatine Supplementation ◽  
High Sensitivity ◽  
Bone Diseases ◽  
Carbonate Content ◽  
Carbonate Apatite ◽  
Tissue Composition ◽  
Different Doses ◽  
Ft Raman

In this study, Raman spectroscopy was employed in order to provide information about the effects of different doses of creatine on bone tissue composition of phosphate apatite (960 cm–1), carbonate apatite (1170 cm–1) representing the mineral content and collagen matrix (amide I, 1665 cm–1). The animals (27 Balb-C male) were divided into three groups (n═ per group): control (CON), supplemented with 0.5 g/kg (Cre-0.5) and with 2.0 g/kg (Cre-2.0) creatine. The experiment was carried out for thirty days. After this time, the right femur of each animal was harvested. The specimens were assessment by FT-Raman spectroscopy and in a total of 81 spectra were acquired in the medial diaphysis of the femur. The Raman data strongly suggest that only the creatine supplementation of 0.5 g/kg effective to the bone constitution. Furthermore, the present results demonstrate that creatine ingestion provokes decrease in the relative presence of carbonate in the chemical constitution of bones. The decrease in the carbonate content can be associated to a significantly bone resistance altered to several mammalians. The analysis evidenced that the mineral concentrations in the Raman spectroscopy could be a feasible method for non-invasive or minimally invasive assessment of bone tissue composition. Probably this high sensitivity can be employed to determine spectral profiles, such as wavelength of maximum absorption and maximum intensity of absorption of each wavelength, of several bone diseases.

Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study

2011 ◽  
Vol 27 (2) ◽  
pp. 487-495 ◽  
Author(s):  
Renato Aparecido de Souza ◽  
Murilo Xavier ◽  
Fabiano Fernandes da Silva ◽  
Marco Túlio de Souza ◽  
Maira Gaspar Tosato ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Quality ◽  
Rat Model ◽  
Creatine Supplementation ◽  
Ovariectomized Rat ◽  
Spectroscopy Study ◽  
Ft Raman Spectroscopy ◽  
Ft Raman

scholarly journals Effects of Different Swimming Exercise Intensities on Bone Tissue Composition in Mice: A Raman Spectroscopy Study

2011 ◽  
Vol 29 (4) ◽  
pp. 217-225 ◽  
Author(s):  
Fabiano Fernandes da Silva ◽  
Renato Aparecido de Souza ◽  
Marcos Tadeu Tavares Pacheco ◽  
Wellington Ribeiro ◽  
Marcos Augusto Souza Rodrigues da Silva ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Tissue ◽  
Swimming Exercise ◽  
Spectroscopy Study ◽  
Tissue Composition

scholarly journals In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4275
Author(s):  
Tetsuya Adachi ◽  
Francesco Boschetto ◽  
Nao Miyamoto ◽  
Toshiro Yamamoto ◽  
Elia Marin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Tissue ◽  
Bone Defects ◽  
Bone Diseases ◽  
Therapeutic Modality ◽  
3D Scaffold ◽  
Computed Tomography Scanning ◽  
Large Bone ◽  
Large Bone Defects

The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.

scholarly journals The potential of carbonate apatite as an alternative bone substitute material

2019 ◽  
Vol 28 (1) ◽  
pp. 92-7
Author(s):  
Ahmad Jabir Rahyussalim ◽  
Sugeng Supriadi ◽  
Aldo Fransiskus Marsetio ◽  
Pancar Muhammad Pribadi ◽  
Bambang Suharno
Keyword(s):  
Bone Graft ◽  
Bone Tissue ◽  
Bone Substitute ◽  
Chemical Reactivity ◽  
Donor Site ◽  
Microstructural Analysis ◽  
Carbonate Content ◽  
Carbonate Apatite ◽  
Tissue Formation ◽  
Common Procedure

Bone reconstructive surgery has become a common procedure, and bone transplantation has become the second most frequently performed tissue transplantation procedure worldwide. Therefore, the need for bone substitute materials has increased. Artificial bone substitutes exhibit osteoconductive properties and feature several advantages, including abundant resources, low cost, and low donor site morbidity. Carbonate apatite (CO3Ap) is a calcium phosphate ceramic that can be used as a synthetic bone graft. The carbonate content of this ceramic is similar to that of bone apatite. In this review, we show that carbonate apatite can be degraded given its chemical reactivity in a weakly acidic environment and through osteoclast resorption. Moreover, it is osteoconductive and promotes bone tissue formation without fibrotic tissue formation. Additionally, microstructural analysis revealed that new bone tissue is formed within the bone graft itself.

Lactoferrin in Bone Tissue Regeneration

2020 ◽  
Vol 27 (6) ◽  
pp. 838-853 ◽  
Author(s):  
Madalina Icriverzi ◽  
Valentina Dinca ◽  
Magdalena Moisei ◽  
Robert W. Evans ◽  
Mihaela Trif ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Cells ◽  
Bone Diseases ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Bone Homeostasis ◽  
Active Compounds

: Among the multiple properties exhibited by lactoferrin (Lf), its involvement in bone regeneration processes is of great interest at the present time. A series of in vitro and in vivo studies have revealed the ability of Lf to promote survival, proliferation and differentiation of osteoblast cells and to inhibit bone resorption mediated by osteoclasts. Although the mechanism underlying the action of Lf in bone cells is still not fully elucidated, it has been shown that its mode of action leading to the survival of osteoblasts is complemented by its mitogenic effect. Activation of several signalling pathways and gene expression, in an LRPdependent or independent manner, has been identified. Unlike the effects on osteoblasts, the action on osteoclasts is different, with Lf leading to a total arrest of osteoclastogenesis. : Due to the positive effect of Lf on osteoblasts, the potential use of Lf alone or in combination with different biologically active compounds in bone tissue regeneration and the treatment of bone diseases is of great interest. Since the bioavailability of Lf in vivo is poor, a nanotechnology- based strategy to improve the biological properties of Lf was developed. The investigated formulations include incorporation of Lf into collagen membranes, gelatin hydrogel, liposomes, loading onto nanofibers, porous microspheres, or coating onto silica/titan based implants. Lf has also been coupled with other biologically active compounds such as biomimetic hydroxyapatite, in order to improve the efficacy of biomaterials used in the regulation of bone homeostasis. : This review aims to provide an up-to-date review of research on the involvement of Lf in bone growth and healing and on its use as a potential therapeutic factor in bone tissue regeneration.

scholarly journals Overview of Popular Techniques of Raman Spectroscopy and Their Potential in the Study of Plant Tissues

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1537
Author(s):  
Aneta Saletnik ◽  
Bogdan Saletnik ◽  
Czesław Puchalski
Keyword(s):  
Raman Spectroscopy ◽  
Cell Walls ◽  
Structural Changes ◽  
Spatial Information ◽  
Technological Development ◽  
Analytical Techniques ◽  
High Sensitivity ◽  
Plant Tissues ◽  
Wide Range ◽  
Identification Technique

Raman spectroscopy is one of the main analytical techniques used in optical metrology. It is a vibration, marker-free technique that provides insight into the structure and composition of tissues and cells at the molecular level. Raman spectroscopy is an outstanding material identification technique. It provides spatial information of vibrations from complex biological samples which renders it a very accurate tool for the analysis of highly complex plant tissues. Raman spectra can be used as a fingerprint tool for a very wide range of compounds. Raman spectroscopy enables all the polymers that build the cell walls of plants to be tracked simultaneously; it facilitates the analysis of both the molecular composition and the molecular structure of cell walls. Due to its high sensitivity to even minute structural changes, this method is used for comparative tests. The introduction of new and improved Raman techniques by scientists as well as the constant technological development of the apparatus has resulted in an increased importance of Raman spectroscopy in the discovery and defining of tissues and the processes taking place in them.

On-Line Monitoring of Airborne Chemistry in Levitated Nanodroplets:  In Situ Synthesis and Application of SERS-Active Ag−Sols for Trace Analysis by FT-Raman Spectroscopy

2003 ◽  
Vol 75 (9) ◽  
pp. 2166-2171 ◽  
Author(s):  
Nicolae Leopold ◽  
Michael Haberkorn ◽  
Thomas Laurell ◽  
Johan Nilsson ◽  
Josefa R. Baena ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Trace Analysis ◽  
In Situ Synthesis ◽  
Ft Raman Spectroscopy ◽  
On Line ◽  
Ft Raman

Application of FT-Raman spectroscopy to the study of selected organometallic complexes and proteins

1990 ◽  
Vol 68 (7) ◽  
pp. 1196-1200 ◽  
Author(s):  
Steven M. Barnett ◽  
François Dicaire ◽  
Ashraf A. Ismail
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Metal Carbonyl ◽  
Protein A ◽  
Organometallic Complexes ◽  
Laser Source ◽  
Chromium Tricarbonyl ◽  
Ft Raman Spectroscopy ◽  
Arene Chromium Tricarbonyl ◽  
Ft Raman

The study of colored organometallic complexes by dispersive Raman spectroscopy has been limited due to fluorescence or photodecomposition caused by the visible laser used as the excitation source. As a solution to this problem, FT-Raman spectroscopy with a near-infrared laser source has been useful in lowering fluorescence or photolysis in these samples. To investigate the utility of this technique, we have obtained and assigned the FT-Raman spectra of a series of arene chromium tricarbonyl complexes and of cyclopentadienyl manganese tricarbonyl. Some bands previously unobserved by dispersive Raman spectroscopy were seen, including a band assigned to a 13CO satellite in the spectrum of methylbenzoate chromium tricarbonyl. In addition, FT-Raman data for bovine serum albumin (BSA) and Protein-A are presented. Keywords: FT-Raman spectroscopy, metal carbonyl, proteins, organometallics, near infrared.

FT-Raman spectroscopy of titania hydrolysates

1997 ◽  
Vol 53 (7) ◽  
pp. 969-977 ◽  
Author(s):  
Paul A. Venz ◽  
Ray L. Frost ◽  
J.R. Bartlett ◽  
J.L. Woolfrey
Keyword(s):  
Raman Spectroscopy ◽  
Ft Raman Spectroscopy ◽  
Ft Raman
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