scholarly journals Molecular Typing ofNeisseria gonorrhoeaeIsolates by Opa-Typing and Ribotyping in New Delhi, India

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Pejvak Khaki ◽  
Preena Bhalla ◽  
Ahmad Mir Fayaz ◽  
Sohiela Moradi Bidhendi ◽  
Majid Esmailzadeh ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Preventive Measures ◽  
Restriction Enzymes ◽  
New Delhi ◽  
Pcr Product ◽  
Sexual Contacts ◽  
High Level ◽  
Epidemiological Characteristics ◽  
Epidemiological Characterization

Control and preventive measures for gonococcal infections are based on precise epidemiological characteristics ofN. gonorrhoeaeisolates. In the present study the potential utility of opa-typing and ribotyping for molecular epidemiological study of consecutive gonococcal strains was determined. Sixty gonococcal isolates were subjected to ribotyping with two restriction enzymes,AvaII andHincII, and opa-typing withTaqI andHpaII for epidemiological characterization of gonococcal population. Ribotyping withAvaII yielded 6 ribotype patterns while twelve RFLP patterns were observed withHincII. Opa-typing of the 60 isolates revealed a total 54 opa-types, which 48 were unique and 6 formed clusters. Fifty-two opa-types were observed withTaqI-digested PCR product while opa-typing withHpaII demonstrated 54 opa-types. The opa-types from isolates that were epidemiologically unrelated were distinct, whereas those from the sexual contacts were identical. The results showed that opa-typing is highly useful for characterizing gonococcal strains from sexual contacts and has more discriminatory than ribotyping that could differentiate between gonococci of the same ribotype. The technique even with a single restriction enzyme has a high level of discrimination (99.9%) between epidemiologically unrelated isolates. In conclusion, the molecular methods such as opa-typing and ribotyping can be used for epidemiological characterization of gonococcal strains.

Transplantes de córneas no Rio Grande do Norte: aspectos epidemiológicos e clínicos

2016 ◽  
Vol 15 ◽  
pp. 579
Author(s):  
Giovanna Karinny Pereira Cruz ◽  
Marcos Antonio Ferreira Júnior
Keyword(s):  
Rio Grande ◽  
Waiting List ◽  
Corneal Tissue ◽  
Cross Sectional ◽  
Condition Indicator ◽  
Epidemiological Characteristics ◽  
Tissue Transplant ◽  
Epidemiological Characterization ◽  
Corneal Disorder

Aim: To determine the clinical and epidemiological characteristics as well  as the  distribution  of  patients  who underwent  or  are in  the  waiting list  for  corneal tissue transplantation in a main medical facility for the referred service in the state of Rio Grande do Norte. Methods: An epidemiological, quantitative, cross-sectional, descriptive and  analytical  study  comprising  all  the  patients  on  the  waiting  list  for  transplantation (n=62 patients) and the ones who underwent corneal tissue transplant surgery (n=258) in  a  specialized  health  service  center.  Results:  The  profile  of  the  patients  presented differences in relation to gender and similarities in characteristics such as age, origin and condition indicator for transplantation. T he “type of corneal disorder” variable stood out due  to  its  statistical  association  with  clinical  and  epidemiological  variables.  Conclusion: The  clinical  and  epidemiological  characterization  of  the  population  subject  to  cornea transplantation  allows  the  identification  and  inference  of  possible  risk  factors  for  this procedure with a direct relation to their survival.

scholarly journals Molecular Characterization of Stolbur Group Subgroup E (16SrXII-E) Phytoplasma Associated with Potatoes in China

Plant Disease ◽  
2012 ◽  
Vol 96 (9) ◽  
pp. 1372-1372 ◽  
Author(s):  
M. Cheng ◽  
J. Dong ◽  
L. Zhang ◽  
P. J. Laski ◽  
Z. Zhang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Inner Mongolia ◽  
Disease Incidence ◽  
Restriction Enzymes ◽  
Potato Disease ◽  
Growing Seasons ◽  
Pcr Product ◽  
Disease Surveys

Phytoplasmas have been reported from more than 70 plant species in China, most of which are from woody plants and very few are from potatoes (Solanum tuberosum). During the growing seasons of 2005 through 2011, potato disease surveys were conducted in seed and commercial fields in Yunnan Province and Inner Mongolia. Potato plants displayed symptoms of curled, yellowish and purplish leaves, shortened internodes, aerial tuber formation, and few small malformed underground tubers. Although the location of the fields surveyed each year varied, the disease seems to have become increasingly prevalent. In Yunnan, disease incidence was 5 to 24% in 2005 and 15 to 100% in 2010 and 2011. In Inner Mongolia, disease incidence in seed potato fields was 5 to 15% in 2006 and 25 to 50% in 2011. Total DNA was extracted from the leaves, stems, and roots of symptomatic and asymptomatic plants with a DNeasy Plant Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's instruction. A nested PCR was performed by using primer pair P1/P7 followed by R16F2n/R16R2 to detect the presence of phytoplasmas (1,3). An approximate 1.25-kb PCR product was amplified from symptomatic plants but not from asymptomatic plants. Restriction fragment length polymorphism (RFLP) patterns were analyzed by digesting the 1.2-kb amplicon singly with restriction enzymes AluI, BfaI, HhaI, HpaI, KpnI, MseI, and TaqI. Comparing the RFLP patterns of samples with previously published phytoplasma strains, the phytoplasmas matched patterns of the stolbur group, subgroup E (16SrXII-E) (1). In addition, the PCR product from P1/P7, diluted 1:30, was amplified by using primer pair P1A/P7A (2). The nested PCR product was cloned into pCR8/GW/TOPO vector (Invitrogen, Carlsbad, CA) and sequenced by the Core Lab of the University of Alaska Fairbanks and GENEWIZ (South Plainfield, NJ). Nucleotide sequences (GenBank Accession No. EU293841) were analyzed by iPhyClassifier software (4), confirming the relationship of this phytoplasma to ‘Candidatus Phytoplasma fragariae’ with RFLP patterns identical to group 16SrXII-E. To our knowledge, this is the first molecular characterization of the stolbur group phytoplasmas associated with potato disease in China. The potato is becoming increasingly important in China. The impacts of stolbur on potato yield losses, disease distributions, and insect vectors are currently under investigation. References: (1) I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998. (2) I.-M. Lee et al. Int. J. Syst. Evol. Microbiol 54:337, 2004. (3) C. D. Smart et al. Appl. Environ. Microbiol. 62:2988, 1996. (4) Y. Zhao et al. Int. J. Syst. Evol. Microbiol. 59:2582, 2009.

scholarly journals Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

2011 ◽  
Vol 48 (1) ◽  
pp. 3-7 ◽  
Author(s):  
S. Simsek ◽  
A. Utuk ◽  
I. Balkaya
Keyword(s):  
Fasciola Hepatica ◽  
Restriction Enzymes ◽  
Rapid Pcr ◽  
Domestic Ruminants ◽  
Pcr Product ◽  
Rflp Profile ◽  
The Common ◽  
Serious Disease ◽  
Liver Flukes ◽  
Epidemiological Characteristics

AbstractThe most common and widespread liver flukes of the genus Fasciola are Fasciola hepatica and F. gigantica. Adults of both species occur in many domestic ruminants and in humans and can cause serious disease. The differential diagnosis of these flukes infection is very important because of their different transmission and epidemiological characteristics. A simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the common restriction enzymes AluI and RsaI, is described to distinguish between both fasciolid species. After the digestion of the mitochondrial cytochrome c oxidase 1 (CO1) PCR product with the restriction enzyme AluI, the RFLP profile obtained from F. hepatica revealed two fragments, whereas F. gigantica was not cut. The RsaI digestion generated two fragments from F. gigantica, whereas it did not cut the PCR product from F. hepatica. Results were confirmed with CO1 sequence analysis of both F. hepatica and F. gigantica. The present study suggests that the PCRRFLP method described here can be used for the proper identification of Fasciola species.

scholarly journals Epidemiological and Molecular Characterization of Echinococcus granulosus Isolated from Small Ruminants in Kashmir Valley, India

2021 ◽  
Author(s):  
Akeel Beigh ◽  
Mohmommad Darzi ◽  
Samina Bashir ◽  
Parvaiz Dar ◽  
Nazir Ganai ◽  
...  
Keyword(s):  
Small Ruminants ◽  
Restriction Enzymes ◽  
Economic Problem ◽  
Kashmir Valley ◽  
Predominant Genotype ◽  
Pcr Product ◽  
Relative Prevalence ◽  
Female Sheep ◽  
Sheep Strain

Background: Cystic Echinococcosis (CE) is an emergent or re-emergent zoonosis and remains a public health and economic problem all over the world. Methods:  The present study was carried on the prevalence and genotypes of Echinococcus present in small ruminants in Kashmir valley. A total of 2100, sheep (2052) and goats (48), slaughtered or spontaneously dead, from various areas of Kashmir valley were screened for the presence of hydatidosis. In case of goat none of the cases were found positive for hydatidosis, whereas, all the positive cases (85) were recorded in sheep only. The overall prevalence of hydatidosis was 4.04%. The prevalence was higher in female sheep (5.46%) compared to males (2.83%). Season-wise highest prevalence was in summer (4.55%), followed by autumn (4.1%), spring (3.89%) and winter (2.5%).The liver was observed to be the most frequently infected organ with relative prevalence of 61.17% followed by lungs (38.82%).The rDNA-ITS1 fragment of positive samples was amplified with BD1 / 4S primers. Results: The length of amplified fragment for all isolated samples was 1000bps. The products obtained on PCR were digested with four restriction enzymes (Rsa 1, Alu 1, Msp 1 and Taq1). Rsa 1, Alu 1, Msp 1 yielded identical fragments, 300 and 700 bp in sheep. TaqI restriction enzyme had no effect on PCR product and after digestion; intact 1000bps fragment was seen. Conclusion: Phylogenetic analysis of ITS1 gene revealed that the common sheep strain (G1) is the predominant genotype in sheep in Kashmir valley.

Epidemiological Characterization of the Infection by Treponema Pallidum in Pregnant Women in Beira City, Sofala, Mozambique

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nchowela Guido
Keyword(s):  
Pregnant Women ◽  
Congenital Syphilis ◽  
Health Centre ◽  
Treponema Pallidum ◽  
Timely Manner ◽  
Pregnancy And Childbirth ◽  
Epidemiological Characterization ◽  
Active Syphilis ◽  
Structured Questionnaire

Introduction: Syphilis is one of several diseases that can be transmitted during pregnancy and childbirth, which can lead to complications during pregnancy and in the newborn. This is especially so when the pregnant woman is not diagnosed or treated properly and in a timely manner. Methodology: Data from 262 pregnant women prospectively included, aged 18-41years, attended at the Ponta Gêa Health Centre for antenatal clinics has been analyzed from January to September of 2016. In the prospective study, a rapid treponemal and a non-treponemal test were performed. A structured questionnaire was used to collect socio demographic and clinical variables, which was developed from the literature review. Results: The prevalence of active syphilis was 11.8%; the majority of reactive pregnant women were aged 18-25years (55.4%), (61.3%) of pregnant women were treated with doses below those recommended; only a small number of the partners were treated successfully. The highest seroprevalence of syphilis was found in housewives (77%), in those who attended primary education (71.6%) in those who had a monthly income of 1000- 3000 MZN (70.3%), in those with two or more pregnancies (55.7%) and living with someone has husband and wife (63.5%). The syphilis/HIV co-infection rate was high. Conclusion: According to the results obtained in this study, urgent measures are needed to assess the problems encountered and to improve the screening approach, treatment and monitoring of syphilis during pregnancy in order to prevent the cases of congenital syphilis.

Gender aspects of aggressive behaviour in adolescence

2020 ◽  
pp. 45-49
Author(s):  
Dmitriy Ivashinenko ◽  
Elena Burdelova ◽  
Lyubov Ivashinenko
Keyword(s):  
Young People ◽  
Young Women ◽  
Physical Aggression ◽  
Aggressive Behaviour ◽  
Preventive Measures ◽  
Young Men ◽  
Target Audience ◽  
Preventive Work ◽  
Gender Aspects ◽  
High Level

This article presents the results of a study the purpose of which was research of the factors and patterns of aggression in adolescence. Its results are required to find personas, who need preventive work, and features of the system of preventive measures, depending on the structure of the target audience. In 2016 there were 721 respondents who took part in the study, and 1437 in 2019. The method used in this study is the Buss-Durkee test modified by G. V. Rezapkina (BDHI). Results of the study clearly demonstrate that amongst young people there is a high-level spread of severe irritation, especially among young women. Also, the predominance of such components of aggression as negativity and irritation was noted. According to the results, young women more often get irritated than young men, and on the scale of “negativism”, there is no significant differences. Physical aggression was discovered to be more characteristic for young men.

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