scholarly journals Separation and Purification of Two Flavone Glucuronides fromErigeron multiradiatus(Lindl.) Benth with Macroporous Resins

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Zhi-feng Zhang ◽  
Yuan Liu ◽  
Pei Luo ◽  
Hao Zhang
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Sorption Isotherms ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Bioactive Constituents ◽  
Simple Method ◽  
Scale Separation ◽  
Adsorption And Desorption ◽  
Macroporous Resins

Scutellarein-7-O-β-D-glucuronide (SG) and apigenin-7-O-β-D-glucuronide (AG) are two major bioactive constituents with known pharmacological effects inErigeron multiradiatus. In this study, a simple method for preparative separation of the two flavone glucuronides was established with macroporous resins. The performance and adsorption characteristics of eight macroporous resins including AB-8, HPD100, HPD450, HPD600, D100, D101, D141, and D160 have been evaluated. The results confirmed that D141 resin offered the best adsorption and desorption capacities and the highest desorption ratio for the two glucuronides among the tested resins. Sorption isotherms were constructed for D141 resin under optimal ethanol conditions and fitted well to the Freundlich and Langmuir models (R2>0.95). Dynamic adsorption and desorption tests was performed on column packed with D141 resin. After one-run treatment with D141 resin, the two-constituent content in the final product was increased from 2.14% and 1.34 % in the crude extract ofErigeron multiradiatusto 24.63% and 18.42% in the final products with the recoveries of 82.5% and 85.4%, respectively. The preparative separation of SG and AG can be easily and effectively achieved via adsorption and desorption on D141 resin, and the method developed can be referenced for large-scale separation and purification of flavone glucuronides from herbal raw materials.

scholarly journals Preparative Separation and Enrichment of Syringopicroside fromFolium syringaeLeaves with Macroporous Resins

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Xin Liu ◽  
Jianming Wang ◽  
Changxin Zhou ◽  
Lishe Gan
Keyword(s):  
Large Scale ◽  
Packed Column ◽  
Major Constituent ◽  
Preparative Separation ◽  
Simple Method ◽  
Adsorption And Desorption ◽  
Macroporous Resins ◽  
Large Scale Preparation ◽  
Different Temperatures ◽  
Scale Preparation

Syringopicroside is the major constituent inFolium syringaeleaves with known pharmacological activities. In this study, a simple method for preparative separation of syringopicroside fromF. syringaeleaves with macroporous resins was developed. Adsorption characteristics of syringopicroside on six types of macroporous resins, including ADS-8, ADS-17, D141, NKA-9, HPD450, and HPD600, have been compared, among which D141 resin showed the best adsorption and desorption capacities for syringopicroside. Adsorption isotherms were used to D141 resin at different temperatures and fitted well to Langmuir and Freundlich equations. Dynamic adsorption and desorption tests were performed on D141 resin-packed column to optimize the separation process of syringopicroside. After one run with D141 resin, the content of syringopicroside was increased 24-fold from 2.32% to 55.74% with a recovery yield of 92.16%. The chromatographic process optimized in this work avoids toxic organic solvent and, thus, is a promising basis for large-scale preparation of syringopicroside.

scholarly journals Separation and Enrichment of Three Coumarins from Angelicae Pubescentis Radix by Macroporous Resin with Preparative HPLC and Evaluation of Their Anti-Inflammatory Activity

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2664 ◽  
Author(s):  
Yuqiao Yang ◽  
Ruichao Zhu ◽  
Jin Li ◽  
Xuejing Yang ◽  
Jun He ◽  
...  
Keyword(s):  
High Performance ◽  
Pharmaceutical Products ◽  
Raw 264.7 Cells ◽  
Preparative Hplc ◽  
Scale Separation ◽  
Adsorption Dynamics ◽  
Adsorption And Desorption ◽  
Macroporous Resins ◽  
Pseudo Second Order ◽  
Anti Inflammatory

In order to enrich and separate three coumarins (columbianetin acetate, osthole and columbianadin) from Angelicae Pubescentis Radix (APR), an efficient method was established by combining macroporous resins (MARs) with preparative high-performance liquid chromatography (PHPLC). Five different macroporous resins (D101, AB-8, DA-201, HP-20 and GDX-201) were used to assess the adsorption and desorption characteristics of three coumarins. The result demonstrated that HP-20 resin possessed the best adsorption and desorption capacities for these three coumarins. Moreover, the adsorption dynamics profiles of three coumarins were well fitted to the pseudo second order equation (R2 > 0.99) for the HP-20 resin. The adsorption process was described by the three isotherms models including Langmuir (R2 > 0.98, 0.046 ≤ RL ≤ 0.103), Freundlich (R2 > 0.99, 0.2748 ≤ 1/n ≤ 0.3103) and Dubinin Radushkevich (R2 > 0.97). The contents of columbianetin acetate, osthole and columbianadin in the product were increased 10.69-fold, 19.98-fold and 19.68-fold after enrichment, respectively. Three coumarins were further purified by PHPLC and the purities of them reached above 98%. Additionally, the anti-inflammatory effects of these three coumarins were assessed by Lipopolysaccharide (LPS)-induced RAW 264.7 cells. It was found that the production of NO and MCP-1 was obviously inhibited by three coumarins. Columbianetin acetate, osthole and columbianadin could be used as potentially natural anti-inflammatory ingredients in pharmaceutical products. It was concluded that the new method combining MARs with PHPLC was efficient and economical for enlarging scale separation and enrichment of columbianetin acetate, osthole and columbianadin with anti-inflammatory effect from the APR extract.

scholarly journals OPTIMATION OF ENRICHMENT AND PURIFICATION OF POLYPHENOLS FROM BLUEBERRIES (VACCINIUM SPP.) BY MACROPOROUS RESINS XAD-7HP

2017 ◽  
pp. 581
Author(s):  
Xinyao Jiao ◽  
Bin Li ◽  
Xiuyan Zhang ◽  
Qi Zhang ◽  
Ningxuan Gao ◽  
...  
Keyword(s):  
Large Scale ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Solution Concentration ◽  
Ethanol Solution ◽  
Maximal Effect ◽  
Scale Separation ◽  
Macroporous Resins ◽  
Solvent Extraction Method ◽  
Langmuir Isotherm Model

Introduction: Blueberries are rich sources of phenolic compounds and exhibit strong antioxidant activities and a wide variety of biological activities. Methods: Polyphenols were extracted from blueberry by solvent extraction method using ethanol solution. The purification of polyphenols from blueberries (Vaccinium spp.) by macroporous resin XAD-7HP was optimized by adsorption and desorption tests. The cellular antioxidant activities of the purified polyphenols were investigated. Results: The adsorption data indicated that the process was best explained by the Langmuir isotherm model. The best conditions were determined as: blueberry polyphenols concentration, 2.00 mg/g; pH 2.0; ethanol desorption solution concentration, 70 % (v/v); flow rates for feeding and elution, 1 and 2 bed volume (BV)/h, respectively; and a water wash volume of 3 BV. Under these conditions, the purity of the blueberry polyphenols was improved from 46.3 to 86.48 %. The EC50 (concentration for 50 % of maximal effect) and CAA (cellular antioxidant activity) values of the blueberry polyphenols were 151.58 mg/mL and 6.39 µmol QE /100 g sample, respectively.Conclusion: The results demonstrate that purification of polyphenols by macroporous resins XAD-7HP will provide foundation for the development of large-scale separation of blueberry polyphenols in industry.

Isolation of Zingiberene from Ginger Essential Oil by Two-Step Intermittent Silica Gel Column Chromatography

2012 ◽  
Vol 550-553 ◽  
pp. 1666-1670 ◽  
Author(s):  
Yan Wang ◽  
Ai Ling Du ◽  
Ai Qin Du
Keyword(s):  
Essential Oil ◽  
Silica Gel ◽  
Column Chromatography ◽  
Large Scale ◽  
Natural Antioxidant ◽  
Separation And Purification ◽  
Scale Separation ◽  
Combined Procedure ◽  
Potential Approach ◽  
Preparative Isolation

Zingiberene, one of the five major sesquiterpene hydrocarbon which are rich in ginger essential oil, is a natural antioxidant and serves as antiviral and antifertility agent. An efficient preparative isolation process of zingiberene from ginger essential oil has been developed in the present study. The separation experiments have been carried out on an intermittent silica gel column chromatography. After the first run treatment with the chromatography, the zingiberene content in the final product was increased from 35.11% to 60.41%. Afterwards the concentration was purified by using the second silica gel column chromatography. The recovery for the combined procedure was 70.68%, with a final purity 72.93%.Therefore, the separation can be achieved easily and efficiently via the intermittent silica gel column chromatography, and the method will provided a potential approach for large-scale separation and purification of zingiberene for its wide pharmaceutical use.

Hexagonal NaYF4 Nanotube Arrays Obtained via Solvothermal Process

2012 ◽  
Vol 554-556 ◽  
pp. 667-670
Author(s):  
Li Tian ◽  
Jin Liu ◽  
Fei Yu Chen ◽  
Qi Liang Sun
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Heat Stability ◽  
High Heat ◽  
Nanotube Arrays ◽  
Yttrium Nitrate ◽  
Simple Method ◽  
Yttrium Fluoride ◽  
X Ray ◽  
Practical Applications

Hexagonal sodium yttrium fluoride has been successfully synthesized via a facile solvothermal route, using yttrium nitrate, sodium fluoride and polyethanediol as raw materials to react in propanetriol solvent. The as-prepared product was characterized by powder X-ray diffraction, scanning electron microscopy, thermogravimetric and differential thermal analysis, Fourier transform infrared spectrum and energy dispersive X-ray spectrum. The characterization results revealed that the products are hexagonal and denoted as NaYF4. The as-synthesized hexagonal sodium yttrium fluoride composed of hollow-structured nanotubes self-assembled and arrayed orientedly to take on bamboo raft morphology. Hexagonal NaYF4 nanotube arrays exhibited high heat stability. This study provides a simple method to prepare bamboo raft-shaped NaYF4 in large scale, which broads their practical applications.

Adsorption and Desorption Studies of Anthocyanins from Black Peanut Skins on Macroporous Resins

2015 ◽  
Vol 11 (6) ◽  
pp. 841-849 ◽  
Author(s):  
Zhenlei Zhao ◽  
Min Wu ◽  
Qi Jiang ◽  
Yuanyuan Zhang ◽  
Xiulian Chang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Large Scale ◽  
Scale Production ◽  
Adsorption And Desorption ◽  
Peanut Skin ◽  
Macroporous Resins ◽  
Large Scale Production ◽  
Column Adsorption ◽  
Langmuir Isotherm Model ◽  
Peanut Skins

Abstract Adsorption of black peanut skin anthocyanins, a natural pigment, onto various macroporous resins was optimized to develop a potential approach for large-scale production of black peanut skin anthocyanins. Seven different macroporous resins (AB-8, SP-207, LS-305A, HP-20, LS-305, DM-21 and LS-610B) were evaluated for the adsorption properties of the anthocyanins extracted from the black peanut skins of Arachis hypogaea L. The adsorption experiment proved that compared to AB-8, SP-207 and LS-305A, four resins, i.e., LS-610B, DM-21, LS-305 and HP-20 have relatively higher capacity of adsorbing black peanut skin anthocyanins from the aqueous solution, which are verified by the related adsorption experiments. The adsorption isotherm data was successfully described by Langmuir isotherm model and the pseudo-second-order rate model simulates the kinetic process well. Column adsorption and desorption tests further proved DM-21 is a promising adsorbent for field applications to recover black peanut skin anthocyanins from aqueous solution. The overall yield of pigment product was 14% based on dried black peanut skin, and anthocyanins purity is at about 5.7% in the pigment product.

PROTOTIPE APLIKASI PENYIRAMAN TANAMAN MENGGUNAKAN SENSOR KELEMBABAN TANAH BERBASIS MICRO CONTOLLER ATMEGA 328

2019 ◽  
Vol 5 (1) ◽  
pp. 97-106
Author(s):  
Rudi Budi Agung ◽  
Muhammad Nur ◽  
Didi Sukayadi
Keyword(s):  
Soil Moisture ◽  
Growth And Development ◽  
Large Scale ◽  
Simple Method ◽  
Moisture Sensor ◽  
Sensor Development ◽  
Soil Moisture Sensor ◽  
Application Systems ◽  
Harvesting Systems ◽  
Working Principle

The Indonesian country which is famous for its tropical climate has now experienced a shift in two seasons (dry season and rainy season). This has an impact on cropping and harvesting systems among farmers. In large scale this is very influential considering that farmers in Indonesia are stilldependent on rainfall which results in soil moisture. Some types of plants that are very dependent on soil moisture will greatly require rainfall or water for growth and development. Through this research, researchers tried to make a prototype application for watering plants using ATMEGA328 microcontroller based soil moisture sensor. Development of application systems using the prototype method as a simple method which is the first step and can be developed again for large scale. The working principle of this prototype is simply that when soil moisture reaches a certainthreshold (above 56%) then the system will work by activating the watering system, if it is below 56% the system does not work or in other words soil moisture is considered sufficient for certain plant needs.

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