scholarly journals Intramural Ganglion Structures in Esophageal Atresia: A Morphologic and Immunohistochemical Study

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Biagio Zuccarello ◽  
Antonella Spada ◽  
Nunzio Turiaco ◽  
Daniela Villari ◽  
Saveria Parisi ◽  
...  
Keyword(s):  
Esophageal Atresia ◽  
Nervous Tissue ◽  
Chromogranin A ◽  
Tracheoesophageal Fistula ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Esophageal Wall ◽  
Motor Disorders ◽  
Esophageal Dysmotility ◽  
S 100

Introduction and Aim. Disorders of esophageal motility causing dysphagia and gastroesophageal reflux are frequent in survivors to esophageal atresia (EA) and distal tracheoesophageal fistula (TEF). The aim of the present study was to investigate the histologic and immunohistochemical features in both esophageal atretic segments to further understand the nature of the motor disorders observed in these patients.Material and Methods. Esophageal specimens from 12 newborns with EA/TEF and 5 newborns dead of unrelated causes were examined. The specimens were fixed in 5% buffered formalin, included in paraffin and cut in 5 micron sections that were stained with hematoxilin and eosin (H and E), and immunohistochemical stainings for Actin, S-100 protein, Neurofilament, Neuron-Specific-Enolase, Chromogranin A and Peripherin were evaluated under the microscope.Results. In controls, the distribution of the neural elements was rather homogenous at both levels of the esophagus. In contrast, the atretic segments showed quantitative and qualitative differences between them with sparser nervous tissue in the distal one in comparison with the proximal one and with controls.Conclusions. These results further support the assumption that histomorphological alterations of the muscular and nervous elements within the esophageal wall might contribute to esophageal dysmotility in patients surviving neonatal operations for EA/TEF.

scholarly journals An Immunohistochemical Study of Three Equine Pulmonary Granular Cell Tumors

1995 ◽  
Vol 32 (6) ◽  
pp. 730-734 ◽  
Author(s):  
P. R. Bouchard ◽  
C. H. Fortna ◽  
P. H. Rowland ◽  
R. M. Lewis
Keyword(s):  
Neural Crest ◽  
Granular Cell Tumor ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
Cell Tumor ◽  
Human Beings ◽  
S 100 ◽  
Ultrastructural Characteristics ◽  
Leu 7

Granular cell tumor (GCT) is a morphologic designation for tumors of varied histogenesis. Most GCTs in human beings are derived from Schwann cells, and rat meningeal GCTs are believed to originate in the neural crest. Three equine pulmonary GCTs from aged horses were studied immunohistochemically with primary antibodies directed against vimentin, cytokeratins (AE1/AE3), S-100, Leu 7, desmin, and neuron-specific enolase (NSE) using a steptavidin–biotin procedure. All three tumors stained similarly with strong and diffuse staining of neoplastic cells for vimentin and S-100 and negative staining with all other antibodies. On the basis of the immunohistochemical results and the previously described histologic and ultrastructural characteristics, equine pulmonary GCT is designated as neural crest and possibly Schwann cell derived, similar to GCT in rats and human beings.

Laryngotracheoesophageal Cleft: Report of a Case Successfully Treated and Review of the Literature

PEDIATRICS ◽  
1974 ◽  
Vol 53 (4) ◽  
pp. 516-522
Author(s):  
Nathan Burroughs ◽  
Lucian L. Leape
Keyword(s):  
Differential Diagnosis ◽  
Respiratory Distress ◽  
Esophageal Atresia ◽  
Tracheoesophageal Fistula ◽  
Surgical Repair ◽  
Esophageal Wall ◽  
Review Of The Literature ◽  
Persistent Symptoms ◽  
Neonatal Respiratory Distress ◽  
Successful Repair

Laryngotracheoesophageal cleft is a rare but potentially curable anomaly. It should be considered in the differential diagnosis of neonatal respiratory distress aggravated by feeding. Diagnosis is difficult, and repeat endoscopic procedures may be necessary. Immediate surgical repair is indicated. Incorporation of part of the esophageal wall into the repair may enhance success. Tracheostomy has been required in all survivors. Because a cleft may be associated with esophageal atresia and/or tracheoesophageal fistula, its presence should be suspected in patients with these conditions who have persistent symptoms after successful repair.

scholarly journals Cutaneous Plasmacytomas in Dogs: A Morphologic and Immunohistochemical Study

1989 ◽  
Vol 26 (3) ◽  
pp. 216-221 ◽  
Author(s):  
K. E. Baer ◽  
A. K. Patnaik ◽  
S. R. Gilbertson ◽  
A. I. Hurvitz
Keyword(s):  
Cellular Differentiation ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Benign Neoplasms ◽  
Round Cell ◽  
Immunohistochemical Markers ◽  
S 100 ◽  
Round Cell Tumors

Forty-nine cutaneous plasmacytomas in 46 dogs were studied. Tumors occurred at solitary sites in middle-aged to old dogs (mean age, 9.7 years) and most commonly involved the skin of the digits, lips, and ears. Initial diagnosis was made on the basis of light microscopic morphologic findings. Tumors were graded according to the extent of cellular differentiation and immunoreactivity to a panel of immunohistochemical markers (cytokeratins, canine IgG F[ab]2, neurofilament, neuron-specific enolase, S-100 protein, and vimentin). Immunoreactivity was limited to antibodies directed at canine IgG F(ab)2, and vimentin. Vimentin immunoreactivity was usually greater than that of canine IgG F(ab)2, but there was no correlation between immunoreactivity and histologic grade of the tumors. Thirty-six of 39 dogs (92.3%) followed (mean follow-up, 13 months) were cured by surgical excision. The results of this study indicate that canine cutaneous plasmacytomas are benign neoplasms that should be included in the differential diagnosis of cutaneous round cell tumors in dogs.

Granular Cell Tumor: An Immunohistochemical Study

1994 ◽  
Vol 80 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Antonio Cavaliere ◽  
Angelo Sidoni ◽  
Ivana Ferri ◽  
Brunangelo Falini
Keyword(s):  
Granular Cell Tumor ◽  
Immunohistochemical Study ◽  
Recent Observation ◽  
Neuron Specific Enolase ◽  
Benign Neoplasm ◽  
Granular Cell ◽  
Cell Tumor ◽  
Ultrastructural Studies ◽  
S 100 ◽  
Alpha 1 Antitrypsin

Aims and background Granular cell tumor, usually a benign neoplasm, has been the object of many studies because of its uncertain histogenesis and based on many immunohistochemical and ultrastructural studies it has been suggested that it originates from the Schwann cell. Our recent observation that granular cell tumor is positive with PG-M1, a new anti-macrophage monoclonal antibody, led us to further investigate the immunophenotypic profile of the tumor. Study design We studied 11 granular cell tumors using a panel of 20 antibodies, 13 monoclonal and 7 polyclonal. Results The immunohistochemical study showed in all cases a constant diffuse positivity for S-100 protein, neuron-specific enolase, vimentin, KP1 and PG-M1, as well as occasional and focal positivity for alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme. Conclusions The immunophenotypic profile constantly observed could be the expression, on one hand, of the neuroectodermic nature of the neoplasm, proven by positivity for S-100 protein, neuron specific enolase and vimentin, and on the other could be the expression of the phagocytic activity of the tumor cell, proven by positivity for KP1 and PG-M1 antibodies and also by the presence of numerous phagolysosomes.

DOZ047.57: Eosinophilic esophagitis in an infant with repaired esophageal atresia and tracheoesophageal fistula

2019 ◽  
Vol 32 (Supplement_1) ◽  
Author(s):  
A Mustafa ◽  
A AlKhabaz ◽  
A A Mallik ◽  
S Kumar
Keyword(s):  
Esophageal Atresia ◽  
Eosinophilic Esophagitis ◽  
Tracheoesophageal Fistula ◽  
Gastroesophageal Junction ◽  
Elimination Diet ◽  
Topical Steroids ◽  
Contrast Study ◽  
Esophageal Dysmotility ◽  
Asymptomatic Children ◽  
Repeat Endoscopy

Abstract Esophageal atresia (EA) with or without tracheoesophageal fistula (TEF) requires early surgical intervention in life, following which symptoms related to esophageal dysmotility, gastroesophageal reflux, and strictures may develop. Eosinophilic esophagitis (EoE) is an immune-mediated disorder presents with vomiting and dysphagia. The appearance of exudates, rings, and furrows during endoscopy and the presence of >15 eosinophils/HPF in biopsy confirm the diagnosis. Treatment options include elimination diet, proton pump inhibitors (PPI), and topical steroids. We report a full-term male who had EA and TEF repaired in the second day of life. At 11 months of age, he developed progressive vomiting, contrast study showed esophageal dysmotility with slow emptying and no stricture. The initial endoscopy showed a mild nonobstructive narrowing 5 cm above the gastroesophageal junction, below which the mucosa was erythematous, biopsies showed >25 eosinophils/HPF. Started treatment with PPI and endoscopy repeated three months later showed same endoscopic features and biopsies showed >45 eosinophils/HPF. Allergy testing was positive for milk, wheat, oat, and rice. Topical steroids and elimination diet were added to PPI. At 32 months of age, the child was asymptomatic, not on elimination diet and treatment was stopped. Repeat endoscopy showed longitudinal furrows and erosions of the lower esophagus, no stricture, and biopsies showed >15 eosinophils/HPF. Steroids, PPI, and elimination diet were all resumed. Despite moderate esophagitis due to EoE and poor compliance to treatment, our patient shows normal growth, development, and symptoms resolution. Asymptomatic children with repaired EA and treated EoE may require close follow-up and repeat endoscopy.

Immunohistochemical study of S-100 protein and neuron specific enolase (NSE) in melanocytes and the related tumors

1986 ◽  
Vol 80 (2) ◽  
pp. 215-223 ◽  
Author(s):  
Hiroshi Hachisuka ◽  
Fumino Sakamoto ◽  
Hirofumi Nomura ◽  
Osamu Mori ◽  
Yoichiro Sasai
Keyword(s):  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
S 100
Sign in / Sign up

Export Citation Format

Share Document