Subepithelial neuroendocrine cells and carcinoid tumours of the human small intestine and appendix. A comparative immunohistochemical study with regard to serotonin, neuron-specific enolase and S-100 protein reactivity

1986 ◽  
Vol 148 (2) ◽  
pp. 141-147 ◽  
Author(s):  
Monalill Lundqvist ◽  
Erik Wilander
Keyword(s):  
Small Intestine ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Neuroendocrine Cells ◽  
Carcinoid Tumours ◽  
Human Small Intestine ◽  
S 100

Neuron-specific enolase and S-100 protein immunohistochemistry for defining the structure and topographical relationship of the different enteric nerve plexuses in the small intestine of the pig

1989 ◽  
Vol 256 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Dietrich W. Scheuermann ◽  
Werner Stach ◽  
Jean-Pierre Timmermans ◽  
Dirk Adriaensen ◽  
Marie H. A. De Groodt-Lasseel
Keyword(s):  
Small Intestine ◽  
Neuron Specific Enolase ◽  
S 100 ◽  
Relationship Of

scholarly journals An Immunohistochemical Study of Three Equine Pulmonary Granular Cell Tumors

1995 ◽  
Vol 32 (6) ◽  
pp. 730-734 ◽  
Author(s):  
P. R. Bouchard ◽  
C. H. Fortna ◽  
P. H. Rowland ◽  
R. M. Lewis
Keyword(s):  
Neural Crest ◽  
Granular Cell Tumor ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
Cell Tumor ◽  
Human Beings ◽  
S 100 ◽  
Ultrastructural Characteristics ◽  
Leu 7

Granular cell tumor (GCT) is a morphologic designation for tumors of varied histogenesis. Most GCTs in human beings are derived from Schwann cells, and rat meningeal GCTs are believed to originate in the neural crest. Three equine pulmonary GCTs from aged horses were studied immunohistochemically with primary antibodies directed against vimentin, cytokeratins (AE1/AE3), S-100, Leu 7, desmin, and neuron-specific enolase (NSE) using a steptavidin–biotin procedure. All three tumors stained similarly with strong and diffuse staining of neoplastic cells for vimentin and S-100 and negative staining with all other antibodies. On the basis of the immunohistochemical results and the previously described histologic and ultrastructural characteristics, equine pulmonary GCT is designated as neural crest and possibly Schwann cell derived, similar to GCT in rats and human beings.

scholarly journals Intramural Ganglion Structures in Esophageal Atresia: A Morphologic and Immunohistochemical Study

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Biagio Zuccarello ◽  
Antonella Spada ◽  
Nunzio Turiaco ◽  
Daniela Villari ◽  
Saveria Parisi ◽  
...  
Keyword(s):  
Esophageal Atresia ◽  
Nervous Tissue ◽  
Chromogranin A ◽  
Tracheoesophageal Fistula ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Esophageal Wall ◽  
Motor Disorders ◽  
Esophageal Dysmotility ◽  
S 100

Introduction and Aim. Disorders of esophageal motility causing dysphagia and gastroesophageal reflux are frequent in survivors to esophageal atresia (EA) and distal tracheoesophageal fistula (TEF). The aim of the present study was to investigate the histologic and immunohistochemical features in both esophageal atretic segments to further understand the nature of the motor disorders observed in these patients.Material and Methods. Esophageal specimens from 12 newborns with EA/TEF and 5 newborns dead of unrelated causes were examined. The specimens were fixed in 5% buffered formalin, included in paraffin and cut in 5 micron sections that were stained with hematoxilin and eosin (H and E), and immunohistochemical stainings for Actin, S-100 protein, Neurofilament, Neuron-Specific-Enolase, Chromogranin A and Peripherin were evaluated under the microscope.Results. In controls, the distribution of the neural elements was rather homogenous at both levels of the esophagus. In contrast, the atretic segments showed quantitative and qualitative differences between them with sparser nervous tissue in the distal one in comparison with the proximal one and with controls.Conclusions. These results further support the assumption that histomorphological alterations of the muscular and nervous elements within the esophageal wall might contribute to esophageal dysmotility in patients surviving neonatal operations for EA/TEF.

Eimeria Organisms Develop in the Epithelial Cells of Equine Small Intestine

2002 ◽  
Vol 39 (4) ◽  
pp. 505-508 ◽  
Author(s):  
K. Hirayama ◽  
M. Okamoto ◽  
T. Sako ◽  
K. Kihara ◽  
K. Okai ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Host Cell ◽  
Lamina Propria ◽  
Smooth Muscle Actin ◽  
Eimeria Species ◽  
Neuron Specific Enolase ◽  
Neuroendocrine Cells ◽  
Host Cells

Histopathologic and immunohistochemical examinations were performed to determine the origin of host cells parasitized by Eimeria in the small intestines collected from five foals. Eimeria organisms at various stages (mainly microgametes and macrogametes) were frequently found in the cytoplasm of hypertrophied host cells in the lamina propria at the tips of villi of the jejunum and ileum. The cytoplasm of the host cell was immunohistochemically positive for cytokeratin AE1/AE3 and cytokeratin 13 and was negative for vimentin, desmin, α-smooth muscle actin, chromogranin A, neuron-specific enolase, and factor VIII. The host cells parasitized by Eimeria species had the immunostaining characteristics of epithelial cells but not of mesenchymal cells, endothelial cells of lacteals or capillaries, smooth muscle cells or neuroendocrine cells. These results suggest that the host cell of Eimeria species is possibly derived from intestinal epithelial cells and then displaced into the lamina propria of the small intestine.

scholarly journals Cutaneous Plasmacytomas in Dogs: A Morphologic and Immunohistochemical Study

1989 ◽  
Vol 26 (3) ◽  
pp. 216-221 ◽  
Author(s):  
K. E. Baer ◽  
A. K. Patnaik ◽  
S. R. Gilbertson ◽  
A. I. Hurvitz
Keyword(s):  
Cellular Differentiation ◽  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Benign Neoplasms ◽  
Round Cell ◽  
Immunohistochemical Markers ◽  
S 100 ◽  
Round Cell Tumors

Forty-nine cutaneous plasmacytomas in 46 dogs were studied. Tumors occurred at solitary sites in middle-aged to old dogs (mean age, 9.7 years) and most commonly involved the skin of the digits, lips, and ears. Initial diagnosis was made on the basis of light microscopic morphologic findings. Tumors were graded according to the extent of cellular differentiation and immunoreactivity to a panel of immunohistochemical markers (cytokeratins, canine IgG F[ab]2, neurofilament, neuron-specific enolase, S-100 protein, and vimentin). Immunoreactivity was limited to antibodies directed at canine IgG F(ab)2, and vimentin. Vimentin immunoreactivity was usually greater than that of canine IgG F(ab)2, but there was no correlation between immunoreactivity and histologic grade of the tumors. Thirty-six of 39 dogs (92.3%) followed (mean follow-up, 13 months) were cured by surgical excision. The results of this study indicate that canine cutaneous plasmacytomas are benign neoplasms that should be included in the differential diagnosis of cutaneous round cell tumors in dogs.

Granular Cell Tumor: An Immunohistochemical Study

1994 ◽  
Vol 80 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Antonio Cavaliere ◽  
Angelo Sidoni ◽  
Ivana Ferri ◽  
Brunangelo Falini
Keyword(s):  
Granular Cell Tumor ◽  
Immunohistochemical Study ◽  
Recent Observation ◽  
Neuron Specific Enolase ◽  
Benign Neoplasm ◽  
Granular Cell ◽  
Cell Tumor ◽  
Ultrastructural Studies ◽  
S 100 ◽  
Alpha 1 Antitrypsin

Aims and background Granular cell tumor, usually a benign neoplasm, has been the object of many studies because of its uncertain histogenesis and based on many immunohistochemical and ultrastructural studies it has been suggested that it originates from the Schwann cell. Our recent observation that granular cell tumor is positive with PG-M1, a new anti-macrophage monoclonal antibody, led us to further investigate the immunophenotypic profile of the tumor. Study design We studied 11 granular cell tumors using a panel of 20 antibodies, 13 monoclonal and 7 polyclonal. Results The immunohistochemical study showed in all cases a constant diffuse positivity for S-100 protein, neuron-specific enolase, vimentin, KP1 and PG-M1, as well as occasional and focal positivity for alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme. Conclusions The immunophenotypic profile constantly observed could be the expression, on one hand, of the neuroectodermic nature of the neoplasm, proven by positivity for S-100 protein, neuron specific enolase and vimentin, and on the other could be the expression of the phagocytic activity of the tumor cell, proven by positivity for KP1 and PG-M1 antibodies and also by the presence of numerous phagolysosomes.

Immunohistochemical study of S-100 protein and neuron specific enolase (NSE) in melanocytes and the related tumors

1986 ◽  
Vol 80 (2) ◽  
pp. 215-223 ◽  
Author(s):  
Hiroshi Hachisuka ◽  
Fumino Sakamoto ◽  
Hirofumi Nomura ◽  
Osamu Mori ◽  
Yoichiro Sasai
Keyword(s):  
Immunohistochemical Study ◽  
Neuron Specific Enolase ◽  
S 100
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