scholarly journals Schwann Cells Overexpressing FGF-2 Alone or Combined with Manual Stimulation Do Not Promote Functional Recovery after Facial Nerve Injury

2009 ◽  
Vol 2009 ◽  
pp. 1-11 ◽  
Author(s):  
Kirsten Haastert ◽  
Maria Grosheva ◽  
Srebrina K. Angelova ◽  
Orlando Guntinas-Lichius ◽  
Emmanouil Skouras ◽  
...  
Keyword(s):  
Facial Nerve ◽  
Schwann Cells ◽  
Function Analysis ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Nerve Transection ◽  
Motor Endplates ◽  
Adult Rat ◽  
Manual Stimulation ◽  
Facial Nerves

Purpose. To determine whether transplantation of Schwann cells (SCs) overexpressing different isoforms of fibroblast growth factor 2 (FGF-2) combined with manual stimulation (MS) of vibrissal muscles improves recovery after facial nerve transection in adult rat.Procedures. Transected facial nerves were entubulated with collagen alone or collagen plus naïve SCs or transfected SCs. Half of the rats received daily MS. Collateral branching was quantified from motoneuron counts after retrograde labeling from 3 facial nerve branches. Quality assessment of endplate reinnervation was combined with video-based vibrissal function analysis.Results. There was no difference in the extent of collateral axonal branching. The proportion of polyinnervated motor endplates for either naïve SCs or FGF-2 over-expressing SCs was identical. Postoperative MS also failed to improve recovery.Conclusions. Neither FGF-2 isoform changed the extent of collateral branching or polyinnervation of motor endplates; furthermore, this motoneuron response could not be overridden by MS.

scholarly journals S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

2008 ◽  
Vol 23 (6) ◽  
pp. 555-560 ◽  
Author(s):  
Tatiana Duobles ◽  
Thais de Sousa Lima ◽  
Beatriz de Freitas Azevedo Levy ◽  
Gerson Chadi
Keyword(s):  
Growth Factor ◽  
Sciatic Nerve ◽  
Fibroblast Growth Factor ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Satellite Cells ◽  
Peripheral Nerves ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Cultured Schwann Cells

PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

Expression of interleukin-6 and its receptor in the sciatic nerve and cultured Schwann cells: relation to 18-kD fibroblast growth factor-2

2000 ◽  
Vol 885 (2) ◽  
pp. 172-181 ◽  
Author(s):  
Claudia Grothe ◽  
Klaus Heese ◽  
Christof Meisinger ◽  
Konstantin Wewetzer ◽  
Dieter Kunz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sciatic Nerve ◽  
Fibroblast Growth Factor ◽  
Schwann Cells ◽  
Interleukin 6 ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Cultured Schwann Cells

Bone marrow-derived mesenchymal stem cell transplantation does not improve quality of muscle reinnervation or recovery of motor function after facial nerve transection in rats

2008 ◽  
Vol 389 (7) ◽  
Author(s):  
Maria Grosheva ◽  
Orlando Guntinas-Lichius ◽  
Stephan Arnhold ◽  
Emmanouil Skouras ◽  
Stefanie Kuerten ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Facial Nerve ◽  
Nerve Repair ◽  
Control Treatment ◽  
Lesion Site ◽  
Nerve Reconstruction ◽  
Mesenchymal Stem Cell Transplantation ◽  
Motor Endplates ◽  
Manual Stimulation ◽  
Stimulation Of

AbstractRecently, we devised and validated a novel strategy in rats to improve the outcome of facial nerve reconstruction by daily manual stimulation of the target muscles. The treatment resulted in full recovery of facial movements (whisking), which was achieved by reducing the proportion of pathologically polyinnervated motor endplates. Here, we posed whether manual stimulation could also be beneficial after a surgical procedure potentially useful for treatment of large peripheral nerve defects, i.e., entubulation of the transected facial nerve in a conduit filled with suspension of isogeneic bone marrow-derived mesenchymal stem cells (BM-MSCs) in collagen. Compared to control treatment with collagen only, entubulation with BM-MSCs failed to decrease the extent of collateral axonal branching at the lesion site and did not improve functional recovery. Post-operative manual stimulation of vibrissal muscles also failed to promote a better recovery following entubulation with BM-MSCs. We suggest that BM-MSCs promote excessive trophic support for regenerating axons which, in turn, results in excessive collateral branching at the lesion site and extensive polyinnervation of the motor endplates. Furthermore, such deleterious effects cannot be overridden by manual stimulation. We conclude that entubulation with BM-MSCs is not beneficial for facial nerve repair.

Fibroblast growth factor-2 and its receptor expression in proliferating precursor cells of the subventricular zone in the adult rat brain

2008 ◽  
Vol 447 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Monica Frinchi ◽  
Alessandra Bonomo ◽  
Angela Trovato-Salinaro ◽  
Daniele F. Condorelli ◽  
Kjell Fuxe ◽  
...  
Keyword(s):  
Growth Factor ◽  
Rat Brain ◽  
Fibroblast Growth Factor ◽  
Subventricular Zone ◽  
Precursor Cells ◽  
Receptor Expression ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Adult Rat ◽  
Adult Rat Brain

scholarly journals Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Eudes Euler de Souza Lucena ◽  
Fausto Pierdoná Guzen ◽  
José Rodolfo Lopes de Paiva Cavalcanti ◽  
Maria Jocileide de Medeiros Marinho ◽  
Wogelsanger Oliveira Pereira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Growth Factor ◽  
Facial Nerve ◽  
Fibroblast Growth Factor ◽  
Conditioned Medium ◽  
Nerve Fibers ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Cells Cultured

A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), proteinβ-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2,β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.

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