Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

2006 ◽  
Vol 18 (15) ◽  
pp. 3381-3384 ◽  
Author(s):  
Honghao Sun ◽  
Anne Marie Scharff-Poulsen ◽  
Hong Gu ◽  
Kristoffer Almdal
Keyword(s):  
Fluorescent Dyes ◽  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

Synthesis and characterization of hybrid matrix with encapsulated organic sensing dyes for pH sensing application

2014 ◽  
Vol 20 (6) ◽  
pp. 4408-4414 ◽  
Author(s):  
Shumaila Islam ◽  
Rosly Rahman ◽  
Zulkafli Othaman ◽  
Saira Riaz ◽  
Shahzad Naseem
Keyword(s):  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Hybrid Matrix ◽  
Sensing Application

scholarly journals Ratiometric pH Sensing and Imaging in Living Cells with Dual-Emission Semiconductor Polymer Dots

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2923
Author(s):  
Piaopiao Chen ◽  
Iqra Ilyas ◽  
Su He ◽  
Yichen Xing ◽  
Zhigang Jin ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescein Isothiocyanate ◽  
Water Soluble ◽  
Ph Sensing ◽  
Dual Emission ◽  
Polymer Dots ◽  
Ratiometric Ph Sensing

Polymer dots (Pdots) represent newly developed semiconductor polymer nanoparticles and exhibit excellent characteristics as fluorescent probes. To improve the sensitivity and biocompatibility of Pdots ratiometric pH biosensors, we synthesized 3 types of water-soluble Pdots: Pdots-PF, Pdots-PP, and Pdots-PPF by different combinations of fluorescent dyes poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadazole)] (PFBT), and fluorescein isothiocyanate (FITC). We found that Pdots-PPF exhibits optimal performance on pH sensing. PFO and FITC in Pdots-PPF produce pH-insensitive (λ = 439 nm) and pH-sensitive (λ = 517 nm) fluorescence respectively upon a single excitation at 380 nm wavelength, which enables Pdots-PPF ratiometric pH sensing ability. Förster resonance energy transfer (FRET) together with the use of PFBT amplify the FITC signal, which enables Pdots-PPF robust sensitivity to pH. The emission intensity ratio (I517/I439) of Pdots-PPF changes linearly as a function of pH within the range of pH 3.0 to 8.0. Pdots-PPF also possesses desirable reversibility and stability in pH measurement. More importantly, Pdots-PPF was successfully used for cell imaging in Hela cells, exhibiting effective cellular uptake and low cytotoxicity. Our study suggests the promising potential of Pdots-PPF as an in vivo biomarker.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF AN AMIDOXIME COMPOUND WITH BENZIMIDAZOLE MOIETY AND PH SENSING BEHAVIOR UNDER BASIC CONDITION IN METHANOL AND DMSO

2018 ◽  
Vol 63 (3) ◽  
pp. 4047-4050
Author(s):  
Mohammad Nahidur Rahman ◽  
Rina Rani Bhowmick ◽  
Mohammad Nurnabi ◽  
Ashraful Islam Molla
Keyword(s):  
Synthesis And Characterization ◽  
Ph Sensing

scholarly journals Characterization of PEBBLEs as a Tool for Real-Time Measurement ofDictyostelium discoideumEndosomal pH

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Everett Moding ◽  
Jessica Hellyer ◽  
Kevin Rank ◽  
Phoebe Lostroh ◽  
Murphy Brasuel
Keyword(s):  
Real Time ◽  
Fluorescent Dyes ◽  
Ion Concentration ◽  
Ph Sensing ◽  
Cellular Mechanisms ◽  
Real Time Measurement ◽  
Endosomal Ph ◽  
Camp Stimulation

The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication. Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime moldDictyostelium discoideum (D. discoideum). Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH insideD. discoideumendosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes ofD. discoideumamoebae.

Synthesis and characterization of fluorescent dyes-magnetic nanoparticles for bioimaging applications

2012 ◽  
Author(s):  
Swee Kuan Yen ◽  
Dominik Jańczewski ◽  
Surani Bin Dolmanan ◽  
Tripathy Sudhiranjan ◽  
Subramanian T. Selvan
Keyword(s):  
Magnetic Nanoparticles ◽  
Fluorescent Dyes ◽  
Synthesis And Characterization

scholarly journals Benzothiadiazole oligoene fatty acids: fluorescent dyes with large Stokes shifts

2016 ◽  
Vol 12 ◽  
pp. 2739-2747 ◽  
Author(s):  
Lukas J Patalag ◽  
Daniel B Werz
Keyword(s):  
Fatty Acids ◽  
Chain Length ◽  
Fluorescent Dyes ◽  
Quantum Yields ◽  
Synthesis And Characterization ◽  
Absorption Properties ◽  
Fluorescence Quantum Yields ◽  
Degree Of Unsaturation ◽  
Stokes Shifts

Herein, we report on the synthesis and characterization of novel fluorescent fatty acids with large Stokes shifts. Three examples consisting of the same number of carbon atoms and thus of similar chain length are presented differing in their degree of unsaturation. As major fluorogenic contributor at the terminus benzo[c][1,2,5]thiadiazole was used. Respective syntheses based on Wittig reactions followed by iodine-mediated isomerization are presented. The absorption properties are modulated by the number of conjugated C=C double bonds of the oligoene chain ranging from one to three. Large Stokes shifts of about 4900–5700 cm−1 and fluorescence quantum yields of up to 0.44 were observed.

scholarly journals Synthesis and characterization of fluorescent dyes and their applications for the enhancement of growth rate of Chlorella vulgaris

2018 ◽  
Vol 158 ◽  
pp. 142-150 ◽  
Author(s):  
Hyeyoun Jang ◽  
Jin Woong Namgoong ◽  
Min-Gyu Sung ◽  
Yongkeun Chang ◽  
Jae Pil Kim
Keyword(s):  
Growth Rate ◽  
Chlorella Vulgaris ◽  
Fluorescent Dyes ◽  
Synthesis And Characterization

Synthesis and Characterization of the Three Dicyclopentapyrenes

1996 ◽  
Vol 61 (10) ◽  
pp. 3572-3572
Author(s):  
Lawrence T. Scott ◽  
Atena Necula
Keyword(s):  
Synthesis And Characterization
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