scholarly journals Standardisation Study of Kwatha Curnas

2004 ◽  
Vol 1 (5) ◽  
pp. 251-255
Author(s):  
M. K. Santosh ◽  
D. Shaila ◽  
I. Sanjeeva Rao
Keyword(s):  
High Performance ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Organic Analysis ◽  
Hplc Fingerprint ◽  
Visible Spectrophotometry ◽  
Water Solvent ◽  
Mother And Child ◽  
Uv Visible ◽  
Ethanol Extracts

The present paper deals with the standardization of kwatha curnas such as dhanyapanchak kwatha curna, guduchyadigana kwatha curna and stanyajanankashaya curna. These are the important Ayurvedic formulations used for peri-natal care of mother and child health. Standardization of kwatha curnas were achieved by physico-chemical analysis, qualitative inorganic and organic analysis, thin layer chromatography (TLC), UV- visible spectrophotometry and high performance liquid chromatographic (HPLC) fingerprint studies. TLC study of kwatha curnas was carried out in Ethyl acetate: Methanol: Water solvent system. Ethanol extracts of kwatha curnas were used for UV- visible spectrophotometry and qualitative HPLC fingerprint study.

scholarly journals Standardization Study of Ghritas

2004 ◽  
Vol 1 (3) ◽  
pp. 151-157 ◽  
Author(s):  
D. Shaila ◽  
M. K. Santosh ◽  
T. Chandrakumar ◽  
I. Sanjeeva Rao
Keyword(s):  
Qualitative Analysis ◽  
High Performance ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Hplc Fingerprint ◽  
Visible Spectrophotometry ◽  
Mother And Child ◽  
Hexane Extracts ◽  
Uv Visible ◽  
Liquid Chromatographic

The standardization of ghritas such as amritaprasa ghrita, brahmi ghrita, chagalyadi ghrita and phala ghrita has been studied. These ghritas are the important Ayurvedic formulations used for peri-natal care of mother and child health. Standardization of ghritas were achieved by organoleptic study, physico-chemical analysis, qualitative analysis, thin layer chromatography (TLC), UV - visible spectrophotometry and high performance liquid chromatographic (HPLC) fingerprint studies. Qualitative analysis of alcoholic extracts of all the four ghritas shows the presence of glycosides and hexane extracts shows the presence of glycosides and steroids. TLC study of ghritas was carried out in toulene-ethyl acetate solvent system. Hexane extracts of ghritas were used for UV- visible spectrophotometry and qualitative HPLC fingerprint study.

scholarly journals High-Performance Liquid Chromatographic Fingerprint for Quality Control of Terminalia arjuna Containing Ayurvedic churna Formulation

2009 ◽  
Vol 92 (4) ◽  
pp. 1016-1020 ◽  
Author(s):  
Sohan S Chitlange ◽  
Prajakta S Kulkarni ◽  
Dada Patil ◽  
Bhushan Patwardhan ◽  
Rabindra K Nanda
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Raw Materials ◽  
High Performance Liquid Chromatographic ◽  
Raw Material ◽  
Terminalia Arjuna ◽  
Hplc Fingerprint ◽  
Ayurvedic Drugs ◽  
Quality Control Tool ◽  
Liquid Chromatographic

Abstract Because Ayurvedic herbal preparations contain a myriad of compounds in complex matrixes, it is difficult to establish quality control standards for raw materials and to standardize finished Ayurvedic drugs. A novel, accurate, and valid fingerprint method was developed using HPLC for quality control of a traditional Ayurvedic Arjuna churna formulation, which is used as a cardiotonic drug. Comprehensive comparison of chromatograms of standardized formulation of Arjuna churna and marketed formulations revealed eight characteristic peaks in chromatograms, which unambiguously confirmed the presence of authentic raw material used in the formulation on the basis of their retention time values and UV data. An HPLC fingerprint was also developed for total sapogenins present in Terminalia arjuna. The six common peaks observed in chromatograms of isolated sapogenins, standardized formulations, and marketed formulations can serve as a quality control tool for qualitative estimation of total saponin glycosides present in an Arjuna churna formulation.

scholarly journals Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Wangping Deng ◽  
Tongtong Xu ◽  
Min Yang ◽  
Yajun Cui ◽  
De-an Guo
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Chromatographic Technique ◽  
Hplc Fingerprint ◽  
Solvent Flow Rate ◽  
Principle Components Analysis ◽  
Penthorum Chinense

A high performance liquid chromatographic (HPLC) fingerprint is commonly used for quality consistency evaluation of herbal medicines. Recently, an improved chromatographic technique resulted in ultra high performance liquid chromatography (UHPLC), which could provide higher resolution in less time under higher pressure using finer particles (less than 2μm) of stationary phase. A simple and sensitive method was developed and validated for fingerprint analysis of Penthorum chinense Pursh (PC), with the simultaneous determination of seven components using UPLC coupled with a diode-array detector (DAD). It took less than 20 min for analysis of one sample. Both similarity analysis and principle components analysis (PCA) were employed to evaluate the quality consistency of 17 sample batches. The analysis was performed on a Waters ACQUITY UPLC HSS T3 (2.1 × 150 mm, 1.7 μm) column, which was maintained at 45°C and the eluents were monitored with DAD at 270 nm. A gradient elution with acetonitrile and water containing 0.075% phosphoric acid was used. The solvent flow rate was 0.4 mL/min. Standard calibration curves showed good linear behavior (R2>0.9994) in the range of 0.20-337.05 μg/mL. Acceptable repeatability (RSD<0.61%), reproducibility (RSD<2.72%), stability (RSD<1.59%) and recovery in the range of 94.7%-102.9% were obtained (precision and accuracy). The validated method was successfully applied to evaluate the quality of 21 samples of PC.

Isomerization of Retinyl Palmitate Using Conventional Lipid Extraction Solvents

1983 ◽  
Vol 66 (3) ◽  
pp. 746-750
Author(s):  
Mary C Mulry ◽  
Ronald H Schmidt ◽  
James R Kirk
Keyword(s):  
High Performance ◽  
Methylene Chloride ◽  
Lipid Extraction ◽  
Chlorinated Solvents ◽  
Solvent System ◽  
Retinyl Palmitate ◽  
Fluorometric Detection ◽  
Butyl Ether ◽  
Water Solvent ◽  
Extraction Solvents

Abstract The use or a chlorotorm-ethanol-water solvent system for the direct extraction of retinyl palmitate isomers from fortified food products was previously shown to be unsuitable because significant isomerization of all-trans-retinyl palmitate occurred during the extraction. This study investigated the extent of isomerization of retinyl palmitate in various extraction solvents when subjected to gold fluorescent laboratory light. Purified solutions of all-trans-retinyl palmitate in hexane were diluted with methyl t-butyl ether, hexane, methylene chloride, and stabilized chloroform and subjected to gold fluorescent laboratory light for 2, 4, and 6.5 h. Similar solutions were subjected to light or kept in the dark for 3.5 h. All-trans-, 9-cis-, and 13-cis-retinyl palmitate esters in the solutions were determined by using normal phase high performance liquid chromatography with fluorometric detection. Results demonstrated a noticeable increase in the 9-cis-retinyl palmitate concentration and a corresponding decrease in all-trans-retinyl palmitate concentration with time, in chloroform and methylene chloride compared with hexane. Chlorinated solvents in the absence of light did not promote isomerization of retinyl palmitate. Use of chlorinated solvents for the extraction of vitamin A esters should be avoided because they promote isomerization of retinyl palmitate when subjected to light, including gold fluorescent laboratory light.

High Performance Liquid Chromatographic Determination of Minor Saccharides in Corn Sugar: Collaborative Study

1982 ◽  
Vol 65 (6) ◽  
pp. 1366-1369
Author(s):  
C Earl Engel ◽  
Philip M Olinger ◽  
Raffaele Bernetti ◽  
◽  
K M Brobst ◽  
...  
Keyword(s):  
High Performance ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Mean Values ◽  
Water Solvent ◽  
Coefficients Of Variation ◽  
Aoac Method

Abstract The official first action AOAC method for quantitative determination of saccharides in corn syrup by liquid chromatography was reinvestigated to expand its scope to products containing dextrose in excess of 98%. Because of the low levels of saccharides other than dextrose (DP1), separations are carried out only of the DP1, the disaccharides (DP2), and the tri- and higher saccharides (DP3+) on an Aminex Q15-S, calcium form, cation exchange column. Concentrations of solids in water solvent injected into the liquid chromatograph are higher than those in the AOAC method to increase precision of analysis of the minor saccharides. All carbohydrate components in solution are eluted from the column; therefore, loss of refractive index detector linearity with respect to dextrose is compensated by use of an external maltose (DP2) standard to correct for DP2 and DP3+ response and dextrose is calculated by difference from 100. With mean values of 0.520% DP2 and 0.244% DP3+, the reproducibility and repeatability coefficients of variation are 14.6 and 6.7% for DP2, and 19.3 and 11.9% for DP3+, respectively. Dextrose levels of 99.23% are reproducible within 0.161. The coefficients of variation of the DP2 component are lower by this method than by the AOAC method at levels up to 2% DP2. The method has been adopted official first action.

1,3-Dinitrobenzene – hydroxide ion interactions. Equilibrium constants for σ-complex formation in the dimethylformamide–water solvent system

1981 ◽  
Vol 59 (22) ◽  
pp. 3168-3176 ◽  
Author(s):  
Erwin Buncel ◽  
Allen W. Zabel
Keyword(s):  
Equilibrium Constants ◽  
Solvent System ◽  
Medium Composition ◽  
Hydroxide Ion ◽  
Interacting Species ◽  
Ion Interactions ◽  
Water Solvent ◽  
Constant Measurement ◽  
Uv Visible ◽  
Solvent Isotope

The results of a uv–visible spectroscopic study of the interaction of 1,3-dinitrobenzene (DNB) with hydroxide ion in aqueous dimethylformamide (DMF) media are reported. Formation of several spectral species has been discerned, depending on factors such as the relative concentrations of the reactants, the time scale of the experiments, and the medium composition. The principal interaction is formation of the 1-hydroxy-2,4-dinitrocyclohexadienate anion. The molar absorptivities of this σ-complex, and the equilibrium constants (Ke) for its formation, have been determined as a function of medium composition. Values of Ke increase from 7.5 M−1 to 3 × 105M−1 as the DMF content of the medium is changed from 57.4 to 95.8 mol% DMF. A solvent isotope effect, Ke(D2O)/Ke(H2O), of ca. 0.4 has been observed. Structural and medium effects in this and related systems are evaluated. Correlations between log Ke and H− are examined using the stoichiometric as well as the calculated free hydroxide ion concentrations. Attention is drawn to an important condition for equilibrium constant measurement in systems where one of the interacting species contributes significantly to the overall basicity, or to another property of the medium.

High Performance Liquid Chromatographic Determination of Bifidobacterium bifidum Growth Factors in Human Milk

1983 ◽  
Vol 66 (1) ◽  
pp. 135-139
Author(s):  
Samy H Ashoor ◽  
Woodrow C Monte
Keyword(s):  
Growth Factors ◽  
Human Milk ◽  
High Performance ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Bifidobacterium Bifidum ◽  
Liquid Chromatographic

Abstract An isocratic high performance liquid chromatography (HPLC) method has been developed for the determination of Bifidobacterium bifidum growth factors in human milk. The method involves the gradual addition of 3 volumes of ethanol to the milk sample, filtration, and analysis of the growth factors in the filtrate by HPLC. The HPLC system consisted of a carbohydrate analysis column, a water-acetonitrile (70 + 30) solvent system, a flow rate of 1.0 mL/min, and a refractive index detector. The method is simpler and requires less time than the present microbiological method. Moreover, it revealed for the first time the presence of 2 separable growth factors in all human milk samples tested. The HPLC method developed is sensitive and can be used to monitor the type and the amount of growth factors in mothers’ milk during lactation.

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