scholarly journals Standardization Study of Ghritas

2004 ◽  
Vol 1 (3) ◽  
pp. 151-157 ◽  
Author(s):  
D. Shaila ◽  
M. K. Santosh ◽  
T. Chandrakumar ◽  
I. Sanjeeva Rao
Keyword(s):  
Qualitative Analysis ◽  
High Performance ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Hplc Fingerprint ◽  
Visible Spectrophotometry ◽  
Mother And Child ◽  
Hexane Extracts ◽  
Uv Visible ◽  
Liquid Chromatographic

The standardization of ghritas such as amritaprasa ghrita, brahmi ghrita, chagalyadi ghrita and phala ghrita has been studied. These ghritas are the important Ayurvedic formulations used for peri-natal care of mother and child health. Standardization of ghritas were achieved by organoleptic study, physico-chemical analysis, qualitative analysis, thin layer chromatography (TLC), UV - visible spectrophotometry and high performance liquid chromatographic (HPLC) fingerprint studies. Qualitative analysis of alcoholic extracts of all the four ghritas shows the presence of glycosides and hexane extracts shows the presence of glycosides and steroids. TLC study of ghritas was carried out in toulene-ethyl acetate solvent system. Hexane extracts of ghritas were used for UV- visible spectrophotometry and qualitative HPLC fingerprint study.

scholarly journals Standardisation Study of Kwatha Curnas

2004 ◽  
Vol 1 (5) ◽  
pp. 251-255
Author(s):  
M. K. Santosh ◽  
D. Shaila ◽  
I. Sanjeeva Rao
Keyword(s):  
High Performance ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Organic Analysis ◽  
Hplc Fingerprint ◽  
Visible Spectrophotometry ◽  
Water Solvent ◽  
Mother And Child ◽  
Uv Visible ◽  
Ethanol Extracts

The present paper deals with the standardization of kwatha curnas such as dhanyapanchak kwatha curna, guduchyadigana kwatha curna and stanyajanankashaya curna. These are the important Ayurvedic formulations used for peri-natal care of mother and child health. Standardization of kwatha curnas were achieved by physico-chemical analysis, qualitative inorganic and organic analysis, thin layer chromatography (TLC), UV- visible spectrophotometry and high performance liquid chromatographic (HPLC) fingerprint studies. TLC study of kwatha curnas was carried out in Ethyl acetate: Methanol: Water solvent system. Ethanol extracts of kwatha curnas were used for UV- visible spectrophotometry and qualitative HPLC fingerprint study.

scholarly journals High-Performance Liquid Chromatographic Fingerprint for Quality Control of Terminalia arjuna Containing Ayurvedic churna Formulation

2009 ◽  
Vol 92 (4) ◽  
pp. 1016-1020 ◽  
Author(s):  
Sohan S Chitlange ◽  
Prajakta S Kulkarni ◽  
Dada Patil ◽  
Bhushan Patwardhan ◽  
Rabindra K Nanda
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Raw Materials ◽  
High Performance Liquid Chromatographic ◽  
Raw Material ◽  
Terminalia Arjuna ◽  
Hplc Fingerprint ◽  
Ayurvedic Drugs ◽  
Quality Control Tool ◽  
Liquid Chromatographic

Abstract Because Ayurvedic herbal preparations contain a myriad of compounds in complex matrixes, it is difficult to establish quality control standards for raw materials and to standardize finished Ayurvedic drugs. A novel, accurate, and valid fingerprint method was developed using HPLC for quality control of a traditional Ayurvedic Arjuna churna formulation, which is used as a cardiotonic drug. Comprehensive comparison of chromatograms of standardized formulation of Arjuna churna and marketed formulations revealed eight characteristic peaks in chromatograms, which unambiguously confirmed the presence of authentic raw material used in the formulation on the basis of their retention time values and UV data. An HPLC fingerprint was also developed for total sapogenins present in Terminalia arjuna. The six common peaks observed in chromatograms of isolated sapogenins, standardized formulations, and marketed formulations can serve as a quality control tool for qualitative estimation of total saponin glycosides present in an Arjuna churna formulation.

High Performance Liquid Chromatographic Determination of Bifidobacterium bifidum Growth Factors in Human Milk

1983 ◽  
Vol 66 (1) ◽  
pp. 135-139
Author(s):  
Samy H Ashoor ◽  
Woodrow C Monte
Keyword(s):  
Growth Factors ◽  
Human Milk ◽  
High Performance ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Bifidobacterium Bifidum ◽  
Liquid Chromatographic

Abstract An isocratic high performance liquid chromatography (HPLC) method has been developed for the determination of Bifidobacterium bifidum growth factors in human milk. The method involves the gradual addition of 3 volumes of ethanol to the milk sample, filtration, and analysis of the growth factors in the filtrate by HPLC. The HPLC system consisted of a carbohydrate analysis column, a water-acetonitrile (70 + 30) solvent system, a flow rate of 1.0 mL/min, and a refractive index detector. The method is simpler and requires less time than the present microbiological method. Moreover, it revealed for the first time the presence of 2 separable growth factors in all human milk samples tested. The HPLC method developed is sensitive and can be used to monitor the type and the amount of growth factors in mothers’ milk during lactation.

High Performance Liquid Chromatographic Preparation of Alternariol, Alternariol Methyl Ether, and Altenuene

1981 ◽  
Vol 64 (4) ◽  
pp. 950-954
Author(s):  
Fun S Chu ◽  
S Christopher Bennett
Keyword(s):  
High Performance Liquid Chromatography ◽  
Methyl Ether ◽  
High Performance ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Preparative Hplc ◽  
Alternariol Methyl Ether ◽  
Alternaria Mycotoxins ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract A solvent system was developed for the isolation of 3 major Alternaria mycotoxins by high performance liquid chromatography (HPLC). Alternariol (AOH), alternariol methyl ether (AME), and altenuene (ALT) were purified using either a semipreparative or a preparative HPLC column. Gram quantities of pure mycotoxins were obtained in a single preparative HPLC step. Distribution of other individual mycotoxins and metabolites in various fractions obtained from either chromatographic procedure is discussed.

High Performance Liquid Chromatographic Determination of Vitamin A in Margarine, Milk, Partially Skimmed Milk, and Skimmed Milk

1980 ◽  
Vol 63 (4) ◽  
pp. 894-898 ◽  
Author(s):  
James N Thompson ◽  
George Hatina ◽  
William B Maxwell
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Retinyl Palmitate ◽  
Aqueous Alcohol ◽  
Skimmed Milk ◽  
Liquid Chromatographic ◽  
Β Carotene

Abstract Saponification and subsequent evaporation of extracts can cause losses of vitamin A during analysis and thus cause erratic results. These steps were therefore avoided by measuring retinyl palmitate and β-carotene directly in hexane extracts of milk and margarine. Extracts were purified before high performance liquid chromatography(HPLC) by washing with aqueous alcohol. Retinyl palmitate was measured at 325 nm after chromatography on LiChrosorb Si60, 5 μm, using ethyl ether–hexane (2+98) as the solvent system. Milk (2 mL) was shaken with absolute ethanol (5 mL) and hexane (5 mL). Water (3 mL) was added and, after mixing and centrifugation, 100 μL hexane layer was injected. Margarine (5 g) was dissolved in hexane (100 mL). After 5 mL of the hexane solution was washed with 60% ethanol and centrifuged, a 50 μL aliquot was injected. The results of the retinyl palmitate estimation in milk agreed with those obtained by a fluorometric method; the results in margarine agreed with those obtained by saponification, extraction, and HPLC. The coefficients of variation on analysis of 10 replicate samples of milk and margarine were 3 and 4.5%, respectively. The analysis of margarine for vitamin A was completed by measuring β-carotene with a detector set at 453 nm; the coefficient of variation of this measurement was 3% (10 replicates).

High Performance Liquid Chromatographic Determination of Vitamin D in Fortified Milks, Margarine, and Infant Formulas

1982 ◽  
Vol 65 (3) ◽  
pp. 624-631 ◽  
Author(s):  
James N Thompson ◽  
George Hatina ◽  
William B Maxwell ◽  
Suzanne Duval
Keyword(s):  
Vitamin D ◽  
High Performance ◽  
Room Temperature ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Fortified milks were saponified overnight at room temperature with 1% ethanolic pyrogallol and KOH. The digest was extracted with hexane after adding water and ethanol, and the extract was washed consecutively with 5% KOH, water, and 55% aqueous ethanol to remove polar lipids. After evaporation, the residue was first chromatographed on a column of 5 μm silica. A fraction containing vitamin D was collected, evaporated, and rechromatographed on a reverse phase column for the separation and quantitation of vitamins D2 and D3. Recovery was 96-99% and the coefficient of variation was 3% (8 replicates). Infant formula was diluted and then saponified and extracted as in the analysis of milk. Margarine was saponified by shaking overnight with 1% ethanolic pyrogallol and 80% KOH. Water and ethanol were added to the digest before extraction. Extracts from formula and margarine were chromatographed as milk except, before HPLC, the extract was dissolved in isopropanol-hexane (1 + 99) and passed through 5 cm alumina in a Pasteur pipet, and the concentration of isopropanol in the first high performance liquid chromatographic (HPLC) solvent system was halved to improve the separation of vitamin D from other absorbing lipids. Usually several peaks were obtained during the final HPLC analysis, and the identification of vitamins D2 and D3 was less certain than in the analysis of milk. The coefficients of variation for formula and margarine were 6% (5 replicates) and 9% (6 replicates), respectively.

High Performance Liquid Chromatographic Determination of Subsidiary Colors in FD&C Red No. 3

1982 ◽  
Vol 65 (5) ◽  
pp. 1080-1085
Author(s):  
Robert J Calvey ◽  
Allen L Goldberg
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
Total Sample ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Phase Method ◽  
Reverse Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Two rapid, sensitive, reproducible methods that use a Zorbax C-8 reverse phase column and high performance liquid chromatography are described for the determination of the subsidiary colors in FD&C Red No. 3. With the first method, 8 subsidiary colors (fluorescein, 2'-iodofluorescein, 4'-iodofluorescein, 2',5'-diiodofluorescein, 2',7'-diiodofluorescein, 4',- 5'-diiodofluorescein, 2',4',7'-triiodofluorescein, and 2',4',5'-triiodofluorescein) are eluted in a reproducible pattern by increasing the organic nature of a buffered mobile phase. Method 1 is capable of quantitating all the subsidiary colors except 4'-iodofluorescein and 4',5'-diiodofluorescein. If these 2 subsidiary colors are seen, the sample must be run again by method 2, which uses a different program and solvent system to quantitate them. The average recoveries for the 6 subsidiary colors quantitatively determined in FD&C Red No. 3 by method 1 ranged from 96 to 98%. The average recoveries for 4'-iodofluorescein and 4',5'-diiodofluorescein, quantitatively determined in FD&C Red No. 3 by method 2, were 101 and 103%, respectively. The amounts of the 6 subsidiary colors recovered by method 1 were 0.04-7.4% by weight of the total sample. The amounts of the 2 subsidiary colors recovered by method 2 were 0.13-2.6% by weight of the total sample.

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