In situCharacterisation of Living Cells by Raman Spectroscopy

I. Notingher; S. Verrier; H. Romanska; A. E. Bishop; J. M. Polak; L. L. Hench

doi:10.1155/2002/408381

In situCharacterisation of Living Cells by Raman Spectroscopy

Spectroscopy An International Journal ◽

10.1155/2002/408381 ◽

2002 ◽

Vol 16 (2) ◽

pp. 43-51 ◽

Cited By ~ 151

Author(s):

I. Notingher ◽

S. Verrier ◽

H. Romanska ◽

A. E. Bishop ◽

J. M. Polak ◽

...

Keyword(s):

Trypan Blue ◽

Laser Excitation ◽

Living Cells ◽

Fused Silica ◽

Excitation Wavelength ◽

Bioactive Scaffolds ◽

Non Invasive ◽

Invasive Method ◽

45S5 Bioglass

We report the first Raman spectra of individual living and dead cells (MLE-12 line) cultured on bioinert standard poly-L-lysine coated fused silica and on bioactive 45S5 Bioglass®measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to the cells even after 40 minutes irradiation at 115 mW power, as indicated by cell morphology observation and trypan blue viability test. We show that shorter wavelength lasers, 488 nm and 514 nm, cannot be used because they induce damage to the cells at very low laser powers (5 mW) and short irradiation times (5–20 minutes). The most important differences between the spectra of living and dead cells are in the 1530–1700 cm−1range, where the dead cells have strong peaks at 1578 cm−1and 1607 cm−1. Other differences occur around the DNA peak at 1094 cm−1. Our study establishes the feasibility of using the 785 nm laser for anin situreal-time non-invasive method to follow biological events (proliferation, differentiation, cell death, etc.) within individual cells cultured on bioactive scaffolds in their physiologic environment over long periods of time.

Download Full-text

Non-invasive method for in situ estimation of intensity using non-linear effects in ultrasound hyperthermia

Ultrasonics ◽

10.1016/0041-624x(92)90052-n ◽

1992 ◽

Vol 30 (2) ◽

pp. 123-124 ◽

Cited By ~ 1

Author(s):

M.Serhat Özyar ◽

Hayrettin Köymen

Keyword(s):

Non Invasive ◽

Non Linear ◽

Invasive Method ◽

Linear Effects ◽

Ultrasound Hyperthermia

Download Full-text

Combined simultaneous analysis of morphology and fluorescence in situ hybridization (FISH) as novel sensitive non-invasive method for early detection of aneuploid cells in premalignant and malignant oral and laryngeal lesions

Cancer Genetics and Cytogenetics ◽

10.1016/j.cancergencyto.2010.07.098 ◽

2010 ◽

Vol 203 (1) ◽

pp. 90

Author(s):

Luba Trakhtenbrot ◽

Tali Shani ◽

Avraham Hirshberg ◽

Wolf Michael ◽

Gideon Rechavi ◽

...

Keyword(s):

In Situ Hybridization ◽

Early Detection ◽

Fluorescence In Situ Hybridization ◽

Simultaneous Analysis ◽

Non Invasive ◽

Laryngeal Lesions ◽

Invasive Method

Download Full-text

Photosensitizers Used in the Photodynamic Therapy of Rheumatoid Arthritis

International Journal of Molecular Sciences ◽

10.3390/ijms20133339 ◽

2019 ◽

Vol 20 (13) ◽

pp. 3339 ◽

Cited By ~ 10

Author(s):

Manuel Gallardo-Villagrán ◽

David Yannick Leger ◽

Bertrand Liagre ◽

Bruno Therrien

Keyword(s):

Rheumatoid Arthritis ◽

Photodynamic Therapy ◽

Light Irradiation ◽

Excitation Wavelength ◽

Target Tissue ◽

Non Invasive ◽

Different Types ◽

Invasive Method ◽

Promising Treatment ◽

As Solubility

Photodynamic Therapy (PDT) has become one of the most promising treatment against autoimmune diseases, such as rheumatoid arthritis (RA), as well as in the treatment of different types of cancer, since it is a non-invasive method and easy to carry out. The three main ingredients of PDT are light irradiation, oxygen, and a photosensitizer (PS). Light irradiation depends on the type of molecule or compound to be used as a PS. The concentration of O2 fluctuates according to the medium where the target tissue is located and over time, although it is known that it is possible to provide oxygenated species to the treated area through the PS itself. Finally, each PS has its own characteristics, the efficacy of which depends on multiple factors, such as solubility, administration technique, retention time, stability, excitation wavelength, biocompatibility, and clearance, among others. Therefore, it is essential to have a thorough knowledge of the disease to select the best PS for a specific target, such as RA. In this review we will present the PSs used in the last three decades to treat RA under PDT protocol, as well as insights on the relevant strategies.

Download Full-text

Application of FTIR and Raman Spectroscopy to Characterisation of Bioactive Materials and Living Cells

Spectroscopy An International Journal ◽

10.1155/2003/893584 ◽

2003 ◽

Vol 17 (2-3) ◽

pp. 275-288 ◽

Cited By ~ 55

Author(s):

I. Notingher ◽

J. R. Jones ◽

S. Verrier ◽

I. Bisson ◽

P. Embanga ◽

...

Keyword(s):

Raman Spectroscopy ◽

Near Infrared ◽

Cell Damage ◽

Living Cells ◽

Biologically Active ◽

Bioactive Materials ◽

45S5 Bioglass ◽

Ftir And Raman Spectroscopy

Both Fourier Transform Infrared (FTIR) and Raman spectroscopy have been applied to thein vitrocharacterisation of biomaterials, mainly surface reactions leading to the formation of a biologically active hydroxycarbonate apatite (HCA) layer on the sample surface when immersed in simulated body fluids (SBF). The HCA layer indicates the degree of bioactivity of the sample, because it leads to a strong bond between the biomaterial and living tissue. Reflection measurements using FTIR allow quick, non-destructive detection of the HCA layer for solid and powder samples. Due to the low Raman scattering efficiency and low absorption of water in the visible-near infrared region, Raman micro-spectroscopy was successfully used for thein situcharacterisation of 20 and 40µm diameter 45S5 Bioglass®fibres. Thein situcapabilities of the Raman micro-spectrometer have also been extended to the characterisation of living cells attached on bioinert silica and bioactive 45S5 Bioglass®and 58S substrates. Using a high power 785 nm laser, living cells in physiological conditions can be real-time sampled over long periods of time without inducing cell damage and with good signal strength. Cell death can be monitored because it proved to induce strong changes in the Raman signature in the spectral regions 1000–1150 cm–1and 1550–1650 cm–1.

Download Full-text

Optimization of Optical Trapping and Laser Interferometry in Biological Cells

Applied Sciences ◽

10.3390/app10144970 ◽

2020 ◽

Vol 10 (14) ◽

pp. 4970

Author(s):

Yujiro Sugino ◽

Masahiro Ikenaga ◽

Daisuke Mizuno

Keyword(s):

Optical Trapping ◽

Laser Interferometry ◽

Living Cells ◽

Colloidal Probe ◽

Non Invasive ◽

In Situ Calibration ◽

The Stability ◽

Sample Heterogeneity

Optical trapping and laser interferometry enable the non-invasive manipulation of colloids, which can be used to investigate the microscopic mechanics of surrounding media or bound macromolecules. For efficient trapping and precise tracking, the sample media must ideally be homogeneous and quiescent whereas such conditions are usually not satisfied in vivo in living cells. In order to investigate mechanics of the living-cell interior, we introduced (1) the in-situ calibration of optical trapping and laser interferometry, and (2) 3-D feedback control of a sample stage to stably track a colloidal particle. Investigating systematic errors that appear owing to sample heterogeneity and focal offsets of a trapping laser relative to the colloidal probe, we provide several important caveats for conducting precise optical micromanipulation in living cells. On the basis of this study, we further improved the performance of the techniques to be used in cells, by optimizing the position sensitivity of laser interferometry and the stability of the feedback simultaneously.

Download Full-text

Locating an ice-covered Antarctic landfill using ground magnetometry

Antarctic Science ◽

10.1017/s0954102013000953 ◽

2014 ◽

Vol 26 (4) ◽

pp. 361-368 ◽

Cited By ~ 1

Author(s):

Benjamin L. Freidman ◽

Danielle Camenzuli ◽

Mark Lackie

Keyword(s):

Metal Contamination ◽

Cost Effective ◽

Magnetic Anomalies ◽

Material Surface ◽

Magnetic Susceptibilities ◽

North West ◽

Non Invasive ◽

Invasive Method ◽

Antarctic Research

AbstractAt former Antarctic research stations, legacy waste often remains in situ and concealed by ice. Consequently, the location, characteristics and potential environmental impact associated with legacy waste remains poorly documented. This study applies ground magnetometry to map the spatial extent of the landfill at the abandoned Wilkes Station. Magnetic anomalies indicate that the landfill extends north-west to south-east and is close to, and perhaps prograding into, the ocean. The landfill is characterized by large magnetic variations of > 1500 nT with asymmetrical magnetic anomalies which suggest variable orientations of material and random dumping. Magnetic susceptibilities > 0.02SI units beyond the landfill area reveal elevated magnetic properties of the basement geology. However, a contrast in anomaly shape reliably distinguishes large anomalies generated by landfill material. Surface and subsurface melt streams (observed at the shoreline) flowing from the survey area suggest elevated potential for metal contamination of the nearshore and marine environment. The survey demonstrates a cost-effective and non-invasive method for gathering information to guide the clean up of landfills beneath ice.

Download Full-text

A non invasive method to detect stratigraphy, thicknesses and pigment concentration of pictorial multilayers based on EDXRF and vis-RS: in situ applications

Applied Physics A ◽

10.1007/s00339-008-4482-6 ◽

2008 ◽

Vol 92 (1) ◽

pp. 203-210 ◽

Cited By ~ 20

Author(s):

L. Bonizzoni ◽

S. Caglio ◽

A. Galli ◽

G. Poldi

Keyword(s):

Pigment Concentration ◽

Non Invasive ◽

Invasive Method

Download Full-text

In situ detection of salicylate in Ocimum basilicum plant leaves via reverse iontophoresis

Chemical Communications ◽

10.1039/c5cc06909b ◽

2015 ◽

Vol 51 (92) ◽

pp. 16534-16536 ◽

Cited By ~ 12

Author(s):

M. I. González-Sánchez ◽

P. T. Lee ◽

R. H. Guy ◽

R. G. Compton

Keyword(s):

Cyclic Voltammetry ◽

Quantitative Method ◽

Ocimum Basilicum ◽

Plant Leaves ◽

Reverse Iontophoresis ◽

Non Invasive ◽

Screen Printed Electrodes ◽

Invasive Method ◽

In Situ Detection

We report an in situ quantitative method to measure the concentration of salicylates, from intact, living Ocimum basilicum plant leaves. This simple, non-invasive method utilises iontophoresis in combination with cyclic voltammetry at disposable screen-printed electrodes.

Download Full-text

The importance of continuing research during the demolition of architectural wooden architecture on the example of pens from Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka

Budownictwo i Architektura ◽

10.35784/bud-arch.1618 ◽

2015 ◽

Vol 14 (3) ◽

pp. 089-106

Author(s):

Anna Maślak

Keyword(s):

Main Task ◽

Progressive Deterioration ◽

Non Invasive ◽

Conservation Project ◽

Conservation Measures ◽

Invasive Method ◽

The Creation ◽

Facility Construction ◽

Architectural Research

'Olęder' Ethnographic Park in Nieszawka Wielka is Poland's only open-air museum that has been dedicated to construction of the rural, associated with olęder settlement. Currently, its research range covers an area of Lower Vistula Valley. Existing conservation measures, which main task is to protect architectural monuments in situ, however, is not always sufficient or possible. Preserved until today, very few examples of this construction are threatened by the progressive deterioration leading to their elimination from the Vistula landscape. The creation of a museum in the open air and translocation of these precious relics is actually the only chance for their continued survival. As a preliminary point, I will briefly present the characteristics of the 'olęder' settlement in the Lower Vistula Valley and the concept of 'Olęder' Ethnographic Park in Nieszawka Wielka along with attempts to establish a new branch of the Museum. The purpose of this article is to highlight the importance of supporting architectural research during the demolition of wooden architecture on the example of pens in Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka. Initially, the concept of conservation project was discussed, developed on the basis of architectural research conducted before demolition. These studies were conducted using a non-invasive method, making it difficult to access all components and places. Supplementing the research during the demolition shed a new light on conservation issues. The summary of activities related to the translocation will show the last phase of facility construction.

Download Full-text