scholarly journals In situ detection of salicylate in Ocimum basilicum plant leaves via reverse iontophoresis

2015 ◽  
Vol 51 (92) ◽  
pp. 16534-16536 ◽  
Author(s):  
M. I. González-Sánchez ◽  
P. T. Lee ◽  
R. H. Guy ◽  
R. G. Compton
Keyword(s):  
Cyclic Voltammetry ◽  
Quantitative Method ◽  
Ocimum Basilicum ◽  
Plant Leaves ◽  
Reverse Iontophoresis ◽  
Non Invasive ◽  
Screen Printed Electrodes ◽  
Invasive Method ◽  
In Situ Detection

We report an in situ quantitative method to measure the concentration of salicylates, from intact, living Ocimum basilicum plant leaves. This simple, non-invasive method utilises iontophoresis in combination with cyclic voltammetry at disposable screen-printed electrodes.

In situ detection of intermediates from the interaction of dissolved sulfide and manganese oxides with a platinum electrode in aqueous systems

2017 ◽  
Vol 14 (3) ◽  
pp. 178 ◽  
Author(s):  
Yao Luo ◽  
Yougang Shen ◽  
Lihu Liu ◽  
Jun Hong ◽  
Guohong Qiu ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Manganese Oxides ◽  
Platinum Electrode ◽  
Aqueous Systems ◽  
Oxidation Peak ◽  
Peak Current ◽  
Oxidation Peak Current ◽  
Dissolved Sulfide ◽  
In Situ Detection

Environmental contextDissolved sulfide results in soil acidification and subsequent contaminant leaching via oxidation processes, usually involving manganese oxides. In this work, redox processes were monitored in situ by cyclic voltammetry and HS– concentrations were semi-quantitatively determined. The method provides qualitative and semi-quantitative assessment for dissolved sulfide and its oxidation intermediates in aqueous systems. AbstractDissolved sulfide can be oxidised by manganese oxides in supergene environments, while the intermediates including S0, S2O32– and SO32– are easily oxidised by oxygen in air, resulting in some experimental errors in conventional analyses. In this work, the electrochemical behaviours of HS–, S2O32– and SO32– on a platinum electrode were studied by cyclic voltammetry and constant potential electrolysis, and in situ detection of the intermediates was conducted in aqueous systems of HS– and manganese oxides. The results showed that HS– was first oxidised to S0, and then transformed to SO42–. The peak current for the oxidation of HS– to S0 had a positive linear correlation with the used starting HS– concentration. S2O32– and SO32– were directly electrochemically oxidised to SO42–. The oxidation current peak potentials at 0, 0.45 and 0.7V were respectively observed for HS–, S2O32– and SO32– at pH 12.0. Cyclic voltammetry was conducted to monitor the redox processes of HS– and manganese oxides. The oxidation peak current of HS– to S0 decreased, and that of S2O32– to SO42– was observed to increase as the reaction proceeded. The rate of the decrease of the oxidation peak current of HS– indicated that the oxidation activity followed the order of birnessite>todorokite>manganite.

scholarly journals Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

2013 ◽  
Vol 2 (2) ◽  
pp. 157-164 ◽  
Author(s):  
A. Zuzuarregui ◽  
S. Arana ◽  
E. Pérez-Lorenzo ◽  
S. Sánchez-Gómez ◽  
G. Martínez de Tejada ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Polymyxin B ◽  
Device Fabrication ◽  
Self Assembled Monolayer ◽  
Fully Integrated ◽  
Simple Instrument ◽  
Pre Treatment ◽  
In Situ Detection ◽  
Treatment Procedures

Abstract. In this paper an electrochemical endotoxin biosensor consisting of an immobilized lipopolysaccharide (LPS) ligand, polymyxin B (PmB), is presented. Several parameters involved both in the device fabrication and in the detection process were analyzed to optimize the ligand immobilization and the interaction between PmB and LPS, aiming at increasing the sensitivity of the sensor. Different electrochemical pre-treatment procedures as well as the functionalization methods were studied and evaluated. The use of a SAM (self-assembled monolayer) to immobilize PmB and the quantification of the interactions via cyclic voltammetry allowed the development of a robust and simple device for in situ detection of LPS. Thus, the biosensor proposed in this work intends an approach to the demanding needs of the market for an integrated, portable and simple instrument for endotoxin detection.

scholarly journals In situCharacterisation of Living Cells by Raman Spectroscopy

2002 ◽  
Vol 16 (2) ◽  
pp. 43-51 ◽  
Author(s):  
I. Notingher ◽  
S. Verrier ◽  
H. Romanska ◽  
A. E. Bishop ◽  
J. M. Polak ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Laser Excitation ◽  
Living Cells ◽  
Fused Silica ◽  
Excitation Wavelength ◽  
Bioactive Scaffolds ◽  
Non Invasive ◽  
Invasive Method ◽  
45S5 Bioglass

We report the first Raman spectra of individual living and dead cells (MLE-12 line) cultured on bioinert standard poly-L-lysine coated fused silica and on bioactive 45S5 Bioglass®measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to the cells even after 40 minutes irradiation at 115 mW power, as indicated by cell morphology observation and trypan blue viability test. We show that shorter wavelength lasers, 488 nm and 514 nm, cannot be used because they induce damage to the cells at very low laser powers (5 mW) and short irradiation times (5–20 minutes). The most important differences between the spectra of living and dead cells are in the 1530–1700 cm−1range, where the dead cells have strong peaks at 1578 cm−1and 1607 cm−1. Other differences occur around the DNA peak at 1094 cm−1. Our study establishes the feasibility of using the 785 nm laser for anin situreal-time non-invasive method to follow biological events (proliferation, differentiation, cell death, etc.) within individual cells cultured on bioactive scaffolds in their physiologic environment over long periods of time.

Locating an ice-covered Antarctic landfill using ground magnetometry

2014 ◽  
Vol 26 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Benjamin L. Freidman ◽  
Danielle Camenzuli ◽  
Mark Lackie
Keyword(s):  
Metal Contamination ◽  
Cost Effective ◽  
Magnetic Anomalies ◽  
Material Surface ◽  
Magnetic Susceptibilities ◽  
North West ◽  
Non Invasive ◽  
Invasive Method ◽  
Antarctic Research

AbstractAt former Antarctic research stations, legacy waste often remains in situ and concealed by ice. Consequently, the location, characteristics and potential environmental impact associated with legacy waste remains poorly documented. This study applies ground magnetometry to map the spatial extent of the landfill at the abandoned Wilkes Station. Magnetic anomalies indicate that the landfill extends north-west to south-east and is close to, and perhaps prograding into, the ocean. The landfill is characterized by large magnetic variations of > 1500 nT with asymmetrical magnetic anomalies which suggest variable orientations of material and random dumping. Magnetic susceptibilities > 0.02SI units beyond the landfill area reveal elevated magnetic properties of the basement geology. However, a contrast in anomaly shape reliably distinguishes large anomalies generated by landfill material. Surface and subsurface melt streams (observed at the shoreline) flowing from the survey area suggest elevated potential for metal contamination of the nearshore and marine environment. The survey demonstrates a cost-effective and non-invasive method for gathering information to guide the clean up of landfills beneath ice.

scholarly journals The importance of continuing research during the demolition of architectural wooden architecture on the example of pens from Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka

2015 ◽  
Vol 14 (3) ◽  
pp. 089-106
Author(s):  
Anna Maślak
Keyword(s):  
Main Task ◽  
Progressive Deterioration ◽  
Non Invasive ◽  
Conservation Project ◽  
Conservation Measures ◽  
Invasive Method ◽  
The Creation ◽  
Facility Construction ◽  
Architectural Research

'Olęder' Ethnographic Park in Nieszawka Wielka is Poland's only open-air museum that has been dedicated to construction of the rural, associated with olęder settlement. Currently, its research range covers an area of Lower Vistula Valley. Existing conservation measures, which main task is to protect architectural monuments in situ, however, is not always sufficient or possible. Preserved until today, very few examples of this construction are threatened by the progressive deterioration leading to their elimination from the Vistula landscape. The creation of a museum in the open air and translocation of these precious relics is actually the only chance for their continued survival. As a preliminary point, I will briefly present the characteristics of the 'olęder' settlement in the Lower Vistula Valley and the concept of 'Olęder' Ethnographic Park in Nieszawka Wielka along with attempts to establish a new branch of the Museum. The purpose of this article is to highlight the importance of supporting architectural research during the demolition of wooden architecture on the example of pens in Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka. Initially, the concept of conservation project was discussed, developed on the basis of architectural research conducted before demolition. These studies were conducted using a non-invasive method, making it difficult to access all components and places. Supplementing the research during the demolition shed a new light on conservation issues. The summary of activities related to the translocation will show the last phase of facility construction.

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