scholarly journals DNA Histogram Interpretation Based on Statistical Approaches

1997 ◽  
Vol 15 (3) ◽  
pp. 157-173 ◽  
Author(s):  
G. Haroske ◽  
V. Dimmer ◽  
W. Meyer ◽  
K. D. Kunze
Keyword(s):  
Dna Ploidy ◽  
Human Interaction ◽  
Breast Cancers ◽  
Stochastic Sampling ◽  
Stochastic Basis ◽  
The Common ◽  
Error Probabilities ◽  
Dna Measurements ◽  
Dna Histograms ◽  
Dna Histogram

Image cytometric DNA measurements provide data which are most often interpreted as equivalent to the chromosomal ploidy although the chromosomal and the DNA ploidy are not identical. The common link between them is the cell cycle. Therefore, if destined for DNA ploidy interpretations, the DNA cytometry should be performed on a population‐oriented stochastic basis. Using stochastic sampling the data can be interpreted by applying the rules of stochastic processes. A set of statistical methods is given that enables a DNA histogram to be interpreted objectively and without human interaction. These statistics analyse the precision and accuracy of the entire measurement process. They give in error probabilities for accepting a measurement as reliable, for recognition of stemlines, stemline aneuploidy, and for evaluating so‐called rare events. Nearly 300 image cytometric DNA measurements from breast cancers and rat liver imprints examples have been selected to demonstrate the efficiency of the statistics in each step of interpreting DNA histograms.

Flow cytometric analysis of DNA ploidy in primary, metastatic and recurrent breast cancers

2002 ◽  
Author(s):  
Shinichi Tsutsui ◽  
Shinji Ohno ◽  
Shigeru Murakami ◽  
Akemi Kataoka ◽  
Junko Kinoshita ◽  
...  
Keyword(s):  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
Breast Cancers ◽  
Flow Cytometric ◽  
Recurrent Breast

scholarly journals Primary Stromal Breast Sarcoma with Concomitant Contralateral Carcinoma: A Rare Case from Syria

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Rawan Al khudari ◽  
Mohannad Homsi ◽  
Hasan Al zohaily ◽  
Maher S. Saifo
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Rare Case ◽  
Ductal Carcinoma ◽  
Primary Lesion ◽  
Breast Cancers ◽  
Breast Sarcoma ◽  
Common Presentation ◽  
Stromal Sarcoma ◽  
The Common ◽  
Contralateral Carcinoma

Bilateral breast cancers are rare cases encountered and are usually the same type in both sides. Only very few cases were reported to have different histological types of neoplasia involving sarcoma. Moreover, sarcomas rarely originate from the breast as a primary lesion whereas the common presentation is having angiosarcoma following radiotherapy. In this report, we present a rare case of a Syrian 43-year-old woman having two distinct primary lesions in the breasts: invasive ductal carcinoma and contralateral stromal sarcoma.

Relationships of DNA ploidy, S-phase fraction and hormone receptor status to tumor stage in breast cancers detected by population screening

1992 ◽  
Vol 51 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Olle Stål ◽  
Ann Brisfors ◽  
John Carstensen ◽  
Lilianne Ferraud ◽  
Thomas Hatschek ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Dna Ploidy ◽  
Tumor Stage ◽  
Hormone Receptor Status ◽  
S Phase ◽  
Population Screening ◽  
Phase Fraction ◽  
Breast Cancers ◽  
Receptor Status

scholarly journals Correlation between p53 Status, DNA Ploidy, Proliferation Rate and Nuclear Morphology in Breast Cancer. An Image Cytometric Study

1997 ◽  
Vol 15 (2) ◽  
pp. 85-97 ◽  
Author(s):  
Katrin Friedrich ◽  
Volker Dimmer ◽  
Gunter Haroske ◽  
Wolfdietrich Meyer ◽  
Franz Theissig ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumour Cell ◽  
Dna Ploidy ◽  
Image Cytometry ◽  
Proliferation Rate ◽  
Cell Populations ◽  
Nuclear Morphology ◽  
Breast Cancers ◽  
P53 Expression ◽  
High Proliferation Rate

The study was designed to detect differences in the nuclear morphology of tumours and tumour cell populations with different p53 expression in correlation with DNA ploidy and proliferation rate. The paraffin sections from routinely processed samples of 88 breast cancers were immunostained with the monoclonal p53‐antibody DO‐1. After localization and evaluation with a scoring system the sections were destained and stained by the Feulgen method. The nuclei were relocated automatically and measured by means of the image cytometry workstation. Significant differences between the tumours and tumour cell populations with different p53 expression were found in the euploid tumours as well as in the aneuploid tumours and in the breast cancers with a high proliferation rate. The breast cancers with a low immunoreactive score (IRS 1–4) differ from the negative cancers as well as from the cancers with a higher immunoreactive score (IRS 5–12). Evaluating the nuclear populations of the p53 positive cancers, there were differences in the features of the chromatin amount and distribution in the groups of the euploid breast cancers and in cancer with a high proliferation rate. In contrast, the nuclear populations of the aneuploid cancers did not show any differences in their nuclear morphology.The results showed the different impacts of the p53 expression, DNA ploidy and the proliferation rate on the nuclear morphology in breast cancer.

scholarly journals Blocking of EGFR Signaling is a Latent Strategy for the Improvement of Prognosis of HPV Induced Cancer

2020 ◽  
Author(s):  
Jianfa Qiu ◽  
Feifei Hu ◽  
Tingting Shao ◽  
Yuqiang Guo ◽  
Zongmao Dai ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Structure Prediction ◽  
Molecular Mechanisms ◽  
Interaction Network ◽  
Hpv Infection ◽  
Human Interaction ◽  
Hpv E7 ◽  
Significant Difference ◽  
Cancer Types ◽  
The Common

AbstractHuman papillomavirus (HPV) is a dsDNA virus and its high-risk subtypes increase cancer risks. Yet, the mechanism of HPV infection and pathogenesis still remain unclear. Therefore, understanding the molecular mechanisms, and the pathogenesis of HPV are crucial in the prevention of HPV related cancers. In this study, we analyzed cervix squamous cell carcinoma (CESC) and head and neck carcinoma (HNSC) combined data to investigate various HPV induced cancer common feature. We showed that epidermal growth factor receptor (EGFR) was downregulated in HPV positive (HPV+) cancer, and that HPV+ cancer patients exhibited better prognosis than HPV negative (HPV−) cancer patients. Our study also showed that TP53 mutation rate is lower in HPV+ cancer than in HPV− cancer and that TP53 can be modulated by HPV E7 protein. However, there was no significant difference in the expression of wildtype TP53 in both groups. Subsequently, we constructed HPV-human interaction network and found that EGFR is a critical factor. From the network, we also noticed that EGFR is regulated by HPV E7 protein and hsa-miR-944. Moreover, while phosphorylated EGFR is associated with a worse prognosis, EGFR total express level is not significantly correlated with prognosis. This indicates that EGFR activation will induce a worse outcome in HPV+ cancer patients. Further enrichment analysis showed that EGFR downstream pathway and cancer relative pathway are diversely activated in HPV+ cancer and HPV− cancer. In summary, HPV E7 protein downregulates EGFR that downregulates phosphorylated EGFR and inhibit EGFR related pathways which in turn and consequently induce better prognosis.ImportanceAlthough HPV infection has been studied in various cancer types, there are only limited studies that have focused on the common effect of HPV related cancer. Consequently, this study focused on CESC and HNSC, two cancer types with high HPV infection proportion in cohort, thereby, intending to dig out the common effects and mechanisms of HPV+ cancers.Unlike some virus-human interaction prediction studies, the P-HIPSter database provides virus-human protein interaction based on protein structure prediction. Through this data, our interaction network was able to uncover previously unnoticed protein interactions. Our finding revealed that HPV infection caused various gene expression differences, and a great amount of which interact with EGFR, a cancer related gene. Therefore, since EGFR is associated with HPV+ cancer patients’ survival, some FDA proved EGFR inhibitors would be potential anti-HPV drugs.

scholarly journals DNA histogram analysis of human hemopoietic cells

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 245-258
Author(s):  
B Barlogie ◽  
G Spitzer ◽  
JS Hart ◽  
DA Johnston ◽  
T Buchner ◽  
...  
Keyword(s):  
Ethidium Bromide ◽  
Tritiated Thymidine ◽  
Small Degree ◽  
Hemopoietic Cells ◽  
Cellular Kinetics ◽  
Staining Techniques ◽  
Flow Through ◽  
Cellular Dna ◽  
Dna Histograms ◽  
Dna Histogram

The proliferative activity of human neoplasms may be an important determinant for therapeutic management. The advent of automated flow- through systems measuring cellular DNA content by means of fluorescence has considerably facilitated the analysis of cellular kinetics. Using a pulse cytophotometer ICP-11 (Phywe Co., Gottingen, Germany), three different fluorescent staining techniques for DNA histogram measurement on human hemopoietic cells were tested: mithramycin, ethidium bromide, and a combination of ethidium bromide and mithramycin. Employing the tritiated thymidine labeling index as reference standard for comparison with the DNA histogram-derived S-phase fractions, linear correlations were obtained using ethidium bromide alone and ethidium bromide in combination with mithramycin as staining techniques. The fluorescence intensity was increased fourfold to fivefold by the use of the two-dye combination, resulting in a substantial decrease in the coefficient of variation of DNA histograms to 1.5%-2%. This augmented histogram resolution is an important codition for detecting small-degree numeric chromosomal aberrations and discrete drug perturbation effects.

scholarly journals DNA histogram analysis of human hemopoietic cells

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 245-258 ◽  
Author(s):  
B Barlogie ◽  
G Spitzer ◽  
JS Hart ◽  
DA Johnston ◽  
T Buchner ◽  
...  
Keyword(s):  
Ethidium Bromide ◽  
Tritiated Thymidine ◽  
Small Degree ◽  
Hemopoietic Cells ◽  
Cellular Kinetics ◽  
Staining Techniques ◽  
Flow Through ◽  
Cellular Dna ◽  
Dna Histograms ◽  
Dna Histogram

Abstract The proliferative activity of human neoplasms may be an important determinant for therapeutic management. The advent of automated flow- through systems measuring cellular DNA content by means of fluorescence has considerably facilitated the analysis of cellular kinetics. Using a pulse cytophotometer ICP-11 (Phywe Co., Gottingen, Germany), three different fluorescent staining techniques for DNA histogram measurement on human hemopoietic cells were tested: mithramycin, ethidium bromide, and a combination of ethidium bromide and mithramycin. Employing the tritiated thymidine labeling index as reference standard for comparison with the DNA histogram-derived S-phase fractions, linear correlations were obtained using ethidium bromide alone and ethidium bromide in combination with mithramycin as staining techniques. The fluorescence intensity was increased fourfold to fivefold by the use of the two-dye combination, resulting in a substantial decrease in the coefficient of variation of DNA histograms to 1.5%-2%. This augmented histogram resolution is an important codition for detecting small-degree numeric chromosomal aberrations and discrete drug perturbation effects.

Spirals of Mutuality: Love, Nonviolence and Service

FORUM ◽  
2009 ◽  
pp. 31-44
Author(s):  
Aggarwal Suman Khanna
Keyword(s):  
Conflict Resolution ◽  
Sao Paulo ◽  
Plenary Lecture ◽  
Human Interaction ◽  
Single Individual ◽  
Common Goal ◽  
The Common ◽  
Per Se ◽  
Points Of View ◽  
The Law

- In this shortened version of her plenary lecture given at the IAGP Conference in Sao Paulo in July 2006, Suman Khanna Aggarwal reflects on the idea that the welfare of every single individual or group is indissolubly intertwined with that of others and that peace is the common goal of all people and all groups. Since we are all individuals who have differing points of view, conflict often occurs at all levels of human interaction though conflict per se is not a problem; the problem is the method of conflict resolution which can be either violent (bad) or nonviolent (good). It is thus important to understand why we must choose nonviolence to resolve conflict. Gandhi maintains we must choose it because, ‘The Law of Nonviolence which is The Law of Love is the Law of Our Species'. This lecture analyses what constitutes love and transfers this analysis to nonviolence. Once we see how they are related we can start connecting effectively with others.

From Manufacture to Mindfacture

2015 ◽  
pp. 55-86
Keyword(s):  
Common Sense ◽  
Relationship Building ◽  
Human Interaction ◽  
The World ◽  
Manufacturing Company ◽  
The Common

In this chapter, the authors observe the historical shift from man-made to mind-made. They show how the common sense of inherited legacies shaped the attempts to account for how value is added in a product-oriented view that pushes products to market. They show how legacy thinking creates havoc when the focus shifts from things to concerns and accounting practices lose touch with reality. As the world becomes more complex, the need to view the world in a holistic way is assumed. The authors show how a U.S. manufacturing company deals with its own legacies and those created by government practices and legislation. As the world accelerates, human interaction requires increased and more rapid interaction, through relationship building and ever-expanding networks.

Artificial Intelligence in Mental Health

2022 ◽  
pp. 296-319
Author(s):  
Lisa Ogilvie ◽  
Julie Prescott ◽  
Terry Hanley ◽  
Jerome Carson
Keyword(s):  
Mental Health ◽  
Language Processing ◽  
Human Interaction ◽  
Ethical Framework ◽  
Conversational Agents ◽  
Addiction Recovery ◽  
Resultant Solution ◽  
Common Risk Factors ◽  
The Common ◽  
Technological Improvements

Chatbots are programmed conversational agents that emulate communication systematically using natural language processing. They can be programmed to assume a range of roles where regular human interaction occurs. Within mental health services, they are not as well represented as in other areas of healthcare, with research suggesting that uptake has been hindered by concerns over the accuracy of the information they provide, undeveloped technology, lack of adherence to an ethical framework, and the unconvincing portrayal of human authenticity. Technological improvements have addressed some of these concerns, and as the resultant solution choice increases, the potential for chatbots within mental health is receiving greater attention. In this chapter, two novel uses for chatbots are showcased. Foxbot, a recovery friend, accessible at the point of need to help mitigate some of the common risk factors to sustaining addiction recovery; and ERIC, a counselling client who allows trainee counsellors to practise their counselling skills without having to enlist an actual client.

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