scholarly journals ImmunoglobulinλGene Rearrangement Can PrecedeκGene Rearrangement

1990 ◽  
Vol 1 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Jörg Berg ◽  
Mindy Mcdowell ◽  
Hans-Martin Jäck ◽  
Matthias Wabl
Keyword(s):  
B Cell ◽  
Heavy Chain ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Germ Line ◽  
Stochastic Theory ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Line Configuration ◽  
Chain Gene Rearrangement

Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two lightchain loci:κandλ.It has been reported thatκloci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearrangedλ-chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at theκlocus, and that only upon failure to produce a functionalκchain is there an attempt to rearrange theλlocus; and (b) the stochastic theory, which postulates that rearrangement at theλlocus proceeds at a rate that is intrinsically much slower than that at theκlocus. We show here thatλ-chain genes are generated whether or not theκlocus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.

scholarly journals Bruton’s Tyrosine Kinase and SLP-65 Regulate Pre-B Cell Differentiation and the Induction of Ig Light Chain Gene Rearrangement

2006 ◽  
Vol 176 (8) ◽  
pp. 4543-4552 ◽  
Author(s):  
Rogier Kersseboom ◽  
Van B. T. Ta ◽  
A. J. Esther Zijlstra ◽  
Sabine Middendorp ◽  
Hassan Jumaa ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Tyrosine Kinase ◽  
B Cell ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Chain Gene ◽  
B Cell Differentiation ◽  
Bruton's Tyrosine Kinase ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement

scholarly journals Contribution of immunoglobulin lambda light chain gene rearrangement analysis in the diagnosis of B‐cell neoplasms

2019 ◽  
Vol 185 (2) ◽  
pp. 261-265
Author(s):  
Anna L. Paterson ◽  
Hesham El‐Daly ◽  
Livia Raso‐Barnett ◽  
Ming‐Qing Du ◽  
Olivier Giger ◽  
...  
Keyword(s):  
B Cell ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Chain Gene ◽  
Lambda Light Chain ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement ◽  
Gene Rearrangement Analysis ◽  
Immunoglobulin Lambda

scholarly journals Immunoglobulin κ Light Chain Gene Rearrangement Is Impaired in Mice Deficient for DNA Polymerase Mu

Immunity ◽  
2003 ◽  
Vol 19 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Barbara Bertocci ◽  
Annie De Smet ◽  
Claudia Berek ◽  
Jean-Claude Weill ◽  
Claude-Agnès Reynaud
Keyword(s):  
Dna Polymerase ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement

scholarly journals Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 391-397 ◽  
Author(s):  
KA Siminovitch ◽  
JP Jensen ◽  
AL Epstein ◽  
SJ Korsmeyer
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Heavy Chain ◽  
Light Chain ◽  
Cell Lineage ◽  
Chromosomal Translocation ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
Immunoglobulin Gene ◽  
Light Chain Gene

Abstract We have examined the immunoglobulin gene configurations in cell lines from eight patients with diffuse histiocytic lymphoma in order to establish the cellular lineage and stage of differentiation of these lymphomas. The presence of heavy and light chain gene rearrangements as well as heavy chain class switching in seven cells placed these tumors within the B cell lineage. In contrast, one cell (SU-DHL-1), which lacks B cell-restricted surface antigens, retained germline heavy and light chain loci, indicating that it may represent a true histiocyte or uncommitted cell. Truncated RNAs for both the heavy and light chain immunoglobulins were responsible for the lack of surface immunoglobulin in the SU-DHL-2 cell line. Another cell line (SU-DHL-6), which possesses a t(14;18)(q32;q21) translocation, demonstrated an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint.

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