Gene expression profile in mouse myocardium after ischemia

2000 ◽  
Vol 2 (3) ◽  
pp. 93-100 ◽  
Author(s):  
DEBORAH LYN ◽  
XIAOWEI LIU ◽  
NICOLE A. BENNETT ◽  
NERIMIAH L. EMMETT
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Stress Responses ◽  
Muscle Development ◽  
Cardiac Injury ◽  
Cdna Array ◽  
Polymerase Chain Reaction Analysis ◽  
Molecular Profile ◽  
Mouse Heart

Lyn, Deborah, Xiaowei Liu, Nicole A. Bennett, and Nerimiah L. Emmett. Gene expression profile in mouse myocardium after ischemia. Physiol Genomics 2: 93–100, 2000.—This study was designed to elaborate a molecular profile of expressed genes during ischemic injury to the mouse heart after surgical constriction of the left coronary artery without reperfusion. A mouse cDNA array containing 588 known genes was used to compare gene expression in heart RNA after 24-h ischemia with control tissue. Alterations in gene expression on the array were supported by relative reverse transcription-polymerase chain reaction analysis after timed periods of ischemia. Decreased levels of the cell cycle regulator p18ink4 and the oxidative responsive gene glutathione S-transferase were accompanied by an upregulation of the genes associated with cardiac muscle development, α-myosin heavy chain and fetal myosin alkali light chain. Other stress responses elicited by cardiac injury included an induction of Egr-1 and Egr-3 transcription factors, as well as the apoptotic regulator Bax. Altogether, these findings indicate that expression of genes associated with a fetal transcription program may be involved with the post ischemic remodeling process in heart ventricles.

scholarly journals Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

2007 ◽  
Vol 8 (2) ◽  
pp. 88-97 ◽  
Author(s):  
Qing-chao Jin ◽  
Hai-tao Dong ◽  
You-liang Peng ◽  
Bao-shan Chen ◽  
Jing Shao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Magnaporthe Grisea ◽  
Cdna Array

Expression of angiogenesis-related genes in a group of Iranian cases of breast cancer

2020 ◽  
Vol 17 ◽  
Author(s):  
Seyyed Amir Yasin Ahmadi ◽  
Soheila Sayad ◽  
Farhad Shahsavar ◽  
Reza Nekouian ◽  
Mahshid Panahi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Targeted Therapy ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Quantitative Polymerase Chain Reaction ◽  
Molecular Profile ◽  
Benign Tumors ◽  
Study Gene Expression ◽  
Molecular Etiology

Aims: To design an angiogenesis gene expression profile; to study angiogenesis gene expression profile in breast cancer; to map angiogenesis gene expression profile in individual participants. Background: In molecular etiology of each disease, there are some important molecules involved in the related pathways. From the viewpoint of precision medicine, molecular etiology of a disease is different person by person because genetic variations of the genes involved in these pathways. This point of view intend researchers of drug development to design novel drugs for targeted therapy based on the exact etiology. In the case of angiogenesis, there is a drug profile parallel to the molecular profile. Bevacizumab, sunitinib and aflibercept are the examples of anti-angiogenic drugs. Objectives: A hallmark of solid tumors is sustained angiogenesis. Vascular endothelial growth factors (VEGF), VEGF receptors (VEGFR) and placental growth factor (PlGF) are involved in angiogenesis. We aimed to study gene expression profile of angiogenesis including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGFR-1, VEGFR-2, VEGFR-3 and PlGF in an Iranian group of patients undergoing breast surgery due to breast cancer and breast fibroadenoma. Methods: Tumor tissue samples of a group of patients with invasive ductal carcinoma (IDC) and a group of patients with fibroadenoma (Fib) were used. Gene expression was studied by real-time quantitative polymerase chain reaction (q-PCR) and fold changes (FC) with their 95% confidence intervals (CI) were reported based on calibration with normal breast tissue. Results: All the genes showed significant up regulation in IDC group. The most up regulation was for VEGFR-2 (FC=52.68; 95% CI=17.96-154.47; P<0.001). In Fib group, PlGF showed a significant up regulation (FC=10.41; 95% CI=5.35-20.26; P=0.002). Comparison of IDC group with Fib group showed significant up regulation of VEGFR-1, VEGFR-2 and VEGFR-3 in IDC group (P<0.05). Conclusion: Malignancy of breast tumors is associated with over expression of all the genes of this profile. However, only VEGFRs showed up regulation in comparison to benign tumors. Individualized targeted therapy according to this profile should be studied in future.

scholarly journals Transcriptional profiling of bovine muscle-derived satellite cells during differentiation in vitro by high throughput RNA sequencing

2015 ◽  
Vol 20 (3) ◽  
Author(s):  
Hui Li Tong ◽  
Hong Yan Yin ◽  
Wei Wei Zhang ◽  
Qian Hu ◽  
Shu Feng Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
High Throughput ◽  
Satellite Cells ◽  
Muscle Development ◽  
Future Research ◽  
Pathway Enrichment Analysis

AbstractIn this study, we utilized high throughput RNA sequencing to obtain a comprehensive gene expression profile of muscle-derived satellite cells (MDSCs) upon induction of differentiation. MDSCs were cultured in vitro and RNA was extracted for sequencing prior to differentiation (MDSC-P), and again during the early and late differentiation (MDSC-D1, and MDSC-D3, respectively) stages. Sequence tags were assembled and analyzed by digital gene expression profile to screen for differentially expressed genes, Gene Ontology annotation, and pathway enrichment analysis. Quantitative real-time PCR was used to confirm the results of RNA sequencing. Our results indicate that certain of genes were changed during skeletal muscle cell development, cell cycle progression, and cell metabolism during differentiation of bovine MDSCs. Furthermore, we identified certain genes that could be used as novel candidates for future research of muscle development. Additionally, the sequencing results indicated that lipid metabolism might be the predominant cellular process that occurs during MDSC differentiation.

scholarly journals Comprehensive gene expression profile of the adult human renal cortex: Analysis by cDNA array hybridization

2000 ◽  
Vol 57 (4) ◽  
pp. 1452-1459 ◽  
Author(s):  
Naohiro Yano ◽  
Masayuki Endoh ◽  
Kimberly Fadden ◽  
Hiroshi Yamashita ◽  
Agnes Kane ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Renal Cortex ◽  
Cdna Array ◽  
Adult Human ◽  
Array Hybridization

Gene Expression Profile in Human Late Radiation Enteritis Obtained by High-Density cDNA Array Hybridization

2004 ◽  
Vol 161 (3) ◽  
pp. 299-311 ◽  
Author(s):  
Marie-Catherine Vozenin-Brotons ◽  
Fabien Milliat ◽  
Christine Linard ◽  
Carine Strup ◽  
Agnès François ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cdna Array ◽  
High Density ◽  
Radiation Enteritis ◽  
Array Hybridization
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