Hypoxia, Hormones, and Red Blood Cell Function in Chick Embryos

Physiology ◽  
2003 ◽  
Vol 18 (2) ◽  
pp. 77-82 ◽  
Author(s):  
Stefanie Dragon ◽  
Rosemarie Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Adrenergic Receptor ◽  
Cell Function ◽  
Oxygen Affinity ◽  
Terminal Differentiation ◽  
Receptor Activation ◽  
Avian Embryos ◽  
Hypoxic Conditions ◽  
Hemoglobin Oxygen Affinity

The red blood cell function of avian embryos is regulated by cAMP. Adenosine A2A and β-adrenergic receptor activation during hypoxic conditions cause changes in the hemoglobin oxygen affinity and CO2 transport. Furthermore, experimental evidence suggests a general involvement of cAMP in terminal differentiation of avian erythroblasts.

scholarly journals Red Blood Cell Metabolism and Hemoglobin Oxygen Affinity. Effect of Vinburnine on Normobaric Hypoxic Rats.

1999 ◽  
Vol 22 (8) ◽  
pp. 773-774 ◽  
Author(s):  
Adnane LOUAJRI ◽  
Said HARRAGA ◽  
Gerard TOUBIN ◽  
Jean Pierre KANTELIP
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Metabolism ◽  
Oxygen Affinity ◽  
Hemoglobin Oxygen Affinity

Features of ozone effect on oxygen-dependent processes in the blood under hypoxic conditions

2021 ◽  
Vol 20 (3) ◽  
pp. 70-76
Author(s):  
V. V. Zinchuk ◽  
E. S. Biletskaya
Keyword(s):  
Malonic Dialdehyde ◽  
Oxygen Affinity ◽  
Diene Conjugates ◽  
Physiological Factor ◽  
Sodium Hydrosulfide ◽  
Hypoxic Conditions ◽  
Hemoglobin Oxygen Affinity ◽  
Gaseous Transmitters ◽  
Dependent Processes

Introduction. Ozone is a physiological factor that can change hemoglobin oxygen affinity and the formation of gaseous transmitters (NO, H2S). The aim is to study the effect of ozone with gaseous transmitters donors on oxygen-dependent processes in the blood under hypoxic conditions in vitro. Materials and methods. Blood samples were divided into 6 groups of 3 ml each. Groups 2, 4, 5, 6 were pretreated with a deoxygenating gas mixture (5.5 % CO2; 94.5 % N2). In groups 3, 4, 5, 6, ozonized isotonic sodium chloride solution (with an ozone concentration of 6 mg/l) was added, and in groups 5 and 6, the donors of gas transmitters nitroglycerin and sodium hydrosulfide, respectively, were additionally introduced. Results. Pre-deoxygenation reduces the effect of ozone on oxygen transport in the blood. Nitroglycerin prevents this effect. The action of ozone under hypoxic conditions leads to an increase of content of NO3-/NO2- and H2S, and combination with nitroglycerin and sodium hydrosulfide increase these parameters. Deoxygenation due to ozone reduces parameters of lipid peroxidation (malonic dialdehyde, diene conjugates), retinol and α-tocopherol, and the same result in the nitroglycerin group. Conclusion. Under hypoxic conditions, a decrease in the effect of ozone on oxygen-dependent processes is reported. Nitroglycerin reduces its manifestation, while sodium hydrosulfide does not have a similar effect.

scholarly journals Nitric oxide-mediated vasodilation becomes independent of β-adrenergic receptor activation with increased intensity of hypoxic exercise

2011 ◽  
Vol 110 (3) ◽  
pp. 687-694 ◽  
Author(s):  
Darren P. Casey ◽  
Timothy B. Curry ◽  
Brad W. Wilkins ◽  
Michael J. Joyner
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Brachial Artery ◽  
Exercise Intensity ◽  
Adrenergic Receptor ◽  
Receptor Activation ◽  
Adrenergic Mechanisms ◽  
Hypoxic Conditions ◽  
Forearm Exercise ◽  
Hypoxic Exercise

Hypoxic vasodilation in skeletal muscle at rest is known to include β-adrenergic receptor-stimulated nitric oxide (NO) release. We previously reported that the augmented skeletal muscle vasodilation during mild hypoxic forearm exercise includes β-adrenergic mechanisms. However, it is unclear whether a β-adrenergic receptor-stimulated NO component exists during hypoxic exercise. We hypothesized that NO-mediated vasodilation becomes independent of β-adrenergic receptor activation with increased exercise intensity during hypoxic exercise. Ten subjects (7 men, 3 women; 23 ± 1 yr) breathed hypoxic gas to titrate arterial O2 saturation to 80% while remaining normocapnic. Subjects performed two consecutive bouts of incremental rhythmic forearm exercise (10% and 20% of maximum) with local administration (via a brachial artery catheter) of propranolol (β-adrenergic receptor inhibition) alone and with the combination of propranolol and nitric oxide synthase inhibition [ NG-monomethyl-l-arginine (l-NMMA)] under normoxic and hypoxic conditions. Forearm blood flow (FBF, ml/min; Doppler ultrasound) and blood pressure [mean arterial pressure (MAP), mmHg; brachial artery catheter] were assessed, and forearm vascular conductance (FVC, ml·min−1·100 mmHg−1) was calculated (FBF/MAP). During propranolol alone, the rise in FVC (Δ from normoxic baseline) due to hypoxic exercise was 217 ± 29 and 415 ± 41 ml·min−1·100 mmHg−1 (10% and 20% of maximum, respectively). Combined propranolol-l-NMMA infusion during hypoxic exercise attenuated ΔFVC at 20% (352 ± 44 ml·min−1·100 mmHg−1; P < 0.001) but not at 10% (202 ± 28 ml·min−1·100 mmHg−1; P = 0.08) of maximum compared with propranolol alone. These data, when integrated with earlier findings, demonstrate that NO contributes to the compensatory vasodilation during mild and moderate hypoxic exercise; a β-adrenergic receptor-stimulated NO component exists during low-intensity hypoxic exercise. However, the source of the NO becomes less dependent on β-adrenergic mechanisms as exercise intensity increases.

scholarly journals SILAC Mouse for Quantitative Proteomics Uncovers Kindlin-3 as an Essential Factor for Red Blood Cell Function

Cell ◽  
2008 ◽  
Vol 134 (2) ◽  
pp. 353-364 ◽  
Author(s):  
Marcus Krüger ◽  
Markus Moser ◽  
Siegfried Ussar ◽  
Ingo Thievessen ◽  
Christian A. Luber ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Quantitative Proteomics ◽  
Cell Function ◽  
Essential Factor

scholarly journals Red Blood Cell 2,3-Diphosphoglycerate Decreases in Response to a 30 km Time Trial Under Hypoxia in Cyclists

2021 ◽  
Vol 12 ◽  
Author(s):  
Kamila Płoszczyca ◽  
Miłosz Czuba ◽  
Małgorzata Chalimoniuk ◽  
Robert Gajda ◽  
Marcin Baranowski
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Average Power ◽  
Time Trial ◽  
Intense Exercise ◽  
Acid Base ◽  
Hypoxic Conditions ◽  
Acid Base Equilibrium ◽  
Dissociation Curves ◽  
2,3 Dpg

Red blood cell 2,3-diphosphoglycerate (2,3-DPG) is one of the factors of rightward-shifted oxygen dissociation curves and decrease of Hb-O2 affinity. The reduction of Hb-O2 affinity is beneficial to O2 unloading at the tissue level. In the current literature, there are no studies about the changes in 2,3-DPG level following acute exercise in moderate hypoxia in athletes. For this reason, the aim of this study was to analyze the effect of prolonged intense exercise under normoxic and hypoxic conditions on 2,3-DPG level in cyclists. Fourteen male trained cyclists performed a simulation of a 30 km time trial (TT) in normoxia and normobaric hypoxia (FiO2 = 16.5%, ~2,000 m). During the TT, the following variables were measured: power, blood oxygen saturation (SpO2), and heart rate (HR). Before and immediately after exercise, the blood level of 2,3-DPG and acid–base equilibrium were determined. The results showed that the mean SpO2 during TT in hypoxia was 8% lower than in normoxia. The reduction of SpO2 in hypoxia resulted in a decrease of average power by 9.6% (p &lt; 0.001) and an increase in the 30 km TT completion time by 3.8% (p &lt; 0.01) compared to normoxia. The exercise in hypoxia caused a significant (p &lt; 0.001) decrease in 2,3-DPG level by 17.6%. After exercise in normoxia, a downward trend of 2,3-DPG level was also observed, but this effect was not statistically significant. The analysis also revealed that changes of acid–base balance were significantly larger (p &lt; 0.05) after exercise in hypoxia than in normoxia. In conclusion, intense exercise in hypoxic conditions leads to a decrease in 2,3-DPG concentration, primarily due to exercise-induced acidosis.

Biochemical bases for difference in oxygen affinity of maternal and fetal red blood cells of rattlesnakes

1993 ◽  
Vol 264 (3) ◽  
pp. R481-R486
Author(s):  
F. R. Ragsdale ◽  
R. L. Ingermann
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Oxygen Affinity ◽  
Fetal Hemoglobin ◽  
Nucleoside Triphosphate ◽  
Biochemical Basis ◽  
Crotalus Viridis ◽  
Adult Hemoglobin

Pregnancy in Crotalus viridis oreganus is associated with an increase in the nucleoside triphosphate (NTP) concentration and a concomitant decrease in the oxygen affinity of the adult red blood cell. However, although the red blood cells of non-pregnant adults and fetuses have indistinguishable NTP concentrations, they have different oxygen affinities. Therefore, red blood cell NTP concentrations alone cannot account for the oxygen-affinity difference between fetal and maternal red blood cells. Hemoglobins from adult and fetal snakes had similar intrinsic oxygen affinities; however, adult hemoglobin was more responsive to organic phosphate modulation compared with fetal hemoglobin. Structural differences, indicated by native gel electrophoresis and electrophoresis of the globins under denaturing conditions at high pH, corroborated functional differences of hemoglobins from fetus and adult. Therefore, the biochemical basis for the oxygen-affinity difference between maternal and fetal red blood cells in this rattlesnake appears to be unique. It appears to be caused by a functionally distinct fetal hemoglobin and the pregnancy-associated rise in red blood cell NTP levels in the mother.

Sign in / Sign up

Export Citation Format

Share Document