scholarly journals Imaging light responses of retinal ganglion cells in the living mouse eye

2013 ◽  
Vol 109 (9) ◽  
pp. 2415-2421 ◽  
Author(s):  
Lu Yin ◽  
Ying Geng ◽  
Fumitaka Osakada ◽  
Robin Sharma ◽  
Ali H. Cetin ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Morphological Development ◽  
Retinal Ganglion ◽  
Calcium Indicator ◽  
In Vitro Physiology

This study reports development of a novel method for high-resolution in vivo imaging of the function of individual mouse retinal ganglion cells (RGCs) that overcomes many limitations of available methods for recording RGC physiology. The technique combines insertion of a genetically encoded calcium indicator into RGCs with imaging of calcium responses over many days with FACILE (functional adaptive optics cellular imaging in the living eye). FACILE extends the most common method for RGC physiology, in vitro physiology, by allowing repeated imaging of the function of each cell over many sessions and by avoiding damage to the retina during removal from the eye. This makes it possible to track changes in the response of individual cells during morphological development or degeneration. FACILE also overcomes limitations of existing in vivo imaging methods, providing fine spatial and temporal detail, structure-function comparison, and simultaneous analysis of multiple cells.

Tafluprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo

2009 ◽  
Vol 247 (10) ◽  
pp. 1353-1360 ◽  
Author(s):  
Akiyasu Kanamori ◽  
Maiko Naka ◽  
Masahide Fukuda ◽  
Makoto Nakamura ◽  
Akira Negi
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

miR-223 induces retinal ganglion cells apoptosis and inflammation via decreasing HSP-70 in vitro and in vivo

2020 ◽  
Vol 104 ◽  
pp. 101747 ◽  
Author(s):  
Yun Ou-Yang ◽  
Zheng-Li Liu ◽  
Chun-Long Xu ◽  
Jia-Liang Wu ◽  
Jun Peng ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Hsp 70 ◽  
Apoptosis And Inflammation

scholarly journals Brazilian Green Propolis Protects against Retinal Damage In Vitro and In Vivo

2006 ◽  
Vol 3 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Yuta Inokuchi ◽  
Masamitsu Shimazawa ◽  
Yoshimi Nakajima ◽  
Shinsuke Suemori ◽  
Satoshi Mishima ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Water Soluble ◽  
Retinal Damage ◽  
Retinal Ganglion ◽  
Neuroprotective Effects ◽  
Brazilian Green Propolis

Propolis, a honeybee product, has gained popularity as a food and alternative medicine. Its constituents have been shown to exert pharmacological (anticancer, antimicrobial and anti-inflammatory) effects. We investigated whether Brazilian green propolis exerts neuroprotective effects in the retinain vitroand/orin vivo.In vitro, retinal damage was induced by 24 h hydrogen peroxide (H2O2) exposure, and cell viability was measured by Hoechst 33342 and YO-PRO-1 staining or by a resazurin–reduction assay. Propolis inhibited the neurotoxicity and apoptosis induced in cultured retinal ganglion cells (RGC-5, a rat ganglion cell line transformed using E1A virus) by 24 h H2O2 exposure. Propolis also inhibited the neurotoxicity induced in RGC-5 cultures by staurosporine. Regarding the possible underlying mechanism, in pig retina homogenates propolis protected against oxidative stress (lipid peroxidation), as also did trolox (water-soluble vitamin E). In micein vivo, propolis (100 mg kg−1; intraperitoneally administered four times) reduced the retinal damage (decrease in retinal ganglion cells and in thickness of inner plexiform layer) induced by intravitrealin vivo N-methyl-d-aspartate injection. These findings indicate that Brazilian green propolis has neuroprotective effects against retinal damage bothin vitroandin vivo, and that a propolis-induced inhibition of oxidative stress may be partly responsible for these neuroprotective effects.

In Vivo Imaging of the Fine Structure of Rhodamine-Labeled Macaque Retinal Ganglion Cells

2008 ◽  
Vol 49 (1) ◽  
pp. 467 ◽  
Author(s):  
Daniel C. Gray ◽  
Robert Wolfe ◽  
Bernard P. Gee ◽  
Drew Scoles ◽  
Ying Geng ◽  
...  
Keyword(s):  
Fine Structure ◽  
Retinal Ganglion Cells ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Retinal Ganglion

Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo

2009 ◽  
Vol 88 (3) ◽  
pp. 535-541 ◽  
Author(s):  
Akiyasu Kanamori ◽  
Maiko Naka ◽  
Masahide Fukuda ◽  
Makoto Nakamura ◽  
Akira Negi
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Long-Term In Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells

2011 ◽  
Vol 52 (3) ◽  
pp. 1539 ◽  
Author(s):  
Christopher Kai-shun Leung ◽  
Robert N. Weinreb ◽  
Zhi Wei Li ◽  
Shu Liu ◽  
James D. Lindsey ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Retinal Ganglion

Optical properties of retinal tissue and the potential of adaptive optics to visualize retinal ganglion cells in vivo

2013 ◽  
Vol 353 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Martina Prasse ◽  
Franziska Georgia Rauscher ◽  
Peter Wiedemann ◽  
Andreas Reichenbach ◽  
Mike Francke
Keyword(s):  
Optical Properties ◽  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Retinal Tissue

scholarly journals Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Bikun Xian ◽  
Ziming Luo ◽  
Kaijing Li ◽  
Kang Li ◽  
Mingjun Tang ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Immunosuppressive Treatment ◽  
Retinal Ganglion ◽  
Chain Reaction ◽  
Immunological Rejection ◽  
Polymerase Chain

We investigated the efficacy of the immunosuppressants rapamycin (RAP) and dexamethasone (DEX) in improving the survival of retinal organoids after epiretinal transplantation. We first compared the immunosuppressive abilities of DEX and RAP in activated microglia in an in vitro setting. Following this, we used immunofluorescence, real-time polymerase chain reaction, and flow cytometry to investigate the effects of DEX and RAP on cells in the retinal organoids. Retinal organoids were then seeded onto poly(lactic-co-glycolic) acid (PLGA) scaffolds and implanted into rhesus monkey eyes (including a healthy individual and three monkeys with chronic ocular hypertension (OHT) induction) and subjected to different post-operative immunosuppressant treatments; 8 weeks after the experiment, histological examinations were carried out to assess the success of the different treatments. Our in vitro experiments indicated that both DEX and RAP treatments were equally effective in suppressing microglial activity. Although both immunosuppressants altered the morphologies of cells in the retinal organoids and caused a slight decrease in the differentiation of cells into retinal ganglion cells, the organoid cells retained their capacity to grow and differentiate into retinal tissues. Our in vivo experiments indicate that the retinal organoid can survive and differentiate into retinal tissues in a healthy rhesus monkey eye without immunosuppressive treatment. However, the survival and differentiation of these organoids in OHT eyes was successful only with the DEX treatment. RAP treatment was ineffective in preventing immunological rejection, and the retinal organoid failed to survive until the end of 8 weeks. DEX is likely a promising immunosuppressant to enhance the survival of epiretinal implants.

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