Enhancing contrast of in-vivo human retinal ganglion cells with multi-offset adaptive optics scanning laser ophthalmoscope

2021 ◽  
Author(s):  
Elena Gofas-Salas ◽  
Yuhua Rui ◽  
Pedro Mece ◽  
Min Zhang ◽  
Valerie C. Snyder ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
Ganglion Cells ◽  
Scanning Laser Ophthalmoscope ◽  
Scanning Laser ◽  
Retinal Ganglion

In Vivo Imaging and Counting of Rat Retinal Ganglion Cells Using a Scanning Laser Ophthalmoscope

2006 ◽  
Vol 47 (7) ◽  
pp. 2943 ◽  
Author(s):  
Tomomi Higashide ◽  
Ichiro Kawaguchi ◽  
Shinji Ohkubo ◽  
Hisashi Takeda ◽  
Kazuhisa Sugiyama
Keyword(s):  
Retinal Ganglion Cells ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Scanning Laser Ophthalmoscope ◽  
Scanning Laser ◽  
Retinal Ganglion

Optical properties of retinal tissue and the potential of adaptive optics to visualize retinal ganglion cells in vivo

2013 ◽  
Vol 353 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Martina Prasse ◽  
Franziska Georgia Rauscher ◽  
Peter Wiedemann ◽  
Andreas Reichenbach ◽  
Mike Francke
Keyword(s):  
Optical Properties ◽  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Retinal Tissue

scholarly journals Spatial frequency-based image reconstruction to improve image contrast in multi-offset adaptive optics ophthalmoscopy

2020 ◽  
Author(s):  
Pedro Mecê ◽  
Elena Gofas ◽  
Yuhua Rui ◽  
Min Zhang ◽  
José-Alain Sahel ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Spatial Frequency ◽  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
Phase Contrast ◽  
Ganglion Cells ◽  
Image Contrast ◽  
Detection Methods ◽  
Retinal Ganglion

Off-axis detection methods in adaptive optics (AO) ophthalmoscopy can enhance image contrast of translucent retinal structures such as cone inner segments and retinal ganglion cells layer neurons. Here, we propose a 2D optical model showing that the phase contrast produced by these methods depends on the offset orientation. While one axis provides an asymmetric light distribution, hence a high phase contrast, the perpendicular axis provides a symmetric one, thus a substantially lower contrast. We support this model with in-vivo human data acquired with a multi-offset AO scanning light ophthalmoscope. Then, using this finding, we provide a post-processing method, named Spatial frequency-based iMAge ReconsTruction (SMART), to optimally combine images from different off-axis detector orientations, significantly increasing the structural cellular contrast of in-vivo human retinal neurons such as conne inner segment, putative rods and retinal ganglion cells.

scholarly journals Imaging light responses of retinal ganglion cells in the living mouse eye

2013 ◽  
Vol 109 (9) ◽  
pp. 2415-2421 ◽  
Author(s):  
Lu Yin ◽  
Ying Geng ◽  
Fumitaka Osakada ◽  
Robin Sharma ◽  
Ali H. Cetin ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Morphological Development ◽  
Retinal Ganglion ◽  
Calcium Indicator ◽  
In Vitro Physiology

This study reports development of a novel method for high-resolution in vivo imaging of the function of individual mouse retinal ganglion cells (RGCs) that overcomes many limitations of available methods for recording RGC physiology. The technique combines insertion of a genetically encoded calcium indicator into RGCs with imaging of calcium responses over many days with FACILE (functional adaptive optics cellular imaging in the living eye). FACILE extends the most common method for RGC physiology, in vitro physiology, by allowing repeated imaging of the function of each cell over many sessions and by avoiding damage to the retina during removal from the eye. This makes it possible to track changes in the response of individual cells during morphological development or degeneration. FACILE also overcomes limitations of existing in vivo imaging methods, providing fine spatial and temporal detail, structure-function comparison, and simultaneous analysis of multiple cells.

scholarly journals Spatial receptive field properties of rat retinal ganglion cells

2011 ◽  
Vol 28 (5) ◽  
pp. 403-417 ◽  
Author(s):  
WALTER F. HEINE ◽  
CHRISTOPHER L. PASSAGLIA
Keyword(s):  
Receptive Field ◽  
Ganglion Cell ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Cell Types ◽  
Quantitative Information ◽  
Retinal Ganglion ◽  
X And Y Cells ◽  
Y Cells

AbstractThe rat is a popular animal model for vision research, yet there is little quantitative information about the physiological properties of the cells that provide its brain with visual input, the retinal ganglion cells. It is not clear whether rats even possess the full complement of ganglion cell types found in other mammals. Since such information is important for evaluating rodent models of visual disease and elucidating the function of homologous and heterologous cells in different animals, we recorded from rat ganglion cells in vivo and systematically measured their spatial receptive field (RF) properties using spot, annulus, and grating patterns. Most of the recorded cells bore likeness to cat X and Y cells, exhibiting brisk responses, center-surround RFs, and linear or nonlinear spatial summation. The others resembled various types of mammalian W cell, including local-edge-detector cells, suppressed-by-contrast cells, and an unusual type with an ON–OFF surround. They generally exhibited sluggish responses, larger RFs, and lower responsiveness. The peak responsivity of brisk-nonlinear (Y-type) cells was around twice that of brisk-linear (X-type) cells and several fold that of sluggish cells. The RF size of brisk-linear and brisk-nonlinear cells was indistinguishable, with average center and surround diameters of 5.6 ± 1.3 and 26.4 ± 11.3 deg, respectively. In contrast, the center diameter of recorded sluggish cells averaged 12.8 ± 7.9 deg. The homogeneous RF size of rat brisk cells is unlike that of cat X and Y cells, and its implication regarding the putative roles of these two ganglion cell types in visual signaling is discussed.

scholarly journals Otx2 Promotes the Survival of Damaged Adult Retinal Ganglion Cells and Protects against Excitotoxic Loss of Visual Acuity In Vivo

2011 ◽  
Vol 31 (14) ◽  
pp. 5495-5503 ◽  
Author(s):  
R. T. Ibad ◽  
J. Rheey ◽  
S. Mrejen ◽  
V. Forster ◽  
S. Picaud ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Loss Of Visual Acuity
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