Long-term effects of diet on leptin, energy intake, and activity in a model of diet-induced obesity

2002 ◽  
Vol 93 (3) ◽  
pp. 887-893 ◽  
Author(s):  
Christian K. Roberts ◽  
Joshua J. Berger ◽  
R. James Barnard
Keyword(s):  
Body Temperature ◽  
Cell Size ◽  
Energy Intake ◽  
Feed Efficiency ◽  
Dietary Intervention ◽  
Leptin Resistance ◽  
Activity Levels ◽  
Adipocyte Size ◽  
Diet Induced Obesity

This study investigated the effect of long-term high-fat sucrose (HFS) or low-fat complex-carbohydrate (LFCC) diet consumption on leptin, insulin, fat cell size, energy intake, and markers of activity to ascertain the role that leptin plays in long-term energy balance in a model of diet-induced obesity. Female Fischer 344 rats were fed either a HFS or LFCC diet ad libitum for a period of 20 mo. Measurements of leptin concentration, insulin concentration, and adipocyte size were performed at 2 wk, 2 mo, 6 mo, and 20 mo. Body weight and energy intake were measured weekly for calculation of feed efficiency. Body temperature and activity levels were assessed over a 5-day period after 12 mo of the dietary intervention. Plasma leptin and insulin concentrations were significantly elevated within 2 wk of HFS diet consumption and remained elevated throughout the course of the study. After 2 mo, the adipocytes of the HFS group were significantly larger and continued to increase in size throughout the course of the study. A significant correlation was noted between leptin and adipocyte cell size ( r = 0.96, P < 0.01). However, despite elevated leptin, energy intake was similar, and the HFS group weighed significantly more than the LFCC group, as a result of a higher feed efficiency. There were no significant differences in body temperature or activity levels between the groups. These results demonstrate that a HFS diet causes hyperleptinemia and hyperinsulinemia before adipocyte size is increased and suggests that leptin resistance may be present or, alternatively, that leptin does not to play a major role in the long-term regulation of energy intake or activity levels in this model.

Energy Needs of Athletes

2001 ◽  
Vol 26 (S1) ◽  
pp. S202-S219 ◽  
Author(s):  
Louise M. Burke
Keyword(s):  
Energy Intake ◽  
Energy Requirements ◽  
Activity Levels ◽  
Record Keeping ◽  
Energy Needs ◽  
Self Reports ◽  
Optimal Health ◽  
And Performance ◽  
True Energy

Each athlete has unique energy requirements, which underpin their ability to meet total nutritional goals. For everyday dietary planning and evaluation, energy requirements can be predicted via estimations of RMR and activity levels. Research methods such as indirect calorimetry and DLW allow energy requirements to be measured, and may be useful to confirm situations in which an athlete has a true energy balance anomaly. There is some evidence that individual athletes may have reduced energy requirements, although this occurs less frequently than is reported. Most self-reports of food intake substantially under-estimate energy intake, due to under-reporting or under-eating during the period of record keeping. Many athletes are over-focused on reducing body mass and body fat below levels that are consistent with long-term health and performance. Restrained eating can cause significant detrimental outcomes to body function. Leptin may be involved in modulating or mediating some of these changes. Athletes should use their energy budget to choose foods that provide macronutrient and micronutrient needs for optimal health and performance. Practical advice may help athletes to achieve energy intake challenges.

scholarly journals Long-Term Bed Rest Delays the Circadian Phase of Core Body Temperature

2021 ◽  
Vol 12 ◽  
Author(s):  
Stefan Mendt ◽  
Katharina Brauns ◽  
Anika Friedl-Werner ◽  
Daniel L. Belavy ◽  
Mathias Steinach ◽  
...  
Keyword(s):  
Physical Activity ◽  
Body Temperature ◽  
Circadian Rhythms ◽  
Core Body Temperature ◽  
Bed Rest ◽  
Phase Delay ◽  
Activity Levels ◽  
Physical Activity Levels ◽  
Core Body

Spaceflight can be associated with sleep loss and circadian misalignment as a result of non-24 h light-dark cycles, operational shifts in work/rest cycles, high workload under pressure, and psychological factors. Head-down tilt bed rest (HDBR) is an established model to mimic some of the physiological and psychological adaptions observed in spaceflight. Data on the effects of HDBR on circadian rhythms are scarce. To address this gap, we analyzed the change in the circadian rhythm of core body temperature (CBT) in two 60-day HDBR studies sponsored by the European Space Agency [n = 13 men, age: 31.1 ± 8.2 years (M ± SD)]. CBT was recorded for 36 h using a non-invasive and validated dual-sensor heatflux technology during the 3rd and the 8th week of HDBR. Bed rest induced a significant phase delay from the 3rd to the 8th week of HDBR (16.23 vs. 16.68 h, p = 0.005, g = 0.85) irrespective of the study site (p = 0.416, g = −0.46), corresponding to an average phase delay of about 0.9 min per day of HDBR. In conclusion, long-term bed rest weakens the entrainment of the circadian system to the 24-h day. We attribute this effect to the immobilization and reduced physical activity levels associated with HDBR. Given the critical role of diurnal rhythms for various physiological functions and behavior, our findings highlight the importance of monitoring circadian rhythms in circumstances in which gravity or physical activity levels are altered.

scholarly journals The Impact of Isolation Measures Due to COVID-19 on Energy Intake and Physical Activity Levels in Australian University Students

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1865 ◽  
Author(s):  
Linda A. Gallo ◽  
Tania F. Gallo ◽  
Sophia L. Young ◽  
Karen M. Moritz ◽  
Lisa K. Akison
Keyword(s):  
Physical Activity ◽  
Energy Intake ◽  
Activity Levels ◽  
Physical Isolation ◽  
Home Based ◽  
Physical Activity Levels ◽  
Reduced Physical Activity ◽  
The Impact

The coronavirus disease 2019 (COVID-19) pandemic resulted in physical isolation measures in many parts of the world. In Australia, nationwide restrictions included staying at home, unless seeking medical care, providing care, purchasing food, undertaking exercise, or attending work in an essential service. All undergraduate university classes transitioned to online, mostly home-based learning. We, therefore, examined the effect of isolation measures during the early phase of the COVID-19 pandemic in Australia (March/April) on diet (24-h recall) and physical activity (Active Australia Survey) patterns in third-year biomedical students. Findings were compared with students enrolled in the same course in the previous two years. In females, but not males, energy intake was ~20% greater during the pandemic, and snacking frequency and energy density of consumed snacks also increased compared with 2018 and 2019. Physical activity was impacted for both sexes during the pandemic with ~30% fewer students achieving “sufficient” levels of activity, defined by at least 150 min over at least five sessions, compared with the previous two years. In a follow-up study six to eight weeks later (14–18% response rate), during gradual easing of nationwide restrictions albeit continued gym closures and online learning, higher energy intake in females and reduced physical activity levels in both sexes persisted. These data demonstrate the health impacts of isolation measures, with the potential to affect long-term diet and activity behaviours.

Early postnatal leptin blockage leads to a long-term leptin resistance and susceptibility to diet-induced obesity in rats

2008 ◽  
Vol 32 (7) ◽  
pp. 1153-1160 ◽  
Author(s):  
L Attig ◽  
G Solomon ◽  
J Ferezou ◽  
L Abdennebi-Najar ◽  
M Taouis ◽  
...  
Keyword(s):  
Leptin Resistance ◽  
Diet Induced Obesity

scholarly journals Lentiviral vector-mediated knockdown of Lrb in the arcuate nucleus promotes diet-induced obesity in rats

2013 ◽  
Vol 51 (1) ◽  
pp. 27-35 ◽  
Author(s):  
J Bian ◽  
X M Bai ◽  
Y L Zhao ◽  
L Zhang ◽  
Z J Liu
Keyword(s):  
Energy Intake ◽  
Arcuate Nucleus ◽  
Leptin Receptor ◽  
Lentiviral Vector ◽  
Male Rats ◽  
Leptin Resistance ◽  
Chow Diet ◽  
Metabolic Complications ◽  
Diet Induced Obesity ◽  
Obese Human

Obesity is currently a worldwide pandemic. Leptin resistance is a main mechanism of obese human and rodents. The downregulation of the long form of the leptin receptor (Lrb) was involved in leptin resistance in diet-induced obese rats. In the studies, we investigated whether arcuate nucleus (ARC) silencing ofLrbwould promote diet-induced obesity in rats. Lentiviral vectors expressingLrb-shRNA were administered to 5-week-old male rats by ARC injection. Following viral delivery, the rats were provided with a high-fat diet (HFD) or a chow diet (CD). After 8 weeks of the diet, serum leptin, and insulin concentrations were measured by RIA, gene expression ofLrbin the ARC was detected by a real-time RT-PCR, and leptin signaling was examined by western blot. TheLrb-shRNA knocked down the expression ofLrbmRNA in infected regions by 54% for the HFD rats and 47% for the CD rats respectively. TheLrbknockdown reduced Stats3 activation and increased expression ofNpymRNA. The rats with reducedLrbin the ARC showed a significant increase in energy intake and body weight (BW) again when fed with a HFD. By contrast, there were no effects ofLrbreduction on energy intake or BW when rats maintained on a low-fat chow. Our results provide evidence thatLrbknockdown selectively in the ARC promotes diet-induced obesity and associated metabolic complications in rats.

Trans-palmitoleic Acid Reduces Adiposity via Increased Lipolysis in a Rodent Model of Diet-Induced Obesity

2021 ◽  
pp. 1-24
Author(s):  
L. Irasema Chávaro-Ortiz ◽  
Brenda D. Tapia-Vargas ◽  
Mariel Rico-Hidalgo ◽  
Ruth Gutiérrez-Aguilar ◽  
María E. Frigolet
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Visceral Adipose Tissue ◽  
High Fat Diet ◽  
Palmitoleic Acid ◽  
Rodent Model ◽  
Adipocyte Size ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Visceral Adipose

Abstract Obesity is defined as increased adiposity, which leads to metabolic disease. The growth of adipose tissue depends on its capacity to expand, through hyperplasia or hypertrophy, in order to buffer energy surplus. Also, during the establishment of obesity, adipose tissue expansion reflects adipose lipid metabolism (lipogenesis and/or lipolysis). It is well known that dietary factors can modify lipid metabolism promoting or preventing the development of metabolic abnormalities that concur with obesity. Trans-palmitoleic acid (TP), a biomarker of dairy consumption, has been associated with reduced adiposity in clinical studies. Thus, we aimed to evaluate the effect of TP over adiposity and lipid metabolism-related genes in a rodent model of diet-induced obesity (DIO). To fulfil this aim, we fed C57BL/6 mice with a Control or a High Fat diet, added with or without TP (3g/kg diet), during 11 weeks. Body weight and food intake were monitored, fat pads were weighted, histology of visceral adipose tissue was analysed, and lipid metabolism-related gene expression was explored by qPCR. Results show that TP consumption prevented weight gain induced by high fat diet, reduced visceral adipose tissue weight, and adipocyte size, while increasing the expression of lipolytic molecules. In conclusion, we show for the first time that TP influences adipose tissue metabolism, specifically lipolysis, resulting in decreased adiposity and reduced adipocyte size in a DIO mice model.

scholarly journals Effect of a High-Intensity Dietary Intervention on Changes in Dietary Intake and Eating Pathology during a Multicomponent Adolescent Obesity Intervention

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1850
Author(s):  
Hollie A. Raynor ◽  
Suzanne E. Mazzeo ◽  
Jessica Gokee LaRose ◽  
Elizabeth L. Adams ◽  
Laura M. Thornton ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Energy Intake ◽  
Diet Quality ◽  
Dietary Intervention ◽  
Secondary Data ◽  
Obesity Treatment ◽  
Healthy Eating Index ◽  
Eating Pathology ◽  
Post Treatment ◽  
Eating Disorder Examination

Concerns remain about dietary changes during pediatric obesity treatment and eating pathology, which have not been investigated. This secondary data analysis from a randomized clinical trial examined associations between adolescents’ changes in energy intake and diet quality during obesity treatment with post-treatment eating pathology. Adolescents (N = 82: 13.7 ± 1.2 y, 34.9 ± 7.0 kg/m2, 63.4% female, 46.3% black) received TEENS+, a 4-month multicomponent intervention. TEENS+ provided individualized dietary goals (1200–1800 kcal/day; number of “Go” foods/day (low-energy, high-nutrient-dense foods)). At 0 and 4 months, 3-day food records assessed energy intake and diet quality (Healthy Eating Index 2015 (HEI-2015)). Two HEI-2015 subscores were created: components to increase (increase), and components to limit (decrease). The Eating Disorder Examination Questionnaire measured eating pathology (total score and subscales: restraint; and eating, weight, and shape concern). Corrected p-values are reported as q-values. Energy intake decreased (−292 ± 418 kcal/day; q < 0.001), while diet quality improved during treatment (total HEI-2015 (4.5 ± 15.1; q = 0.034) and increase (3.3 ± 9.4; q = 0.011)). Restraint increased (+0.6 ± 1.4; q < 0.001), whereas shape (−0.5 ± 1.3; q = 0.004) and weight (−0.5 ± 1.4; q = 0.015) concerns decreased. Greater decreases in energy intake were associated with greater restraint post-treatment (F = 17.69; q < 0.001). No other significant associations were observed. Changes in adolescents’ dietary intake during obesity treatment were unrelated to increased shape, weight, or eating concerns post-treatment.

scholarly journals Effects of a Protein-Rich, Low-Glycaemic Meal Replacement on Changes in Dietary Intake and Body Weight Following a Weight-Management Intervention—The ACOORH Trial

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 376
Author(s):  
Martin Röhling ◽  
Andrea Stensitzky ◽  
Camila L. P. Oliveira ◽  
Andrea Beck ◽  
Klaus Michael Braumann ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Energy Intake ◽  
Lifestyle Intervention ◽  
Weight Change ◽  
Protein Intake ◽  
Daily Energy Intake ◽  
Meal Replacement ◽  
Daily Energy ◽  
Protein Rich

Although meal replacement can lead to weight reduction, there is uncertainty whether this dietary approach implemented into a lifestyle programme can improve long-term dietary intake. In this subanalysis of the Almased Concept against Overweight and Obesity and Related Health Risk (ACOORH) study (n = 463), participants with metabolic risk factors were randomly assigned to either a meal replacement-based lifestyle intervention group (INT) or a lifestyle intervention control group (CON). This subanalysis relies only on data of participants (n = 119) who returned correctly completed dietary records at baseline, and after 12 and 52 weeks. Both groups were not matched for nutrient composition at baseline. These data were further stratified by sex and also associated with weight change. INT showed a higher increase in protein intake related to the daily energy intake after 12 weeks (+6.37% [4.69; 8.04] vs. +2.48% [0.73; 4.23], p < 0.001) of intervention compared to CON. Fat and carbohydrate intake related to the daily energy intake were more strongly reduced in the INT compared to CON (both p < 0.01). After sex stratification, particularly INT-women increased their total protein intake after 12 (INT: +12.7 g vs. CON: −5.1 g, p = 0.021) and 52 weeks (INT: +5.7 g vs. CON: −16.4 g, p = 0.002) compared to CON. Protein intake was negatively associated with weight change (r = −0.421; p < 0.001) after 12 weeks. The results indicate that a protein-rich dietary strategy with a meal replacement can improve long-term nutritional intake, and was associated with weight loss.

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