T-cells and muscle just don't talk like they used to: Focus on “Age-related impairment of T cell-induced skeletal muscle precursor cell function”

2011 ◽  
Vol 300 (6) ◽  
pp. C1223-C1225 ◽  
Author(s):  
Thomas J. Hawke
Keyword(s):  
Skeletal Muscle ◽  
T Cells ◽  
T Cell ◽  
Cell Function ◽  
Precursor Cell ◽  
Muscle Precursor ◽  
Age Related ◽  
Muscle Precursor Cell

scholarly journals Age-related impairment of T cell-induced skeletal muscle precursor cell function

2011 ◽  
Vol 300 (6) ◽  
pp. C1226-C1233 ◽  
Author(s):  
Breanna R. Dumke ◽  
Simon J. Lees
Keyword(s):  
Skeletal Muscle ◽  
T Cells ◽  
T Cell ◽  
Cell Function ◽  
Critical Role ◽  
Interleukin 2 ◽  
Precursor Cell ◽  
Muscle Precursor ◽  
Age Related ◽  
Muscle Precursor Cell

Sarcopenia is the age-associated loss of skeletal muscle mass and strength. Recent evidence suggests that an age-associated loss of muscle precursor cell (MPC) functionality contributes to sarcopenia. The objectives of the present study were to examine the influence of activated T cells on MPCs and determine whether an age-related defect in this signaling occurs. MPCs were collected from the gastrocnemius and plantaris of 3-mo-old (young) and 32-mo-old (old) animals. Splenic T cells were harvested using anti-CD3 Dynabead isolation. T cells were activated for 48 h with costimulation of 100 IU/ml interleukin-2 (IL-2) and 5 μg/ml of anti-CD28. Costimulation increased 5-bromo-2′-deoxyuridine incorporation of T cells from 13.4 ± 4.6% in control to 64.8 ± 6.0% in costimulated cells. Additionally, T cell cytokines increased proliferation on MPCs isolated from young muscle by 24.0 ± 5.7%, whereas there was no effect on MPCs isolated from aged muscle. T cell cytokines were also found to be a chemoattractant. T cells were able to promote migration of MPCs isolated from young muscle; however, MPCs isolated from aged muscle did not respond to the T cell-released chemokines. Conversely, whereas T cell-released cytokines did not affect myogenesis of MPCs isolated from young animals, there was a decrease in MPCs isolated from old animals. These data suggest that T cells may play a critical role in mediating MPC function. Furthermore, aging may alter T cell-induced MPC function. These findings have implications for developing strategies aimed at increasing MPC migration and proliferation leading to an improved regenerative capacity of aged skeletal muscle.

scholarly journals Age-Related Gene Alteration in Naïve and Memory T cells Using Precise Age-Tracking Model

2021 ◽  
Vol 8 ◽  
Author(s):  
Xiaofeng Yang ◽  
Xin Wang ◽  
Lei Lei ◽  
Lina Sun ◽  
Anjun Jiao ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
T Cell ◽  
Cell Function ◽  
Memory T Cells ◽  
T Cell Subsets ◽  
Transcriptional Analysis ◽  
Immune Checkpoints ◽  
Age Related ◽  
Tracking Model

In aged individuals, age-related changes in immune cells, especially T cell deficiency, are associated with an increased incidence of infection, tumor, and autoimmune disease, as well as an impaired response to vaccination. However, the features of gene expression levels in aged T cells are still unknown. Our previous study successfully tracked aged T cells generated from one wave of developing thymocytes of young age by a lineage-specific and inducible Cre-controlled reporter (TCRδCreERR26ZsGreen mouse strain). In this study, we utilized this model and genome-wide transcriptomic analysis to examine changes in gene expression in aged naïve and memory T cell populations during the aging process. We identified profound gene alterations in aged CD4 and CD8 T cells. Both aged CD4+ and CD8+ naïve T cells showed significantly decreased organelle function. Importantly, genes associated with lymphocyte activation and function demonstrated a significant increase in aged memory T cells, accompanied by upregulation of immunosuppressive markers and immune checkpoints, revealing an abnormal T cell function in aged cells. Furthermore, aging significantly affects T cell survival and death signaling. While aged CD4 memory T cells exhibited pro-apoptotic gene signatures, aged CD8 memory T cells expressed anti-apoptotic genes. Thus, the transcriptional analysis of gene expression and signaling pathways in aged T cell subsets shed light on our understanding of altered immune function with aging, which will have great potential for clinical interventions for older adults.

scholarly journals Prevention of age-related T cell apoptosis defect in CD2-fas-transgenic mice.

1995 ◽  
Vol 182 (1) ◽  
pp. 129-137 ◽  
Author(s):  
T Zhou ◽  
C K Edwards ◽  
J D Mountz
Keyword(s):  
T Cells ◽  
T Cell ◽  
Transgenic Mice ◽  
Cell Function ◽  
Thymic Involution ◽  
Age Related ◽  
Induced Apoptosis ◽  
And Function ◽  
Old Mice ◽  
Young Mice

T cell dysfunction and thymic involution are major immunologic abnormalities associated with aging. Fas (CD95) is a bifunctional molecule that is critical for apoptosis and stimulation during T cell development, but the role of Fas during aging has not been determined. Fas expression and function on T cells from old (22-26-mo-old) mice was compared with young (2-mo-old) mice and old CD2-fas-transgenic mice. Fas expression and ligand-induced apoptosis were decreased on T cells from old mice compared with young mice. This correlated with an age-related increase in CD44+Fas- T cells. There was a marked decrease in the proliferation of T cells from old mice after anti-CD3 stimulation compared with young mice. Anti-CD3-stimulated T cells from young mice exhibited increased production of interleukin (IL)-2 and decreased production of interferon-gamma and IL-10 compared with old mice. There was an age-related decrease in the total thymocyte count from 127 +/- 10 cells in young mice compared with 26 +/- 8 x 10(6) in old mice. In 26-mo-old CD2-fas-transgenic mice, Fas and CD44 expression, Fas-induced apoptosis, T cell proliferation, and cytokine production were comparable to that of the young mice. These results suggest that T cell senescence with age is associated with defective apoptosis, and that the CD2-fas transgene allows maintenance of Fas apoptosis function and T cell function in aged mice comparable to that of young mice.

scholarly journals The Dose-Effect Safety Profile of Skeletal Muscle Precursor Cell Therapy in a Dog Model of Intrinsic Urinary Sphincter Deficiency

2015 ◽  
Vol 4 (3) ◽  
pp. 286-294 ◽  
Author(s):  
J. Koudy Williams ◽  
Delrae Eckman ◽  
Ashley Dean ◽  
Mahmoudreza Moradi ◽  
Julie Allickson ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Therapy ◽  
Safety Profile ◽  
Precursor Cell ◽  
Dose Effect ◽  
Muscle Precursor ◽  
Dog Model ◽  
Urinary Sphincter ◽  
Muscle Precursor Cell ◽  
Sphincter Deficiency

scholarly journals Sirt1 increases skeletal muscle precursor cell proliferation

2009 ◽  
Vol 88 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Christopher R. Rathbone ◽  
Frank W. Booth ◽  
Simon J. Lees
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Precursor Cell ◽  
Muscle Precursor ◽  
Muscle Precursor Cell

scholarly journals Newly generated CD4 T cells in aged animals do not exhibit age-related defects in response to antigen

2005 ◽  
Vol 201 (6) ◽  
pp. 845-851 ◽  
Author(s):  
Laura Haynes ◽  
Sheri M. Eaton ◽  
Eve M. Burns ◽  
Troy D. Randall ◽  
Susan L. Swain
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Cell Function ◽  
Ex Vivo ◽  
Cell Receptor ◽  
Older Individuals ◽  
Aged Mice ◽  
Age Related

Using a T cell receptor transgenic (TCR Tg) mouse model, we have shown that TCR Tg CD4 cells from aged mice retain a naive phenotype, but exhibit reduced proliferation and IL-2 production in response to the antigen compared with cells from young mice. We hypothesize that age-related decreases in T cell function may be partly related to the age of the T cells. Because thymic output is decreased with age, peripheral T cells in older individuals are likely to be older than those in younger individuals. To investigate this possibility, we have manipulated the age of CD4 T cells in the periphery of young and aged mice. The production of new T cells was induced by depleting peripheral CD4 T cells or by creating bone marrow chimeras. In both young and aged individuals where we induced the production of new T cells, these newly generated cells exhibited robust responses to antigen ex vivo and in vivo, exhibiting good expansion, IL-2 production, and cognate helper function. Our results suggest that age-related defects in response to antigenic stimulation, in part, are caused by the age of the CD4 T cells.

Optimization of human skeletal muscle precursor cell culture and myofiber formation in vitro

Methods ◽  
2009 ◽  
Vol 47 (2) ◽  
pp. 98-103 ◽  
Author(s):  
Daniel Eberli ◽  
Shay Soker ◽  
Anthony Atala ◽  
James J. Yoo
Keyword(s):  
Skeletal Muscle ◽  
Cell Culture ◽  
Human Skeletal Muscle ◽  
Precursor Cell ◽  
Muscle Precursor ◽  
Muscle Precursor Cell
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