The Chalkboard: The Glass pH Electrode

2004 ◽  
Vol 13 (2) ◽  
pp. 19-20
Author(s):  
Petr Vanysek
1985 ◽  
Vol 59 (5) ◽  
pp. 1660-1664
Author(s):  
S. A. Katz ◽  
A. C. Roth ◽  
E. O. Feigl

An electrode and cuvette system has been developed for the continuous and rapid measurement of either blood CO2 tension or pH. The CO2 electrode consists of a 1.5-mm-diameter flat-tip glass pH electrode covered by a film of carbonic anhydrase solution, over which a 25-micron-thick dimethyl silicone membrane is attached. Porous ceramic filled with 20% polyacrylamide, equilibrated with a salt solution, serves as a salt bridge between a Ag-AgCl reference electrode and the pH electrode surface. The electrode is housed in a four-port cuvette assembly. Blood from a vessel of interest is delivered to the cuvette by means of an occlusive roller pump. The cuvette maintains the electrode and blood at a constant temperature and directs a continuous jet of blood against the electrode surface. The cuvette also allows for easy and frequent calibration of the electrode with either gas or liquid standards. The 90% response time of the CO2 electrode is 3.0 s for liquids and 1.3 s for gases. Removal of the dimethyl silicone membrane and carbonic anhydrase film yields a pH electrode that can continuously measure blood pH with a 90% response time of 1.6 s.


1966 ◽  
Vol 21 (3) ◽  
pp. 1103-1107 ◽  
Author(s):  
G R Kelman ◽  
A J Coleman ◽  
J F Nunn

1981 ◽  
Vol 53 (3) ◽  
pp. 552-554 ◽  
Author(s):  
R. F. Savinell ◽  
C. C. Liu ◽  
T. E. Kowalsky ◽  
J. B. Puschett

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