A one-handed multi-touch mating method for 3d rotations

2013 ◽  
Author(s):  
Doug Scheurich ◽  
Wolfgang Stuerzlinger
Keyword(s):  
Mating Method

COMPARISON OF ARTIFICIAL INSEMINATION AND NATURAL MATING ON REPRODUCTIVE PERFORMANCE OF FIVE STRAINS OF SHEEP DURING THE ANESTROUS SEASON IN AN INTENSIVE SYSTEM

1979 ◽  
Vol 59 (4) ◽  
pp. 675-683 ◽  
Author(s):  
A. J. HACKETT ◽  
H. A. ROBERTSON ◽  
E. K. INSKEEP ◽  
J. N. B. SHRESTHA ◽  
M. S. WOLYNETZ
Keyword(s):  
Reproductive Performance ◽  
Artificial Insemination ◽  
Hormone Treatment ◽  
Corpora Lutea ◽  
Blood Samples ◽  
Natural Mating ◽  
Main Effects ◽  
Mating Method ◽  
Intensive System

Synchronized estrus and ovulation were induced during the anestrous season (April–May 1974) in 373 ewes of three synthetic (one sire and two dam) strains and two unselected (Suffolk and Finnish Landrace) purebred strains by treatment with 30 mg fluorogestone acetate (FGA) impregnated in polyurethane intravaginal sponges for 12 days. Following sponge removal each ewe received 500 IU pregnant mares’ serum gonadotrophin (PMSG) IM. Of these, 167 were bred by artificial insemination (AI) at 48 and 60 h post sponge removal with 0.2 ml raw unextended semen collected by electroejaculation (EE). Five days after AI, ewes were exposed to a follow up ram for 16 days for subsequent mating if a second estrus occurred. The remaining 206 were exposed to rams for a period of 22 days for natural mating. Blood samples were collected from 69 ewes, 9, 19 and 27 days post sponge removal and analyzed for progesterone to ascertain if corpora lutea were formed and whether the ewes recycled. The age of ram by mating method interaction significantly affected both fertility and fecundity mainly because some of the younger rams lacked libido and experience for natural mating. There were no significant differences in prolificacy due to any of the main effects tested. Among the 69 ewes examined for progesterone levels, 93% had formed corpora lutea after hormone treatment and 16% recycled. Only 16 of the 255 ewes that did not conceive to the synchronized estrus lambed to the subsequent estrus.

scholarly journals Transfer of Antibiotic Resistance Marker Genes between Lactic Acid Bacteria in Model Rumen and Plant Environments

2009 ◽  
Vol 75 (10) ◽  
pp. 3146-3152 ◽  
Author(s):  
Niamh Toomey ◽  
�ine Monaghan ◽  
S�amus Fanning ◽  
Declan Bolton
Keyword(s):  
Antibiotic Resistance ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Marker Genes ◽  
Natural Environments ◽  
In Vivo Models ◽  
Filter Mating ◽  
Mating Method

ABSTRACT Three wild-type dairy isolates of lactic acid bacteria (LAB) and one Lactococcus lactis control strain were analyzed for their ability to transfer antibiotic resistance determinants (plasmid or transposon located) to two LAB recipients using both in vitro methods and in vivo models. In vitro transfer experiments were carried out with the donors and recipients using the filter mating method. In vivo mating examined transfer in two natural environments, a rumen model and an alfalfa sprout model. All transconjugants were confirmed by Etest, PCR, pulsed-field gel electrophoresis, and Southern blotting. The in vitro filter mating method demonstrated high transfer frequencies between all LAB pairs, ranging from 1.8 � 10−5 to 2.2 � 10−2 transconjugants per recipient. Transconjugants were detected in the rumen model for all mating pairs tested; however, the frequencies of transfer were low and inconsistent over 48 h (ranging from 1.0 � 10−9 to 8.0 � 10−6 transconjugants per recipient). The plant model provided an environment that appeared to promote comparatively higher transfer frequencies between all LAB pairs tested over the 9-day period (transfer frequencies ranged from 4.7 � 10−4 to 3.9 � 10−1 transconjugants per recipient). In our test models, dairy cultures of LAB can act as a source of mobile genetic elements encoding antibiotic resistance that can spread to other LAB. This observation could have food safety and public health implications.

scholarly journals Combining Ability of Sixteen U.S.A. Maize Inbred Lines and Utilization Potential as Hybrids in P.R.C

2018 ◽  
Author(s):  
Jiying Sun ◽  
Julin Gao ◽  
Xiaofang Yu ◽  
Jian Liu ◽  
Zhijun Su ◽  
...  
Keyword(s):  
Combining Ability ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Corn Production ◽  
Maize Inbred Lines ◽  
American Maize ◽  
Utilization Potential ◽  
Mating Method ◽  
Future Utilization ◽  
Better Than

In China, there are increasing needs for greater genetic diversity of maize (Zea mays L.) germplasm and for hybrids appropriate for machine harvesting. to test and distinguish American maize inbred lines with exceptional combining ability, Four Chinese maize inbred lines (Chang7-2, Zheng 58,four-144 and four-287) were used to judge combining ability and heterosis of 16 U.S.A inbred lines by a NCII genetic mating method. The result showed: Among the American inbred lines, 6M502A,LH208,NL001,LH212Ht,PHW51,FBLA and LH181 showed excellent GCA for yield characteristics; while RS710,PHP76,FBLA,and PHJ89 showed excellent GCA for machine harvesting characteristics. In hybrid combinations, NL001×Chang7-2,LH212Ht×Chang7-2,FBLA×four-144,LH181×four-287,PHK93×four-287 had better SCA for yield characteristics; while NL001×Chang 7-2,6M502A×Chang7-2,LH212Ht×Chang7-2,LH181×four-287,PHW51×Chang7-2 were better than the check for machine harvesting characteristics. NL001×Chang7-2, 6M502A×Chang7-2,LH212Ht×Chang7-2,LH181×four-287,PHW51×Chang7-2 showed excellent total combined advantages compared to the check and potential for future utilization in Inner Mongolia corn production.

scholarly journals Effect of mating method, sex and birth type on growth of lambs

2013 ◽  
Vol 29 (2) ◽  
pp. 277-286 ◽  
Author(s):  
Zoran Ilic ◽  
A. Jevtic-Vukmirovic ◽  
M.P. Petrovic ◽  
V. Caro-Petrovic ◽  
B. Milosevic ◽  
...  
Keyword(s):  
Body Weight ◽  
Statistical Analysis ◽  
Statistical Significance ◽  
Reproductive Traits ◽  
The Body ◽  
Estrus Synchronization ◽  
Body Weights ◽  
The Difference ◽  
Mating Method ◽  
Birth Type

Estrus synchronization methods was use to control the reproductive traits of sheep, as well as bringing more females at the same stage of estrus and ovulation. According to the points mentioned above, the aim of the present study was to investigate and compare mating method and influence of fixed factors on birth and weaning weight of lambs. Statistical analysis showed that exist difference in the body weights between genotypes of lambs. In the first group, the difference in weight of lambs at birth, regardless of mating method was not significant (P> 0.05), while in the second group, the difference was slightly significant (P <0.05). As for the birth type, sex and within the same genotype, there was a statistical significance (P <0.05) between singles obtained naturally, between the triplets obtained naturally and between triplets received hormonal method. All the differences between body weight at 30 days (mating method, sex and birth type under the same genotype) were statistically significant (P <0.05).The determined differences in the body weights at 60 days (sex, mating method and type of birth under the same genotype) were statistically significant (P <0.05). The results showed that the differences (mating method, sex and type of birth under the same genotype) were statistically significant (P <0.05), except in between body weight at 90 days in twins among two genotypes obtained by natural method, which were not statistically significant (P> 0.05).

scholarly journals Selective Hybridization between Sake Yeast and Wine Yeast by Cell-Spore Mating Method

1989 ◽  
Vol 84 (6) ◽  
pp. 407-412
Author(s):  
Yoshinobu TANI ◽  
Yukio TOMOHIRO ◽  
Akira MIYATA ◽  
Kazuyoshi OHTA ◽  
Shinsaku HAYASHIDA
Keyword(s):  
Wine Yeast ◽  
Selective Hybridization ◽  
Mating Method

Optimal packing using the multiple mating method

2003 ◽  
Vol 19 (1) ◽  
pp. 56-65 ◽  
Author(s):  
C. Yu ◽  
S. Manoochehri
Keyword(s):  
Multiple Mating ◽  
Optimal Packing ◽  
Mating Method

scholarly journals BINARY VECTOR CONSTRUCTION OF KAPPA(κ)-CARRAGEENASE GENE AND TRANSFORMATION TO Agrobacterium tumefaciens AS MEDIATOR FOR SEAWEED TRANSGENIC GENERATION

2016 ◽  
Vol 8 (1) ◽  
pp. 335-344
Author(s):  
Muh Alias L. Rajamuddin ◽  
. Alimuddin ◽  
Utut Widyastuti ◽  
Enang Harris ◽  
Emma Suryati
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Gene Sequence ◽  
Kappaphycus Alvarezii ◽  
Pcr Analysis ◽  
E Coli ◽  
Vector Construction ◽  
Dna Fragment ◽  
Dna Template ◽  
Parental Mating ◽  
Mating Method

Increasing of kappa (κ)-carrageenan content in Kappaphycus alvarezii seaweed is potentially be achieved by applying transgenesis technology. This study was performed to obtain a construction of  κ-Carrageenase gene and Agrobacterium tumefaciens to carry those construction genes.  The κ-Carrageenase (κ-Car) gene was involved in κ-carrageenan biosynthesis. The κ-Car gene sequence was ligated between the 35S CaMV promoter and tNos terminator sequences to generate pMSH/κ-Car expression vector. Transformation of pMSH/κ-Car plasmid to Escherichia coli was performed by heat-shock method, and to Agrobacterium tumefaciens by tri-parental mating method. The results showed that several colonies of E. coli and A. tumefaciens grew in the selective culture mediums containing antibiotic. PCR analysis using primers 35S-Forward and tNos-Reverse with DNA template from those bacterial colonies resulted DNA fragment of about 2,000 bp, the same as the total length of 35S CaMV promoter, κ-Car gene and tNos terminator sequences. Therefore, the construction of pMSH/κ-Car gene was succeeded and a colony of A. tumefaciens transformant carrying pMSH/κ-Car plasmid was successfully produced.                                                                                   Keywords:  Agrobacterium tumefaciens, kappa(κ)-Carrageenase gene, transgenesis, vector

Effects of Antifertility Agents on Male Mice as Determined by a Serial Mating Method

1969 ◽  
Vol 131 (2) ◽  
pp. 667-669 ◽  
Author(s):  
L. G. Hershberger ◽  
D. M. Hansen ◽  
L. M. Hansen
Keyword(s):  
Male Mice ◽  
Mating Method
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