scholarly journals A Rigorous Justification of the Point B Method for Determination of Monolayer Capacity from a Type II Adsorption Isotherm

1975 ◽  
Vol 53 (1) ◽  
pp. 21-40 ◽  
Author(s):  
S.-T. Wu
Keyword(s):  
Adsorption Isotherm ◽  
Type Ii ◽  
Monolayer Capacity ◽  
B Method

Human Umbilical Cord Mesenchymal Stem Cells Induce into Chondrocytes in 3D Cultured System

2013 ◽  
Vol 749 ◽  
pp. 198-205
Author(s):  
Li Yu ◽  
Jing Liu ◽  
Chao Xu ◽  
Er Mei Luo ◽  
Ming Qiao Tang
Keyword(s):  
Real Time ◽  
Alcian Blue ◽  
Culture System ◽  
Type Ii Collagen ◽  
Human Umbilical Cord ◽  
Type Ii ◽  
Pellet Culture ◽  
Hla Dr

Objective: To investigate a better method of inducing hUC-MSCs into chondrocytes in different culture system in vitro. Method: hUC-MSCs were isolated and cultured by tissue block culture, and the cells surface antigens were identified by flow cytometry, hUC-MSCs were cultured with chondrogenic media and stained with Alcian Blue. The production of matrix was estimated from the determination of hydroxyproline content and Alcian Blue method. Expressions of glycosaminoglycan (GAG), type II collagen and Sox-9 were assayed by real-time fluorescence quantitative PCR. Results: The cultured hUC-MSCs phenotype was CD105+/CD29+/CD44+/ CD31-/CD34-/ CD40-/CD45-/HLA-DR-. hUC-MSCs weakly expressed chondrocyte marker, which strongly expressed GAG and type II collagen after chondrogenic induction, and the cells were incubated in pellet culture with higher expression. Real-time PCR results demonstrated that chondrogenic induction cells were expressed GAG, type II collagen and Sox-9, and the cells were incubated in pellet culture with higher expression. Conclusion: hUC-MSCs incubated in pellet culture is more conducive to differentiate into chondrocytes than those cultured in monolayer culture system.

scholarly journals Determination of free L-carnitine levels in type II diabetic women with and without complications

2007 ◽  
Vol 61 (7) ◽  
pp. 892-895 ◽  
Author(s):  
A Poorabbas ◽  
F Fallah ◽  
J Bagdadchi ◽  
R Mahdavi ◽  
A Aliasgarzadeh ◽  
...  
Keyword(s):  
Type Ii

Administration of a Mixture of Fungal Glucosidases to a Patient with Type II Glycogenosis (Pompe's Disease)

PEDIATRICS ◽  
1968 ◽  
Vol 42 (4) ◽  
pp. 672-676
Author(s):  
Ronald M. Lauer ◽  
Thelma Mascarinas ◽  
Antonio S. Racela ◽  
Antoni M. Diehl ◽  
Barbara Illingworth Brown
Keyword(s):  
Skeletal Muscle ◽  
Enzyme Assay ◽  
Glycogen Content ◽  
Hydrolytic Enzymes ◽  
Type Ii ◽  
Electron Microscopic ◽  
Pompe’S Disease ◽  
Pompe's Disease ◽  
Type Ii Glycogenosis

A case of Type II glycogenosis (Pompe's disease) has been studied by histochemical, electron microscopic, and biochemical techniques. These studies have been made prior to and after the intramuscular administration for 1 week of a mixture of hydrolytic enzymes containing both α-1,4- and α-1,6-glucosidase activities. Electron photomicrographs of the liver before enzyme administration showed glycogen to be located both within and outside of membrane-limited vacuoles. No change in this distribution could be detected in tissue removed by biopsy after enzyme administration. This impression was confirmed by the determination of glycogen content which was shown to be unchanged. Nevertheless, the liver was found by enzyme assay to contain the administered enzyme. Leucocytes isolated from blood taken 4 hours after the last enzyme injection were also shown to contain the parenterally administered glucosidases. In skeletal muscle glycogen was present chiefly as extrasaccular deposits which were unchanged in appearance by enzyme administration. No glucosidase activity was demonstrable in the skeletal muscle after such a treatment. Myocardium sectioned after autopsy had major deposits of glycogen in extrasaccular areas.

scholarly journals DETERMINATION OF CROSS SECTIONS FOR GRAVITY TYPE QUAY WALLS

2017 ◽  
pp. 5
Author(s):  
Tugce Yuksel ◽  
Yalcin Yuksel ◽  
Busra Basaran ◽  
Esin Cevik
Keyword(s):  
Cross Sections ◽  
Vertical Wall ◽  
Block Size ◽  
Seismic Loading ◽  
Block Type ◽  
Type I ◽  
Type Ii ◽  
Seismic Loadings ◽  
Port Structures

Block type quay walls are widely used as port structures in the world. In this study three types of vertical block type quay walls with different block size exposed to seismic loading were investigated experimentally. The block ratios of Type I, Type II and III vertical wall models are B/h=2, 1.5 and 1.5 & H/h=6, 6 and 3, respectively. The tests were conducted in the shaking tank with different harmonic seismic loadings and the behaviors of these walls were investigated comparatively.

The Determination of Macrostresses (Type-I Stresses) and Microstresses (Type-Ii Stresses) by Neutron Diffraction

1994 ◽  
Vol 376 ◽  
Author(s):  
T.M. Holden
Keyword(s):  
Neutron Diffraction ◽  
Experimental Methods ◽  
Stress Fields ◽  
Type I ◽  
Type Ii ◽  
Sources Of Error ◽  
Precision And Accuracy

ABSTRACTNeutron diffraction has been used as tool for measuring stress fields (type-I stresses) and intergranular or microstresses (type-II stresses) for a decade. The experimental methods for measuring the two types of stresses are outlined. Emphasis is placed on maximizing the precision and accuracy of the measurements. Some serious sources of error in measurements near surfaces and some subtle wavelength effects are considered.

Baade-Wesselink Radius Determination of Type II Cepheids

1997 ◽  
Vol 113 ◽  
pp. 1833 ◽  
Author(s):  
Z. Balog ◽  
J. Vinko ◽  
G. Kaszas
Keyword(s):  
Type Ii
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