Treatment of experimental alveolar echinococcosis with albendazole: a 1H NMR spectroscopic study

The Meriones unguiculatus (jird) – Echinococcus multilocularis host–parasite model was used to evaluate metabolic changes in livers and cysts treated with albendazole (ABZ). Hydrogen-1 nuclear magnetic resonance spectra of liver extracts showed that livers of uninfected jirds fed olive oil contained more glycine but less glycerophosphocholine (GPC) than those of uninfected untreated controls. When ABZ was added to the olive oil and tested on uninfected animals, other modifications in the metabolic profile of the liver could be seen. While the concentration of GPC stayed reduced and that of glycine reverted to normal, the levels of glycogen, phosphocreatine/creatine (PCr/Cr), succinate, and acetate were lower than those in the normal liver. The metabolic pathology in this organ was further magnified in jirds infected with E. multilocularis. In this case, the liver was depleted of glycogen, glucose, taurine, GPC, and acetate, but had more betaine, phosphocholine, choline, PCr/Cr, and succinate. These abnormalities were markedly diminished by ABZ treatment, with only glycogen, GPC, and acetate concentrations being low when the treatment was terminated. Also, the drug suppressed the growth of the parasite by 75%, and extracts of these parasite cysts contained less glycogen, glycine, succinate, acetate, and alanine but more taurine, GPC, and PCr/Cr than extracts of those from untreated hosts.