Metabolism of D-[1-13C]glucose in livers of Meriones unguiculatus infected with Echinococcus multilocularis

1995 ◽  
Vol 73 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Marie Novak ◽  
Ashok Modha ◽  
Jonathan Lee ◽  
Richard Buist ◽  
Barry Blackburn
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Quantitative Evaluation ◽  
Perchloric Acid ◽  
Echinococcus Multilocularis ◽  
Glycogen Synthesis ◽  
Meriones Unguiculatus ◽  
Nmr Analysis ◽  
In Situ Spectra

Following administration of [1-13C]glucose, sequential 13C nuclear magnetic resonance (NMR) in situ spectra were obtained from the liver of uninfected jirds (Meriones unguiculatus) and those infected with Echinococcus multilocularis over a period of 2 h. Quantitative evaluation of the flow of labelled carbon through the liver at 80 and 120 min after glucose administration revealed that although the percentage of labelled glucose utilized by the liver was the same for both groups, glycogen synthesis differed. At both times, the livers of infected animals had incorporated a smaller percentage of the [1-13C]glucose into glycogen labelled at C1 and a larger percentage into the C6 position of glucose/glycogen. In another experiment, identical with respect to the substrate administered, NMR analysis of perchloric acid extracts revealed that the livers of infected animals had lower concentrations of labelled glucose and glycogen and higher concentrations of labelled alanine and lactate than those of uninfected controls. Concentration differences were also noted for some of the unlabelled metabolites. Echinococcus multilocularis cysts contained the same labelled metabolites as the livers but the concentration of lactate was much higher. Parasite cysts also contained labelled acetate.

Treatment of experimental alveolar echinococcosis with albendazole: a 1H NMR spectroscopic study

1997 ◽  
Vol 75 (2) ◽  
pp. 198-204 ◽  
Author(s):  
A. Modha ◽  
M. Novak ◽  
B. J. Blackburn
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Olive Oil ◽  
Echinococcus Multilocularis ◽  
Metabolic Profile ◽  
Alveolar Echinococcosis ◽  
Normal Liver ◽  
Meriones Unguiculatus ◽  
Nuclear Magnetic Resonance Spectra ◽  
Host Parasite

The Meriones unguiculatus (jird) – Echinococcus multilocularis host–parasite model was used to evaluate metabolic changes in livers and cysts treated with albendazole (ABZ). Hydrogen-1 nuclear magnetic resonance spectra of liver extracts showed that livers of uninfected jirds fed olive oil contained more glycine but less glycerophosphocholine (GPC) than those of uninfected untreated controls. When ABZ was added to the olive oil and tested on uninfected animals, other modifications in the metabolic profile of the liver could be seen. While the concentration of GPC stayed reduced and that of glycine reverted to normal, the levels of glycogen, phosphocreatine/creatine (PCr/Cr), succinate, and acetate were lower than those in the normal liver. The metabolic pathology in this organ was further magnified in jirds infected with E. multilocularis. In this case, the liver was depleted of glycogen, glucose, taurine, GPC, and acetate, but had more betaine, phosphocholine, choline, PCr/Cr, and succinate. These abnormalities were markedly diminished by ABZ treatment, with only glycogen, GPC, and acetate concentrations being low when the treatment was terminated. Also, the drug suppressed the growth of the parasite by 75%, and extracts of these parasite cysts contained less glycogen, glycine, succinate, acetate, and alanine but more taurine, GPC, and PCr/Cr than extracts of those from untreated hosts.

In situ enzymatic removal of orthophosphate by the nucleoside phosphorylase catalyzed phosphorolysis of nicotinamide riboside

1982 ◽  
Vol 60 (9) ◽  
pp. 917-921 ◽  
Author(s):  
John W. Shriver ◽  
Brian D. Sykes
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nucleoside Phosphorylase ◽  
Nicotinamide Riboside ◽  
Time Required ◽  
Removal System ◽  
Nuclear Magnetic

An enzymatic orthophosphate removal system is described which can be effectively used to continuously remove orthophosphate from biochemical samples. The phosphorolysis of nicotinamide riboside is catalyzed by calf spleen nucleoside phosphorylase to give ribose-1-PO4 and nicotinamide along with a proton. At pH 8 the production of ribose-1-PO4 from orthophosphate is essentially quantitative. This reaction can be monitored optically or by 31P nuclear magnetic resonance (NMR). Equations are given for determining the time required to remove a given amount of phosphate from a typical NMR sample with a known amount of nucleoside phosphorylase. The effects of a competing orthophosphate-producing reaction are considered.

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