In the first part of this paper I described the life-history, spermatogenesis, maturation of the fertilised egg, fertilisation and segmentation divisions of the summer generation of the gall-fly
Neuroterus lenticularis
(
Spathegaster baccarum
), and gave some observations on the maturation of the eggs of the spring generation, and on the somatic mitoses in males and females. These latter observations were admittedly incomplete owing to lack of material, and this second part of the paper attempts to complete the account of what was left uncertain in the first communication. Before proceeding to describe my fresh observations it may be well shortly to summarise the results arrived at in the fist part. The spring generation of
N. lenticularis
consists of females which lay parthenogenetic eggs; those laid by some females develop into sexual females, both of which hatch in June. The eggs of the summer females undergo a double maturation division, leaving 10 chromosomes in the nucleus of the mature egg. The spermatogonia of the males in the same generation have 10 chromosomes; the first spermatocyte division is suppressed; in the second spermatocyte division the chromosomes divide, 10 entering each spermatid. A small staining extra-nuclear body found in the spermatocytes does not divide, and is included in only half of the spermatids. In fertilisation, the egg and sperm nuclei each contain 10 chromosomes, and the segmentation divisions of the fertilised egg show 20. For a complete study of the maturation and segmentation of the parthenogenetic spring eggs my material was insufficient, but it suggested that the eggs laid by some females underwent maturation and showed 10 chromosomes in the segmentation divisions, and that others had no polar divisions and showed 20. Since somatic and oogonial mitoses of the summer female have 20 chromosomes, but the spermatogonial mitoses and those of the developing nervous system in the male only 10, it was suggested that the eggs which undergo maturation produce males, the others females. An attempt was made, in conclusion, to connect these phenomena with what is know of sex-determination in other animals.