Intraspecific variation in sibling species of Simulium venustum and Simulium verecundum complexes (Diptera: Simuliidae) revealed by the sequence of the mitochondrial 16S rRNAgene

1993 ◽  
Vol 71 (6) ◽  
pp. 1202-1206 ◽  
Author(s):  
Bai Xiong ◽  
Thomas D. Kocher
Keyword(s):  
Mitochondrial Dna ◽  
Intraspecific Variation ◽  
New Hampshire ◽  
Ribosomal Rna ◽  
Sibling Species ◽  
Black Flies ◽  
Equal Frequency ◽  
Base Pairs ◽  
Three Samples ◽  
Mitochondrial Dna Haplotype

A portion of the mitochondrial large ribosomal RNA gene was amplified and sequenced (460 base pairs) for 10 individuals of each of nine samples of black flies representing three sibling species of the Simulium venustum complex (CC, CC3, and AC(gB)) and two sibling species of the Simulium verecundum complex (ACD = Simulium rostratum and AA). Mitochondrial DNA haplotype heterogeneity was observed in all but the sample of S. verecundum AA from New Hampshire. The sample of S. venustum CC3 had a significantly larger number of haplotypes than other samples. A predominant haplotype was found in most samples except S. venustum CC3 and S. venustum CC from New Hampshire (nine haplotypes in the sample of S. venustum CC3 and two haplotypes with equal frequency in the sample of S. venustum CC from New Hampshire). The predominant haplotype was the same among the three samples of S. verecundum AA. Samples of S. venustum CC also shared haplotypes, but the predominant haplotype was different in each sample. Despite intraspecific variation, haplotypes are usually clustered on a conspecific basis.

scholarly journals Molecular Genetic Status of Aleutian Canada Geese From Buldir and the Semidi Islands, Alaska

The Condor ◽  
2000 ◽  
Vol 102 (1) ◽  
pp. 172-180 ◽  
Author(s):  
Barbara J. Pierson ◽  
John M. Pearce ◽  
Sandra L. Talbot ◽  
Gerald F. Shields ◽  
Kim T. Scribner
Keyword(s):  
Mitochondrial Dna ◽  
Random Mating ◽  
Molecular Genetic ◽  
Canada Goose ◽  
Canada Geese ◽  
Population Bottlenecks ◽  
Base Pairs ◽  
Population Genetic Differentiation ◽  
Contemporary Gene Flow ◽  
Mitochondrial Dna Haplotype

Abstract We conducted genetic analyses of Aleutian Canada Geese (Branta canadensis leucopareia) from Buldir Island in the western Aleutians and the Semidi Islands in the eastern portion of their breeding range. We compared data from seven microsatellite DNA loci and 143 base pairs of the control region of mitochondrial DNA from the two populations of Aleutian Canada Geese and another small-bodied subspecies, the Cackling Canada Goose (B. c. minima) which nests in western Alaska. The widely separated island-nesting Aleutian geese were genetically more closely related to each other than to mainland-nesting small-bodied geese. The populations of Aleutian geese were genetically differentiated from one another in terms of mitochondrial DNA haplotype and microsatellite allele frequencies, suggesting limited contemporary gene flow and/or major shifts in gene frequency through genetic drift. The degree of population genetic differentiation suggests that Aleutian Canada Goose populations could be considered separate management units. There was some evidence of population bottlenecks, although we found no significant genetic evidence of non-random mating or inbreeding.

scholarly journals DNA marking of the rate of maturation of gonads in female sterlet x beluga (A. ruthenus x H. huso) hybrid under conditions of recirculation systems

2021 ◽  
Vol 937 (3) ◽  
pp. 032038
Author(s):  
P Geraskin ◽  
I Kornienko ◽  
V Grigoriev ◽  
A Kovaleva ◽  
M Mahotkin
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
New Technologies ◽  
Molecular Genetic ◽  
Base Pairs ◽  
Exact Test ◽  
D Loop ◽  
Mitochondrial Dna Haplotype ◽  
Closed Water ◽  
Conducting Research

Abstract To create a theoretical basis for the development of new technologies for the formation of highly productive sturgeon herds, work has begun on conducting research on DNA - markers associated with economically useful traits. At the first stage of the work, polymorphic regions of the mitochondrial DNA (mtDNA) D-loop were investigated in order to search for promising molecular genetic markers associated with the production properties of sturgeon hybrids. Along with point polymorphisms in mtDNA hybrids, variability in the length of the D-loop was observed, as well as the presence of heteroplasmy in length. The length variability of the D-loop is due to the presence of tandem repeating units in multiples of 80 base pairs (bp). Using Fisher’s exact test, it was shown that the proportion of individuals with four tandem repeating units of 80 bp eachsignificantly higher (p = 0.030) in the group of highly productive hybrids. The obtained data suggest that such a trait as the accelerated maturation of female hybrids (A. ruthenus x H. huso) grown in a closed water supply can be associated with the mitochondrial DNA haplotype, in the D-loop of which there are fourrepeating units.

scholarly journals First Insight into Groundwater Copepods of the Polish Lowland

Water ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2086
Author(s):  
Maciej Karpowicz ◽  
Sabina Smolska ◽  
Magdalena Świsłocka ◽  
Joanna Moroz
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Dna Markers ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Morphological Description ◽  
Single Plate ◽  
North Eastern ◽  
Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

scholarly journals Genetic Diversities and Historical Dynamics of Native Ethiopian Horse Populations (Equus caballus) Inferred from Mitochondrial DNA Polymorphisms

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 155
Author(s):  
Kefena Effa ◽  
Sonia Rosenbom ◽  
Jianlin Han ◽  
Tadelle Dessie ◽  
Albano Beja-Pereira
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Sequence Divergence ◽  
Dna Polymorphisms ◽  
Base Pairs ◽  
Feral Horses ◽  
Domestic Horses ◽  
D Loop ◽  
Diversity Estimates

Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (FST) and nucleotide sequence divergence (DA), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.

Mitochondrial DNA Haplotype Diversity in Two Portunid Populations Found along the Georgia and South Carolina Coasts

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Amy Abdulovic-Cui ◽  
Sierra Mannix ◽  
Austin Coleman ◽  
Brittany White ◽  
Keturah Mingledolph ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
South Carolina ◽  
Haplotype Diversity ◽  
Mitochondrial Dna Haplotype

Mitochondrial DNA polymorphism in a black fly, Simulium vittatum (Diptera: Simuliidae)

1998 ◽  
Vol 76 (3) ◽  
pp. 440-447 ◽  
Author(s):  
X Zhu ◽  
K P Pruess ◽  
T O Powers
Keyword(s):  
New York ◽  
Mitochondrial Dna ◽  
Rflp Analysis ◽  
Mitochondrial Haplotype ◽  
Black Flies ◽  
Mitochondrial Dna Polymorphism ◽  
Simulium Vittatum ◽  
Hybridization Probes ◽  
Restriction Sites ◽  
Mitochondrial Heterogeneity

Mitochondrial DNA (mtDNA) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70% of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five HindIII fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 HindIII restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoR1 and HindIII revealed five additional haplotypes. One HindIII haplotype was present in 84% of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.

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