scholarly journals First Insight into Groundwater Copepods of the Polish Lowland

Water ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2086
Author(s):  
Maciej Karpowicz ◽  
Sabina Smolska ◽  
Magdalena Świsłocka ◽  
Joanna Moroz
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Dna Markers ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Morphological Description ◽  
Single Plate ◽  
North Eastern ◽  
Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

scholarly journals Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 711
Author(s):  
Sixin Zhang ◽  
Guangping Huang ◽  
Liang Li ◽  
Xianyong Liu ◽  
Xiaoli Tang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Mean Intensity ◽  
Gnathostoma Spinigerum ◽  
Zoonotic Parasite ◽  
Third Stage ◽  
Parasite Infections ◽  
Asian Swamp Eel ◽  
Swamp Eel

Nematode infections transmitted to humans by the consumption of wild or cultured eels are increasingly being reported. In the present study, 120 Asian swamp eel, Monopterus albus (Zuiew), individuals collected from China were examined for parasite infections, and 78 larval nematodes were isolated. Morphological and molecular characteristics, including sequence and phylogenetic analysis of the internal transcribed spacer (ITS) and cytochrome c oxidase subunit I (COI) gene regions, were employed to identify these nematodes at the lowest taxonomic level possible. Asian swamp eel was infected with two zoonotic parasite taxa: Gnathostoma spinigerum advanced third-stage larvae, with 6.67% prevalence and mean intensity = 1.25, and Eustrongylides sp. fourth-stage larvae, with 26.67% prevalence and mean intensity = 2.13. These findings evidence the need to enhance public hygiene and food safety awareness toward eel consumption

Découverte d’une nouvelle population de Rhithrogena delphinensis Sowa & Degrange, 1987, dans le massif des Arves, et compléments à la description de la larve (Ephemeroptera, Heptageniidae)

2021 ◽  
Vol 126 (4) ◽  
pp. 401-420
Author(s):  
Bertrand Launay ◽  
Julien Barnasson ◽  
Juliette Becquet ◽  
Michel Brulin ◽  
Sophie Cauvy-Fraunie ◽  
...  
Keyword(s):  
Drainage Basin ◽  
Morphological Characteristics ◽  
Western Alps ◽  
Coi Gene ◽  
Morphological Description ◽  
Base Pairs ◽  
Northern Flank ◽  
Ecological Requirements ◽  
First Time ◽  
Existing Data

Discovery of a new population of Rhithrogena delphinensis Sowa & Degrange, 1987, in the Arves Massif, and additions to the morphological description of the larva (Ephemeroptera, Heptageniidae). Rhithrogena delphinensis, described originally on the basis of four larvae from the Western Alps, south of the Arves Massif and from the northern flank of the Ecrins Massif, had not been captured again since 1986. Here, we report the discovery of a new population from river Arvan, whose drainage basin is located between the Grandes Rousses Massif and the northern flank of the Arves Massif. This newly discovered population seems abundant in numbers, and reveals the particular ecological requirements of the species as well as its dependence on glacier fed or nival streams. The morphological characteristics of the larvae are described in detail, and illustrated by photographs. The variability of some of the proposed identification criteria is discussed, and a key to the identification of the Rhithrogena species from the alpestris group of the Western Alps, to which R. delphinensis belongs, is provided. Finally, a portion of 658 base pairs of the COI gene of R. delphinensis is sequenced for the first time and compared to already existing data on the alpestris group in the Western Alps.

scholarly journals Central European Border Settlements and Interwar Ireland: a Transnational Study of the North- Eastern Boundary Bureau and the Boundary Commission

2017 ◽  
Vol 57 (3) ◽  
Author(s):  
Lili Zách
Keyword(s):  
World War I ◽  
Central Europe ◽  
East Central Europe ◽  
Central European ◽  
The North ◽  
Eastern Boundary ◽  
North Eastern ◽  
Post War ◽  
Transnational Study ◽  
Insight Into

ABSTRACTIn the aftermath of the Great War, the birth of new independent small states in East-Central Europe was closely followed in Irish nationalist circles due to the possibility of Partition in Ireland. Newspaper editorials, journal articles and diplomatic accounts illustrate that post-war Ireland had an open attitude toward the settlement of borders on the Continent as the dissolution of the Austro-Hungarian Empire was similarly controversial. This paper aims to investigate how contemporary Irish commentators perceived the question of boundary settlements in Central Europe in order to provide an insight into the transformation of political space in both Ireland and Central Europe. After providing a brief background to the Irish boundary question, this paper touches upon the most important points in historiography with regard to border settlements in the post-World War I era.. It also discusses Irish Partition history in detail, concentrating on the North-Eastern Boundary Bureau (NEBB) and the Boundary Commission, and the importance of Central European precedents in their work. Moreover, this paper also proposes to provide an insight into the Irish interest in the minority problem in European borderland regions after 1925 in order to illustrate the outward-looking attitude to Irish nationalists, even in relation to borders and minorities.

scholarly journals Molecular Methods for the Detection of Natural Hybrids in Sturgeon Populations

2015 ◽  
Vol 16 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Alexandru Burcea ◽  
Iulia Elena Florescu ◽  
Andreea Dudu ◽  
Sergiu Emil Georgescu ◽  
Marieta Costache
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
Molecular Methods ◽  
Hybrid Species ◽  
Habitat Area ◽  
Natural Hybrids ◽  
Sturgeon Species ◽  
Morphological Criteria ◽  
Hybrid Sturgeon

Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

scholarly journals A phylogenetic framework for the bumblebee species of the subgenus Bombus sensu stricto based on mitochondrial DNA markers, with a short description of the neglected taxon B. minshanicola Bischoff, 1936 n. status. (Hymenoptera: Apidae: Bombus).

2010 ◽  
Vol 60 (2) ◽  
pp. 471-487
Author(s):  
Andreas Bertsch
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Dna Markers ◽  
Sensu Stricto ◽  
Short Description ◽  
Bumblebee Species ◽  
Neue Art ◽  
Phylogenetic Framework ◽  
Bombus Affinis ◽  
N Status

Königinnen von 12 Taxa der Untergattung Bombus sensu stricto (Bombus affinis, B. albocinctus, B. cryptarum, B. lucorum, B. magnus, B. moderatus, B. occidentalis, B. patagiatus, B. sporadicus, B. terrestris, B. terricola and B. tunicatus) wurden im Frühjahr gefangen, um künstliche Kolonien zu züchten. Zusätzlich wurden Männchen von B. franklini und B. lucorum/China gefangen. Mitochondriale Cytochrome Oxidase Untereinheit I (COI) von 53 Proben wurde sequenziert (Teilsequenzen der Länge 1257 bp). GenBank Sequenzen der Ostasiatischen Arten B. hypocrita, B. ignitus und B. lucorum/China wurden einbezogen. Der Unterschied zwischen den Arten beträgt 30 – 92 Basen-Substitutionen und die Tamura-Nei genetische Distanz 0.030-0.103, während der Unterschied innerhalb der Arten nur 1 – 3 Basensubstitution beträgt und die Tamura-Nei genetische Distanz nur 0.001-0.003. Dreizehn Taxa mit Artrang sowie eine neue Art B. minshanicola Bischoff 1936 (= B. terrestris ssp. minshanicola = B. lucorum/China) wurden nachgewiesen. In der Topologie des phylogenetischen Stammbaums gibt es 4 Cluster: ein Artenpaar B. sporadicus – B. ignitus und ein Artenpaar B. terricola – B. occidentalis, ein Cluster für B. magnus, B. patagiatus and B. cryptarum mit den Subspecies B. cryptarum albocinctus und B. cryptarum moderatus sowie ein Cluster für B. hypocrita, B. lucorum, B. minshanicola, B. franklini and B. affinis. Die Arten B. terrestris mit den Subspecies B. terrestris canariensis und B. terrestris sassaricus sowie B. tunicatus sind einzeln abgetrennt. Da sich im Alignment der COI Sequenzen keine Lücken finden, können die einzelnen Nucleotid-Positionen als homolog betrachtet werden. Jede Art besitzt eine Reihe einzigartiger (‘privater’) Positionen, die als diagnostische Merkmale benutzt werden können um die Art zu definieren und zu identifizieren.

First pentasetacid mite from Australasian Araucariaceae: morphological description and molecular phylogenetic position of Pentasetacus novozelandicus n. sp. (Eriophyoidea, Pentasetacidae) and remarks on anal lobes in eriophyoid mites

2019 ◽  
Vol 24 (7) ◽  
pp. 1284-1309 ◽  
Author(s):  
Philipp Chetverikov ◽  
C. CRAEMER C. CRAEMER ◽  
T. CVRKOVIĆ T. CVRKOVIĆ ◽  
P.G. EFIMOV P.G. EFIMOV ◽  
P.B. KLIMOV P.B. KLIMOV ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Phylogenetic Analyses ◽  
Mite Species ◽  
Laser Scanning Microscopy ◽  
Coi Gene ◽  
Confocal Laser ◽  
Phylogenetic Position ◽  
Morphological Description ◽  
Eriophyoid Mites ◽  
Molecular Phylogenetic

A new vagrant eriophyoid mite species of the archaic genus Pentasetacus (Schliesske 1985), P. novozelandicus n. sp., is described with the aid of conventional microscopy, confocal laser scanning microscopy and scanning electron microscopy. It was found on Araucaria heterophylla, which is an araucarian that is endemic to Norfolk Island and introduced to New Zealand. Partial sequences of mitochondrial barcode COI gene and D1–D2 domains of nuclear rDNA of two pentasetacid mites, P. araucariae (MK903025 and MK898944) and P. novozelandicus n. sp. (MK903024 and MK898943) are provided. Molecular phylogenetic analyses of full-length D1–D2 eriophyoid sequences, including GenBank sequences and newly generated sequences of pentasetacids, confirmed the monophyly of Pentasetacidae but failed to resolve the basal phylogeny of Eriophyoidea. This may be because the D1–D2 domains of 28S are hypervariable in Eriophyoidea. Moreover, in pentasetacids D1–D2 sequences are about 20% shorter than in other eriophyoids, and thus harder to align. Two types of anal lobes are described in Eriophyoidea: (1) Eriophyidae s.l. and Phytoptidae s.l. have bilaterally symmetric lobes; (2) pentasetacids have non-divided lobes. The presence of an anal secretory apparatus, comprising internal structures that have previously been described in Eriophyidae s.l. and Phytoptidae s.l., is confirmed in pentasetacid genera. The phylogeny of pentasetacids is also discussed in the context of the paleobiography of Araucariaceae.

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