The azurocyte: a new kind of leukocyte from wild voles (Microtus)

1987 ◽  
Vol 65 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Steve Mihok ◽  
Jean-Paul Descôteaux ◽  
Todd Lawton ◽  
Anya Lobreau ◽  
Bill Schwartz
Keyword(s):  
Special Functions ◽  
Natural Killer Activity ◽  
Nonspecific Esterase ◽  
Cytoplasmic Inclusions ◽  
Killer Activity ◽  
Pregnant Females ◽  
Large Mononuclear Cell ◽  
Membrane Bound ◽  
Bound Electron ◽  
Sulphated Glycosaminoglycan

We have identified a new mammalian leukocyte from voles (Cricetidae, Microtus). This "azurocyte" is a large mononuclear cell with numerous cytoplasmic inclusions that stain purple with azure dyes. The cell resembles a young lymphocyte with numerous mitochondria, a prominent Golgi complex, and modest amounts of rough endoplasmic reticulum. The inclusions are homogeneous, membrane-bound, electron-dense bodies that contain a highly acidic, sulphated glycosaminoglycan, glycoprotein, nonspecific esterase, β-glucuronidase, and an aminopeptidase. Azurocytes are common in adult voles, particularly pregnant females, and can be induced artificially with progesterone. In humans (large granular lymphocyte) and in guinea pigs (Kurloff cell), morphologically distinct cells with azurophilic inclusions have been associated with natural killer activity. The vole azurocyte may therefore be a similar leukocyte with special functions during pregnancy.

scholarly journals Natural killer and suppressor T-cell chronic lymphocytic leukemia

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 627-634 ◽  
Author(s):  
M Palutke ◽  
L Eisenberg ◽  
J Kaplan ◽  
M Hussain ◽  
K Kithier ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chronic Lymphocytic Leukemia ◽  
Natural Killer ◽  
Bone Marrow Involvement ◽  
Natural Killer Activity ◽  
Lymphocytic Leukemia ◽  
Leukemic Cells ◽  
Nonspecific Esterase ◽  
Low Grade ◽  
Killer Activity

Abstract A patient with low-grade lymphocytosis, splenomegaly, and neutropenia, but adequate myeloid leukogenesis, was found to have chronic lymphocytic leukemia, which represented a clonal proliferation of a distinct T-lymphocyte subset. The lymphocytes did not form E rosettes but had an OKT3+, OKT4+, OKT6+, OKT8+, OKT11+, HNK-1+, HNK-36+, OKIa1+, OKM1+ phenotype and functionally had suppressor and natural killer activity. Morphologically, they were large granular lymphocytes, which were strongly acid phosphatase positive and nonspecific esterase negative. They did not respond to mitogens, or to allogeneic cells. Initially, the spleen appeared to be the most involved organ and, judging from the high proportion of leukemic splenic lymphocytes in the S and G2/M phases of the cell cycle, was also the organ of origin of the leukemic cells. Only a few leukemic cells in the blood and bone marrow were in S and G2/M phases. After splenectomy, the lymphocyte count rose considerably and the bone marrow became progressively more infiltrated by tumor nodules. One year after diagnosis, the patient was started on chemotherapy because of progressive anemia. He responded to the chemotherapy by normalization of the hemoglobin and neutrophil count and had a moderate decrease in the bone marrow involvement and peripheral lymphocytosis.

scholarly journals Natural killer and suppressor T-cell chronic lymphocytic leukemia

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 627-634
Author(s):  
M Palutke ◽  
L Eisenberg ◽  
J Kaplan ◽  
M Hussain ◽  
K Kithier ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chronic Lymphocytic Leukemia ◽  
Natural Killer ◽  
Bone Marrow Involvement ◽  
Natural Killer Activity ◽  
Lymphocytic Leukemia ◽  
Leukemic Cells ◽  
Nonspecific Esterase ◽  
Low Grade ◽  
Killer Activity

A patient with low-grade lymphocytosis, splenomegaly, and neutropenia, but adequate myeloid leukogenesis, was found to have chronic lymphocytic leukemia, which represented a clonal proliferation of a distinct T-lymphocyte subset. The lymphocytes did not form E rosettes but had an OKT3+, OKT4+, OKT6+, OKT8+, OKT11+, HNK-1+, HNK-36+, OKIa1+, OKM1+ phenotype and functionally had suppressor and natural killer activity. Morphologically, they were large granular lymphocytes, which were strongly acid phosphatase positive and nonspecific esterase negative. They did not respond to mitogens, or to allogeneic cells. Initially, the spleen appeared to be the most involved organ and, judging from the high proportion of leukemic splenic lymphocytes in the S and G2/M phases of the cell cycle, was also the organ of origin of the leukemic cells. Only a few leukemic cells in the blood and bone marrow were in S and G2/M phases. After splenectomy, the lymphocyte count rose considerably and the bone marrow became progressively more infiltrated by tumor nodules. One year after diagnosis, the patient was started on chemotherapy because of progressive anemia. He responded to the chemotherapy by normalization of the hemoglobin and neutrophil count and had a moderate decrease in the bone marrow involvement and peripheral lymphocytosis.

scholarly journals Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry

Cytometry ◽  
1998 ◽  
Vol 32 (4) ◽  
pp. 280-285 ◽  
Author(s):  
Loris Zamai ◽  
Adriana R. Mariani ◽  
Giorgio Zauli ◽  
Luigi Rodella ◽  
Rita Rezzani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
K562 Cells ◽  
Killer Activity ◽  
Kinetics Of

Effect of serum factor(s) of endometriosis patients on natural killer activity

1989 ◽  
Vol 15 ◽  
pp. 194
Author(s):  
H.S. Wang ◽  
M. Kariya ◽  
H. Kanzaki ◽  
M. Yoshida ◽  
M. Tokushige ◽  
...  
Keyword(s):  
Natural Killer ◽  
Natural Killer Activity ◽  
Serum Factor ◽  
Killer Activity
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