An ultrastructural study of the culture forms of Trypanosoma (Schizotrypanum) myoti

1983 ◽  
Vol 61 (12) ◽  
pp. 2807-2815 ◽  
Author(s):  
W. Brockley Paterson ◽  
Patrick T. K. Woo
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Myotis Lucifugus ◽  
Basal Bodies ◽  
Kinetoplast Dna ◽  
Glutaraldehyde Fixation ◽  
Transmission Electron ◽  
Multiple Fission ◽  
Saline Medium

Trypanosoma myoti from the bat Myotis lucifugus was cultured in a diphasic blood-agar and saline medium and examined using transmission electron microscopy. Glutaraldehyde fixation revealed a pattern of kinetoplast DNA condensation in the epimastigote which is characteristic of the subgenus Schizotrypanum. Ferritin was used to demonstrate a functional cytostome–cytopharynx complex. Replication of the basal bodies, flagella, kinetoplast, and the nucleus during binary fission is described. Also present were cells which contain multiple sets of organelles and which undergo multiple fission. The ultrastructural features of Trypanosoma myoti resemble those of Trypanosoma cruzi.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

An Ultrastructural Study of Chronic Pyelonephritis in Macaca Arctoides

1976 ◽  
Vol 34 ◽  
pp. 310-311
Author(s):  
T.W. Smith ◽  
J.A. Roberts ◽  
B.J. Martin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Effective Means ◽  
Chronic Pyelonephritis ◽  
Macaca Arctoides ◽  
Left Ureter ◽  
E Coli ◽  
Transmission Electron ◽  
Sizeable Number

Chronic pyelonephritis is one of the most common diseases of the kidney and accounts for a sizeable number of cases of renal insufficiency in man, however its pathogenesis requires further elucidation. Transmission electron microscopy may serve as a uniquely effective means of observing details of the nature of this disease. The present paper describes preliminary results of an ultrastructural study of chronic pyelonephritis in Macaca arctoides (stumptail monkey).The infection was induced in these experiments in a retrograde fashion by means of a unilateral catheterization of the left ureter whereby an innoculum of 10 cc of broth containing approximately 2 billion E. coli per cc and radio-opaque dye were injected under pressure (mimicing vesico-ureteric reflux).

Spermiogenesis and sperm ultrastructure of Carmyerius endopapillatus (Digenea, Gastrothylacidae), a parasite of Bos taurus in Senegal

2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Momar Seck ◽  
Bernard Marchand ◽  
Cheikh Bâ
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Bos Taurus ◽  
Posterior Extremity ◽  
Sperm Ultrastructure ◽  
Lateral Expansion ◽  
Transmission Electron ◽  
Plasmic Membrane ◽  
Flagellar Rotation

AbstractUltrastructural study of spermiogenesis and of the spermatozoon of Carmyerius endopapillatus has enabled to describe some characteristics of this digenea. The intercentriolar body situated between the two striated roots and the two centrioles, presents a symmetric organization. Both external bands of this intercentriolar body are made up of a row of granules. During spermiogenesis, a flagellar rotation of 90° is described. The old spermatid does not present external ornamentations. The spermatozoon is characterized, in its anterior region, by the presence of a lateral expansion exhibiting one spinelike body. In C. endopapillatus, external ornamentations are localized only at the level where the lateral expansion appears. The posterior extremity of spermatozoon exhibits a nucleus surrounded by a plasmic membrane lacking microtubules, but presenting a small lateral expansion. This is the first species of Gastrothylacidae family studied by transmission electron microscopy.

Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Sequence Of Events ◽  
Transmission Electron ◽  
Dipetalonema Viteae ◽  
In Vitro Interaction

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

Ultrastructural study of spermatozoa of the paddlefish, Polyodon spathula

Zygote ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Otilia Zarnescu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Primitive Type ◽  
Polyodon Spathula ◽  
Transmission Electron ◽  
Architectural Features

Paddlefish, Polyodon spathula, spermatozoa were examined by transmission electron microscopy. Their structure has the same characteristic architectural features as sturgeon spermatozoa. Paddlefish spermatozoa are of the primitive type and consist of a rod-shaped head, a midpiece and a long flagellum. The head is about 5.15 mm in length and contains the nucleus and an apical acrosomal complex. Inside the nucleus there are three nuclear channels that begin in the subacrosomal area and have a triple helical arrangement. An nuclear fossa is present centrally, at the posterior end of the nucleus. The midpiece contains a pair of centrioles in a perpendicular arrangement, mitochondria and a narrow cytoplasmic sleeve. The flagellum has a central axoneme with a 9+2 pattern and two lateral projections or fins.

Paraliomyces lentiferus: an ultrastructural study of a little-known marine ascomycete

1992 ◽  
Vol 70 (11) ◽  
pp. 2223-2232 ◽  
Author(s):  
S. J. Read ◽  
S.-Y. Hsieh ◽  
E. B. G. Jones ◽  
S. T. Moss ◽  
H. S. Chang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Ultrastructural Study ◽  
Republic Of China ◽  
Mucilaginous Sheath ◽  
Transmission Electron ◽  
Scanning Electron

A collection of Paraliomyces lentiferus from Taiwan, Republic of China, is compared with that of the type description and examined at both scanning and transmission electron microscope levels as part of our review of the taxonomy of the marine Ascomycotina. Particular attention was devoted to the structure of the ascospore appendage. The ascospore wall comprises a mesosporium, an episporium, and a mucilaginous sheath (exosporium?) In addition, there is a single, gelatinous, lateral appendage adjacent to the central septum. The appendage comprises electron-opaque fibrils that in immature ascospores are connected to the ascospore wall via fine electron-opaque strands and larger electron-opaque aggregates of material. The origin of the appendage is discussed. Key words: ascospore, attachment, marine ascomycete, scanning electron microscopy, spore appendage, transmission electron microscopy.

An ultrastructural study of reproduction in the parthenogenetic metacercariae of Cercaria margaritensis Ching, 1982 (Digenea: Gymnophallidae)

Parasitology ◽  
2003 ◽  
Vol 126 (3) ◽  
pp. 261-271 ◽  
Author(s):  
S. W. B. IRWIN ◽  
K. V. GALAKTIONOV ◽  
I. I. MALKOVA ◽  
D. H. SAVILLE ◽  
S. M. FITZPATRICK
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Embryo Development ◽  
Ultrastructural Study ◽  
Definitive Host ◽  
Parent Body ◽  
Common Eider ◽  
Somateria Mollissima ◽  
Transmission Electron ◽  
The Common

The parthenogenetic metacercarial stages of the gymnophallid trematode Cercaria margaritensis are found in the extrapallial cavity of the subtidal prosobranch mollusc Margarites helicinus. The primary metacercariae (M1) produce second-generation metacercariae (M2) which become independent and give rise to M3 metacercariae which are infective to the definitive host, the common eider (Somateria mollissima). This study used transmission electron microscopy to follow the development of M2 inside M1 organisms and M3 inside M2 organisms. The process is similar in both cases with embryos developing from individual cells from the parent body walls. In each case the brood sac was divided into brood chambers by multilaminated cells and both M2 and M3 embryos developed inside embryonic membranes that originated from specialized blastomeres. The tegument of M2 and M3 embryos developed in a similar manner underneath the embryonic membrane. Both the multilaminated cells and the embryonic membranes possessed features that indicated that they are involved in transport of nutrients. It is suggested that the continuous nature of M2 and M3 embryo development may well be similar to that postulated for ancestral digeneans.

Ultrastructural study of frontal glands in Bothromesostoma personatum (Platyhelminthes, Typhloplanoida)

1994 ◽  
Vol 72 (4) ◽  
pp. 707-711
Author(s):  
S. Martínez-Alós ◽  
P. García-Corrales ◽  
B. Cifrian
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Formation Mechanism ◽  
Ultrastructural Study ◽  
Gland Cell ◽  
The Other ◽  
Gland Cells ◽  
Transmission Electron

The frontal glands of Bothromesostoma personatum were examined using transmission electron microscopy. These glands have two types of rhabdoid gland cells; one type produces rhabdites and the other a different type of rod secretion (rhabdoids). The ultrastructure of the latter type is described, together with their origin. Long necks containing the rhabdoids emerge from the rhabdoid gland cell bodies and extend to the anterior end of the animal, where they release the rhabdoids. The rhabdoids are compared with the rhammites of other rhabditophoran species on the basis of their dye affinities, formation mechanism, and ultrastructure.

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