Ultrastructural study of frontal glands in Bothromesostoma personatum (Platyhelminthes, Typhloplanoida)

1994 ◽  
Vol 72 (4) ◽  
pp. 707-711
Author(s):  
S. Martínez-Alós ◽  
P. García-Corrales ◽  
B. Cifrian

The frontal glands of Bothromesostoma personatum were examined using transmission electron microscopy. These glands have two types of rhabdoid gland cells; one type produces rhabdites and the other a different type of rod secretion (rhabdoids). The ultrastructure of the latter type is described, together with their origin. Long necks containing the rhabdoids emerge from the rhabdoid gland cell bodies and extend to the anterior end of the animal, where they release the rhabdoids. The rhabdoids are compared with the rhammites of other rhabditophoran species on the basis of their dye affinities, formation mechanism, and ultrastructure.

Author(s):  
Bruce Mackay

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.


Author(s):  
T.W. Smith ◽  
J.A. Roberts ◽  
B.J. Martin

Chronic pyelonephritis is one of the most common diseases of the kidney and accounts for a sizeable number of cases of renal insufficiency in man, however its pathogenesis requires further elucidation. Transmission electron microscopy may serve as a uniquely effective means of observing details of the nature of this disease. The present paper describes preliminary results of an ultrastructural study of chronic pyelonephritis in Macaca arctoides (stumptail monkey).The infection was induced in these experiments in a retrograde fashion by means of a unilateral catheterization of the left ureter whereby an innoculum of 10 cc of broth containing approximately 2 billion E. coli per cc and radio-opaque dye were injected under pressure (mimicing vesico-ureteric reflux).


2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Momar Seck ◽  
Bernard Marchand ◽  
Cheikh Bâ

AbstractUltrastructural study of spermiogenesis and of the spermatozoon of Carmyerius endopapillatus has enabled to describe some characteristics of this digenea. The intercentriolar body situated between the two striated roots and the two centrioles, presents a symmetric organization. Both external bands of this intercentriolar body are made up of a row of granules. During spermiogenesis, a flagellar rotation of 90° is described. The old spermatid does not present external ornamentations. The spermatozoon is characterized, in its anterior region, by the presence of a lateral expansion exhibiting one spinelike body. In C. endopapillatus, external ornamentations are localized only at the level where the lateral expansion appears. The posterior extremity of spermatozoon exhibits a nucleus surrounded by a plasmic membrane lacking microtubules, but presenting a small lateral expansion. This is the first species of Gastrothylacidae family studied by transmission electron microscopy.


1994 ◽  
Vol 72 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Georgia L. Hoffman ◽  
Ruth A. Stockey

Several hundred vegetative and fertile specimens of Azolla Lam. have been recovered from the Paleocene Paskapoo Formation at the Joffre Bridge locality (Middle Tiffanian (Ti3) age) near Red Deer, Alberta. The spore complexes closely resemble those of the Paleocene A. stanleyi Jain & Hall, and the vegetative material is referred to that species. The specimens are unusually complete in that the remains of the fragile sporophyte are preserved, commonly with reproductive structures in place. Plants reaching up to 2.25 cm in length consist of alternately branched rhizomes bearing alternate, imbricate, sessile leaves. Leaves are ovate with entire margins, papillate surfaces, and a single midvein. Reproductive structures have been examined using light, scanning, and transmission electron microscopy. This new material is compared with the other Paleocene species for which sporophytes are known and discussed in terms of evolutionary trends for the genus. The specimens suggest that most of the vegetative characteristics of modern Azolla species were established by the middle Paleocene. Key words: Azolla, Salviniaceae, megaspore, massula, ultrastructure, Paleocene.


Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 


Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.


Zygote ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Otilia Zarnescu

Paddlefish, Polyodon spathula, spermatozoa were examined by transmission electron microscopy. Their structure has the same characteristic architectural features as sturgeon spermatozoa. Paddlefish spermatozoa are of the primitive type and consist of a rod-shaped head, a midpiece and a long flagellum. The head is about 5.15 mm in length and contains the nucleus and an apical acrosomal complex. Inside the nucleus there are three nuclear channels that begin in the subacrosomal area and have a triple helical arrangement. An nuclear fossa is present centrally, at the posterior end of the nucleus. The midpiece contains a pair of centrioles in a perpendicular arrangement, mitochondria and a narrow cytoplasmic sleeve. The flagellum has a central axoneme with a 9+2 pattern and two lateral projections or fins.


1987 ◽  
Vol 94 ◽  
Author(s):  
S. W. Lu ◽  
C. W. Nieh ◽  
J. J. Chu ◽  
L. J. Chen

ABSTRACTThe influences of implantation impurities, including BF2, B, F, As and P on the formation of epitaxial NiSi2 in nickel thin films on ion-implanted silicon have been investigated by transmission electron microscopy.The presence of BF2, B, and F atoms was observed to promote the epitaxial growth of NiSi2 at low temperatures. Little or no effect on the formation of NiSi2 was found in samples implanted with As or P ions.The results indicated that the influences of the implantation impurities are not likely to be electronic in origin. Good correlation, on the other hand, was found between the atomic size factor and resulting stress and NiSi2 epitaxy at low temperatures.


Author(s):  
Sam Ick Son ◽  
Su Jin Chung

AbstractThe relation between the domains and domain boundaries of multiple twins of diamond were investigated by the electron back scatter diffraction (EBSD) method and high resolution transmission electron microscopy (HRTEM). Multiple twinned diamonds have two types of icosahedral morphologies. One is an almost perfect icosahedron in which all of the faces are {111} faces. The other is a hollow icosahedron similar to one of the Kepler-Poinsot polyhedrons. The indented negative trigonal faces are formed from the {100} faces of a cube. It was confirmed that the convex edges of the twinned icosahedron corresponded to the Σ3 boundaries, whereas the concave edges were assigned to the Σ9 twin boundary by means of the EBSD analysis.It was confirmed from the HRTEM image that a series of dislocations compensate for the mismatching angle which occurs after five successive twinning.


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